Os03g0356414 - Revision history

Guangyi: /* Annotated Information */

2016-12-14T05:27:06Z

‎Annotated Information

Guangyi at 05:24, 14 December 2016

2016-12-14T05:24:04Z

192.168.72.52 at 08:59, 16 June 2015

2015-06-16T08:59:45Z

192.168.72.52: /* Structured Information */

2015-06-12T05:53:10Z

‎Structured Information

Zhangzhang at 03:57, 25 May 2014

2014-05-25T03:57:24Z

Greenmood: /* Labs working on this gene */

2014-05-25T01:17:34Z

‎Labs working on this gene

Greenmood: /* References */

2014-05-24T11:23:01Z

‎References

Greenmood: /* Function */

2014-05-24T11:22:27Z

‎Function

Greenmood: /* Expression */

2014-05-24T11:15:01Z

‎Expression

Greenmood: /* Evolution */

2014-05-24T11:05:12Z

‎Evolution

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 <br>
 ===Function===
-Please input function information here.
+*'''OsDIS1''' plays a negative role in drought stress tolerance through transcriptional regulation of diverse stressrelated genes and possibly through posttranslational regulation of OsNek6 in rice.
+<br>
 ===Expression===
-Please input expression information here.
+*The expression of '''OsDIS1''' was up-regulated by drought treatment. In vitro ubiquitination assays showed that '''OsDIS1''' possessed E3 ubiquitin ligase activity and that the conserved region of the RING finger was required for the activity. Transient expression assays in Nicotiana benthamiana leaves and rice protoplasts indicated that '''OsDIS1''' was localized predominantly in the nucleus.
+<br>
 ===Evolution===
-Please input evolution information here.
+*Overexpression of '''OsDIS1''' reduced drought tolerance in transgenic rice plants, while RNA interference silencing of '''OsDIS1''' enhanced drought tolerance.
+<br>
 ==Labs working on this gene==

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+The rice '''Os03g0356414''' was reported as '''	OsDIS1''' in 2011 <ref name="ref1" /> by researchers from the China.
-Please input one-sentence summary here.
+<br>
 ==Annotated Information==
 ===Function===
 Please input function information here.
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 ==Labs working on this gene==
-Please input related labs here.
+*Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha Hunan 410128, China (Y.N., J.L., X.W., X.L., L.D., G.-L.W.);
+*State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China (Y.N., Q.Z., H.Z., L.L., S.T., Q.X.);
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==
 		 [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 03]]

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 Please input one-sentence summary here.
 ==Annotated Information==
 ===Function===
-OsDIS1 Negatively Regulates the Drought Response in Rice
+Please input function information here.
 ===Expression===
-To confirm the microarray expression results for OsDIS1, real-time PCR analysis was conducted with the OsDIS1 gene-specific primers using RNA isolated from 7-d-old seedlings before and after drought treatment. In the reactions, OsDREB2Awas used as a positive control for the drought
+Please input expression information here.
 ===Evolution===
-Among the analyzed 378 RING finger-type and 76 U-box-type E3 ubiquitin ligase genes in rice, a drought induced C3HC4 RING finger gene (LOC_Os03g24040) was identified.The deduced protein of this gene contains conserved RING finger and zinc finger motifs and has high sequence
+Please input evolution information here.
-===Global Gene Expression Analysis of the OsDIS1 Overexpression Plants under Normal and Drought-Stress Conditions===
+You can also add sub-section(s) at will.
 ==Labs working on this gene==
-#State Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing #China State Key Laboratory of Plant Genomics
+Please input related labs here.
 ==References==
-. Ning,Yuese and Jantasuriyarat.The SINA E3 ligase OsDIS1 negatively regulates drought response in rice.Plant physiology.2011,157:242--255；
+Please input cited references here.
 ==Structured Information==
+		 [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 03]]

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 identity (82%) with SINAT5 in Arabidopsis,which 2002), which was identified as a homolog of ''Drosophila'' SINA.A genome-wide search identified five paralogous SINA proteins of OsDIS1 in the rice genome. The amino acid identity between OsDIS1 and its paralogs is 91% with Os07g46560, 83% with Os02g19140, 83% with Os05g14860, 83% with Os01g13370, and 71% with Os02g03620. Further alignment between rice and Arabidopsis SINA proteins revealed that except for the N terminus, all the SINA members in rice and Arabidopsis had highly conserved sequences, and all contained the conserved RING finger and zinc finger motifs.Phylogenetic analysis showed that each of the following protein pairs belongs to the same clade: SINAT1 and SINAT2, SINAT4 and SINAT5, and OsDIS1 and Os07g46560; this supports the inference of a possible duplication of these genes in both genomes. Based on pairwise distance analysis, OsDIS1 has a minimal distance of 0.0725 with Os0746560 in rice and 0.2624 with SINAT5 in Arabidopsis,suggesting that Os07g46560 is the closest paralog of OsDIS1 in rice and SINAT5 is the closest ortholog of OsDIS1 in Arabidopsis.
 ===Global Gene Expression Analysis of the OsDIS1 Overexpression Plants under Normal and Drought-Stress Conditions===
 To identify genes involved in the OsDIS1-mediated drought-responsive pathway, we performed microarray analysis of the OsDIS1 overexpression and wildtype plants under both normal and drought-stress conditions using the Agilent rice Genechip. Sevenday-old plants of the OsDIS1 overexpression line 9-4-2 as well as the wild-type plants were used in the drought treatment. The expression of the droughtrelated gene OsLEA3 was highly induced, suggesting that the drought treatment was effective. The microarray analysis showed that 435 genes had at least a 2-fold change in the OsDIS1 overexpression plants compared with the wild-type plants under normal conditions. Among them, 135 genes were up-regulated and 300 genes were down-regulated. For example, Os05g18780, which encodes an RNase P-related protein, and Os01g46860, which encodes a high-affinity inorganic phosphate transporter 11 protein that is similar to OsPT4 were specifically induced in the overexpression plants. Meanwhile, 260 genes were specifically up-regulated and 255 genes were specifically down-regulated 2-fold in the overexpression plants compared with the wild-type plants under drought conditions. Many of these genes with differential expression under drought stress encode stress-related proteins and regulatory factors , such as protease inhibitor/seed storage/LTP family protein precursors, peroxidase precursors, universal stress proteins, amino acid metabolism enzymes and carrier proteins, transcription factors (WRKY, MYB, and AP2), and protein kinase.We then performed real-time PCR to confirm the differential expression of some of these genes under both normal and drought conditions. The analysis showed that Os01g46860 and Os05g18780 were specifically induced in the OsDIS1 overexpression plants under normal and drought conditions ). OsGRP2A and PIP2.3, whose Arabidopsis homologs act as negative regulators of drought respons ,were induced in the OsDIS1 overexpression plants only under drought conditions. Conversely, GST and OsMT-I-4b, whose homologs act as positive regulators of the drought response in Nicotiana tabacum and rice, respectively , were repressed in the OsDIS1 overexpression plants under drought conditions. Those results indicated that OsDIS1 negatively regulates the drought response by modulating the expression of diverse stress-related genes in rice.
 ==References==

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 . Lim, Sung Don and Hwang.Comprehensive Analysis of the Rice RING E3 Ligase Family Reveals Their Functional Diversity in Response to Abiotic stress.DNA research.2013,20:299--314；
 . Cho, Seok Keun and Ryu.The Arabidopsis RING E3 ubiquitin ligase AtAIRP2 plays combinatory roles with AtAIRP1 in abscisic acid-mediated drought stress responses.Plant physiology.2011,157:2240--2257
 ==Structured Information==

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 ===Expression===
-OsDIS1, a functional E3 ligase, interacts with an E2 (OsUBCx) and few substrates or partners to negatively regulates drought stress tolerance. Specifically,
+To confirm the microarray expression results for OsDIS1, real-time PCR analysis was conducted with the OsDIS1 gene-specific primers using RNA isolated from 7-d-old seedlings before and after drought treatment. In the reactions, OsDREB2Awas used as a positive control for the drought
-OsDIS1 interacts with a serine/threonine protein kinase OsNek6 and promotes its degradation via the 26S proteasome dependent pathway. In additional, OsDIS1 interacts with the drought and salt positive regulator OsSKIPa in rice.[[File:osDIS1.jpg]]
+treatment. Similar to OsDREB2A, OsDIS1 was highly induced 24 h after the drought treatment (Fig. 1B), suggesting that OsDIS1 is involved in the drought response in rice.
 ===Evolution===