Os03g0100800 - Revision history

192.168.72.52: /* Structured Information */

2015-05-15T05:39:09Z

‎Structured Information

Shijc at 16:22, 4 June 2014

2014-06-04T16:22:52Z

Shijc at 16:06, 4 June 2014

2014-06-04T16:06:45Z

Shijc at 16:06, 4 June 2014

2014-06-04T16:06:19Z

Shijc at 16:05, 4 June 2014

2014-06-04T16:05:37Z

Shijc at 16:03, 4 June 2014

2014-06-04T16:03:35Z

Shijc at 03:52, 2 June 2014

2014-06-02T03:52:36Z

Helen: /* Labs working on this gene */

2014-06-01T10:19:59Z

‎Labs working on this gene

Helen: /* Labs working on this gene */

2014-06-01T10:19:01Z

‎Labs working on this gene

Helen: /* Labs working on this gene */

2014-06-01T10:11:27Z

‎Labs working on this gene

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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-OsA8 encodes a typical P-type ATPase-like protein of rice.
+''OsA8'' encodes a typical P-type ATPase-like protein of rice.
 ==Annotated Information==
 ===Function===
-[[File: Shijc-Os03g0100800-Fig1.jpg|right|thumb|200px|''Comparison of the Osa8 mutant and wild-type seedlings grown under phosphate starvation for 2 weeks. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig1.jpg|right|thumb|200px|''Comparison of the ''Osa8'' mutant and wild-type seedlings grown under phosphate starvation for 2 weeks. (from reference <ref name="ref7" />).'']]
 As a primary transporter, P-type H+-ATPases (P3A) mediate ATP-dependent H+ extrusion to the extracellular space, a process that generates pH and electrical potential difference across the PM <ref name="ref1" /><ref name="ref2" />, and this difference works as the motive force for a large set of secondary transporters, including symporter, antiporter, or uniporter, to drive their substrates against their concentration gradients. <ref name="ref3" /><ref name="ref4" /><ref name="ref5" /> Therefore, the H+-ATPase acts as a transducer: converting the chemical energy released from ATP hydrolysis into chemiosmotic energy. <ref name="ref1" />
-The basic function of P-type H+-ATPases is the coupling ATP hydrolysis and H+-pumping. In general, the cells undergoing active metabolism have much higher activity of H+-ATPase than others (Palmgren, 2001; Arango et al., 2003; Sondergaard et al., 2004). <ref name="ref3" /><ref name="ref1" /><ref name="ref6" />
+The basic function of P-type H+-ATPases is the coupling ATP hydrolysis and H+-pumping. In general, the cells undergoing active metabolism have much higher activity of H+-ATPase than others. <ref name="ref3" /><ref name="ref1" /><ref name="ref6" />
 ===Expression===
-[[File: Shijc-Os03g0100800-Fig2.jpg|right|thumb|200px|''Relative expressional level of the OsA8 gene in roots and leaves of wild-type rice grown under different nutrient conditions examined by real-time RT-PCR. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig2.jpg|right|thumb|200px|''Relative expressional level of the ''OsA8'' gene in roots and leaves of wild-type rice grown under different nutrient conditions examined by real-time RT-PCR. (from reference <ref name="ref7" />).'']]
-Expression pattern of OsA8 is investigated in rice seedlings. Transcripts of OsA8 accumulated more abundantly in roots than in leaves under normal growing condition. OsA8 expression is suppressed in both roots and leaves under N, P, and K deficiency, indicating a much broader role of it in nutrient acquisition in rice. <ref name="ref7" />
+Expression pattern of ''OsA8'' is investigated in rice seedlings. Transcripts of OsA8 accumulated more abundantly in roots than in leaves under normal growing condition. ''OsA8'' expression is suppressed in both roots and leaves under N, P, and K deficiency, indicating a much broader role of it in nutrient acquisition in rice. <ref name="ref7" />
 ===Mutation===
 [[File: Shijc-Os03g0100800-Fig3.jpg|right|thumb|200px|''Schematic representation of the OsA8 gene and the site of Tos17 insertion. (from reference <ref name="ref7" />).'']]
-Sequencing of the PCR products confirmed a single insertion of Tos17 in the OsA8 gene. Apart from this insertion, no other copies of Tos17 in the mutant were different from wild-type plants. Morphological comparison of the mutant and wild-type rice showed that, regardless of P supply, Osa8 mutants, in general, had smaller leaves, roots, and tillers. Consistent with these differences, biomass of both roots and shoots of the mutant plants is significantly smaller than that of wild-type plants. Notably, the biomass ratio of the root to the shoot in the mutant is significantly lower than that in the wild-type seedlings grown under P-starvation conditions. <ref name="ref7" />
+Sequencing of the PCR products confirmed a single insertion of Tos17 in the ''OsA8'' gene. Apart from this insertion, no other copies of Tos17 in the mutant were different from wild-type plants. Morphological comparison of the mutant and wild-type rice showed that, regardless of P supply, ''Osa8'' mutants, in general, had smaller leaves, roots, and tillers. Consistent with these differences, biomass of both roots and shoots of the mutant plants is significantly smaller than that of wild-type plants. Notably, the biomass ratio of the root to the shoot in the mutant is significantly lower than that in the wild-type seedlings grown under P-starvation conditions. <ref name="ref7" />
 ===Evolution===
 According to a previous classification, <ref name="ref8" /><ref name="ref9" /> OsA8 belongs to the P3A (AHA) branch, a subgroup of the P-type ATPase family that is specific to plants.
 ===Knowledge Extension===
-Among 10 OsA genes in rice, expression of OsA5, OsA9, and OsA10 is not detectable. Expression of OsA3 and OsA7 is relatively higher in both the OsA8 mutant and the wild-type seedlings. Knockout of OsA8 increased the expression of OsA1 and OsA2 in the roots of Pi-starved mutants. However, a lower abundance of OsA2 is noticed in leaves of the mutant. In addition, OsA6 appears to be induced only in roots of Pi-sufﬁcient wild-type plant. OsA4 seemed to express constitutively. This suggests a complex regulation of expression of the members of the OsA family in roots and leaves under Pi starvation conditions. It has been reported that two Pht1 genes, OsPT2 and OsPT6, were highly expressed in P-starved roots of the rice cultivar Nipponbare. <ref name="ref10" /><ref name="ref11" /> In this study, the expression of these two genes is compared in wild-type and mutant plants derived from the rice cultivar Hitomebore. The expression of OsPT2 is detected in roots and leaves of the mutant and the wild type. P-starvation did not induce the expression of OsPT6 in roots of the mutant as compared with that of the wild type. In addition, knockout of OsA8 did not noticeably affect the expression of other members of the Pht1 family. <ref name="ref7" />
+Among 10 OsA genes in rice, expression of ''OsA5'', ''OsA9'', and ''OsA10'' is not detectable. Expression of ''OsA3'' and ''OsA7'' is relatively higher in both the ''OsA8'' mutant and the wild-type seedlings. Knockout of ''OsA8'' increased the expression of ''OsA1'' and ''OsA2'' in the roots of Pi-starved mutants. However, a lower abundance of ''OsA2'' is noticed in leaves of the mutant. In addition, ''OsA6'' appears to be induced only in roots of Pi-sufﬁcient wild-type plant. ''OsA4'' seemed to express constitutively. This suggests a complex regulation of expression of the members of the OsA family in roots and leaves under Pi starvation conditions. It has been reported that two Pht1 genes, OsPT2 and OsPT6, were highly expressed in P-starved roots of the rice cultivar Nipponbare.<ref name="ref10" /><ref name="ref11" /> In this study, the expression of these two genes is compared in wild-type and mutant plants derived from the rice cultivar Hitomebore. The expression of OsPT2 is detected in roots and leaves of the mutant and the wild type. P-starvation did not induce the expression of OsPT6 in roots of the mutant as compared with that of the wild type. In addition, knockout of ''OsA8''did not noticeably affect the expression of other members of the Pht1 family. <ref name="ref7" />
 [[File: Shijc-Os03g0100800-Fig4.jpg|right|thumb|200px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]

@@ Line 19: / Line 19: @@
 ===Knowledge Extension===
 Among 10 OsA genes in rice, expression of OsA5, OsA9, and OsA10 is not detectable. Expression of OsA3 and OsA7 is relatively higher in both the OsA8 mutant and the wild-type seedlings. Knockout of OsA8 increased the expression of OsA1 and OsA2 in the roots of Pi-starved mutants. However, a lower abundance of OsA2 is noticed in leaves of the mutant. In addition, OsA6 appears to be induced only in roots of Pi-sufﬁcient wild-type plant. OsA4 seemed to express constitutively. This suggests a complex regulation of expression of the members of the OsA family in roots and leaves under Pi starvation conditions. It has been reported that two Pht1 genes, OsPT2 and OsPT6, were highly expressed in P-starved roots of the rice cultivar Nipponbare. <ref name="ref10" /><ref name="ref11" /> In this study, the expression of these two genes is compared in wild-type and mutant plants derived from the rice cultivar Hitomebore. The expression of OsPT2 is detected in roots and leaves of the mutant and the wild type. P-starvation did not induce the expression of OsPT6 in roots of the mutant as compared with that of the wild type. In addition, knockout of OsA8 did not noticeably affect the expression of other members of the Pht1 family. <ref name="ref7" />
-[[File: Shijc-Os03g0100800-Fig4.jpg|right|200px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig4.jpg|right|thumb|200px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]
-[[File: Shijc-Os03g0100800-Fig5.jpg|right|200px|''Expression of 13 phosphate transporter genes in the Pht1 family in response to phosphorus supply in the wild type and the Osa8 mutant. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig5.jpg|right|thumb|200px|''Expression of 13 phosphate transporter genes in the Pht1 family in response to phosphorus supply in the wild type and the Osa8 mutant. (from reference <ref name="ref7" />).'']]
 ==Labs working on this gene==

@@ Line 19: / Line 19: @@
 ===Knowledge Extension===
 Among 10 OsA genes in rice, expression of OsA5, OsA9, and OsA10 is not detectable. Expression of OsA3 and OsA7 is relatively higher in both the OsA8 mutant and the wild-type seedlings. Knockout of OsA8 increased the expression of OsA1 and OsA2 in the roots of Pi-starved mutants. However, a lower abundance of OsA2 is noticed in leaves of the mutant. In addition, OsA6 appears to be induced only in roots of Pi-sufﬁcient wild-type plant. OsA4 seemed to express constitutively. This suggests a complex regulation of expression of the members of the OsA family in roots and leaves under Pi starvation conditions. It has been reported that two Pht1 genes, OsPT2 and OsPT6, were highly expressed in P-starved roots of the rice cultivar Nipponbare. <ref name="ref10" /><ref name="ref11" /> In this study, the expression of these two genes is compared in wild-type and mutant plants derived from the rice cultivar Hitomebore. The expression of OsPT2 is detected in roots and leaves of the mutant and the wild type. P-starvation did not induce the expression of OsPT6 in roots of the mutant as compared with that of the wild type. In addition, knockout of OsA8 did not noticeably affect the expression of other members of the Pht1 family. <ref name="ref7" />
-[[File: Shijc-Os03g0100800-Fig4.jpg|left|thumb|200px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig4.jpg|right|200px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]
-[[File: Shijc-Os03g0100800-Fig5.jpg|left|thumb|200px|''Expression of 13 phosphate transporter genes in the Pht1 family in response to phosphorus supply in the wild type and the Osa8 mutant. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig5.jpg|right|200px|''Expression of 13 phosphate transporter genes in the Pht1 family in response to phosphorus supply in the wild type and the Osa8 mutant. (from reference <ref name="ref7" />).'']]
 ==Labs working on this gene==

@@ Line 12: / Line 12: @@
 ===Mutation===
-[[File: Shijc-Os03g0100800-Fig3.jpg|right|thumb|300px|''Schematic representation of the OsA8 gene and the site of Tos17 insertion. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig3.jpg|right|thumb|200px|''Schematic representation of the OsA8 gene and the site of Tos17 insertion. (from reference <ref name="ref7" />).'']]
 Sequencing of the PCR products confirmed a single insertion of Tos17 in the OsA8 gene. Apart from this insertion, no other copies of Tos17 in the mutant were different from wild-type plants. Morphological comparison of the mutant and wild-type rice showed that, regardless of P supply, Osa8 mutants, in general, had smaller leaves, roots, and tillers. Consistent with these differences, biomass of both roots and shoots of the mutant plants is significantly smaller than that of wild-type plants. Notably, the biomass ratio of the root to the shoot in the mutant is significantly lower than that in the wild-type seedlings grown under P-starvation conditions. <ref name="ref7" />
 ===Evolution===
@@ Line 19: / Line 19: @@
 ===Knowledge Extension===
 Among 10 OsA genes in rice, expression of OsA5, OsA9, and OsA10 is not detectable. Expression of OsA3 and OsA7 is relatively higher in both the OsA8 mutant and the wild-type seedlings. Knockout of OsA8 increased the expression of OsA1 and OsA2 in the roots of Pi-starved mutants. However, a lower abundance of OsA2 is noticed in leaves of the mutant. In addition, OsA6 appears to be induced only in roots of Pi-sufﬁcient wild-type plant. OsA4 seemed to express constitutively. This suggests a complex regulation of expression of the members of the OsA family in roots and leaves under Pi starvation conditions. It has been reported that two Pht1 genes, OsPT2 and OsPT6, were highly expressed in P-starved roots of the rice cultivar Nipponbare. <ref name="ref10" /><ref name="ref11" /> In this study, the expression of these two genes is compared in wild-type and mutant plants derived from the rice cultivar Hitomebore. The expression of OsPT2 is detected in roots and leaves of the mutant and the wild type. P-starvation did not induce the expression of OsPT6 in roots of the mutant as compared with that of the wild type. In addition, knockout of OsA8 did not noticeably affect the expression of other members of the Pht1 family. <ref name="ref7" />
-[[File: Shijc-Os03g0100800-Fig4.jpg|middle|thumb|300px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig4.jpg|left|thumb|200px|'' Expression of OsA genes in leaves and roots of the wild type and the Osa8 mutant in response to phosphorus supply. (from reference <ref name="ref7" />).'']]
-[[File: Shijc-Os03g0100800-Fig5.jpg|middle|thumb|300px|''Expression of 13 phosphate transporter genes in the Pht1 family in response to phosphorus supply in the wild type and the Osa8 mutant. (from reference <ref name="ref7" />).'']]
+[[File: Shijc-Os03g0100800-Fig5.jpg|left|thumb|200px|''Expression of 13 phosphate transporter genes in the Pht1 family in response to phosphorus supply in the wild type and the Osa8 mutant. (from reference <ref name="ref7" />).'']]
 ==Labs working on this gene==

@@ Line 14: / Line 14: @@
 ==Labs working on this gene==
-State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing
 ==References==
- Proton pump OsA8 is linked to phosphorus uptake and translocation in rice
 ==Structured Information==

@@ Line 14: / Line 14: @@
 ==Labs working on this gene==
-Please input related labs here.
 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing
-Agricultural University, Nanjing, 210095, China
+  Agricultural University, Nanjing, 210095, China
 College of Agronomy, Hainan University, Danzhou, Hainan, 571737, China.