Os03g0122600 - Revision history

Rice2012: /* Structured Information */

2015-06-12T05:00:31Z

‎Structured Information

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Fangchao at 07:12, 24 May 2014

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Fangchao at 07:11, 24 May 2014

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Fangchao at 07:10, 24 May 2014

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Fangchao at 07:07, 24 May 2014

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Fangchao at 06:59, 24 May 2014

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Fangchao at 06:53, 24 May 2014

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Fangchao at 06:41, 24 May 2014

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Fangchao at 06:36, 24 May 2014

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124.16.129.48 at 19:27, 22 July 2012

2012-07-22T19:27:22Z

@@ Line 5: / Line 5: @@
 [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference <ref name="ref1" />).'']]
-[[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|right|thumb|150px|''Schematic diagram of OsMADS50 genomic structure <ref name="ref3" />).'']]
+[[File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|right|thumb|150px|''Schematic diagram of OsMADS50 genomic structure <ref name="ref3" />).'']]
 OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions<ref name="ref3" />.

@@ Line 15: / Line 15: @@
 ===Mutation===
-[[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|left|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]
+[[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]
 OsMADS50 KO plants<ref name="ref2" />

@@ Line 5: / Line 5: @@
 [[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference <ref name="ref1" />).'']]
-[[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants <ref name="ref3" />).'']]
+[[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|right|thumb|150px|''Schematic diagram of OsMADS50 genomic structure <ref name="ref3" />).'']]
 OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions<ref name="ref3" />.

@@ Line 7: / Line 7: @@
 [[File:File:Schematic diagram of OsMADS50 genomic structure, positions of T-DNA insertional mutant alleles and flowering time of mutants.gif|150px|''Phenotype comparison between OsMADS50 KO and WT plants <ref name="ref3" />).'']]
-OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions<ref name="ref3" />.OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference <ref name="ref1" />).
+OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions<ref name="ref3" />.
 ===Mutation===
-[[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants (from reference <ref name="ref2" />).'']]
+[[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|left|thumb|200px|''Phenotype comparison between OsMADS50 KO and WT plants '']]
-OsMADS50 KO plants
+OsMADS50 KO plants<ref name="ref2" />
@@ Line 44: / Line 48: @@
 ==Labs working on this gene==
 Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)
 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University

@@ Line 8: / Line 8: @@
 OsMADS50 functions as a LD-specific flowering activator that functions only under LD conditions<ref name="ref3" />.OsMADS50 encodes MIKC-type MADS-box proteins . MADS-box genes play important roles in several developmental processes, e.g. control of flowering time, floral organ identity and fruit developmen.OsMADS50 serves as a flowering activator by controlling various floral regulators such as OsMADS1, OsMADS14, OsMADS15, OsMADS18 and Hd3a.It causing early flowering when overexpressed and delayed flowering when suppressed.OsMADS50 activates Ehd1 expression, and in turn Ehd1 activates RFT1 expression. RFT1 acts as the LD-specific florigen(from reference <ref name="ref1" />).
 ===Mutation===
 [[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants (from reference <ref name="ref2" />).'']]
 OsMADS50 KO plants
 ===Expression===
@@ Line 17: / Line 20: @@
 RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days<ref name="ref2" />.
 ===Evolution===
@@ Line 22: / Line 26: @@
 In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.<ref name="ref4" />
 ===Knowledge Extension===
@@ Line 27: / Line 32: @@
 OsMADS50 is the putative ortholog of SOC1/AGL20.
 OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that OsMADS50 acts upstream of the other MADS-box genes.
 OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.<ref name="ref3" />
 OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a<ref name="ref2" />.
 ==Labs working on this gene==
 Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)
 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University
 ==References==

@@ Line 10: / Line 10: @@
 ===Mutation===
-[[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants <ref name="ref2" />).'']]
+[[File:Phenotype comparison between OsMADS50 KO and WT plants.gif|right|thumb|150px|''Phenotype comparison between OsMADS50 KO and WT plants (from reference <ref name="ref2" />).'']]
 OsMADS50 KO plants
@@ Line 16: / Line 16: @@
 [[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference <ref name="ref2" />).'']]
-RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days.(from reference <ref name="ref2" />)
+RNA gel blot analysis revealed that OsMADS50 was variably expressed in most organs (Figure 6a). This gene was detected at a low level during the seedling stage, with transcripts increasing as the plant matured. In young panicles, expression was initially low, and continued to decline as these organs matured. RT-PCR analysis of leaves at four developmental stages confirmed the RNA gel blot analysis (Figure 6b). OsMADS50 transcript was detected at all four stages, with the expression level slightly increasing in 49-day-old plants compared with those that were 20 days old. The transcripts of Hd3a, OsMADS14, OsMADS15, and OsMADS18 increased gradually, reaching a maximum at 80 days<ref name="ref2" />.
 ===Evolution===
 [[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]
-In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.(from reference <ref name="ref4Y" />)
+In rice, OsMADS50 is the protein most homologous with SOC1/AGL20, showing 50.6% identity, whereas OsMADS56 has 48.3% identity. Genome-wide phylogenic analysis showed that OsMADS50 is clustered with the SOC1-group MADS-box proteins.<ref name="ref4" />
 ===Knowledge Extension===
-[[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56 (from reference <ref name="ref2" />).'']]
+[[File:PCE 2008 f8.gif|right|thumb|150px|'' Interaction analyses between OsMADS50 and OsMADS56<ref name="ref2" />.'']]
 OsMADS50 is the putative ortholog of SOC1/AGL20.
 OsMADS50 is upstream of OsMADS1, OsMADS14, OsMADS15, and OsMADS18.Expression analyses of the MADS-box genes in WT plants demonstrated that OsMADS50 acts upstream of the other MADS-box genes.
-OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.(from reference <ref name="ref3" />)
+OsMADS50 physically interacts with OsMADS56.Because both OsMADS50 and OsMADS56 appear to function as LD-specific regulators,the OsMADS50 KC region indeed binds to the OsMADS56 KC.<ref name="ref3" />
-OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a. (from reference <ref name="ref2" />)
+OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a<ref name="ref2" />.
 ==Labs working on this gene==
@@ Line 38: / Line 38: @@
 ==References==
 <references>
-<ref name="ref1">STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52..</ref>
+<ref name="ref1">STsuji H, Taoka K, Shimamoto K. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation[J]. Current opinion in plant biology, 2011, 14(1): 45-52.</ref>
-<ref name="ref2"Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.</ref>
+<ref name="ref2">Lee S, Kim J, Han J J, et al. Functional analyses of the flowering time gene OsMADS50, the putative SUPPRESSOR OF OVEREXPRESSION OF CO 1/AGAMOUS‐LIKE 20 (SOC1/AGL20) ortholog in rice[J]. The Plant Journal, 2004, 38(5): 754-764.</ref>
 <ref name="ref3">RYU C H, Lee S, CHO L A E H, et al. OsMADS50 and OsMADS56 function antagonistically in regulating long day (LD)‐dependent flowering in rice[J]. Plant, cell & environment, 2009, 32(10): 1412-1427.</ref>
 <ref name="ref4">Lee S Y, Jang S H, Jun S H, et al. Functional analysis of MADS-box genes expressed preferentially in vegetative tissues[C]//Advances in rice genetics, Los Baños, Laguna, Philippines, 22-27 October 2000. World Scientific Publishing Co. Pte. Ltd, 2003: 414-416.</ref>

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+OsMADS50 functions as a LD-specific flowering activator.
 ==Annotated Information==
 ===Function===
-Please input function information here.
+[[File:Molecular network.jpg|right|thumb|150px|''Molecular network (from reference <ref name="ref1" />).'']]
 ===Expression===
-Please input expression information here.
+[[File:Expression.gif|right|thumb|150px|'' Expression profiles of OsMADS50 and other flowering regulators. (from reference <ref name="ref2" />).'']]
 ===Evolution===
-Please input evolution information here.
+[[File:Dendrigram.jpg|right|thumb|150px|'Dendrigram .'']]
-You can also add sub-section(s) at will.
+OsMADS50 is epistatic to Hd3a.Three genes, OsGI, Hd1, and Hd3a, play major roles in the photoperiodic flowering pathway.The expression of OsGI and Hd1 was not affected by OsMADS50, thereby indicating that they act upstream of or in parallel with OsMADS50. In contrast, Hd3a was downregulated in the OsMADS50 KO plants, implying that OsMADS50 is epistatic to Hd3a. (from reference <ref name="ref2" />)
 ==Labs working on this gene==
-Please input related labs here.
+Department of Life Science and Functional Genomic Center, Pohang University of Science and Technology (POSTECH)
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==

← Older revision		Revision as of 19:27, 22 July 2012
Line 1:		Line 1:
		+	Please input one-sentence summary here.
		+
		+	==Annotated Information==
		+	===Function===
		+	Please input function information here.
		+
		+	===Expression===
		+	Please input expression information here.
		+
		+	===Evolution===
		+	Please input evolution information here.
		+
		+	You can also add sub-section(s) at will.
		+
		+	==Labs working on this gene==
		+	Please input related labs here.
		+
		+	==References==
		+	Please input cited references here.
		+
		+	==Structured Information==
	{{JaponicaGene\|		{{JaponicaGene\|
	GeneName = Os03g0122600\|		GeneName = Os03g0122600\|