Os03g0285700 - Revision history

Xysj1981 at 04:41, 8 March 2017

2017-03-08T04:41:02Z

192.168.72.52: /* Structured Information */

2015-06-12T05:45:54Z

‎Structured Information

Sean: /* Function */

2014-06-10T12:10:30Z

‎Function

Sean: /* Function */

2014-06-10T12:08:56Z

‎Function

Trista: /* References */

2014-05-31T06:17:38Z

‎References

Trista: /* References */

2014-05-31T06:17:12Z

‎References

Trista: /* Expression */

2014-05-31T05:20:48Z

‎Expression

Trista: /* Function */

2014-05-31T05:20:32Z

‎Function

Trista: /* References */

2014-05-31T05:20:09Z

‎References

Trista: /* Expression */

2014-05-31T05:13:25Z

‎Expression

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os03g0285700''''' was reported as '''''OsAPXa''''' in 2010 <ref name="ref1" /> by researchers from Japan.
 ==Annotated Information==
 ===Function===
-OsAPX1 is a member of ascorbate peroxidase (APX) family. It is a haeme-containing enzyme of the ascorbate glutathione cycle that detoxifies reactive oxygen species in plants by catalyzing the conversion of hydrogen peroxide to water using ascorbate as a specific electron donor. The OsAPX1 expression is stress regulated[1].
+* '''''OsAPXa''''' is a member of ascorbate peroxidase (APX) family.
-OsAPX1 can affect the ability of rice plants to withstand chilling at the booting stage. When overexpress OsAPX1 in rice, it can increase APX activity which determine the antioxidant capacity. The levels of H2O2 and the MDA content increased by 1.5-fold and twofold, respectively in WT plants subjected to a 12℃ treatment for six days. In contrast, transgenic lines showed small changes in H2O2 levels and MDA content under cold stress, and H2O2 levels and MDA content were significantly lower than in WT plants. And the spikelet fertility was significantly higher in transgenic lines than in WT plants after a 12℃ treatment for six days. The reason is the APX activity enhances H2O2-scavenging capacity and protects spikelets, thereby increasing spikelet fertility under cold stress[2].
+* It is a haeme-containing enzyme of the ascorbate glutathione cycle that detoxifies reactive oxygen species in plants by catalyzing the conversion of hydrogen peroxide to water using ascorbate as a specific electron donor.
+* '''''OsAPXa''''' can affect the ability of rice plants to withstand chilling at the booting stage. When overexpress OsAPX1 in rice, it can increase APX activity which determine the antioxidant capacity.
-===Evolution===
+===Phenotypic analysis===
-Please input evolution information here.
+* To determine whether antioxidant capacity affects the ability of rice plants to withstand chilling at the booting stage, researhcers produced transgenic rice plants that overexpress '''''OsAPXa''''' and have increased APX activity.
 You can also add sub-section(s) at will.
 ==Labs working on this gene==
-Please input related labs here.
+* National Agricultural Research Center for Hokkaido Region, Hitsujigaoka 1, Toyohira, Sapporo 062-8555, Japan
 ==References==
-[1]Saurabh Pandey; Yogesh Kumar Negi,et al.Modeling and phylogenetic analysis of cytosolic ascorbate peroxidase (OsAPX1) from rice reveal signature motifs that may play a role in stress tolerance.Bioinformation,2014,10(3):119-123
+* <ref name="ref1">
+Sato Y, Masuta Y, Saito K, Murayama S, Ozawa K. Enhanced chilling tolerance at
-[2]Yutaka Sato;Yukari Masuta et al.Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa.Plant Cell Reports, 2011, 30(3): 399-406.
+the booting stage in rice by transgenic overexpression of the ascorbate
+peroxidase gene, OsAPXa. Plant Cell Rep. 2011 Mar;30(3):399-406. doi:
-[3]Ganesh Kumar Agrawal, Nam-Soo Jwac et al.Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.Gene, 2003, 322: 93-103.
+.1007/s00299-010-0985-7. PubMed PMID: 21203887.
+</ref>
-[4]Ting-Shao Chou;Yun-Yang Chao;Ching Huei Kao
+</references>
 ==Structured Information==

@@ Line 33: / Line 33: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 6: / Line 6: @@
 OsAPX1 can affect the ability of rice plants to withstand chilling at the booting stage. When overexpress OsAPX1 in rice, it can increase APX activity which determine the antioxidant capacity. The levels of H2O2 and the MDA content increased by 1.5-fold and twofold, respectively in WT plants subjected to a 12℃ treatment for six days. In contrast, transgenic lines showed small changes in H2O2 levels and MDA content under cold stress, and H2O2 levels and MDA content were significantly lower than in WT plants. And the spikelet fertility was significantly higher in transgenic lines than in WT plants after a 12℃ treatment for six days. The reason is the APX activity enhances H2O2-scavenging capacity and protects spikelets, thereby increasing spikelet fertility under cold stress[2].
- H2O2 may be involved in the regulation of HS- and Cd-increased APX and GR activities in leaves of rice seedlings.[4]
+H2O2 may be involved in the regulation of HS- and Cd-increased APX and GR activities in leaves of rice seedlings.[4]
 ===Expression===
@@ Line 27: / Line 27: @@
 [3]Ganesh Kumar Agrawal, Nam-Soo Jwac et al.Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.Gene, 2003, 322: 93-103.
 ==Structured Information==

@@ Line 5: / Line 5: @@
 OsAPX1 is a member of ascorbate peroxidase (APX) family. It is a haeme-containing enzyme of the ascorbate glutathione cycle that detoxifies reactive oxygen species in plants by catalyzing the conversion of hydrogen peroxide to water using ascorbate as a specific electron donor. The OsAPX1 expression is stress regulated[1].
 OsAPX1 can affect the ability of rice plants to withstand chilling at the booting stage. When overexpress OsAPX1 in rice, it can increase APX activity which determine the antioxidant capacity. The levels of H2O2 and the MDA content increased by 1.5-fold and twofold, respectively in WT plants subjected to a 12℃ treatment for six days. In contrast, transgenic lines showed small changes in H2O2 levels and MDA content under cold stress, and H2O2 levels and MDA content were significantly lower than in WT plants. And the spikelet fertility was significantly higher in transgenic lines than in WT plants after a 12℃ treatment for six days. The reason is the APX activity enhances H2O2-scavenging capacity and protects spikelets, thereby increasing spikelet fertility under cold stress[2].
 ===Expression===

@@ Line 21: / Line 21: @@
 ==References==
 [1]Saurabh Pandey; Yogesh Kumar Negi,et al.Modeling and phylogenetic analysis of cytosolic ascorbate peroxidase (OsAPX1) from rice reveal signature motifs that may play a role in stress tolerance.Bioinformation,2014,10(3):119-123
 [2]Yutaka Sato;Yukari Masuta et al.Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa.Plant Cell Reports, 2011, 30(3): 399-406.
 [3]Ganesh Kumar Agrawal, Nam-Soo Jwac et al.Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling.Gene, 2003, 322: 93-103.

@@ Line 7: / Line 7: @@
 ===Expression===
 Different APX isoforms are present in discrete subcellular compartments in rice and their expression is stress regulated.And OsAPX1 exist in the cytoplasm of rice.
 OsAPX1 show a weak constitutive expression in leaves. Their transcripts were up-regulated upon wounding (by cut), and diverse signals such as salicylic acid (SA), ethylene (using the ethylene generator, ethephon), abscisic acid (ABA), hydrogen peroxide, copper sulfate, protein phosphatase (PP) inhibitors, cantharidin (CN), endothall (EN) and okadaic acid (OA), and blast pathogen (Magnaporthe grisea) attack, but surprisingly not by jasmonic acid (JA).