Os03g0666200 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T05:58:02Z

‎Structured Information

Sasniper: /* Evolution */

2014-06-07T02:34:22Z

‎Evolution

Sasniper: /* References */

2014-06-07T02:33:47Z

‎References

Sasniper: /* References */

2014-06-07T02:33:31Z

‎References

Sasniper at 02:29, 7 June 2014

2014-06-07T02:29:56Z

Sasniper: /* Function */

2014-06-07T02:28:09Z

‎Function

Sasniper at 02:25, 7 June 2014

2014-06-07T02:25:40Z

Sasniper: /* Labs working on this gene */

2014-06-07T02:18:37Z

‎Labs working on this gene

Sasniper: /* References */

2014-06-07T02:14:27Z

‎References

Sasniper: /* Evolution */

2014-06-07T02:08:23Z

‎Evolution

@@ Line 37: / Line 37: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 13: / Line 13: @@
 [[File:C.png|right|thumb|300px|'''Figure 3.'''Alignment of the amino acid sequences of the DH domains of OsSWAP70A and human SWAP70<ref name="ref2" />]]
 The PH domain contained four basic amino acids conserved in the PH domains of animal SWAP70 proteins, which are critical for binding to phosphatidylinositol 3,4,5-triphosphate (PIP3) <ref name="ref4" />. We also found an additional SWAP70 homologous gene (Os07g0138100) in rice by a BLAST search. Therefore, Os03g0666200 and Os07g0138100 were named OsSWAP70A and OsSWAP70B, respectively. OsSWAP70A and OsSWAP70B shared 64.2% identity (data not shown). A BLAST-based search for plant SWAP70 indicated that maize and sorghum have two genes, and there is one gene in Arabidopsis, ''Vitis vinifera'', ''Ricinus communis'', and ''Populus trichocarpa'' (Figure 4), suggesting that the SWAP70 homologs are conserved in the plant kingdom<ref name="ref2" />.
-[[File:Sasniper_D.png|right|thumb|300px|'''Figure 4.'''Phylogenetic tree of the SWAP70 family in plants. Accession numbers for plant SWAP70 genes are: ''Oryza sativa'' OsSWAP70A: AK102364; ''O. sativa'' OsSWAP70B: AK059496; ''Arabidopsis thaliana'' AtSWAP70: NP_850155; ''Vitis vinifera'' VvSWAP70: XP_002283267; ''Ricinus communis'' RcSWAP70: XP_002520368; ''Populus trichocarpa'' PtSWAP70: XP_002302134; ''Zea mays'' ZmSWAP70A: ACF87744; ''Z. mays'' ZmSWAP70B: NP_001151119; ''Sorghum bicolor'' SbSWAP70A: Sb01g012500; ''S. bicolor'' SbSWAP70B: Sb02g0026210<ref name="ref1" />.]]
+[[File:Sasniper_D.png|right|thumb|300px|'''Figure 4.'''Phylogenetic tree of the SWAP70 family in plants. Accession numbers for plant SWAP70 genes are: ''Oryza sativa'' OsSWAP70A: AK102364; ''O. sativa'' OsSWAP70B: AK059496; ''Arabidopsis thaliana'' AtSWAP70: NP_850155; ''Vitis vinifera'' VvSWAP70: XP_002283267; ''Ricinus communis'' RcSWAP70: XP_002520368; ''Populus trichocarpa'' PtSWAP70: XP_002302134; ''Zea mays'' ZmSWAP70A: ACF87744; ''Z. mays'' ZmSWAP70B: NP_001151119; ''Sorghum bicolor'' SbSWAP70A: Sb01g012500; ''S. bicolor'' SbSWAP70B: Sb02g0026210<ref name="ref2" />.]]
 ==Labs working on this gene==

@@ Line 22: / Line 22: @@
 ==References==
 <references>
-* <ref name="ref2">
+* <ref name="ref1">
 Yamaguchi K, Kawasaki T. Function of Arabidopsis SWAP70 GEF in immune response[J]. Plant signaling & behavior, 2012, 7(4): 465-468.
 </ref>

@@ Line 22: / Line 22: @@
 ==References==
 <references>
 * <ref name="ref2">
 Yamaguchi K, Imai K, Akamatsu A, et al. SWAP70 functions as a Rac/Rop guanine nucleotide‐exchange factor in rice[J]. The Plant Journal, 2012, 70(3): 389-397.

@@ Line 1: / Line 1: @@
-SWAP70, containing the DH domain, exhibited GEF activity toward the rice Rho GTPase OsRac1, and regulates chitin-induced production of reactive oxygen species and defense gene expression in rice.
+SWAP70, containing the DH domain, exhibited GEF activity toward the rice Rho GTPase OsRac1, and regulates chitin-induced production of reactive oxygen species and defense gene expression in rice<ref name="ref1" />.
 ==Annotated Information==
 ===Function===
@@ Line 5: / Line 5: @@
 ===Expression===
-[[File:Sasniper_E.jpg|right|thumb|300px|'''Figure 1.'''Visualization of OsSWAP70A and OsRac1 using bimolecular fluorescence complementation (BiFC) in transiently transfected rice protoplasts. Vn-OsRac1 and Vc-OsSWAP70A contain the N-terminal and C-terminal fragments, respectively, of Venus. Venus fluorescence indicates interaction between OsSWAP70A and OsRac1. The GUS protein was used as a negative control. Scale bar = 5 μm<ref name="ref1" />.]]
+[[File:Sasniper_E.jpg|right|thumb|300px|'''Figure 1.'''Visualization of OsSWAP70A and OsRac1 using bimolecular fluorescence complementation (BiFC) in transiently transfected rice protoplasts. Vn-OsRac1 and Vc-OsSWAP70A contain the N-terminal and C-terminal fragments, respectively, of Venus. Venus fluorescence indicates interaction between OsSWAP70A and OsRac1. The GUS protein was used as a negative control. Scale bar = 5 μm<ref name="ref2" />.]]
-The interaction ''in vivo'' between OsSWAP70A and OsRac1 in rice protoplasts were analysed by a bimolecular fluorescence complementation (BiFC) assay (Figure 1). OsRac1 tagged with the N-terminal domain [amino acids (aa) 1–154] of Venus (Vn-OsRac1) and OsSWAP70A tagged with the C-terminal domain (aa 155–238) (OsSWAP70A-Vc) were co-expressed in rice protoplasts together with the control red fluorescent protein (RFP). The Venus fluorescence was detected at the plasma membrane, whereas RFP was observed throughout the cytoplasm. These results indicate that OsRac1 and OsSWAP70A interacted with each other at the plasma membrane<ref name="ref1" />.
+The interaction ''in vivo'' between OsSWAP70A and OsRac1 in rice protoplasts were analysed by a bimolecular fluorescence complementation (BiFC) assay (Figure 1). OsRac1 tagged with the N-terminal domain [amino acids (aa) 1–154] of Venus (Vn-OsRac1) and OsSWAP70A tagged with the C-terminal domain (aa 155–238) (OsSWAP70A-Vc) were co-expressed in rice protoplasts together with the control red fluorescent protein (RFP). The Venus fluorescence was detected at the plasma membrane, whereas RFP was observed throughout the cytoplasm. These results indicate that OsRac1 and OsSWAP70A interacted with each other at the plasma membrane<ref name="ref2" />.
 ===Evolution===
-[[File:B.png|right|thumb|300px|'''Figure 2.'''Comparison of amino acid sequence between the PH domains of OsSWAP70A and human SWAP70<ref name="ref1" />]]
+[[File:B.png|right|thumb|300px|'''Figure 2.'''Comparison of amino acid sequence between the PH domains of OsSWAP70A and human SWAP70<ref name="ref2" />]]
-SWAP70 protein possessed a PH domain at the N-terminus of the putative DH domain, a characteristic of human SWAP70 Rho GEF<ref name="ref2" />. Both the putative PH and DH domains had 22% identity and 64% similarity to human SWAP70 (Figure 2,3)<ref name="ref1" />.
+SWAP70 protein possessed a PH domain at the N-terminus of the putative DH domain, a characteristic of human SWAP70 Rho GEF<ref name="ref3" />. Both the putative PH and DH domains had 22% identity and 64% similarity to human SWAP70 (Figure 2,3)<ref name="ref2" />.
-[[File:C.png|right|thumb|300px|'''Figure 3.'''Alignment of the amino acid sequences of the DH domains of OsSWAP70A and human SWAP70<ref name="ref1" />]]
+[[File:C.png|right|thumb|300px|'''Figure 3.'''Alignment of the amino acid sequences of the DH domains of OsSWAP70A and human SWAP70<ref name="ref2" />]]
-The PH domain contained four basic amino acids conserved in the PH domains of animal SWAP70 proteins, which are critical for binding to phosphatidylinositol 3,4,5-triphosphate (PIP3) <ref name="ref3" />. We also found an additional SWAP70 homologous gene (Os07g0138100) in rice by a BLAST search. Therefore, Os03g0666200 and Os07g0138100 were named OsSWAP70A and OsSWAP70B, respectively. OsSWAP70A and OsSWAP70B shared 64.2% identity (data not shown). A BLAST-based search for plant SWAP70 indicated that maize and sorghum have two genes, and there is one gene in Arabidopsis, ''Vitis vinifera'', ''Ricinus communis'', and ''Populus trichocarpa'' (Figure 4), suggesting that the SWAP70 homologs are conserved in the plant kingdom<ref name="ref1" />.
+The PH domain contained four basic amino acids conserved in the PH domains of animal SWAP70 proteins, which are critical for binding to phosphatidylinositol 3,4,5-triphosphate (PIP3) <ref name="ref4" />. We also found an additional SWAP70 homologous gene (Os07g0138100) in rice by a BLAST search. Therefore, Os03g0666200 and Os07g0138100 were named OsSWAP70A and OsSWAP70B, respectively. OsSWAP70A and OsSWAP70B shared 64.2% identity (data not shown). A BLAST-based search for plant SWAP70 indicated that maize and sorghum have two genes, and there is one gene in Arabidopsis, ''Vitis vinifera'', ''Ricinus communis'', and ''Populus trichocarpa'' (Figure 4), suggesting that the SWAP70 homologs are conserved in the plant kingdom<ref name="ref2" />.
 [[File:Sasniper_D.png|right|thumb|300px|'''Figure 4.'''Phylogenetic tree of the SWAP70 family in plants. Accession numbers for plant SWAP70 genes are: ''Oryza sativa'' OsSWAP70A: AK102364; ''O. sativa'' OsSWAP70B: AK059496; ''Arabidopsis thaliana'' AtSWAP70: NP_850155; ''Vitis vinifera'' VvSWAP70: XP_002283267; ''Ricinus communis'' RcSWAP70: XP_002520368; ''Populus trichocarpa'' PtSWAP70: XP_002302134; ''Zea mays'' ZmSWAP70A: ACF87744; ''Z. mays'' ZmSWAP70B: NP_001151119; ''Sorghum bicolor'' SbSWAP70A: Sb01g012500; ''S. bicolor'' SbSWAP70B: Sb02g0026210<ref name="ref1" />.]]
@@ Line 22: / Line 22: @@
 ==References==
 <references>
-* <ref name="ref1">
+* <ref name="ref2">
 Yamaguchi K, Imai K, Akamatsu A, et al. SWAP70 functions as a Rac/Rop guanine nucleotide‐exchange factor in rice[J]. The Plant Journal, 2012, 70(3): 389-397.
 </ref>
-* <ref name="ref2">
+* <ref name="ref3">
 Shinohara M, Terada Y, Iwamatsu A, et al. SWAP-70 is a guanine-nucleotide-exchange factor that mediates signalling of membrane ruffling[J]. Nature, 2002, 416(6882): 759-763.
 </ref>
-* <ref name="ref3">
+* <ref name="ref4">
 Oka, T., Ihara, S. and Fukui, Y. (2007) Cooperation of DEF6 with activated Rac in regulating cell morphology. J. Biol. Chem. 282, 2011–2018.
 </ref>

@@ Line 16: / Line 16: @@
 ==Labs working on this gene==
-Please input related labs here.
+* Department of Advanced Bioscience, Graduate School of Agriculture, Kinki University, 3327-204 Nakamachi, Nara 631-8505, Japan
 ==References==