Os03g0760200 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T06:02:40Z

‎Structured Information

Nengpan Zhang: /* References */

2014-06-04T14:19:06Z

‎References

Nengpan Zhang: /* Annotated Information */

2014-06-04T14:09:58Z

‎Annotated Information

Nengpan Zhang: /* Annotated Information */

2014-06-04T14:09:21Z

‎Annotated Information

Nengpan Zhang: /* Annotated Information */

2014-06-04T14:07:03Z

‎Annotated Information

Nengpan Zhang: /* Annotated Information */

2014-06-04T14:06:32Z

‎Annotated Information

Nengpan Zhang: /* Annotated Information */

2014-06-04T14:00:50Z

‎Annotated Information

Nengpan Zhang: /* Annotated Information */

2014-06-04T13:58:08Z

‎Annotated Information

Nengpan Zhang: /* Annotated Information */

2014-06-04T13:57:12Z

‎Annotated Information

Nengpan Zhang: /* Expression */

2014-06-04T13:43:12Z

‎Expression

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 <references>
 <ref name="ref1"> Pan, G., et al. (2006). Map-based cloning of a novel rice cytochrome P450 gene CYP81A6 that confers resistance to two different classes of herbicides. Plant Mol Biol 61(6): 933-943. </ref>
 </references>

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 [[File:FigureOs03g0760200_1.jpg|right|thumb|300px|'''Figure 1.''' '' Expression of ''CYP81A6'' in various organs of the rice (from reference <ref name="ref1" />).'']]
-[[File:FigureOs03g0760200_2.jpg|right|thumb|150px|'''Figure 2.''' '' Bentazon-sensitivity of two mutants (from reference <ref name="ref1" />).'']]
+[[File:FigureOs03g0760200_2.jpg|right|thumb|200px|'''Figure 2.''' '' Bentazon-sensitivity of two mutants (from reference <ref name="ref1" />).'']]
 ''CYP81A6'' gene is transcribed in all organs of rice and is highly expressed in shoots, leaf, leaf sheaths and immature panicles, while it is transcribed at low level in the roots (Figure 1)<ref name = "ref1"/>.

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 ==Annotated Information==
 ===Function===
 This gene also called ''CYP81A6'' gene, encodes a kind of protein named CYP81A6, which belongs to cytochrome P450 monooxygenases family<ref name = "ref1"/>. Cytochrome P450 monooxygenases are NADPH-dependent heme proteins that cast a large and diverse group of isozymers and mediate a wide arrange of oxidative reactions in plants, animals, and microorganisms<ref name = "ref1" />. In plants, cytochrome P450s mediate biosynthesis of lignins, terpenoid, and alkaloids, sterols,fatty acids, and many secondary compounds that act as plant defense agents. The protein coded by this gene confers resistance to two different kinds of  herbicides bentazon and sulfonylurea<ref name = "ref1"/>.
 ===Expression===
 [[File:FigureOs03g0760200_1.jpg|right|thumb|300px|'''Figure 1.''' '' Expression of ''CYP81A6'' in various organs of the rice (from reference <ref name="ref1" />).'']]
 ''CYP81A6'' gene is transcribed in all organs of rice and is highly expressed in shoots, leaf, leaf sheaths and immature panicles, while it is transcribed at low level in the roots (Figure 1)<ref name = "ref1"/>.
 ===Evolution===
 BLAST searches revealed that the deduced amino acid sequence of CYP81A6 was highly similar to that of the previously reported chortoluron hydroxylase CYP81B1 from Helianthus tuberrosus(45% identity and 63% similarity)<ref name = "ref4"/> and CYP81B2 from Nicotiana tabacum(44% identity and 59% similarity) <ref name = "ref5"/> and isoflavone hydroxylase CYP81E1 from Glycyrrhiza echinata(42% identity and 63% similarity)<ref name = "ref6"/> and CYP81E9 from Medicago truncatula(42% identity and 63% similarity)<ref name="ref7"/> nearly over the entire region.
 ===Mutaion===
-[[File:FigureOs03g0760200_2.jpg|right|thumb|100px|'''Figure 2.''' '' Bentazon-sensitivity of two mutants (from reference <ref name="ref1" />).'']]
+----
 Two bentazon-lethal mutants 8077S and Norin8m are obtained from γ-radiated ''indica'' cultivar W6154S<ref name = "ref2"/> and ''japonica'' cultivar Norin8<ref name = "ref3"/> respectively. ''CYP81A6'' from 8977S and Norin8m have a single-base deletion of G at the 2058th nucleotide and a single-base deletion of C at the 507th in its coding sequence respectively<ref name = "ref1"/>. Both 8077S and Norin8m plants started to show symptoms to bentazon at 100 mg/l and the lethal concentration was about 300 mg/l(Figure 2). The threshold concentrations of the bentazon sensitivity for the mutant plants were about 60-fold lower than those for their wild-type controls<ref name = "ref1"/>.

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 BLAST searches revealed that the deduced amino acid sequence of CYP81A6 was highly similar to that of the previously reported chortoluron hydroxylase CYP81B1 from Helianthus tuberrosus(45% identity and 63% similarity)<ref name = "ref4"/> and CYP81B2 from Nicotiana tabacum(44% identity and 59% similarity) <ref name = "ref5"/> and isoflavone hydroxylase CYP81E1 from Glycyrrhiza echinata(42% identity and 63% similarity)<ref name = "ref6"/> and CYP81E9 from Medicago truncatula(42% identity and 63% similarity)<ref name="ref7"/> nearly over the entire region.
 ===Mutaion===
-[[File:FigureOs03g0760200_2.jpg|right|thumb|300px|'''Figure 2.''' '' Bentazon-sensitivity of two mutants (from reference <ref name="ref1" />).'']]
+[[File:FigureOs03g0760200_2.jpg|right|thumb|100px|'''Figure 2.''' '' Bentazon-sensitivity of two mutants (from reference <ref name="ref1" />).'']]
 Two bentazon-lethal mutants 8077S and Norin8m are obtained from γ-radiated ''indica'' cultivar W6154S<ref name = "ref2"/> and ''japonica'' cultivar Norin8<ref name = "ref3"/> respectively. ''CYP81A6'' from 8977S and Norin8m have a single-base deletion of G at the 2058th nucleotide and a single-base deletion of C at the 507th in its coding sequence respectively<ref name = "ref1"/>. Both 8077S and Norin8m plants started to show symptoms to bentazon at 100 mg/l and the lethal concentration was about 300 mg/l(Figure 2). The threshold concentrations of the bentazon sensitivity for the mutant plants were about 60-fold lower than those for their wild-type controls<ref name = "ref1"/>.

@@ Line 10: / Line 10: @@
 ===Evolution===
-Please input evolution information here.
+BLAST searches revealed that the deduced amino acid sequence of CYP81A6 was highly similar to that of the previously reported chortoluron hydroxylase CYP81B1 from Helianthus tuberrosus(45% identity and 63% similarity)<ref name = "ref4"/> and CYP81B2 from Nicotiana tabacum(44% identity and 59% similarity) <ref name = "ref5"/> and isoflavone hydroxylase CYP81E1 from Glycyrrhiza echinata(42% identity and 63% similarity)<ref name = "ref6"/> and CYP81E9 from Medicago truncatula(42% identity and 63% similarity)<ref name="ref7" nearly over the entire region.
 ===Mutaion===
-Two bentazon-lethal mutants 8077S and Norin8m are obtained from γ-radiated ''indica'' cultivar W6154S<ref name = "ref2"/> and ''japonica'' cultivar Norin8<ref name = "ref3"/> respectively. ''CYP81A6'' from 8977S have a single-base deletion of G at the 2058th nucleotide in its coding sequence and ''CYP81A6'' from Norin8m have a single-base deletion of C at the 507th<ref name = "ref1"/>.
+[[File:FigureOs03g0760200_2.jpg|right|thumb|300px|'''Figure 2.''' '' Bentazon-sensitivity of two mutants (from reference <ref name="ref1" />).'']]
 ==Labs working on this gene==