Os02g0649900 - Revision history

Xysj2012: /* Structured Information */

2015-05-14T05:48:39Z

‎Structured Information

Eric Fu: /* Expression */

2014-05-19T16:23:50Z

‎Expression

Eric Fu at 16:21, 19 May 2014

2014-05-19T16:21:08Z

Eric Fu: /* Function */

2014-05-19T15:50:05Z

‎Function

Eric Fu at 13:51, 19 May 2014

2014-05-19T13:51:39Z

Eric Fu at 13:16, 19 May 2014

2014-05-19T13:16:56Z

Eric Fu at 12:42, 19 May 2014

2014-05-19T12:42:52Z

Eric Fu at 10:10, 19 May 2014

2014-05-19T10:10:56Z

Eric Fu: /* Expression */

2014-05-19T10:04:01Z

‎Expression

Eric Fu: /* References */

2014-05-19T10:00:29Z

‎References

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 20: / Line 20: @@
 *In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.
-  [[File:Fig.3 OsYSL2-3.gif|right|thumb|250px|'''Figure 3.''' ''The unrooted phylogenic tree for the 18 OsYSL amino acid sequences.(From reference <ref name="ref1" />).'']]
+  [[File:Fig.3 OsYSL2-3.png|right|thumb|250px|'''Figure 3.''' ''The unrooted phylogenic tree for the 18 OsYSL amino acid sequences.(From reference <ref name="ref1" />).'']]
 ===Evolution===
 YS1-like genes in rice Our search for YS1 homologus in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified

@@ Line 2: / Line 2: @@
 ==Annotated Information==
 ===Function===
-OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.
+*OsYSL2 is an Fe-regulated metal-NA transporter that is involved in phloem transport and the translocation of mineral nutrients in grains<ref name="ref1" />. The altered expression of OsYSL2changes the localization of Fe, and that OsYSL2 is a critical Fe-nicotianamine transporter important for Fe translocation,especially in the shoots and endosperm <ref name="ref2" />.
-Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.
+*Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.
-[[File:Fig.1 OsYSL2.gif|right|thumb|250px|Fig.1 Expression and seed iron (Fe) concentrations of OsYSL2i and phenotype of OsYSL2i at the early growth stage.(From reference <ref name="ref2" />).'']]
+[[File:Fig.1 OsYSL2.gif|right|thumb|250px|'''Figure 1.''' ''Expression and seed iron (Fe) concentrations of OsYSL2i and phenotype of OsYSL2i at the early growth stage.(From reference <ref name="ref2" />).'']]
 ===Mutant===
-*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis  . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions <ref name="ref2" />.
+*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis(Figure 1a). Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 1c–e)<ref name="ref2" />.
 *Yasuhiro Ishimaru et al. assessed whether the over expression of OsYSL2(OXOsYSL2) in rice plants could enhance Fe accumulation in the seeds. Constitutive over expression of OsYSL2 decreased the Fe concentration in the brown rice and shoots ,although the Fe concentration was higher in the roots of OXOsYSL2 plants. On the other hand, the Mn concentration was increased in the brown rice and decreased in the roots of OXOsYSL2 plants<ref name="ref2" />.
 ===Expression===
 *OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al.  showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
-*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
+*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development(figure 2)<ref name="ref1" />.
 *In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.
 ===Evolution===
-YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
+YS1-like genes in rice Our search for YS1 homologus in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
-putative OsYSL that exhibited 36–76% sequence similarity to YS1 <ref name="ref1" />.
+putative OsYSL that exhibited 36–76% sequence similarity to YS1(figure 3) <ref name="ref1" />.
 ==Labs working on this gene==

← Older revision		Revision as of 15:50, 19 May 2014
Line 5:		Line 5:

	Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.		Based on the nucleotide sequence,OsYSL2 was predicted to encode a polypeptide of 674 amino acids containing 14 putative transmembrane domains<ref name="ref1" />. Cells expressing the OsYSL2–GFP fusion protein showed a signal in the plasma membrane, with the presence of the putative transmembrane domains shows that OsYSL2 is a transporter that is localized in the plasma membrane<ref name="ref1" />.
		+	[[File:Fig.1 OsYSL2.gif\|right\|thumb\|250px\|Fig.1 Expression and seed iron (Fe) concentrations of OsYSL2i and phenotype of OsYSL2i at the early growth stage.(From reference <ref name="ref2" />).'']]

	===Mutant===		===Mutant===

@@ Line 7: / Line 7: @@
 ===Mutant===
-*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis (Figure 1a) . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions (Figure 2c–e)<ref name="ref2" />.
+*Yasuhiro Ishimaru et al. produced OsYSL2 knock-down lines using the RNAi method. Seven independent lines were generated, and the expression of endogenous OsYSL2 in T1OsYSL2i leaves of lines 3 and 5 was suppressed, as revealed by northern blot analysis  . Fe concentrations in the seeds were also decreased in lines 3 and 5,consistent with the suppression of OsYSL2(Figure 1b).At the early growth stage, the growth of line 5 was impaired compared with the WT, when grown under Fe-sufficient conditions. These growth defects appeared to be more significant when grown under Fe-deficient conditions <ref name="ref2" />.
@@ Line 14: / Line 14: @@
 ===Expression===
 *OsYSL2 was of particular interest because no detectable transcripts were present in the roots of either Fe-sufficient or Fe-deficient plants, but dramatic expression was induced in the leaves of Fe-deficient plants<ref name="ref1" />.Research by Yasuhiro Ishimaru et al.  showed that the high expression of OsYSL2 in the roots under Fe deficiency was not consistent with previous study<ref name="ref2" />.
-*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice(Figure 2e–g). The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
+*The cell-type specificity of OsYSL2 expression was investigated using promoter (1.5 kb):b-glucuronidase (GUS) analysis.GUS staining was observed in phloem cells of the vascular bundles of leaves and leaf sheaths of Fe-sufficient rice. The phloem-specific expression of the OsYSL2 promoter suggested that OsYSL2 is involved in the phloem transport of Fe <ref name="ref1" />. OsYSL2 is expressed during reproductive development in rice. RT-PCR analysis confirmed that OsYSL2 transcripts were increased during early seed development<ref name="ref1" />.
 *In fully mature seeds,strong OsYSL2 expression was observed in the epithelium, the vascular bundle of the scutellum, and the leaf primordium . Two days after sowing, the OsYSL2 expression level increased in the scutellum near the endosperm. Three days after sowing,OsYSL2 expression was observed in the bran and coleoptile<ref name="ref3" />.
 ===Evolution===
 YS1-like genes in rice Our search for YS1homologues in the Oryza sativaL. Ssp. japonica (cv. Nipponbare) rice genomic database identified
-putative OsYSL that exhibited 36–76% sequence similarity to YS1 (Figure 3)<ref name="ref1" />.
+putative OsYSL that exhibited 36–76% sequence similarity to YS1 <ref name="ref1" />.
 ==Labs working on this gene==

@@ Line 20: / Line 20: @@
 ==References==
 <references>
-<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID: 15255870</ref>
+<ref name="ref1"> Koike S, Inoue H, Mizuno D, et al. OsYSL2 is a rice metal‐nicotianamine transporter that is regulated by iron and expressed in the phloem[J]. The Plant Journal, 2004, 39(3): 415-424.PMID 15255870</ref>
-<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID: 20128878</ref>
+<ref name="ref2"> Ishimaru Y, Masuda H, Bashir K, et al. Rice metal‐nicotianamine transporter, OsYSL2, is required for the long‐distance transport of iron and manganese[J]. The Plant Journal, 2010, 62(3): 379-390.PMID 20128878</ref>
-<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID: 17333504</ref>
+<ref name="ref3"> Nozoye T, Inoue H, Takahashi M, et al. The expression of iron homeostasis-related genes during rice germination[J]. Plant molecular biology, 2007, 64(1-2): 35-47.PMID 17333504</ref>
 </references>