Os03g0168600 - Revision history

Rice2012: /* Structured Information */

2015-06-12T05:03:45Z

‎Structured Information

Tuly: /* Evolution */

2014-06-07T12:43:56Z

‎Evolution

Tuly: /* Evolution */

2014-06-05T13:13:01Z

‎Evolution

Tuly: /* Mutation */

2014-06-05T13:10:29Z

‎Mutation

Tuly: /* Evolution */

2014-06-05T13:09:58Z

‎Evolution

Tuly: /* Evolution */

2014-06-05T13:09:18Z

‎Evolution

Tuly: /* Mutation */

2014-06-05T13:08:17Z

‎Mutation

Tuly: /* Labs working on this gene */

2014-06-05T13:03:24Z

‎Labs working on this gene

Tuly: /* References */

2014-06-05T12:57:26Z

‎References

Tuly: /* Expression */

2014-06-05T12:52:42Z

‎Expression

@@ Line 57: / Line 57: @@
 To verify the gene structure of the CYP704B2 gene, we determined exon-intron junctions by sequencing the cDNA frag- ment ampliﬁed by RT-PCR. Comparison with the genomic sequence indicated thatCYP704B2 contained four exons and three introns (Figure 6B). CYP704B2 putatively encodes a cytochrome P450 protein with 545 amino acids containing a transmembrane segment of 23 amino acids (VTSFFPVAGIHKLIAIFLVVLSW) in the N-terminal region (Figure 6C).[[File:huangfengjie6.jpg]]
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 31: / Line 31: @@
 ===Evolution===
-Each plant species has a large number of P450 members with a diversity of biological functions in biosynthetic and catabolic pathways[2]. A total of 39 putative ESTs and annotated protein sequences that are related to riceCYP704B2 were obtained from 19 different species. Along with the six and two members of the CYP704A subfamily encoded by the genome of rice and Arabidopsis, respectively (Figure 7), these P450s belonged to four families all from the CYP86 clan: CYP86, CYP94, CYP704, and CYP96.[[File:huangfengjie5.jpg]][[File:huangfengjie7]]
+Each plant species has a large number of P450 members with a diversity of biological functions in biosynthetic and catabolic pathways[2]. A total of 39 putative ESTs and annotated protein sequences that are related to riceCYP704B2 were obtained from 19 different species. Along with the six and two members of the CYP704A subfamily encoded by the genome of rice and Arabidopsis, respectively (Figure 7), these P450s belonged to four families all from the CYP86 clan: CYP86, CYP94, CYP704, and CYP96.[[File:huangfengjie5.jpg]]
 ==Labs working on this gene==

@@ Line 31: / Line 31: @@
 ===Evolution===
-Each plant species has a large number of P450 members with a diversity of biological functions in biosynthetic and catabolic pathways[2]. A total of 39 putative ESTs and annotated protein sequences that are related to riceCYP704B2 were obtained from 19 different species. Along with the six and two members of the CYP704A subfamily encoded by the genome of rice and Arabidopsis, respectively (Figure 7), these P450s belonged to four families all from the CYP86 clan: CYP86, CYP94, CYP704, and CYP96.[[File:huangfengjie5.jpg]]
+Each plant species has a large number of P450 members with a diversity of biological functions in biosynthetic and catabolic pathways[2]. A total of 39 putative ESTs and annotated protein sequences that are related to riceCYP704B2 were obtained from 19 different species. Along with the six and two members of the CYP704A subfamily encoded by the genome of rice and Arabidopsis, respectively (Figure 7), these P450s belonged to four families all from the CYP86 clan: CYP86, CYP94, CYP704, and CYP96.[[File:huangfengjie5.jpg]][[File:huangfengjie7]]
 ==Labs working on this gene==

@@ Line 28: / Line 28: @@
 outermost linear-shaped surface of the wild-type anther (Figure 4C), and the pollen developed the exquisite exine pattern and a visible germination pore
 (Figure 4D). However, a glossy and smooth anther surface (Figure 4E) and shrunken microspores (Figure 4F) in cyp704B2 were observed as a result of the
-undeveloped cuticle/epicuticular structures and lack of obvious pollen exine.[[File:huangfengjie4.jpg]][[File:huangfengjie6.jpg]]
+undeveloped cuticle/epicuticular structures and lack of obvious pollen exine.[[File:huangfengjie4.jpg]]
 ===Evolution===

@@ Line 31: / Line 31: @@
 ===Evolution===
-Each plant species has a large number of P450 members with a diversity of biological functions in biosynthetic and catabolic pathways[2]. A total of 39 putative ESTs and annotated protein sequences that are related to riceCYP704B2 were obtained from 19 different species. Along with the six and two members of the CYP704A subfamily encoded by the genome of rice and Arabidopsis, respectively (Figure 7), these P450s belonged to four families all from the CYP86 clan: CYP86, CYP94, CYP704, and CYP96.[[File:huangfengjie5.jpg]][[File:huangfengjie6.jpg]]
+Each plant species has a large number of P450 members with a diversity of biological functions in biosynthetic and catabolic pathways[2]. A total of 39 putative ESTs and annotated protein sequences that are related to riceCYP704B2 were obtained from 19 different species. Along with the six and two members of the CYP704A subfamily encoded by the genome of rice and Arabidopsis, respectively (Figure 7), these P450s belonged to four families all from the CYP86 clan: CYP86, CYP94, CYP704, and CYP96.[[File:huangfengjie5.jpg]]
 ==Labs working on this gene==

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 ==Labs working on this gene==
-Please input related labs here.
+School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, China
 ==References==

@@ Line 38: / Line 38: @@
 ==References==
 [1] Hui Li, Franck Pinot, Vincent Sauveplane, Danie ` le Werck-Reichhart, Patrik Diehl, Lukas Schreiber, Rochus Franke, Ping Zhang, Liang Chen, Yawei Gao, Wanqi Liang,and Dabing Zhang(2010).Cytochrome P450 Family Member CYP704B2 Catalyzes the w-Hydroxylation of Fatty Acids and Is Required for Anther Cutin Biosynthesis and Pollen Exine Formation in Rice. The Plant Cell, Vol. 22: 173–190.
 [2] Schuler, M.A., and Werck-Reichhart, D. (2003). Functional genomics of P450s. Annu. Rev. Plant Biol. 54: 629–667.
 ==Structured Information==

@@ Line 13: / Line 13: @@
 ===Expression===
 The CYP704B2 transcript is specifically detected in the tapetum and the microspore from stage 8 of anther development to stage 10. Heterologous expression of CYP704B2 in yeast demonstrated that CYP704B2 catalyzes the production of v-hydroxylated fatty acids with 16 and 18 carbon chains.
-CYP704B2was predicted to be expressed in immature rice panicles. In addition, recent expression proﬁle data from laser microdissection mediated microarray analysis showed that the CYP704B2 gene was expressed in both the tapetum and microspores in the developing anther[3]. Consistently,  that CYP704B2 was expressed in the anther from stage 8 to stage 10, but not in the root, stem, leaf palea/lemma, and pistil (Figure 8A). CYP704B2 expression started at stage 8, reached the highest level at stage 10, and then dropped to an undetectable level at stage 13 (Figure 8A). In transgenic plants expressing the CYP704B2pro:GUS fusion (for expression of the -glucuronidase [GUS] marker protein driven by the promoter region of CYP704B2), GUS staining was speciﬁcally detected in the anther from stage 8 to stage 10, and no expression was found in the palea, lemma, and pistil (Figures 8B and 8C). When observed by phase-contrast microscopy using cleared mounted anthers (Willemsen et al., 1998), only the tapetal layer and microspores showed GUS staining (Figures 8D and 8E). All data therefore converge to indicate a speciﬁc function of CYP704B2 in the tapetal cells and microspores essential for anther and pollen development.
+CYP704B2was predicted to be expressed in immature rice panicles. In addition, recent expression proﬁle data from laser microdissection mediated microarray analysis showed that the CYP704B2 gene was expressed in both the tapetum and microspores in the developing anther[3]. Consistently,  that CYP704B2 was expressed in the anther from stage 8 to stage 10, but not in the root, stem, leaf palea/lemma, and pistil (Figure 8A). CYP704B2 expression started at stage 8, reached the highest level at stage 10, and then dropped to an undetectable level at stage 13 (Figure 8A). In transgenic plants expressing the CYP704B2pro:GUS fusion (for expression of the -glucuronidase [GUS] marker protein driven by the promoter region of CYP704B2), GUS staining was speciﬁcally detected in the anther from stage 8 to stage 10, and no expression was found in the palea, lemma, and pistil (Figures 8B and 8C). When observed by phase-contrast microscopy using cleared mounted anthers[4], only the tapetal layer and microspores showed GUS staining (Figures 8D and 8E). All data therefore converge to indicate a speciﬁc function of CYP704B2 in the tapetal cells and microspores essential for anther and pollen development.
 [[File:huangfengjie1.jpg]]