Os03g0203200 - Revision history

Zhennan at 07:24, 29 June 2016

2016-06-29T07:24:17Z

Zhennan at 07:55, 25 June 2016

2016-06-25T07:55:31Z

Rice2012: /* Structured Information */

2015-06-12T05:06:34Z

‎Structured Information

Liuhechun: /* Annotated Information */

2014-06-09T03:21:03Z

‎Annotated Information

Liuhechun: /* 基因信息 */

2014-06-09T03:18:29Z

‎基因信息

Liuhechun: /* Structured Information */

2014-06-09T03:17:03Z

‎Structured Information

Liuhechun: /* Function */

2014-06-09T03:15:30Z

‎Function

Liuhechun: /* References */

2014-06-09T03:13:02Z

‎References

Liuhechun: /* Structured Information */

2014-06-09T03:11:43Z

‎Structured Information

Liuhechun: /* References */

2014-06-09T03:05:35Z

‎References

← Older revision		Revision as of 07:24, 29 June 2016
Line 1:		Line 1:
−	~~Please input one-sentence summary here.~~

−	~~HTD2gene could negatively regulate tiller bud outgrowth by the strigolactone pathway~~
	HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway		HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway
		+

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 07:55, 25 June 2016
Line 14:		Line 14:

	The results suggest that the HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway <ref name="ref3" />. which is characterized by a high tillering and dwarf phenotype. Phenotypic analysis of the mutant showed that the mutation did not aVect formation of tiller bud, but promoted the subsequent outgrowth of tiller bud <ref name="ref3" />.		The results suggest that the HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway <ref name="ref3" />. which is characterized by a high tillering and dwarf phenotype. Phenotypic analysis of the mutant showed that the mutation did not aVect formation of tiller bud, but promoted the subsequent outgrowth of tiller bud <ref name="ref3" />.
−	~~====基因信息====~~
−	HTD2，亦称D88 或D14，编码一个酯酶，抑制水稻分枝发生，负调节水稻分蘖数。D14 可能是独角金内酯（Strigolactones, SLs）信号途径的一个组分，在其下游起作用。中国的2个小组和日本的1个小组在同一年报道了对该基因的克隆。
−	分蘖数由内源和外源信号分子所调节的分蘖芽的形成及随后向外生长所决定。htd2 是从15000个转基因株系中分离出的一个水稻突变体，该突变体分蘖数增多且矮化。表型分析表明HTD2 的突变不影响分蘖芽的形成，但促进了随后分蘖芽的向外生长。通过图位克隆的方法并设计17个新的插入/缺失分子标记来分离HTD2 基因，利用F2 和F3 群体构建了HTD2 基因附近的高分辨率物理图谱，该基因位于BAC克隆OSJNBa0009J13上，最终定位在相距12.8kb的DNA标记HT41和HT52之间。对突变体12.8kb的目标区域进行克隆和测序发现T-DNA的插入导致一个酯酶/脂肪酶/硫酯酶家族基因的启动子和第一个外显子之间相距463bp的片段缺失。另外，该基因表达受抑制的转基因植株出现分蘖增多和矮化的表型。因此，这个酯酶/脂肪酶/硫酯酶家族基因即为HTD2 基因（Liu et al., 2009）。
−	~~Link = [http://www.ricedata.cn/gene/list/956.htm]\|~~
−
−	~~====基因克隆与生物学功能分析====~~
−	HTD2 cDNA全长1487bp，包含有2 个外显子，编码一个由318 氨基酸组成的蛋白产物。T-DNA的插入导致HTD2 的启动子和第一个外显子之间相距463bp的片段缺失（Liu et al., 2009）

	===Expression===		===Expression===

@@ Line 63: / Line 63: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Oryza Sativa Japonica Group]]

@@ Line 5: / Line 5: @@
 ==Annotated Information==
 ===Function===
 The function of Dwarf 88 was confirmed by complementation test and antisense analysis. D88, thus, represents a new category of genes that regulates cell growth and organ development and consequently plant architecture. The potential relationship between the tiller formation associated genes and D88 is discussed and future identification of the substrate for D88 may lead to the characterization of new pathways regulating plant development <ref name="ref1" />.

@@ Line 14: / Line 14: @@
 HTD2，亦称D88 或D14，编码一个酯酶，抑制水稻分枝发生，负调节水稻分蘖数。D14 可能是独角金内酯（Strigolactones, SLs）信号途径的一个组分，在其下游起作用。中国的2个小组和日本的1个小组在同一年报道了对该基因的克隆。
 分蘖数由内源和外源信号分子所调节的分蘖芽的形成及随后向外生长所决定。htd2 是从15000个转基因株系中分离出的一个水稻突变体，该突变体分蘖数增多且矮化。表型分析表明HTD2 的突变不影响分蘖芽的形成，但促进了随后分蘖芽的向外生长。通过图位克隆的方法并设计17个新的插入/缺失分子标记来分离HTD2 基因，利用F2 和F3 群体构建了HTD2 基因附近的高分辨率物理图谱，该基因位于BAC克隆OSJNBa0009J13上，最终定位在相距12.8kb的DNA标记HT41和HT52之间。对突变体12.8kb的目标区域进行克隆和测序发现T-DNA的插入导致一个酯酶/脂肪酶/硫酯酶家族基因的启动子和第一个外显子之间相距463bp的片段缺失。另外，该基因表达受抑制的转基因植株出现分蘖增多和矮化的表型。因此，这个酯酶/脂肪酶/硫酯酶家族基因即为HTD2 基因（Liu et al., 2009）。
 ====基因克隆与生物学功能分析====
 HTD2 cDNA全长1487bp，包含有2 个外显子，编码一个由318 氨基酸组成的蛋白产物。T-DNA的插入导致HTD2 的启动子和第一个外显子之间相距463bp的片段缺失（Liu et al., 2009）

@@ Line 108: / Line 108: @@
        ctattggaaa aaaaattaca ttactggagt gcttcgtgca tacaatt</dnaseqindica>|
 Link = [http://www.ncbi.nlm.nih.gov/nuccore/32980850]|
 }}
 [[Category:Genes]]

← Older revision		Revision as of 03:15, 9 June 2014
Line 11:		Line 11:

	The results suggest that the HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway <ref name="ref3" />. which is characterized by a high tillering and dwarf phenotype. Phenotypic analysis of the mutant showed that the mutation did not aVect formation of tiller bud, but promoted the subsequent outgrowth of tiller bud <ref name="ref3" />.		The results suggest that the HTD2 gene could negatively regulate tiller bud outgrowth by the strigolactone pathway <ref name="ref3" />. which is characterized by a high tillering and dwarf phenotype. Phenotypic analysis of the mutant showed that the mutation did not aVect formation of tiller bud, but promoted the subsequent outgrowth of tiller bud <ref name="ref3" />.
		+	====基因信息====
		+	HTD2，亦称D88 或D14，编码一个酯酶，抑制水稻分枝发生，负调节水稻分蘖数。D14 可能是独角金内酯（Strigolactones, SLs）信号途径的一个组分，在其下游起作用。中国的2个小组和日本的1个小组在同一年报道了对该基因的克隆。
		+	分蘖数由内源和外源信号分子所调节的分蘖芽的形成及随后向外生长所决定。htd2 是从15000个转基因株系中分离出的一个水稻突变体，该突变体分蘖数增多且矮化。表型分析表明HTD2 的突变不影响分蘖芽的形成，但促进了随后分蘖芽的向外生长。通过图位克隆的方法并设计17个新的插入/缺失分子标记来分离HTD2 基因，利用F2 和F3 群体构建了HTD2 基因附近的高分辨率物理图谱，该基因位于BAC克隆OSJNBa0009J13上，最终定位在相距12.8kb的DNA标记HT41和HT52之间。对突变体12.8kb的目标区域进行克隆和测序发现T-DNA的插入导致一个酯酶/脂肪酶/硫酯酶家族基因的启动子和第一个外显子之间相距463bp的片段缺失。另外，该基因表达受抑制的转基因植株出现分蘖增多和矮化的表型。因此，这个酯酶/脂肪酶/硫酯酶家族基因即为HTD2 基因（Liu et al., 2009）。
		+	====基因克隆与生物学功能分析====
		+	HTD2 cDNA全长1487bp，包含有2 个外显子，编码一个由318 氨基酸组成的蛋白产物。T-DNA的插入导致HTD2 的启动子和第一个外显子之间相距463bp的片段缺失（Liu et al., 2009）

	===Expression===		===Expression===

@@ Line 51: / Line 51: @@
 <ref name="NATURE rice-1">Guo S, Xu Y, Liu H, et al. The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14[J]. Nature communications 2013; 4: 1566.</ref>
 </references>
 ==Structured Information==
 {{JaponicaGene|

@@ Line 65: / Line 65: @@
              clade; Ehrhartoideae; Oryzeae; Oryza.
+|
 Chromosome = [[:category:Japonica Chromosome 2|Chromosome 2]]|
 AP = Chromosome 3:203..1159|

← Older revision		Revision as of 03:05, 9 June 2014
Line 51:		Line 51:
	<ref name="NATURE rice-1">Guo S, Xu Y, Liu H, et al. The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14[J]. Nature communications 2013; 4: 1566.</ref>		<ref name="NATURE rice-1">Guo S, Xu Y, Liu H, et al. The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14[J]. Nature communications 2013; 4: 1566.</ref>
	</references>		</references>
		+	==Structured Information==
		+	{{JaponicaGene\|
		+	GeneName = AK070827\|
		+	Description = 1487 bp mRNA linear PLN 04-DEC-2008.\|
		+	Version = AK070827.1 GI:32980850\|
		+	Length =1487 bp bp\|
		+	Definition = Oryza sativa Japonica Group cDNA clone:J023064N24, full insert
		+	sequence.\|
		+	Source = Oryza sativa Japonica Group (Japanese rice)
		+	ORGANISM Oryza sativa Japonica Group
		+	Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
		+	Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
		+	clade; Ehrhartoideae; Oryzeae; Oryza.
		+	\|
		+	Chromosome = [[:category:Japonica Chromosome 2\|Chromosome 2]]\|
		+	AP = Chromosome 3:203..1159\|
		+	CDS =203..1159\|
		+
		+	AA = <aaseq>MLRSTHPPPSSPSSSSSGGGGGGGSSASSSSEKTMVGGGGGGGG
		+	GSGSAAPSGAKLLQILNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYD
		+	LVCAGSVNPDHFDFRRYDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRR
		+	PDLFAKLVLIGASPRFLNDSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADV
		+	PAAVQEFSRTLFNMRPDISLHVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVA
		+	AYLKAHLGGRTTVEFLQTEGHLPHLSAPSLLAQVLRRALARY</aaseq>\|
		+	DNA =<dnaseqindica>1..61#121..181#241..301#361..421# 481..541#601..661#721..781#841..901#961..1021#1081..1141#1201..1261#1321..1381#1441# gacaaaaatc ccaatctacc accgcaccca acgaagctta agacagtgga ataaagcaga
		+	gagagaaatc aaagcaagaa agagagagaa gaagcgaggc aagagtcaag accatctccc
		+	atggccagtg gtgatagtag tagtagtagt agtatataaa aggggagcgc gccacggctt
		+	gccaatccgc cgcgctggtg tgatgctgcg atcgacgcat ccgccgccca gtagcccgag
		+	cagcagcagc agcggcggcg gcgggggcgg ggggtcgtcg gcgtcgtcga gctcggagaa
		+	gacgatggtg ggcggcgggg gaggaggggg aggagggagc gggtcggcgg cgccgagcgg
		+	ggcgaagctg ctgcagatcc tgaacgtgcg ggtggtgggg agcggcgagc gggtggtggt
		+	gctgtcgcat ggcttcggga cggaccagtc ggcgtggagc cgcgtgctgc cgtacctcac
		+	ccgcgaccac cgcgtcgtgc tctacgacct cgtctgcgcc ggcagcgtca acccggacca
		+	cttcgacttc cgccgctacg acaacctcga cgcctacgtc gacgacctgc tcgccatcct
		+	cgacgcgctc cgcatcccgc gctgcgcctt cgtcggccac tccgtctccg ccatgatcgg
		+	catcctcgcc tccatccgac gacctgacct cttcgccaag cttgtcctca tcggcgcctc
		+	tccccggttc ttgaacgaca gcgactacca cggcgggttc gagctggagg agatacagca
		+	ggtgttcgac gcgatggggg cgaactactc ggcgtgggcg acggggtacg cgcctctggc
		+	ggtgggcgcc gacgtgccgg cggcggtgca ggagttcagc cgcaccctct tcaacatgcg
		+	cccggacatc tccctccacg tctgccagac cgtcttcaag accgacctcc gcggcgtgct
		+	cggcatggtc cgcgccccct gcgtcgtcgt ccagaccacc cgcgacgtct ccgtcccggc
		+	ctccgtcgcc gcctacctca aggcccacct cggcggccgc accaccgtcg agttcctcca
		+	gaccgagggt cacctccccc acctcagcgc ccccagcctc ctcgcccagg tgctccgccg
		+	cgctctcgcc cggtactaat ccaacgcccc ccgcacgcca ccgcggcgcg ccgcgcgtgc
		+	gcgtagccgc agcgacgagt caaaacgaag gagaaaaaaa aaaaccaccg cagctgcggc
		+	tacggtacgt gtggcccagt agggggacac gcatccctct actgtttttt ttttttttac
		+	cgcgtcggat tttactgcat ttttaccctc ctcttctcag gcctagaaaa tatttcggcg
		+	cgacctgcca agaccgagag tgtactgtag cattagtgct cgatttttac ctaccttact
		+	ctattggaaa aaaaattaca ttactggagt gcttcgtgca tacaatt</dnaseqindica>\|
		+	Link = [http://www.ncbi.nlm.nih.gov/nuccore/32980850]\|
		+	}}
		+	[[Category:Genes]]
		+	[[Category:Oryza Sativa Japonica Group]]
		+	[[Category:Japonica Genes]]
		+	[[Category:Japonica Chromosome 3]]
		+	[[Category:Chromosome 3]]