Os03g0788500 - Revision history

Xysj2012: /* Structured Information */

2015-05-15T05:44:10Z

‎Structured Information

Potatobing: /* Labs working on this gene */

2014-05-30T02:55:28Z

‎Labs working on this gene

Potatobing at 12:04, 21 May 2014

2014-05-21T12:04:54Z

Potatobing: /* Expression */

2014-05-21T11:55:53Z

‎Expression

Potatobing at 11:55, 21 May 2014

2014-05-21T11:55:14Z

Potatobing: /* Isolation and sequence analysis */

2014-05-21T08:35:32Z

‎Isolation and sequence analysis

Potatobing: /* Expression */

2014-05-21T08:34:19Z

‎Expression

Potatobing at 08:33, 21 May 2014

2014-05-21T08:33:42Z

Potatobing: /* Expression */

2014-05-21T08:30:06Z

‎Expression

Potatobing at 08:26, 21 May 2014

2014-05-21T08:26:12Z

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ==Labs working on this gene==
-Please input related labs here.
+State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China
 ==References==

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 ===Function===
 OsCPK10 is a member of OsCPKs family including 29 CPKs in rice[1]. Calcium-dependent protein kinases (CDPKs) are improtant calcium sensors icluded in plant responses to pathogen infection.  The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. Collectively, rice OsCPK10 is a crucial regulator in plant immune responses, and its overexpression may regulate disease resistance by activating both SA- and JA-dependent defense responses[2].
 ===Expression===
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-[[File:Os03g0788500expression.jpg]]
+[[File:Os03g0788500expression.jpg]][[File:expression2.jpg]]
 ===Isolation and sequence analysis===

@@ Line 7: / Line 7: @@
 ===Expression===
 The expression of OsCPK10, in M.grisea elicitor-treated rice calli, shows a rapid activation and peaks at 1 hour, which is earlier than that of OsPAL1 expression. This result indicates that OsCPK10 is an early pathogen-responsive gene[2].
 [[File:Os03g0788500expression.jpg]]

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 ==Annotated Information==
 ===Function===
-OsCPK10 is a member of OsCPKs family. Calcium-dependent protein kinases (CDPKs) are improtant calcium sensors icluded in plant responses to pathogen infection.  The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. Collectively, rice OsCPK10 is a crucial regulator in plant immune responses, and that it may regulate disease resistance by activating both SA- and JA-dependent defense responses.[1]
+OsCPK10 is a member of OsCPKs family including 29 CPKs in rice[1]. Calcium-dependent protein kinases (CDPKs) are improtant calcium sensors icluded in plant responses to pathogen infection.  The expression of OsCPK10 was strongly induced following treatment with a Magnaporthe grisea elicitor. Kinase activity assay showed that the functional OsCPK10 protein not only autophosphorylated, but also phosphorylated Casein in a calcium-dependent manner. Overexpression of constitutively active OsCPK10 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SA- and JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK10 exhibited enhanced resistance to blast fungus M. grisea. Collectively, rice OsCPK10 is a crucial regulator in plant immune responses, and its overexpression may regulate disease resistance by activating both SA- and JA-dependent defense responses[2].
 ===Expression===
-The expression of OsCPK10, in M.grisea elicitor-treated rice calli, shows a rapid activation and peaks at 1 hour, which is earlier than that of OsPAL1 expression. This result indicates that OsCPK10 is an early pathogen-responsive gene.[1]
+The expression of OsCPK10, in M.grisea elicitor-treated rice calli, shows a rapid activation and peaks at 1 hour, which is earlier than that of OsPAL1 expression. This result indicates that OsCPK10 is an early pathogen-responsive gene[2].
 ===Isolation and sequence analysis===
-OsCPK10 is isolated by PT-PCR from the total RNA extraction using specific primer pairs and the coding region of OsCPK10 is 1800 bp in length which encodes 599 amino acids. (Primers:forward 5'-ATGGGGAACACGTGCGTC-3' and reverse 5'-TCAAGACATCCTCAAGGCCTC-3'). The PCR products were ligated to a pEASY-T1 vector (TransGen Biotech, China).[1]
+OsCPK10 is isolated by PT-PCR from the total RNA extraction using specific primer pairs and the coding region of OsCPK10 is 1800 bp in length which encodes 599 amino acids. (Primers:forward 5'-ATGGGGAACACGTGCGTC-3' and reverse 5'-TCAAGACATCCTCAAGGCCTC-3'). The PCR products were ligated to a pEASY-T1 vector (TransGen Biotech, China)[2].
 ===Evolution===
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 ==References==
-[1] Fu L, Yu X, An C. Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiol Biochem. 2013 Dec;73:202-10. doi: 10.1016/j.plaphy.2013.10.004. Epub 2013 Oct 11. PubMed PMID: 24141028.
+[1] B. Wan, Y. Lin, T. Mou, Expression of rice Ca2+-dependent protein kinases (CDPKs) genes under different environmental stresses, FEBS Lett. 581 (2007)1179e1189
 ==Structured Information==

@@ Line 6: / Line 6: @@
 ===Expression===
-The expression of OsCPK10, in M.grisea elicitor-treated rice calli, shows a rapid activation and peaks at 1 hour, which is earlier than that of OsPAL1 expression. This result indicates that OsCPK10 is an early pathogen-responsive gene.
+The expression of OsCPK10, in M.grisea elicitor-treated rice calli, shows a rapid activation and peaks at 1 hour, which is earlier than that of OsPAL1 expression. This result indicates that OsCPK10 is an early pathogen-responsive gene. OsCPK10 is isolated by PT-PCR and the coding region of OsCPK10 is 1800 bp in length which encodes 599 amino acids.
-[[File:1.jpg]]
 ===Evolution===
 Please input evolution information here.