Os03g0125100 - Revision history

Xysj1990: /* T-DNA Insertion Mutation */

2016-07-01T08:53:49Z

‎T-DNA Insertion Mutation

Xysj1990: /* Expression */

2016-07-01T08:53:27Z

‎Expression

Xysj1990: /* Expression */

2016-07-01T08:53:07Z

‎Expression

Xysj1990: /* T-DNA Insertion Mutation */

2016-07-01T08:52:48Z

‎T-DNA Insertion Mutation

Xysj1990: /* T-DNA Insertion Mutation */

2016-07-01T08:52:36Z

‎T-DNA Insertion Mutation

Xysj1990: /* Function */

2016-07-01T08:51:17Z

‎Function

Xysj1990: /* Function */

2016-07-01T08:50:45Z

‎Function

Xysj1990: /* Function */

2016-07-01T08:50:30Z

‎Function

Xysj1990: /* Knowledge Extension */

2016-07-01T08:45:55Z

‎Knowledge Extension

Xysj1990: /* Mutation */

2016-07-01T08:43:44Z

‎Mutation

@@ Line 11: / Line 11: @@
 * T-DNA insertion mutants in the background of japonica Zhonghua11 (ZH11) rice, selected from the Rice Mutant Database, for drought resistance under field conditions was screened by the researchers to identify critical genes required for drought resistance in rice. One of the drought-hypersensitive mutants showing drought sensitivity at both the seedling and panicle development stages, designated as ''dsm2-1'', was further characterized in this study.
 ====T-DNA Insertion Mutation====
-[[File:Os03g0125100-2.png|right|thumb|527px|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.'']]
+[[File:Os03g0125100-2.png|right|thumb|527px|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.''']]
 * The T-DNA insertion sites of dsm2-1 and dsm2-2 are located in the third intron and the first exon, respectively.Under normal conditions, the homozygous mutants showed no obvious phenotypic change compared with the wild-type genotype segregated from the heterozygous mutant.
 * To verify the drought-sensitive phenotype, mutant and wild-type plants at the four-leaf stage grown in sandy soil were subjected to drought stress. Under the moderate stress condition, the dsm2 mutant lines wilted faster than the wild type. After severe drought stress treatment followed by rewatering, almost all the mutant plants died, whereas wild-type plants had a significantly higher survival rate (Fig. 1C). Cosegregation analysis also suggested that the drought sensitivity was due to the T-DNA insertion in the OsBCH1 gene (data not shown). The dsm2-1 mutant was also more sensitive than the wild type to salt stress, but no significant difference was observed under cold or heat shock stress.

@@ Line 20: / Line 20: @@
 ===Expression===
-[[File:Os03g0125100-2.png|right|thumb|527px|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.'']]
+[[File:Os03g0125100-3.png|right|thumb|527px|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.''']]
 *Overexpression of ''DSM2'' in rice resulted in significantly '''increased resistance''' to '''drought''' and '''oxidative''' stresses and '''increases''' of the '''xanthophylls''' and '''nonphotochemical quenching'''. Some '''stress-related ABA responsive genes''' were '''up-regulated''' in the overexpression line<ref name="ref1"/>.

← Older revision		Revision as of 08:53, 1 July 2016
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	===Expression===		===Expression===
		+	[[File:Os03g0125100-2.png\|right\|thumb\|527px\|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.'']]
	*Overexpression of ''DSM2'' in rice resulted in significantly '''increased resistance''' to '''drought''' and '''oxidative''' stresses and '''increases''' of the '''xanthophylls''' and '''nonphotochemical quenching'''. Some '''stress-related ABA responsive genes''' were '''up-regulated''' in the overexpression line<ref name="ref1"/>.		*Overexpression of ''DSM2'' in rice resulted in significantly '''increased resistance''' to '''drought''' and '''oxidative''' stresses and '''increases''' of the '''xanthophylls''' and '''nonphotochemical quenching'''. Some '''stress-related ABA responsive genes''' were '''up-regulated''' in the overexpression line<ref name="ref1"/>.

@@ Line 11: / Line 11: @@
 * T-DNA insertion mutants in the background of japonica Zhonghua11 (ZH11) rice, selected from the Rice Mutant Database, for drought resistance under field conditions was screened by the researchers to identify critical genes required for drought resistance in rice. One of the drought-hypersensitive mutants showing drought sensitivity at both the seedling and panicle development stages, designated as ''dsm2-1'', was further characterized in this study.
 ====T-DNA Insertion Mutation====
-[[File:Os03g0125100-2.png|right|thumb|327px|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.'']]
+[[File:Os03g0125100-2.png|right|thumb|527px|'''Figure 2. Improved drought resistance of DSM2-overexpressing transgenic rice.<ref name="ref1" />.'']]
 * The T-DNA insertion sites of dsm2-1 and dsm2-2 are located in the third intron and the first exon, respectively.Under normal conditions, the homozygous mutants showed no obvious phenotypic change compared with the wild-type genotype segregated from the heterozygous mutant.
 * To verify the drought-sensitive phenotype, mutant and wild-type plants at the four-leaf stage grown in sandy soil were subjected to drought stress. Under the moderate stress condition, the dsm2 mutant lines wilted faster than the wild type. After severe drought stress treatment followed by rewatering, almost all the mutant plants died, whereas wild-type plants had a significantly higher survival rate (Fig. 1C). Cosegregation analysis also suggested that the drought sensitivity was due to the T-DNA insertion in the OsBCH1 gene (data not shown). The dsm2-1 mutant was also more sensitive than the wild type to salt stress, but no significant difference was observed under cold or heat shock stress.

@@ Line 11: / Line 11: @@
 * T-DNA insertion mutants in the background of japonica Zhonghua11 (ZH11) rice, selected from the Rice Mutant Database, for drought resistance under field conditions was screened by the researchers to identify critical genes required for drought resistance in rice. One of the drought-hypersensitive mutants showing drought sensitivity at both the seedling and panicle development stages, designated as ''dsm2-1'', was further characterized in this study.
 ====T-DNA Insertion Mutation====
 * The T-DNA insertion sites of dsm2-1 and dsm2-2 are located in the third intron and the first exon, respectively.Under normal conditions, the homozygous mutants showed no obvious phenotypic change compared with the wild-type genotype segregated from the heterozygous mutant.
 * To verify the drought-sensitive phenotype, mutant and wild-type plants at the four-leaf stage grown in sandy soil were subjected to drought stress. Under the moderate stress condition, the dsm2 mutant lines wilted faster than the wild type. After severe drought stress treatment followed by rewatering, almost all the mutant plants died, whereas wild-type plants had a significantly higher survival rate (Fig. 1C). Cosegregation analysis also suggested that the drought sensitivity was due to the T-DNA insertion in the OsBCH1 gene (data not shown). The dsm2-1 mutant was also more sensitive than the wild type to salt stress, but no significant difference was observed under cold or heat shock stress.
 * Drought sensitivity of the dsm2-1 mutant was also tested at the reproductive stage by growing the mutant and the wild type in a paddy field facilitated with a removable rain-off shelter and in polyvinyl chloride (PVC) tubes filled with sandy soil. During the course of drought stress development, dsm2-1 showed wilting earlier than the wild type. After moderate drought stress in the PVC tubes, the total grain yield of dsm2-1 was reduced by about 52% compared with the wild type, and the pollen fertility of dsm2-1 (23%) was also significantly lower than that of the wild type (58%; Fig. 1D). The root depth and volume of dsm2-1 were significantly reduced compared with the wild type (Fig. 1D), and contents of chlorophyll and Pro in dsm2-1 were also reduced prominently.
 ====Overexpression of '''''DSM2'''''====
 * To test whether DSM2 overexpression has a significant effect on improving drought resistance, the full-length cDNA of DSM2 under the control of the cauliflower mosaic virus 35S promoter (Fig. 2A) was transformed into japonica rice ZH11. After severe drought stress (no watering for 1 week), the overexpression lines had a significantly higher survival rate (approximately 74%) than the negative control (completely died; Fig. 2, C and D). After drought treatments at the reproductive stage, the overexpression lines had more green leaves and higher spikelet fertility than negative transgenic lines. The overexpression lines had less oxidative damage on the leaves and higher seed-setting rates than the negative control (Fig. 2, E and F). These results suggest that overexpression of DSM2 has a significant effect on the improvement of drought resistance in rice.

@@ Line 5: / Line 5: @@
 The rice '''''DSM2''''' gene significantly contributes to control of the xanthophyll cycle and ABA synthesis, both of which play critical roles in the establishment of drought resistance in rice <ref name="ref1"/>. The functional analysis of the '''''DSM2''''' suggested that DSM2 is essential for drought resistance in rice.
 <br><br>
-'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0003824 GO:0003824],[http://amigo.geneontology.org/amigo/term/GO:0008152 GO:0008152]
+'''GO assignment(s):''' [http://amigo.geneontology.org/amigo/term/GO:0003824 GO:0003824],[http://amigo.geneontology.org/amigo/term/GO:0008152 GO:0008152]<br><br>
-[[File:Os03g0125100-1.png|center|thumb|1000px|'''Figure 1. Identification of dsm2 T-DNA insertion mutants and its drought sensitivity.'''<ref name="ref1" />]]
+[[File:Os03g0125100-1.png|center|thumb|827px|'''Figure 1. Identification of dsm2 T-DNA insertion mutants and its drought sensitivity.'''<ref name="ref1" />]]
 ===Mutation===

@@ Line 26: / Line 26: @@
 In the rice genome, there are '''two homologs''' of ''DSM2''/ ''OsBCH1'', designated ''OsBCH2'' (LOC_Os04g48880) and ''OsBCH3'' (LOC_Os10g38940), showing '''82%''' and '''75% identity''' to ''OsBCH1'', respectively. ''DSM2'' belongs to the '''BCH family'''<ref name="ref1"/>.
-===Knowledge Extension===
+==Knowledge Extension==
 *The ''CYP97'' and ''BCH'' '''gene pairs''' are primarily responsible for hydroxylation of α- and β-carotenes, respectively, but exhibit some overlapping activities, most notably in hydroxylation of the β-ring of α-carotene. The BCH duplicates encode isozymes that show significant expression divergence in reproductive organs<ref name="ref3"/>.