Os03g0848700 - Revision history

Xysj2012: /* Structured Information */

2015-05-15T05:41:11Z

‎Structured Information

Qinyue Zuo: /* Evolution */

2014-06-09T08:28:16Z

‎Evolution

Qinyue Zuo: /* Localization */

2014-06-09T08:27:17Z

‎Localization

Qinyue Zuo: /* Localization */

2014-06-09T08:24:22Z

‎Localization

Qinyue Zuo: /* Localization */

2014-06-09T08:22:17Z

‎Localization

Qinyue Zuo: /* Localization */

2014-06-09T07:40:45Z

‎Localization

Eniacgui: /* References */

2014-05-27T06:48:38Z

‎References

Eniacgui at 09:04, 25 May 2014

2014-05-25T09:04:45Z

Eniacgui: /* Annotated Information */

2014-05-25T08:56:22Z

‎Annotated Information

Eniacgui: /* Annotated Information */

2014-05-25T08:55:18Z

‎Annotated Information

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Evolution===
-Bph14 shares 83% sequence identity with its allele (Os03g0848700) in Nipponbare. Phylogenetic analysis revealed that Bph14 is closely related to other rice homologs and is divergent from the majority of known plant disease resistance proteins in other species. See Figure 3.
+Bph14 shares 83% sequence identity with its allele (Os03g0848700) in Nipponbare. Phylogenetic analysis revealed that Bph14 is closely related to other rice homologs and is divergent from the majority of known plant disease resistance proteins in other species. See Figure 5.
-[[File:Phylogenetic Analysis.png|250px|thumb|right|'''Figure 3. '''Phylogenetic relationships of ''Bph14'' homologs in rice (Os), wheat (Ta), cassava (Me), potato (Sb), tomato (Le), maize (Zm), barley (Hv), and Arabidopsis (At). (Scale bar, 0.1 amino acid substitutions per site.)  (from reference <ref name="ref1" />).]]
+[[File:Phylogenetic Analysis.png|250px|thumb|left|'''Figure 5. '''Phylogenetic relationships of ''Bph14'' homologs in rice (Os), wheat (Ta), cassava (Me), potato (Sb), tomato (Le), maize (Zm), barley (Hv), and Arabidopsis (At). (Scale bar, 0.1 amino acid substitutions per site.)  (from reference <ref name="ref1" />).]]
 ===Knowledge Extension===

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 ===Localization===
 Bph14 was expressed constitutively in leaf sheaths,leaf blades, and roots.See Figure 2. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes.See Figure 3.The ''Bph14''-GFP fusion protein is localized in the cy2oplasm. See Figure 4.
-[[File:Expression analysis of Bph14 in the root, leaf sheath, and leaf blade of rice by RT-PCR.jpg|left|thumb|332px|'''Figure 2.''' ''Bph14'' Expression analysis of Bph14. (from reference<ref name="ref1" />).]]
+[[File:Expression analysis of Bph14 in the root, leaf sheath, and leaf blade of rice by RT-PCR.jpg|left|thumb|272px|'''Figure 2.''' ''Bph14'' Expression analysis of Bph14. (from reference<ref name="ref1" />).]]
-[[File:Bph14 promoter–GUS expression pattern in transgenic rice plants. GUS express in the vascular system of root (E), leaf sheath (G), and leaf blade (I). Cross-sections of root (F), leaf sheath (H), and leaf blade (J) indicated .jpg|center|thumb|483px|'''Figure 3.''' ''Bph14'' promoter–GUS expression pattern in transgenic rice plants. (from reference<ref name="ref1" />).]]
+[[File:Bph14 promoter–GUS expression pattern in transgenic rice plants. GUS express in the vascular system of root (E), leaf sheath (G), and leaf blade (I). Cross-sections of root (F), leaf sheath (H), and leaf blade (J) indicated .jpg|center|thumb|683px|'''Figure 3.''' ''Bph14'' promoter–GUS expression pattern in transgenic rice plants. (from reference<ref name="ref1" />).]]
 [[File:Bph14-locolization.jpg|right|thumb|275px|'''Figure 4.''' ''Bph14'' subcellular localization. (from reference<ref name="ref1" />).]]

@@ Line 27: / Line 27: @@
 ===Localization===
 Bph14 was expressed constitutively in leaf sheaths,leaf blades, and roots.See Figure 2. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes.See Figure 3.The ''Bph14''-GFP fusion protein is localized in the cy2oplasm. See Figure 4.
-[[File:Expression analysis of Bph14 in the root, leaf sheath, and leaf blade of rice by RT-PCR.jpg|right|thumb|332px|'''Figure 2.''' ''Bph14'' Expression analysis of Bph14. (from reference<ref name="ref1" />).]]
+[[File:Expression analysis of Bph14 in the root, leaf sheath, and leaf blade of rice by RT-PCR.jpg|left|thumb|332px|'''Figure 2.''' ''Bph14'' Expression analysis of Bph14. (from reference<ref name="ref1" />).]]
-[[File:Bph14 promoter–GUS expression pattern in transgenic rice plants. GUS express in the vascular system of root (E), leaf sheath (G), and leaf blade (I). Cross-sections of root (F), leaf sheath (H), and leaf blade (J) indicated .jpg|right|thumb|483px|'''Figure 3.''' ''Bph14'' promoter–GUS expression pattern in transgenic rice plants. (from reference<ref name="ref1" />).]]
+[[File:Bph14 promoter–GUS expression pattern in transgenic rice plants. GUS express in the vascular system of root (E), leaf sheath (G), and leaf blade (I). Cross-sections of root (F), leaf sheath (H), and leaf blade (J) indicated .jpg|center|thumb|483px|'''Figure 3.''' ''Bph14'' promoter–GUS expression pattern in transgenic rice plants. (from reference<ref name="ref1" />).]]
 [[File:Bph14-locolization.jpg|right|thumb|275px|'''Figure 4.''' ''Bph14'' subcellular localization. (from reference<ref name="ref1" />).]]

@@ Line 26: / Line 26: @@
 ===Localization===
-Bph14 was expressed constitutively in leaf sheaths,leaf blades, and roots. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes.The ''Bph14''-GFP fusion protein is localized in the cytoplasm. See Figure 2.
+Bph14 was expressed constitutively in leaf sheaths,leaf blades, and roots.See Figure 2. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes. For examining Bph14 activity in more detail, using transgenic plants carrying the fusion construct of the Bph14 promoter region and the GUS reporter gene. The expression of GUS in transgenic plants was detected mainly in the vascular tissue of various organs, including leaf sheaths and leaf blades. In cross-sections of these organs, GUS activity was strongly detected in the parenchyma cells bordering, xylem vessels and sieve tubes.See Figure 3.The ''Bph14''-GFP fusion protein is localized in the cy2oplasm. See Figure 4.
-[[File:Bph14-locolization.jpg|right|thumb|275px|'''Figure 2.''' ''Bph14'' subcellular localization. (from reference<ref name="ref1" />).]]
+[[File:Expression analysis of Bph14 in the root, leaf sheath, and leaf blade of rice by RT-PCR.jpg|right|thumb|332px|'''Figure 2.''' ''Bph14'' Expression analysis of Bph14. (from reference<ref name="ref1" />).]]
 ===Evolution===

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 ==References==
 <references>
-<ref name="ref1">Bo Dua, Weilin Zhanga,Bingfang Liua, et al. (2009) Identification and characterization of Bph14, a gene conferring resistance to brown planthopper in rice.Proceedings of the National Academy of Sciences, 106(52): 22163-22168.</ref>
+<ref name="ref1">Bo Dua, Weilin Zhanga,Bingfang Liua, et al. (2009) Identification and characterization of ''Bph14'', a gene conferring resistance to brown planthopper in rice.Proceedings of the National Academy of Sciences, 106(52): 22163-22168.</ref>
 <ref name="ref2">Huang Z, He GC, Shu LH, et al. (2001) Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet 102:929–934.</ref>
 <ref name="ref3">Jie Li, Qiuhong Chen, Liangquan Wang, et al. (2011) Biological effects of rice harbouring ''Bph14'' and ''Bph15'' on brown planthopper, ''Nilaparvata lugens''. Pest Management Science, 67:528–534.</ref>

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 ===Knowledge Extension===
-Plant defense responses to insects include the activation of pathways dependent on SA and JA/ethylene signaling molecules. There was no significant difference between the transgenic and the wild-type plants in transcript levels of the JA synthesis-related genes LOX(lipoxygenase) and AOS2 (allene oxide synthase 2) in 24 h after BPH infestation. At all subsequent time points, however, transcript levels of these genes were substantially lower in the transgenics than in wildtype plants. In addition, the ethylene signaling pathway receptor gene EIN2(ethylene insensitive 2) accumulated faster and at higher levels in the wild type than in transgenics. These results suggested that BPH feeding induced the defenses in the susceptible plants associated with a JA/ethylene-dependent pathway. However, transcript levels of the SA synthesis-related genes EDS1(enhanced disease susceptibility 1), PAD4(phytoalexin deficient 4),PAL(phenylalanine ammonia-lyase), and ICS1 (isochorismate synthase 1) were higher in the transgenic
+Plant defense responses to insects include the activation of pathways dependent on SA and JA/ethylene signaling molecules. There was no significant difference between the transgenic and the wild-type plants in transcript levels of the JA synthesis-related genes LOX(lipoxygenase) and AOS2 (allene oxide synthase 2) in 24 h after BPH infestation. At all subsequent time points, however, transcript levels of these genes were substantially lower in the transgenics than in wildtype plants. In addition, the ethylene signaling pathway receptor gene EIN2(ethylene insensitive 2) accumulated faster and at higher levels in the wild type than in transgenics. These results suggested that BPH feeding induced the defenses in the susceptible plants associated with a JA/ethylene-dependent pathway. However, transcript levels of the SA synthesis-related genes EDS1(enhanced disease susceptibility 1), PAD4(phytoalexin deficient 4), PAL(phenylalanine ammonia-lyase), and ICS1 (isochorismate synthase 1) <ref name="ref5" /> were higher in the transgenic plants than in wild-type plants after BPH infestation, suggesting that Bph14may activate an SA-dependent resistance pathway after BPH feeding. NPR1(homolog of Arabidopsis nonexpressor of pathogenesis-related genes 1) is a key regulator of SAdependent systemic acquired resistance (SAR) that was found to enhance the expression of PR1b(basic pathogenesis-related gene 1)<ref name="ref6" />. PR1bis suggested to be effective in inhibiting pathogen growth, multiplication, and/or spread and is responsible for the SAR in plants. The transcript level ofNPR1was also higher in the transgenic than in the wild-type plants. However, the higher transcript level of NPR1did not result in stronger induction of PR1b,suggestingthatthetranscriptlevelofPR1b was regulated by JA/ethylene signal in rice after BPH infestation. These results demonstrate thatBph14activates a NPR1-dependent, butPR1-independent, resistance after BPH feeding.
 ==Labs working on this gene==
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 <ref name="ref3">Jie Li, Qiuhong Chen, Liangquan Wang, et al. (2011) Biological effects of rice harbouring ''Bph14'' and ''Bph15'' on brown planthopper, ''Nilaparvata lugens''. Pest Management Science, 67:528–534.</ref>
 <ref name="ref4">Zhou Lei, Zhijun Chen, Xuyong Lang, et al. (2013) Development and validation of a PCR-based functional marker system for the brown planthopper resistance gene Bph14 in rice. Breeding Science, 63:347–352.</ref>
 </references>