Os03g0843600 - Revision history

Liman: /* References */

2016-09-07T16:25:38Z

‎References

Liman: /* Function */

2016-09-07T16:24:42Z

‎Function

Liman at 16:19, 7 September 2016

2016-09-07T16:19:19Z

Liman at 16:17, 7 September 2016

2016-09-07T16:17:40Z

Liman at 16:05, 7 September 2016

2016-09-07T16:05:45Z

Liman at 16:04, 7 September 2016

2016-09-07T16:04:09Z

Liman at 16:02, 7 September 2016

2016-09-07T16:02:26Z

Liman: /* References */

2016-09-07T15:57:56Z

‎References

Liman: /* Evolution */

2016-09-07T15:57:09Z

‎Evolution

Liman: /* Labs working on this gene */

2016-09-07T15:37:26Z

‎Labs working on this gene

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 ZhuH, Riely BK, BurnsNJ, Ane JM(2006) Tracing nonlegume orthologs of legume genes required for nodulation and arbuscular mycorrhizal symbioses. Genetics 172: 2491–2499
 </ref>
 </references>
 ==Structured Information==
       [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 3]]

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 ==Annotated Information==
 ===Function===
-Please input function information here.
+* '''''Os-CASTOR''''' and '''''Os-POLLUX''''' are indispensable for mycorrhizal symbiosis in rice. The knockout of '''''Os-CASTOR''''' and '''''Os-POLLUX''''' has completely abolished the ability of the AM fungus to enter the plant root.
 ===Mutation===

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-Os-CASTOR was identified as Os03g62650 in the rice genome (Nipponbare) based on The Institute for Genomic Research Rice Genome Annotation.
+'''''Os-CASTOR''''' was identified as Os03g62650 in the rice genome (Nipponbare) based on The Institute for Genomic Research Rice Genome Annotation.
 ==Annotated Information==
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 ===Mutation===
-* The researchers identified one T-DNA insertion allele for Os-CASTOR in the genetic background of the japonica rice cv
+* The researchers identified one T-DNA insertion allele for '''''Os-CASTOR''''' in the genetic background of the japonica rice cv Hawyoung (line no. C04353), hereafter referred to as Os-castor-1. In the Os-castor-1 mutant, the T-DNA was inserted into the second 72-bp exon<ref name="ref1" />.
-* To test whether Os-CASTOR and Os-POLLUX are required for AM symbiosis in rice, we inoculated the mutant and wild-type rice roots with the fungus Glomus intraradices. At 35 d after inoculation, wildtype plants were densely colonized by G. intraradices, exhibiting the range of symbiotic structures typical of a functional symbiosis, including intercellular and intracellular hyphae, vesicles, and arbuscules. In each of the 60 wild-type plants from the genotypes Nipponbare, Hawyoung, and Dongjin, approximately 60% to 85% of the total root length was colonized. Similar levels of colonization also were observed for wild-type plants segregated from heterozygous mutant plants (Fig. 4). In contrast, intracellular fungal structures, including vesicles and arbuscules, were not observed on roots of 60 Os-castor-1, 72 Os-pollux-1, and 60 Ospollux- 2 homozygous mutant plants. For homozygous mutant plants, extraradical hyphae and appressoria were frequently observed on the root surface (Fig. 4), but the fungus was unable to penetrate the roots beyond the epidermis. These observations indicate that the knockout of Os-CASTOR and Os-POLLUX has completely abolished the ability of the AM fungus to enter the plant root<ref name="ref1" />.
+* To test whether '''''Os-CASTOR''''' and '''''Os-POLLUX''''' are required for AM symbiosis in rice, we inoculated the mutant and wild-type rice roots with the fungus Glomus intraradices. At 35 d after inoculation, wildtype plants were densely colonized by G. intraradices, exhibiting the range of symbiotic structures typical of a functional symbiosis, including intercellular and intracellular hyphae, vesicles, and arbuscules. In each of the 60 wild-type plants from the genotypes Nipponbare, Hawyoung, and Dongjin, approximately 60% to 85% of the total root length was colonized. Similar levels of colonization also were observed for wild-type plants segregated from heterozygous mutant plants (Fig. 4). In contrast, intracellular fungal structures, including vesicles and arbuscules, were not observed on roots of 60 Os-castor-1, 72 Os-pollux-1, and 60 Ospollux- 2 homozygous mutant plants. For homozygous mutant plants, extraradical hyphae and appressoria were frequently observed on the root surface (Fig. 4), but the fungus was unable to penetrate the roots beyond the epidermis. These observations indicate that the knockout of Os-CASTOR and Os-POLLUX has completely abolished the ability of the AM fungus to enter the plant root<ref name="ref1" />.
-[[File:Os03g0843600-2.png|center|thumb|400px|'''Figure 4. Os-castor and Os-pollux are defective in AM symbiosis. A to C, Roots of wild-type plants segregated from heterozygous mutants formed arbuscules upon inoculation with G. intraradices. D to F, Roots of homozygous mutants failed to form AM symbiosis, despite the presence of fungal hyphae on the root surface. Photographs were taken from roots at 5 weeks after inoculation with G. intraradices. Mycorrhizal colonization was assessed by trypan blue staining according to the procedures described by Koske and Gemma (1989). Stained roots were examined using a light microscope (Olympus BX40F-3), and images were captured by a microscope digital camera system (Olympus DP71). ap, Appressorium; ar, arbuscule; eh, extraradical hypha; ih, intraradical hypha.''' '' <ref name="ref1" />.'']]
+[[File:Os03g0843600-2.png|center|thumb|400px|'''Figure 4. '''''Os-castor''''' and '''''Os-pollux''''' are defective in AM symbiosis. A to C, Roots of wild-type plants segregated from heterozygous mutants formed arbuscules upon inoculation with G. intraradices. D to F, Roots of homozygous mutants failed to form AM symbiosis, despite the presence of fungal hyphae on the root surface. Photographs were taken from roots at 5 weeks after inoculation with G. intraradices. Mycorrhizal colonization was assessed by trypan blue staining according to the procedures described by Koske and Gemma (1989). Stained roots were examined using a light microscope (Olympus BX40F-3), and images were captured by a microscope digital camera system (Olympus DP71). ap, Appressorium; ar, arbuscule; eh, extraradical hypha; ih, intraradical hypha.''' '' <ref name="ref1" />.'']]
 ===Expression===
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 ===Evolution===
-* The between-species orthologous relationship of the CASTOR-POLLUX homologs can be readily inferred based on phylogenetic analysis (Fig. 1) and their microsyntenic genomic position<ref name="ref2" />.
+* The between-species orthologous relationship of the '''''CASTOR-POLLUX''''' homologs can be readily inferred based on phylogenetic analysis (Fig. 1) and their microsyntenic genomic position<ref name="ref2" />.
 [[File:Os03g0843600-1.png|center|thumb|400px|'''Figure 1. Phylogenetic tree (unrooted) of CASTOR and POLLUX homologs in M. truncatula (Mt), L. japonicus (Lj), soybean (Gm), poplar (Pt), grapevine (Vv), Arabidopsis (At), rice (Os), sorghum (Sb), and maize (Zm).''' '' <ref name="ref2" />.'']]

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 ===Function===
 Please input function information here.
 ===Expression===

← Older revision		Revision as of 16:05, 7 September 2016
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−	Os-CASTOR was identified as Os03g62650 in the rice genome (Nipponbare) based on The Institute for Genomic Research Rice Genome Annotation~~<ref name="ref1" />~~.	+	Os-CASTOR was identified as Os03g62650 in the rice genome (Nipponbare) based on The Institute for Genomic Research Rice Genome Annotation.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 16:04, 7 September 2016
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−	Os-CASTOR was identified as Os03g62650 in ~~2008~~<ref name="ref1" />.	+	Os-CASTOR was identified as Os03g62650 in the rice genome (Nipponbare) based on The Institute for Genomic Research Rice Genome Annotation<ref name="ref1" />.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 16:02, 7 September 2016
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−	~~Please input one~~-~~sentence summary here~~.	+	Os-CASTOR was identified as Os03g62650 in 2008<ref name="ref1" />.

	==Annotated Information==		==Annotated Information==

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 </ref>
 * <ref name="ref2">
+ZhuH, Riely BK, BurnsNJ, Ane JM(2006) Tracing nonlegume orthologs of legume genes required for nodulation and arbuscular mycorrhizal symbioses. Genetics 172: 2491–2499
 </ref>
 * <ref name="ref3">

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 ===Evolution===
-Please input evolution information here.
+* The between-species orthologous relationship of the CASTOR-POLLUX homologs can be readily inferred based on phylogenetic analysis (Fig. 1) and their microsyntenic genomic position<ref name="ref2" />.
-You can also add sub-section(s) at will.
+[[File:Os03g0843600-1.png|center|thumb|400px|'''Figure 1. Phylogenetic tree (unrooted) of CASTOR and POLLUX homologs in M. truncatula (Mt), L. japonicus (Lj), soybean (Gm), poplar (Pt), grapevine (Vv), Arabidopsis (At), rice (Os), sorghum (Sb), and maize (Zm).''' '' <ref name="ref2" />.'']]
 ==Labs working on this gene==

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 ==Labs working on this gene==
-Please input related labs here.
+* Department of Plant and Soil Sciences, University of Kentucky, Lexington, Kentucky 40546 Root
 ==References==