Os02g0649300 - Revision history

Xysj1988: /* Annotated Information */

2016-10-03T11:34:11Z

‎Annotated Information

Xysj1988: /* Subcellular Localization */

2016-10-03T11:33:48Z

‎Subcellular Localization

Xysj1988: /* Function */

2016-10-03T11:31:21Z

‎Function

Xysj1988: /* Expression */

2016-10-03T11:30:55Z

‎Expression

Xysj1988 at 11:27, 3 October 2016

2016-10-03T11:27:18Z

Xysj1988: /* Expression */

2016-10-03T11:15:57Z

‎Expression

Xysj1988: /* Expression */

2016-10-03T11:15:41Z

‎Expression

Xysj1988: /* Expression */

2016-10-03T11:14:41Z

‎Expression

Xysj1988: /* Expression */

2016-10-03T11:14:27Z

‎Expression

Xysj1988: /* References */

2016-10-03T11:11:09Z

‎References

@@ Line 4: / Line 4: @@
 ===Gene Symbol===
 *'''''Os02g0649300''''' '''''<=>''''' '''''OsSLI1'''''
+[[File:7-Os02g0649300-1.png|right|thumb|357px|'''Figure 6.''' ''Nuclear localization of OsSLI1-GFP fusion protein.<ref name="ref1" />.'']]
 ===Function===
 * '''''OsSLI1''''' encodes member of the HD-Zip I subfamily
@@ Line 17: / Line 17: @@
 ===Subcellular Localization===
 * '''''OsSLI1''''' is a nuclear-localized protein, in consistency with its potential function as a DNA binding transcription factor.
-[[File:7-Os02g0649300-1.png|right|thumb|357px|'''Figure 6.''' ''Nuclear localization of OsSLI1-GFP fusion protein.<ref name="ref1" />.'']]
 You can also add sub-section(s) at will.

@@ Line 17: / Line 17: @@
 ===Subcellular Localization===
 * '''''OsSLI1''''' is a nuclear-localized protein, in consistency with its potential function as a DNA binding transcription factor.
-[[File:6-Os02g0649300-1.png|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications
+[[File:7-Os02g0649300-1.png|right|thumb|357px|'''Figure 6.''' ''Nuclear localization of OsSLI1-GFP fusion protein.<ref name="ref1" />.'']]
 You can also add sub-section(s) at will.

@@ Line 8: / Line 8: @@
 * '''''OsSLI1''''' encodes member of the HD-Zip I subfamily
 * '''''OsSLI1''''' may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
 ===Expression===

@@ Line 10: / Line 10: @@
 ===Expression===
-* Quantitative RT-PCR result showed that, in the seedlings, '''''OsSLI1''''' expression was mainly in root.
+* Quantitative RT-PCR result showed that in the seedlings, '''''OsSLI1''''' expression was mainly in root.
 * The root tissue accumulates 7 times of '''''OsSLI1''''' transcripts than in shoot tissue.
-* In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue
+* In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue.
+* Quantitative RT-PCR assay showed that OsSLI1 expression was highly induced by salt, PEG, H2O2 , high temperature, and ABA treatment but not by cold treatment.
 [[File:6-Os02g0649300-1.png|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications
 ± standard error. One independent plant was considered as one biological replication.<ref name="ref1" />.'']]

@@ Line 1: / Line 1: @@
-The rice '''''Os02g0649300''''' was reported as '''''OsEXPA3''''' in 2014 <ref name="ref1" /> by researchers from China.
+The rice '''''Os02g0649300''''' was reported as '''''OsSLI1''''' in 2014 <ref name="ref1" /> by researchers from China.
 ==Annotated Information==
 ===Gene Symbol===
-*'''''Os02g0649300''''' '''''<=>''''' '''''OsEXPA3'''''
+*'''''Os02g0649300''''' '''''<=>''''' '''''OsSLI1'''''
 ===Function===
-* '''''OsEXPA3''''' is required for root system development in rice and involved in mediating root growth by mediating cell-wall loosening
+* '''''OsSLI1''''' encodes member of the HD-Zip I subfamily
-* '''''OsEXPA3''''' is a critical root expansin gene that is intrinsically involved in root system architecture adapted to some abiotic stresses.
+* '''''OsSLI1''''' may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.
 ===Expression===
-* Semiquantitative-PCR analysis demonstrated that '''''OsEXPA3''''' expressed preferentially in the root of 1-wk- old seedlings.
+* Quantitative RT-PCR result showed that, in the seedlings, '''''OsSLI1''''' expression was mainly in root.
-* Expressing preferentially in the root throughout the whole developmental stages, '''''OsEXPA3''''' displayed the expression peak in 1-wk-old seedlings but decreased drastically at the tillering stage, and the expression diminution continued with the passage of growth time.
+* The root tissue accumulates 7 times of '''''OsSLI1''''' transcripts than in shoot tissue.
-* As to abiotic stresses and nutrient deficiency, '''''OsEXPA3''''' expression in the root was significantly upregulated by NaCl stress, while repressed by N and Pi deficiency. The result implies an intrinsic function of '''''OsEXPA3''''' in regulation of the root system architecture in response to abiotic stresses.<br><br>
+* In panicles, the expression changes in different development stages, from relative low in 3 cm young panicles to a 33-fold increase in 8 cm panicles, and the 12 cm panicles still accumulate 8-fold OsSLI1 transcripts compared to 3 cm young panicles tissue
 [[File:6-Os02g0649300-1.png|center|thumb|800px|'''Figure 3.''' ''Effects of OsEXPA3 downregulation on the root system architecture. (a) Primary root length of 1-wk-old seedlings. (b) Lateralroot density of 1-wk-old seedlings. Two independent transgenic lines (Line 1 and Line 3) were analyzed. The seeds were placed on half-strength Murashige and Skoog solid medium and grown for 7 d vertically in a growth chamber. Primary root length was determined using a ruler. All visible lateral roots that emerged from the primary root were counted. Values are the means of 20 biological replications
@@ Line 21: / Line 20: @@
 ==Labs working on this gene==
-* Key Lab. of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing Univ., Chongqing, 400030, P. R. China.
+* State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
 ==References==
 <references>
 * <ref name="ref1">
-Qiu, Shichun, et al. "Repression of Expression Leads to Root System Growth Suppression in Rice." Crop Science 54.5 (2014): 2201-2213.
+Huang X, Duan M, Liao J, Yuan X, Chen H, Feng J, Huang J, Zhang HS. OsSLI1, a  homeodomain containing transcription activator, involves abscisic acid related stress response in rice (Oryza sativa L.). ScientificWorldJournal. 2014;2014:809353. doi: 10.1155/2014/809353. Epub 2014 Jun 25. PubMed PMID: 25089296; PubMed Central PMCID: PMC4095735.
 </references>
 ==Structured Information==
       [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 2]]