Os03g0149100 - Revision history

Xysj2012: /* Structured Information */

2015-06-12T03:15:53Z

‎Structured Information

Zhangzhang: /* References */

2014-05-26T14:21:18Z

‎References

Zhangs: /* References */

2014-05-25T04:53:07Z

‎References

Zhangs: /* References */

2014-05-25T04:51:17Z

‎References

Zhangs: /* Knowledge Extension */

2014-05-25T04:50:52Z

‎Knowledge Extension

Zhangs: /* Expression Pattern */

2014-05-25T04:48:21Z

‎Expression Pattern

Zhangs: /* Mutation */

2014-05-25T04:48:10Z

‎Mutation

Zhangs: /* Function */

2014-05-25T04:47:54Z

‎Function

Zhangs: /* References */

2014-05-25T04:47:34Z

‎References

Zhangs: /* Expression Pattern */

2014-05-25T04:45:59Z

‎Expression Pattern

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 </references>
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 </ref>
 </references>
 ==Structured Information==

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 * <ref name="ref2">
 Liu H, Wang S, Yu X, et al. ARL1, a LOB‐domain protein required for adventitious root formation in rice[J]. The Plant Journal, 2005, 43(1): 47-56.
 </ref>
 * <ref name="ref4">
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 </references>
 * <ref name="ref3">
-Juan Zhang, Youliang Peng, Zejian Guo.Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants,Cell Research (2008) 18:508-521.
+Juan Zhang, Youliang Peng, Zejian Guo.Constitutive expression of pathogen-inducible OsWRKY31 enhances disease resistance and affects root growth and auxin response in transgenic rice plants,Cell Research ,2008, 18:508-521.
 </ref>

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 Liu H, Wang S, Yu X, et al. ARL1, a LOB‐domain protein required for adventitious root formation in rice[J]. The Plant Journal, 2005, 43(1): 47-56.
 </ref>
-* <ref name="ref3">
+* <ref name="ref4">
 Husbands A, Bell E M, Shuai B, et al. LATERAL ORGAN BOUNDARIES defines a new family of DNA-binding transcription factors and can interact with specific bHLH proteins[J]. Nucleic Acids Research, 2007, 35(19): 6663-6671.
 </ref>
-* <ref name="ref4">
+* <ref name="ref5">
 Shuai B, Reynaga-Peña C G, Springer P S. The lateral organ boundaries gene defines a novel, plant-specific gene family[J]. Plant physiology, 2002, 129(2): 747-761.
 </ref>
-* <ref name="ref5">
+* <ref name="ref6">
 Mangeon A, Bell E M, Lin W, et al. Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis[J]. Journal of experimental botany, 2011, 62(1): 221-233.
 </ref>

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 ===Knowledge Extension===
-* The ''LATERAL ORGAN BOUNDARIES DOMAIN '''(LBD)''' '' genes define a large, plant-specific family of largely unknown function<ref name="ref3" /><ref name="ref4" /><ref name="ref5" />. The LOB protein encodes a conserved approximately 100-amino acid domain which contains a motif resembling a zinc finger and another similar to a leucine zipper<ref name="ref3" /><ref name="ref4" />. However, both motifs have atypical spacing and until now, the biochemical function of the LOB domain has not been defined, although proteins containing LOB domains have been assumed to be transcription factors<ref name="ref3" />.The discovery of '''''OsCrl1''''' implies that some members of the LOB domain gene family may play an important role in organ development in plants<ref name="ref2" />.
+* The ''LATERAL ORGAN BOUNDARIES DOMAIN '''(LBD)''' '' genes define a large, plant-specific family of largely unknown function<ref name="ref4" /><ref name="ref5" /><ref name="ref6" />. The LOB protein encodes a conserved approximately 100-amino acid domain which contains a motif resembling a zinc finger and another similar to a leucine zipper<ref name="ref4" /><ref name="ref5" />. However, both motifs have atypical spacing and until now, the biochemical function of the LOB domain has not been defined, although proteins containing LOB domains have been assumed to be transcription factors<ref name="ref4" />.The discovery of '''''OsCrl1''''' implies that some members of the LOB domain gene family may play an important role in organ development in plants<ref name="ref2" />.
-* In Arabidopsis, LOB defines a subgroup of LBD genes that also includes ''LBD10/ASL2, LBD25/ASL3, LBD36/ASL1, and AS2/LBD6''<ref name="ref4" /><ref name="ref5" />. Among this subgroup, '''''AS2''' (ASYMMETRIC LEAVES2)'' is the only gene with clearly defined functions. '''''AS2''''' is required to prevent expression of the class I ''KNOX'' homeobox genes ''BREVIPEDICELLUS ('''BP''')'', ''KNAT2'', and ''KNAT6'' in the leaf. '''''AS2''''' is expressed on the adaxial side of lateral organs and misexpression leads to the formation of adaxialized leaves implicating '''''AS2''''' in adaxial cell fate specification<ref name="ref5" />.
+* In Arabidopsis, LOB defines a subgroup of LBD genes that also includes ''LBD10/ASL2, LBD25/ASL3, LBD36/ASL1, and AS2/LBD6''<ref name="ref5" /><ref name="ref6" />. Among this subgroup, '''''AS2''' (ASYMMETRIC LEAVES2)'' is the only gene with clearly defined functions. '''''AS2''''' is required to prevent expression of the class I ''KNOX'' homeobox genes ''BREVIPEDICELLUS ('''BP''')'', ''KNAT2'', and ''KNAT6'' in the leaf. '''''AS2''''' is expressed on the adaxial side of lateral organs and misexpression leads to the formation of adaxialized leaves implicating '''''AS2''''' in adaxial cell fate specification<ref name="ref6" />.
 ==Labs working on this gene==

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 * The expression of '''''OsCrl1''''' was induced within 1 h and increased dramatically until 3 h after IAA treatment, then it gradually decreased. This suggests that '''''OsCrl1''''' is a member of the early auxin response family<ref name="ref1" />.
 [[File:Crl_3.jpg|right|thumb|300px|'''Figure 3.''' ''An unrooted dendogram of the AS2/LOB family proteins in Arabidopsis, maize and rice(from reference) <ref name="ref1" />.'']]
-The primers used were 5´-agc aac gtg tcc aag ctg ct-3´ and 5´-tgt agc cgc cgt acc cta at-3´ for OsCrl1 (AB200234).<ref name="ref6" />
+The primers used were 5´-agc aac gtg tcc aag ctg ct-3´ and 5´-tgt agc cgc cgt acc cta at-3´ for OsCrl1 (AB200234).<ref name="ref3" />
 ===Subcellular localization===

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 * Adventitious root primordia were induced at the fourth node of 8-week-old wild-type plants by submergence for 24 h (Figure 1e), but were not induced in the '''''OsArl1''''' mutant (Figure 2f).
 Interestingly,constitutive expression of the OsIAA4 and OsCrl1 genes was observed in the OsWRKY31 overexpressor lines of T2 progenies and their expressions were not increased further by the treatment of the overexpressor plants with IBA (Figure 8C). These results suggest that OsWRKY31 may be involved in auxin signaling.
-We also found that the induction of OsWRKY31 was even faster than the expression of auxin-inducible OsIAA4 and OsCrl1 genes following treatment of rice seedlings with IBA (Figure 8B).The early auxin response of OsWRKY31 expression suggests that OsWRKY31 is a member of the early auxin response family. However, constitutive expression of auxin-inducible OsIAA4 and OsCrl1 genes in OsWRKY31 overexpressor lines did not exhibit corresponding phenotypes,such as more or longer lateral roots (Figure 8)<ref name="ref6" />.
+We also found that the induction of OsWRKY31 was even faster than the expression of auxin-inducible OsIAA4 and OsCrl1 genes following treatment of rice seedlings with IBA (Figure 8B).The early auxin response of OsWRKY31 expression suggests that OsWRKY31 is a member of the early auxin response family. However, constitutive expression of auxin-inducible OsIAA4 and OsCrl1 genes in OsWRKY31 overexpressor lines did not exhibit corresponding phenotypes,such as more or longer lateral roots (Figure 8)<ref name="ref3" />.
-An explanation for this dissimilarity may be that the constitutive expression in some of the rice Aux/IAA genes,such as OsIAA4, might lead to the stabilization of these proteins at some specific stage or condition of development.In support of this idea, some SLR1/IAA14 overexpressing Arabidopsis plants exhibit an slr (solitary-root)-1-like phenotype, which was interpreted by Fukaki et al. to be the accumulation of IAA14 proteins in these 35S::IAA14 plants at the CaMV35S promoter, which is constitutively active in most plant tissues. Furthermore, even though rice Crl1 is a positive regulator of crown and lateral root formation, its expression has been found to be insufficient to initiate crown or lateral root development.<ref name="ref6" />
+An explanation for this dissimilarity may be that the constitutive expression in some of the rice Aux/IAA genes,such as OsIAA4, might lead to the stabilization of these proteins at some specific stage or condition of development.In support of this idea, some SLR1/IAA14 overexpressing Arabidopsis plants exhibit an slr (solitary-root)-1-like phenotype, which was interpreted by Fukaki et al. to be the accumulation of IAA14 proteins in these 35S::IAA14 plants at the CaMV35S promoter, which is constitutively active in most plant tissues. Furthermore, even though rice Crl1 is a positive regulator of crown and lateral root formation, its expression has been found to be insufficient to initiate crown or lateral root development.<ref name="ref3" />
 ===Expression Pattern===

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 ===Function===
 '''''OsCrl1''''' encodes an ASYMMETRIC LEAVES2 (AS2) / LATERAL ORGAN BOUNDARIES (LOB) domain transcription factor which acts as a positive regulator for crown and lateral root formation in rice <ref name="ref1" /><ref name="ref2" />. As an auxin-responsive factor involved in auxin-mediated cell dedifferentiation, '''''OsCrl1''''' can promote the initial cell division in the pericycle cells adjacent to the peripheral vascular cylinder in the stem<ref name="ref2" />.Manipulation of the regulation of this gene (and its homologs in other crops) has great potential for increasing the ability of crops to absorb water and nutrients from the soil through improvement in their root architecture, which is required for achieving high yields<ref name="ref2" />.
-OsCrl1 genes is well known as early auxin-response genes,an essential gene for crown root formation in rice.<ref name="ref6" />
+OsCrl1 genes is well known as early auxin-response genes,an essential gene for crown root formation in rice.<ref name="ref3" />
+.