Os03g0315400 - Revision history

Xysj2017 at 01:53, 14 February 2017

2017-02-14T01:53:40Z

Xysj2017: /* Subcellular localization */

2017-02-14T01:50:52Z

‎Subcellular localization

Xysj2017 at 01:50, 14 February 2017

2017-02-14T01:50:39Z

Xysj2017: /* Annotated Information */

2017-02-14T01:47:58Z

‎Annotated Information

Xysj2017: /* Expression */

2017-02-14T01:46:24Z

‎Expression

Xysj2017: /* Labs working on this gene */

2017-02-14T01:43:56Z

‎Labs working on this gene

Xysj2017 at 01:43, 14 February 2017

2017-02-14T01:43:17Z

Show changes

192.168.72.52: /* Structured Information */

2015-06-12T05:48:48Z

‎Structured Information

Lxzlxz at 08:44, 9 June 2014

2014-06-09T08:44:21Z

Lxzlxz at 08:40, 9 June 2014

2014-06-09T08:40:43Z

@@ Line 11: / Line 11: @@
 ===Phenotypic analysis===
 * No difference in growth and development between the OsMYB2-overexpressing and wild-type plants was observed under normal growth conditions, but the OsMYB2-overexpressing plants were more tolerant to salt, cold, and dehydration stresses and more sensitive to abscisic acid than wild-type plants.
-* The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H 2 O 2 and malondialdehyde.
+* The OsMYB2-overexpressing plants accumulated greater amounts of soluble sugars and proline than wild-type plants under salt stress. Overexpression of OsMYB2 enhanced up-regulation of genes encoding proline synthase and transporters. The OsMYB2-overexpressing plants accumulated less amounts of H2O2 and malondialdehyde.
 * The enhanced activities of antioxidant enzymes, including peroxidase, superoxide dismutase, and catalase, may underlie the lower H2O2 contents in OsMYB2-overexpressing plants.
 * There was greater up-regulation of stress-related genes, including OsLEA3, OsRab16A, and OsDREB2A, in the OsMYB2-overexpressing plants. Microarray analysis showed that expression of numerous genes involving diverse functions in stress response was altered in the OsMYB2-overexpressing plants.
@@ Line 20: / Line 20: @@
 ===Subcellular localization===
 * OsMYB2 was localized in the nucleus with transactivation activity.
-[[File:Subcellular localization of OsMYB2.jpg|center|thumb|800px|'''Figure 1.''' ''Subcellular localization and transactivation analysis of OsMYB2.<ref name="ref1" />.'']]
+[[File:Subcellular localization of OsMYB2.jpg|center|thumb|727px|'''Figure 1.''' ''Subcellular localization and transactivation analysis of OsMYB2.<ref name="ref1" />.'']]
 ===Evolution===

@@ Line 20: / Line 20: @@
 ===Subcellular localization===
 * OsMYB2 was localized in the nucleus with transactivation activity.
 [[File:Subcellular localization of OsMYB2.jpg|center|thumb|800px|'''Figure 1.''' ''Subcellular localization and transactivation analysis of OsMYB2.<ref name="ref1" />.'']]

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg|right|thumb|427px|'''Figure 2.''' ''Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.<ref name="ref1" />.'']]
+[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg|right|thumb|327px|'''Figure 2.''' ''Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.<ref name="ref1" />.'']]
 ===Gene Symbol===
@@ Line 17: / Line 17: @@
 ===Expression===
 * Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
 ===Subcellular localization===
@@ Line 23: / Line 22: @@
 <br>
 [[File:Subcellular localization of OsMYB2.jpg|center|thumb|800px|'''Figure 1.''' ''Subcellular localization and transactivation analysis of OsMYB2.<ref name="ref1" />.'']]
 ===Evolution===
-Phylogenetic tree of MYB proteins(figure 6).
+* OsMYB2 representative by '''''LOC_Os3g20090''''' ('''''Os03g0315400''''') is belong to C12, and of which are involved in stress response.
 ==Labs working on this gene==

@@ Line 2: / Line 2: @@
 ==Annotated Information==
 ===Gene Symbol===
 *'''''Os03g0315400''''' '''<=>''' '''''OsMYB2,MYB2'''''
@@ Line 15: / Line 17: @@
 ===Expression===
 * Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
 ===Subcellular localization===
@@ Line 26: / Line 29: @@
-[[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg]]
 ===Evolution===

@@ Line 15: / Line 15: @@
 ===Expression===
 * Expression of '''''OsMYB2''''' was up-regulated by salt, cold, and dehydration stress.
-a、b、c: localization of GFP ;  e、d、f:localization of GFP-OsMYB2
+===Subcellular localization===
 [[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg]]
 ===Evolution===

@@ Line 56: / Line 56: @@
 ==Labs working on this gene==
-* State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093,
+* State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, PR China
 * Graduate University of the Chinese Academy of Sciences, Beijing 100049, PR China

@@ Line 177: / Line 177: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 90: / Line 90: @@
 [[File:Effect of salt stress on contents of oxidants (A and B) and antioxidant enzymes (C–D) in wild-type and transgenic rice plants.jpg]]
 、''OsMYB2''-overexpression plants affect some genes' expression profiles. Some genes upregulation can contribute to enhanced tolerance of plants to salt stress and some may involved in stress response.
@@ Line 127: / Line 158: @@
 [[File:Real-time reverse-transcription (RT) PCR analysis for the expression of OsMYB2 in rice.jpg]]
 ===Evolution===

@@ Line 130: / Line 130: @@
 and transgenic rice were investigated and no significant differences
 in H2O2 and MDA contents were found in the absence
-of NaCl in the incubation medium (Fig.8A, B). There were
+of NaCl in the incubation medium (A, B). There were
 marked increases in H2O2 and MDA contents in both wildtype
 and transgenic plants upon exposure to 200 mM NaCl.
@@ Line 143: / Line 143: @@
 the OsMYB2-overexpressing lines resulted from differences
 in the activities of the major antioxidant enzymes. Under
-normal conditions, activities of POD, SOD, and CAT were
+normal conditions, activities of POD, SOD, and CAT were comparable among wild-type, OsMYB2-overexpressing,
 and RNAi plants (C-E). There were marked increases
 in activities of these enzymes for the rice seedlings upon
@@ Line 159: / Line 152: @@
 overexpressing plants, activities of these enzymes in the
 OsMYB2-RNAi lines did not differ from the wild-type
-plants under salt-stressed conditions (Fig.8C-E). These
+plants under salt-stressed conditions (C-E). These
 results imply that overexpression of OsMYB2 confers
 a more efficient antioxidant system to counteract oxidative