Os03g0216700 - Revision history

Liman at 07:53, 30 July 2016

2016-07-30T07:53:27Z

2016-07-30T07:47:51Z

‎References

2016-07-30T07:47:33Z

‎Mutation

2016-07-30T07:43:15Z

2016-07-30T07:42:08Z

2016-07-30T07:40:05Z

‎Function

2016-07-30T07:32:26Z

‎References

2016-07-30T07:31:27Z

2016-07-30T07:26:13Z

‎Expression Pattern

2016-07-30T07:23:33Z

‎Mutation

← Older revision		Revision as of 07:53, 30 July 2016
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−	~~Please input one-sentence summary here~~.	+	The rice gene Os03g0216700 was reported as OsFRDL1 in 2004<ref name="ref2" />
−		+	.
	==Annotated Information==		==Annotated Information==
	===Function===		===Function===

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 Inoue H, Mizuno D, Takahashi M, Nakanishi H, Mori S, Nishizawa NK (2004) A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport. Soil Sci Plant Nutr 50: 1133–1140
 </ref>
 </references>
 ==Structured Information==
       [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

← Older revision		Revision as of 07:47, 30 July 2016
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	* OsFRDL1 is not responsible for Al-induced secretion of citrate from the rice roots<ref name="ref1" />.		* OsFRDL1 is not responsible for Al-induced secretion of citrate from the rice roots<ref name="ref1" />.
		+
		+	===Evolution===
		+	* OsFRDL1 (Os03g0216700) was initially isolated as a homolog of AtFRD3 (Fig. 2)<ref name="ref1" />. In the rice genome, there are six close homolog genes of AtFRD3, HvAACT1, and SbMATE (Fig. 2)
		+
		+	[[File:Os03g0216700-7.png\|center\|thumb\|400px\|'''Figure 2. Phylogenetic relationship of OsFRDL1-like proteins in different plant species. The amino acid sequences were aligned by ClustalW.''' ''<ref name="ref1" />.'']]

	===Mutation===		===Mutation===

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-* OsFRDL1 encodes a transporter for citrate at the plasma membrane of pericycle cells, which is required for efficient translocation of Fe3+ as a citrate complex from the roots to the shoots. To confirm whether OsFRDL1 has activity to transport citrate, the researchers expressed OsFRDL1 in Xenopus oocytes. The efflux activity for citrate was significantly higher in the oocytes injected with OsFRDL1 cRNA than in oocytes injected with water (Fig. 1). This result indicates that OsFRDL1 is able to transport citrate out of the cells.<ref name="ref1" />.
+* The results suggested that OsFRDL1 encodes a transporter for citrate at the plasma membrane of pericycle cells, which is required for efficient translocation of Fe3+ as a citrate complex from the roots to the shoots. To confirm whether OsFRDL1 has activity to transport citrate, the researchers expressed OsFRDL1 in Xenopus oocytes. The efflux activity for citrate was significantly higher in the oocytes injected with OsFRDL1 cRNA than in oocytes injected with water (Fig. 1). This result indicates that OsFRDL1 is able to transport citrate out of the cells.<ref name="ref1" />.
 [[File:Os03g0216700-6.png|center|thumb|400px|'''Figure 1. Efflux transport activity for citrate in Xenopus oocytes.

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-* OsFRDL1 encodes a transporter for citrate at the plasma membrane of pericycle cells, which is required for efficient translocation of Fe3+ as a citrate complex from the roots to the shoots. To confirm whether OsFRDL1 has activity to
+* OsFRDL1 encodes a transporter for citrate at the plasma membrane of pericycle cells, which is required for efficient translocation of Fe3+ as a citrate complex from the roots to the shoots. To confirm whether OsFRDL1 has activity to transport citrate, the researchers expressed OsFRDL1 in Xenopus oocytes. The efflux activity for citrate was significantly higher in the oocytes injected with OsFRDL1 cRNA than in oocytes injected with water (Fig. 1). This result indicates that OsFRDL1 is able to transport citrate out of the cells.<ref name="ref1" />.
 * OsFRDL1 is not responsible for Al-induced secretion of citrate from the rice roots<ref name="ref1" />.

@@ Line 20: / Line 20: @@
 ===Expression Pattern===
 * The expression of OsFRDL1 was examined with quantitative reverse transcription (RT)-PCR. OsFRDL1 was mainly expressed in the roots, but not in the shoots (Fig. 6A). A time course experiment showed that the expression in the roots was not affected by Fe deficiency (Fig. 6, A and B), in contrast to IRT1, which expression was greatly increased with development of Fe deficiency (Fig. 6C). Interestingly, the expression of OsFRDL1 was higher in the mature root zone than in the root tip (Fig. 6D). This result is in agreement with Fe precipitation and development of xylem.
 ===Subcellular localization===
 ==Labs working on this gene==

← Older revision		Revision as of 07:26, 30 July 2016
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	===Expression Pattern===		===Expression Pattern===
		+	* The expression of OsFRDL1 was examined with quantitative reverse transcription (RT)-PCR. OsFRDL1 was mainly expressed in the roots, but not in the shoots (Fig. 6A). A time course experiment showed that the expression in the roots was not affected by Fe deficiency (Fig. 6, A and B), in contrast to IRT1, which expression was greatly increased with development of Fe deficiency (Fig. 6C). Interestingly, the expression of OsFRDL1 was higher in the mature root zone than in the root tip (Fig. 6D). This result is in agreement with Fe precipitation and development of xylem.
		+
	===Subcellular localization===		===Subcellular localization===

← Older revision		Revision as of 07:23, 30 July 2016
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	[[File:Os03g0216700-2.png\|center\|thumb\|400px\|'''Figure 4. Fe concentration in the shoots (A) and roots (B and C).''' ''<ref name="ref1" />.'']]		[[File:Os03g0216700-2.png\|center\|thumb\|400px\|'''Figure 4. Fe concentration in the shoots (A) and roots (B and C).''' ''<ref name="ref1" />.'']]
		+
		+	* The researchers compared the concentration of Fe and citrate in the xylem sap between ND and wild type at different Fe concentrations in the external solution. The concentration of citrate in the xylem sap of ND was less than one-half that of wild type at either external Fe concentration (Fig. 5A). However, there was no significant difference in malate concentration in the xylem sap between ND and wild type (Fig. 5B). The total Fe concentration in the xylem sap was significantly lower in ND than in wild type (Fig. 5C). In a separate experiment with 10 mM Fe, speciation analysis showed that there was no difference in Fe2+ concentration in the xylem sap between ND and wild type, but the concentration of Fe3+ in ND xylem sap was less than one-half that in wild type (Fig. 5D).
		+
		+	[[File:Os03g0216700-3.png\|center\|thumb\|400px\|'''Figure 5. Organic acids and Fe in the xylem sap.''' ''<ref name="ref1" />.'']]

	===Expression Pattern===		===Expression Pattern===