Os03g0407400 - Revision history

Xysj2012: /* Function */

2016-12-30T05:35:21Z

‎Function

Xysj2012 at 05:32, 30 December 2016

2016-12-30T05:32:48Z

192.168.72.52: /* Structured Information */

2015-06-12T05:54:12Z

‎Structured Information

Cyyhappy1989: /* Annotated Information */

2014-05-17T06:46:06Z

‎Annotated Information

Cyyhappy1989: /* Annotated Information */

2014-05-17T06:42:35Z

‎Annotated Information

Cyyhappy1989: /* Annotated Information */

2014-05-17T06:32:04Z

‎Annotated Information

Cyyhappy1989: /* Annotated Information */

2014-05-17T06:24:08Z

‎Annotated Information

Cyyhappy1989: /* Annotated Information */

2014-05-17T06:20:15Z

‎Annotated Information

Cyyhappy1989: /* Function */

2014-05-17T06:12:41Z

‎Function

Cyyhappy1989: /* Function */

2014-05-17T05:55:35Z

‎Function

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 ===Function===
-• GS3, one of the genes regulating seed length, also regulates stigma length and participates in stigma exsertion in rice. GS3 mRNA is expressed in the basal  part of the young stigma, and a nonsense mutation in the second exon of GS3 causes an increase in cell number, resulting in elongation of the stigma.[1]  Figure 1  showed introgressed chromosome segment in AIS22 contributed to an increase in stigma length and, as a result, the frequency of stigma exsertion was increased by comparing AIS22 with Asominori and IR24.[1] A transgenic experiment (Figure 2) showed GS3 was the most likely
+* The rice '''''GS3''''' is a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein.
-candidate for the gene causing this difference between AIS22 and Asominori.[1]
+* Cloning of such a gene may provide the opportunity for fully characterizing the regulatory mechanism and related processes during grain development.
 ===Expression===

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os03g0407400''''' was reported as '''''GS3''''' in 2006 <ref name="ref1" /> by researchers from China.
 ===Function===
 • GS3, one of the genes regulating seed length, also regulates stigma length and participates in stigma exsertion in rice. GS3 mRNA is expressed in the basal  part of the young stigma, and a nonsense mutation in the second exon of GS3 causes an increase in cell number, resulting in elongation of the stigma.[1]  Figure 1  showed introgressed chromosome segment in AIS22 contributed to an increase in stigma length and, as a result, the frequency of stigma exsertion was increased by comparing AIS22 with Asominori and IR24.[1] A transgenic experiment (Figure 2) showed GS3 was the most likely

@@ Line 121: / Line 121: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 06:46, 17 May 2014
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	Student’sttest was used to generate thePvalues.		Student’sttest was used to generate thePvalues.

−	•RT–PCR and genetic transformation were used to analysis the expression of GS3. GS3 gene regulates grain size in rice during the early phases of panicle development while spikelets are elongating(Figure 8 A and B). GUS expression was observed in panicles up until 5 days before heading, but the signal was not detected in either flowering panicles or leaves(Figure 8 C and D).[4]	+	•RT–PCR and genetic transformation were used to analysis the expression of GS3. GS3 gene regulates grain size in rice during the early phases of panicle development while spikelets are elongating(Figure 7 A and B). GUS expression was observed in panicles up until 5 days before heading, but the signal was not detected in either flowering panicles or leaves(Figure 7 C and D).[4]
		+
		+	[[File:Figure 7.jpg]]
		+
		+	Figure 7: Expression analysis ofGS3using RT–PCR and
		+	genetic transformation. (A) RT–PCR ofGS3from Asominori,
		+	AIS22, and Nipponbare using young panicles (-3 cm) for
		+	RNA extraction. Numbers in parentheses indicate cycles of
		+	PCR. Actin cDNA was amplified as a control. (B) RT–PCR
		+	of GS3 from Asominori using panicles -3, 5, 7, 10, and
		+	13 cm and after heading and a flag leaf for RNA extraction.
		+	(C and D)GUSstaining of transgenic plants transformed with
		+	the GS3 promotor:GUSfusion construct showing panicles before flowering (C) and a panicle at flowering with a flag leaf
		+	(D). Bars, 1 cm.

@@ Line 58: / Line 58: @@
 before heading>10 cm in length; P5, panicle 5 d after heading; Pl, plumule
-•In order to investigate effects on grain size of the various domains assessed using transgenics, seven constructs were made by domain deletions(Figure 7A). VWFC domain seemed to have a larger effect of inhibiting OSR than did TNFR and that these two domains addedto each other in regulating OSR for grain size(Figure 7 A and B). Further, VWFC has a general role of inhibiting the effect of OSR in regulating plant growth and grain size(Figure 7 C, D, E and F).[3]
+•In order to investigate effects on grain size of the various domains assessed using transgenics, seven constructs were made by domain deletions(Figure 6 A). VWFC domain seemed to have a larger effect of inhibiting OSR than did TNFR and that these two domains addedto each other in regulating OSR for grain size(Figure 6 A and B). Further, VWFC has a general role of inhibiting the effect of OSR in regulating plant growth and grain size(Figure 6 C, D, E and F).[3]
 •RT–PCR and genetic transformation were used to analysis the expression of GS3. GS3 gene regulates grain size in rice during the early phases of panicle development while spikelets are elongating(Figure 8 A and B). GUS expression was observed in panicles up until 5 days before heading, but the signal was not detected in either flowering panicles or leaves(Figure 8 C and D).[4]

@@ Line 40: / Line 40: @@
 AIS22. Diagrams of each stigma are shown below the histogram. Error bars indicate SE (n=20).
-•GS3 was highly expressed in young panicle, and the signal gradually decreased with panicle development(Figure 6 A, B and C). GS3 was also highly expressed in root tips (Figure 6 D). Weak signals were observed in other tested tissues, including embryo, shoot apical meristem, leaf, and stem(Figure E, F, G A and H). GS3 mRNA preferentially accumulated in panicles less than 5 cm in length in both genotypes, whereas the transcript level was low in other tissues assayed(Figure 6 J). The expression level and pattern were not the cause of the loss of function of GS3in Minghui 63.[3]
+•GS3 was highly expressed in young panicle, and the signal gradually decreased with panicle development(Figure 5 A, B and C). GS3 was also highly expressed in root tips (Figure 5 D). Weak signals were observed in other tested tissues, including embryo, shoot apical meristem, leaf, and stem(Figure 5 E, F, G A and H). GS3 mRNA preferentially accumulated in panicles less than 5 cm in length in both genotypes, whereas the transcript level was low in other tissues assayed(Figure 5 J). The expression level and pattern were not the cause of the loss of function of GS3in Minghui 63.[3]
 •In order to investigate effects on grain size of the various domains assessed using transgenics, seven constructs were made by domain deletions(Figure 7A). VWFC domain seemed to have a larger effect of inhibiting OSR than did TNFR and that these two domains addedto each other in regulating OSR for grain size(Figure 7 A and B). Further, VWFC has a general role of inhibiting the effect of OSR in regulating plant growth and grain size(Figure 7 C, D, E and F).[3]

@@ Line 33: / Line 33: @@
 Figure 3: GUS staining of pistils transformed with the GS3promoter::GUSfusion construct and development of the stigma. (A) Pistils from panicles approximately 7 cm, 10 cm, 13 cm and 15 cm long and after heading. Scale bar, 500µm. (B) Relationship between panicle length and SNBP (stigma non-brush shaped part) length. Error bars indicate SE (n=4).
-•All the pistils from GS3 promoter::GUStransgenic plants showed the same GUS expression pattern. The period of greatest GS3 mRNA expression in the stigma coincided with the stage at which the stigma elongated dramatically(Figure. 4).[1] GS3 decreases the number of stigma cell(Figure 5).
+•All the pistils from GS3 promoter::GUStransgenic plants showed the same GUS expression pattern. The period of greatest GS3 mRNA expression in the stigma coincided with the stage at which the stigma elongated dramatically(Figure. 3).[1] GS3 decreases the number of stigma cell(Figure 4).
 •GS3 was highly expressed in young panicle, and the signal gradually decreased with panicle development(Figure 6 A, B and C). GS3 was also highly expressed in root tips (Figure 6 D). Weak signals were observed in other tested tissues, including embryo, shoot apical meristem, leaf, and stem(Figure E, F, G A and H). GS3 mRNA preferentially accumulated in panicles less than 5 cm in length in both genotypes, whereas the transcript level was low in other tissues assayed(Figure 6 J). The expression level and pattern were not the cause of the loss of function of GS3in Minghui 63.[3]

@@ Line 27: / Line 27: @@
 •A GS3 promoter::GUSfusion construct into Nipponbare and GUS staining at various stages of stigma development were used to estimate the mRNA expression of GS3in the pistil.[1]
-•GUS expression was observed in the basal part of young stigmas, and the strongest GUS expression was found in the basal part of stigmas and the upper part of styles. GUSexpression was not detected in the pistil (Figure. 4).
+•GUS expression was observed in the basal part of young stigmas, and the strongest GUS expression was found in the basal part of stigmas and the upper part of styles. GUSexpression was not detected in the pistil (Figure. 3).
 •All the pistils from GS3 promoter::GUStransgenic plants showed the same GUS expression pattern. The period of greatest GS3 mRNA expression in the stigma coincided with the stage at which the stigma elongated dramatically(Figure. 4).[1] GS3 decreases the number of stigma cell(Figure 5).

@@ Line 4: / Line 4: @@
 ==Annotated Information==
 ===Function===
-• GS3, one of the genes regulating seed length, also regulates stigma length and participates in stigma exsertion in rice. GS3 mRNA is expressed in the basal  part of the young stigma, and a nonsense mutation in the second exon of GS3 causes an increase in cell number, resulting in elongation of the stigma.[1]  Figure 1 and table 1 showed introgressed chromosome segment in AIS22 contributed to an increase in stigma length and, as a result, the frequency of stigma exsertion was increased by comparing AIS22 with Asominori and IR24.[1] A transgenic experiment (Figure 2 and Table 2) showed GS3 was the most likely
+• GS3, one of the genes regulating seed length, also regulates stigma length and participates in stigma exsertion in rice. GS3 mRNA is expressed in the basal  part of the young stigma, and a nonsense mutation in the second exon of GS3 causes an increase in cell number, resulting in elongation of the stigma.[1]  Figure 1  showed introgressed chromosome segment in AIS22 contributed to an increase in stigma length and, as a result, the frequency of stigma exsertion was increased by comparing AIS22 with Asominori and IR24.[1] A transgenic experiment (Figure 2) showed GS3 was the most likely
 candidate for the gene causing this difference between AIS22 and Asominori.[1]
 • The wild-type isoform is composed of four putative domains: a plant-specific organ size regulation (OSR) domain in the N terminus, a transmembrane domain,

@@ Line 6: / Line 6: @@
 • GS3, one of the genes regulating seed length, also regulates stigma length and participates in stigma exsertion in rice. GS3 mRNA is expressed in the basal  part of the young stigma, and a nonsense mutation in the second exon of GS3 causes an increase in cell number, resulting in elongation of the stigma.[1]  Figure 1 and table 1 showed introgressed chromosome segment in AIS22 contributed to an increase in stigma length and, as a result, the frequency of stigma exsertion was increased by comparing AIS22 with Asominori and IR24.[1] A transgenic experiment (Figure 2 and Table 2) showed GS3 was the most likely
 candidate for the gene causing this difference between AIS22 and Asominori.[1]
-[[File:.jpg]]
 • The wild-type isoform is composed of four putative domains: a plant-specific organ size regulation (OSR) domain in the N terminus, a transmembrane domain,
@@ Line 12: / Line 12: @@
 terminus. These domains function differentially in grain size regulation. The OSR domain is both necessary and sufficient for functioning as a negative regulator. The wild-type allele corresponds to medium grain. Loss of function of OSR results in long grain. The C-terminal TNFR/NGFR and VWFC domains show
 an inhibitory effect on the OSR function; loss-of function mutations of these domains produced very short grain.[3]
 ===Expression===