Os02g0799100 - Revision history

Xysj2012: /* Structured Information */

2015-05-14T06:02:03Z

‎Structured Information

Zhangziliang: /* Evolution */

2014-06-01T05:09:46Z

‎Evolution

Zhangziliang: /* Expression（Mutant VS. Wild type） */

2014-06-01T05:09:26Z

‎Expression（Mutant VS. Wild type）

Zhangziliang: /* Mutation */

2014-06-01T05:08:32Z

‎Mutation

Zhangziliang: /* Mutation */

2014-06-01T05:08:09Z

‎Mutation

Zhangziliang: /* Labs working on this gene */

2014-06-01T05:06:56Z

‎Labs working on this gene

Zhangziliang: /* Expression（Mutant VS Wild type） */

2014-06-01T02:54:46Z

‎Expression（Mutant VS Wild type）

Zhangziliang: /* Labs working on this gene */

2014-06-01T02:49:21Z

‎Labs working on this gene

Zhangziliang: /* Labs working on this gene */

2014-06-01T02:48:37Z

‎Labs working on this gene

Zhangziliang: /* Knowledge Extension */

2014-06-01T02:39:45Z

‎Knowledge Extension

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Evolution===
-[[File:Figure S2.jpg|right|thumb|250px|''Alignment of OsSGO1 with ZmSGO1 in maize (from reference <ref name="ref2" />).'']]
+[[File:Figure S2.jpg|right|thumb|300px|''Alignment of OsSGO1 with ZmSGO1 in maize (from reference <ref name="ref2" />).'']]
 ''SGO1'' gene is the first meiosis specificity expressed gene in yeast cDNA library screening conducted in Watanabe Lab, as well as to the lack of corresponding mutant phenotype analysis, identified as the homologous gene of Drosophila melanogaster Mei - S332 <ref name="ref4" /><ref name="ref5" />. Many researches of SGO function have been investigated in animals and yeast <ref name="ref6" />, while relatively few has been reported in plants. In plants, ''SGO1'' gene is first found in maize, and the mutant of premature-dissociation centromeric cohesion which locates in the centromere at the stage of anaphase Ⅰ. And the phenotype is due to the deletion of ZmSGO1 protein. ZmSGO1 is found to be located in the centromere from the eptotene stage Previous studies indicates that ZmSGO1 plays an important role in protecting of centromeric cohesion during meiosis I <ref name="ref7" />. The OsSGO1 protein sequence,which comprises 486 amino acid residues, shows a high similarity toZmSGO1(209/537 residues identical and 278/537 residues positive(Figure S2)<ref name="ref2" />.

@@ Line 10: / Line 10: @@
 ===Expression（Mutant VS. Wild type）===
-[[File:FigureS2.jpg|right|thumb|250px|''Expression analysis of OsSGO1 (from reference <ref name="ref2" />).'']]
+[[File:FigureS2.jpg|right|thumb|300px|''Expression analysis of OsSGO1 (from reference <ref name="ref2" />).'']]
-[[File:FigureS3.jpg|right|thumb|250px|''Characterization of the phenotype of ''Ossgo1'' mutants (from reference <ref name="ref2" />).'']]
+[[File:FigureS3.jpg|right|thumb|300px|''Characterization of the phenotype of ''Ossgo1'' mutants (from reference <ref name="ref2" />).'']]
-[[File:FigureS4.jpg|right|thumb|250px|''The defective cDNA sequence from ''Ossgo1-1'' mutant (from reference <ref name="ref2" />).'']]
+[[File:FigureS4.jpg|right|thumb|300px|''The defective cDNA sequence from ''Ossgo1-1'' mutant (from reference <ref name="ref2" />).'']]
-[[File:FigureS5.jpg|right|thumb|250px|''Western blotting analysis of OsSGO1 expression (from reference <ref name="ref2" />).'']]
+[[File:FigureS5.jpg|right|thumb|300px|''Western blotting analysis of OsSGO1 expression (from reference <ref name="ref2" />).'']]
 ''OsSGO1'' is expressed most highly in the roots, secondly in the panicles, and at a relatively low level in leaves(Figure S3)<ref name="ref2" />.

@@ Line 6: / Line 6: @@
 ===Mutation===
-[[File:Figure S1.jpg|left|thumb|300px|''Schematic representation of OsSGO1 gene (from reference <ref name="ref2" />).'']]
+[[File:Figure S1.jpg|right|thumb|300px|''Schematic representation of OsSGO1 gene (from reference <ref name="ref2" />).'']]
 An OsSGO1-RNAi vector is transformed into rice calli by Agrobacterium-mediated DNA transfer. OsSGO1-RNAi lines is obtained by screening the plants regenerated from the transformed calli by PCR. One single ''Tos17''-insertion mutant line of the ''OsSGO1'' gene, named ''Ossgo1-1'', is identified by screening the public insertion line collections. Sequence analysis of its PCR products confirm that ''Tos17'' is inserted into exon 15, only 5 bp upstream from the stop codon(Figure S1). <ref name="ref2" /> .

@@ Line 6: / Line 6: @@
 ===Mutation===
-[[File:Figure S1.jpg|right|thumb|250px|''Schematic representation of OsSGO1 gene (from reference <ref name="ref2" />).'']]
+[[File:Figure S1.jpg|left|thumb|300px|''Schematic representation of OsSGO1 gene (from reference <ref name="ref2" />).'']]
 An OsSGO1-RNAi vector is transformed into rice calli by Agrobacterium-mediated DNA transfer. OsSGO1-RNAi lines is obtained by screening the plants regenerated from the transformed calli by PCR. One single ''Tos17''-insertion mutant line of the ''OsSGO1'' gene, named ''Ossgo1-1'', is identified by screening the public insertion line collections. Sequence analysis of its PCR products confirm that ''Tos17'' is inserted into exon 15, only 5 bp upstream from the stop codon(Figure S1). <ref name="ref2" /> .

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 *State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China
 *Yangzhou University, Yangzhou 225009, Jiangsu Province； Institute of Genetics,Chinese Academy of Sciences, Beijing 100101
 ==References==

@@ Line 51: / Line 51: @@
 *State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China
 *Yangzhou University, Yangzhou 225009, Jiangsu Province； Institute of Genetics,Chinese Academy of Sciences, Beijing 100101
-*Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, and 2SORST, Japan Science and Technology Agency, Hongo,
+*Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, and 2SORST, Japan Science and Technology Agency, Hongo, Tokyo 113-0033, Japan
 *Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge,Massachusetts 02139, USA
 *University of Colorado Health Sciences Center, Aurora, Colorado 80010, USA

← Older revision		Revision as of 02:48, 1 June 2014
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	*State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China		*State Key Laboratory of Plant Genomics and Center for Plant Gene Research, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, Beijing 100101, China
		+	*Yangzhou University, Yangzhou 225009, Jiangsu Province； Institute of Genetics,Chinese Academy of Sciences, Beijing 100101
		+	*Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, and 2SORST, Japan Science and Technology Agency, Hongo,
		+	Tokyo 113-0033, Japan
		+	*Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge,Massachusetts 02139, USA
		+	*University of Colorado Health Sciences Center, Aurora, Colorado 80010, USA
		+	*Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
		+	*Department of Molecular and Cellular Biology,Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
		+	*Department of Molecular and Cell Biology University of California, Berkeley Berkeley, California 94720

	==References==		==References==

@@ Line 45: / Line 45: @@
 ===Knowledge Extension===
-OsSGO1 is a shugoshin protein. Shugoshin is a conserved protein in eukaryotes that protects the centromeric cohesin of sister chromatids from cleavage by separase during meiosis<ref name="ref2" />. Shugoshins (Japanese for ‘guardian spirit’) are the conserved proteins required to protect the cohesin adjacent to the centromeres from cleavage by separase. ''Drosophila'' Mei-S332 was the first protein in the shugoshin family to be discovered， which  is necessary for chromosome precise separation <ref name="ref4" /><ref name="ref8" /><ref name="ref9" />. From then, shugoshins have been identified in various organisms from yeast to humans. ''Saccharomyces cerevisiae'' and ''Drosophila'' have only one kinds of shugoshin, which is expressed in both mitosis and meiosis, while ''Schizosaccharomyces pombe'' and mammals have two (Sgo1 and Sgo2) <ref name="ref4" /><ref name="ref10" />. There are two conserved domains in shugoshin proteins: one is the coiled-coil that is located near the N-terminal, which may mediate homodimerization and interactions with other proteins; the other is the basic region in the C-terminal, which is required for chromosomal localization<ref name="ref4" /><ref name="ref11" />. The activity of shugoshin is controlled by an accurate and complicated process during cell divisions. In ''S. pombe'', the location of Sgo1 is regulated by the kinase, Bub1, and its degradation at anaphase I is controlled by the anaphasepromoting complex (APC)<ref name="ref4" />.In plants, such as rice and maize, SGO1 is loaded onto  the chromosome during leptotene, which is much earlier thanthat in other organisms, and it disassociates during anaphase I. ZmSGO1 is reported to localize at both the centromere core and the pericentromeric regions<ref name="ref7" />.
+OsSGO1 is a shugoshin protein. Shugoshin is a conserved protein in eukaryotes that protects the centromeric cohesin of sister chromatids from cleavage by separase during meiosis<ref name="ref2" />. Shugoshins (Japanese for ‘guardian spirit’) are the conserved proteins required to protect the cohesin adjacent to the centromeres from cleavage by separase. ''Drosophila'' Mei-S332 is the first protein in the shugoshin family to be discovered， which  is necessary for chromosome precise separation <ref name="ref4" /><ref name="ref8" /><ref name="ref9" />. From then, shugoshins have been identified in various organisms from yeast to humans. ''Saccharomyces cerevisiae'' and ''Drosophila'' have only one kinds of shugoshin, which is expressed in both mitosis and meiosis, while ''Schizosaccharomyces pombe'' and mammals have two (Sgo1 and Sgo2) <ref name="ref4" /><ref name="ref10" />. There are two conserved domains in shugoshin proteins: one  is the basic region in the C-terminal, which is required for chromosomal localization; the other is the coiled-coil that is located near the N-terminal, which may mediate homodimerization and interactions with other proteins<ref name="ref4" /><ref name="ref11" />. The activity of shugoshin is controlled by an accurate and complicated process during cell divisions. In ''S. pombe'', the location of Sgo1 is regulated by the kinase, Bub1, and its degradation at anaphase I is controlled by the anaphasepromoting complex (APC)<ref name="ref4" />.SGO1 is loaded onto  the chromosome during leptotene, which is much earlier than that in other organisms,in plants such as rice and maize, and it disassociates during anaphase I. ZmSGO1 is reported to localize at both the centromere core and the pericentromeric regions<ref name="ref7" />.
 ==Labs working on this gene==