Os03g0786400 - Revision history

Xysj2027: /* Labs working on this gene */

2017-05-07T16:14:16Z

‎Labs working on this gene

Xysj2027 at 10:56, 7 May 2017

2017-05-07T10:56:49Z

Xysj1981 at 06:16, 8 March 2017

2017-03-08T06:16:15Z

Tour: /* Annotated Information */

2014-05-19T16:08:10Z

‎Annotated Information

Tour: /* Expression */

2014-05-19T16:05:37Z

‎Expression

Tour: /* Annotated Information */

2014-05-19T15:57:16Z

‎Annotated Information

Tour: /* References */

2014-05-19T15:50:42Z

‎References

Tour: /* Labs working on this gene */

2014-05-19T15:49:36Z

‎Labs working on this gene

Tour: /* Evolution */

2014-05-19T15:48:53Z

‎Evolution

Tour: /* Function */

2014-05-19T15:33:14Z

‎Function

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 ==Labs working on this gene==
-* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of
+* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
 * State Key Laboratory of Hybrid Rice, China National Hybrid Rice Research and Development Center, Changsha 410125, China;
-* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China; and d Key
+* State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310006, China; * Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China
 ==References==

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-zinc finger protein is an important class of eukaryotic transcription factors
+The rice '''''Os03g0786400''''' was reported as '''''DST''''' in 2013 by researchers from China <ref name="ref1" /> .
 ==Annotated Information==
 ===Function===
-The Cys-2/His-2-type (C2H2) zinc finger protein—also called a classical or TFIIIA-type zinc finger—that was first found in Xenopus oocytes is an important class of eukaryotic transcription factors[1].
+* The zinc finger transcription factor DROUGHT AND SALT TOLERANCE ('''''DST''''') directly regulates OsCKX2 expression in the reproductive meristem.
-The phytohormone cytokinin (CK) positively regulates the activity and function of the shoot apical meristem (SAM), which is a major parameter determining seed production. The rice (Oryza sativa L.) Gn1a/OsCKX2 (Grain number 1a/Cytokinin oxidase 2) gene, which encodes a cytokinin oxidase, has been identified as a major quantitative trait locus contributing to grain number improvement in rice breeding practice. The zinc finger transcription factor DROUGHT AND SALT TOLERANCE (DST) directly regulates OsCKX2 expression in the reproductive meristem. DST-directed expression of OsCKX2 regulates CK accumulation in the SAM and, therefore, controls the number of the reproductive organs[2].
+* '''''DST'''''-directed expression of '''''OsCKX2''''' regulates CK accumulation in the SAM and, therefore, controls the number of the reproductive organs.
-And the zinc finger transcription factor  can negatively regulate stomatal closure by direct modulation of genes related to H2O2 homeostasis. Loss of DST function increases stomatal closure and reduces stomatal density, consequently resulting in enhanced drought and salt tolerance in rice[3].
+* A semidominant allele of the '''''DST''''' gene, perturbs '''''DST'''''-directed regulation of '''''OsCKX2''''' expression and elevates CK levels in the reproductive SAM, leading to increased meristem activity, enhanced panicle branching, and a consequent increase of grain number.
 ===Expression===
-DST is located in the nucleus with transcriptional activation and it will express momentarily down under the condition of salt stress and osmotic stress, then recovered to pre-treatment levels. All transgenic plants overexpressing a wild-type DST allele (DSTREG1) showed reduced plant stature with less panicle branches and decreased grain number (Fig. S1 A–D). In contrast, the DST RNA interference (RNAi) transgenic plants showed increased panicle branches and enhanced grain number（Fig.S1E–H）.
+* DST is located in the nucleus with transcriptional activation and it will express momentarily down under the condition of salt stress and osmotic stress, then recovered to pre-treatment levels.
 ==Labs working on this gene==
-China Natl Hybrid Rice Res & Dev Ctr, State Key Lab Hybrid Rice, Changsha 410125, Hunan, Peoples R China.
+* State Key Laboratory of Plant Genomics, National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of
+Sciences, Beijing 100101, China;
-Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China.
+* State Key Laboratory of Hybrid Rice, China National Hybrid Rice Research and Development Center, Changsha 410125, China;
 ==References==
-[1] Miller J, McLachlan AD, Klug A(1985) Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J 4:1609–1614.
+<references>
+<ref name="ref1">Shuyu Li;Bingran Zhao;Dingyang Yuan; Rice zinc finger protein DST enhances grain production through controlling Gn1a/OsCKX2 expression; Proceedings of the National Academy of Sciences, 2013, 110(8): 3167-3172</ref>
-[2] Shuyu Li;Bingran Zhao;Dingyang Yuan; Rice zinc finger protein DST enhances grain production through controlling Gn1a/OsCKX2 expression; Proceedings of the National Academy of Sciences, 2013, 110(8): 3167-3172
+<ref name="ref2"> Xin-Yuan Huang;Dai-Yin Chao; A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control; GENES & DEVELOPMENT, 2009, 23(15): 1805-1817</ref>
+<ref name="ref3"> Wolfe SA, Nekludova L, Pabo CO(2000) DNA recognition by Cys(2)His(2) zinc finger proteins. Annu Rev Biophys Biomol Struct 29:183–212.</ref>
-[3] Xin-Yuan Huang;Dai-Yin Chao; A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control; GENES & DEVELOPMENT, 2009, 23(15): 1805-1817
+<ref name="ref4"> Sakamoto H, Maruyama K, Sakuma Y, Meshi T, Iwabuchi M, Shinozaki K, Yamaguchi-Shinozaki K(2004) Arabidopsis Cys2/His2-type zinc-finger proteins function as transcription repressors under drought, cold, and high-salinity stress conditions. Plant Physiol 136:2734–2746.</ref>
+<ref name="ref5">  Lin R, Ding L, Casola C, Ripoll DR, Feschotte C, Wang H(2007) Transposase-derived transcription factors regulate light signaling in Arabidopsis. Science 318:1302–1305.</ref>
-[4] Wolfe SA, Nekludova L, Pabo CO(2000) DNA recognition by Cys(2)His(2) zinc finger proteins. Annu Rev Biophys Biomol Struct 29:183–212.
+</references>
 ==Structured Information==

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 zinc finger protein is an important class of eukaryotic transcription factors
 ==Annotated Information==
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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 Please input one-sentence summary here.
 ==Annotated Information==
 ===Function===

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 ===Expression===
-Please input expression information here.
+DST is located in the nucleus with transcriptional activation and it will express momentarily down under the condition of salt stress and osmotic stress, then recovered to pre-treatment levels. All transgenic plants overexpressing a wild-type DST allele (DSTREG1) showed reduced plant stature with less panicle branches and decreased grain number (Fig. S1 A–D). In contrast, the DST RNA interference (RNAi) transgenic plants showed increased panicle branches and enhanced grain number（Fig.S1E–H）.
 ===Evolution===

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 ===Evolution===
-Please input evolution information here.
+Generally, C2H2-type zinc finger proteins contain more than one zinc finger motif for DNA binding in the N terminus and separable transcriptional activation (or repression) domains in the C terminus [4]. The zinc finger motif is thought to recognize and bind its target DNA sequence, but has never been shown to be required for transcriptional activity [4,5,6].
 ==Labs working on this gene==