Os04g0401000 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:02:55Z

‎Structured Information

Rainrainnnn at 08:36, 23 May 2014

2014-05-23T08:36:22Z

Rainrainnnn: /* Expression */

2014-05-23T08:28:56Z

‎Expression

Rainrainnnn: /* Function */

2014-05-23T08:27:08Z

‎Function

Rainrainnnn: /* Function */

2014-05-23T08:26:32Z

‎Function

Rainrainnnn: /* Expression */

2014-05-23T08:22:18Z

‎Expression

Rainrainnnn: /* Function */

2014-05-23T08:21:56Z

‎Function

Rainrainnnn at 08:11, 23 May 2014

2014-05-23T08:11:42Z

Rainrainnnn at 08:10, 23 May 2014

2014-05-23T08:10:08Z

Rainrainnnn at 07:48, 23 May 2014

2014-05-23T07:48:58Z

@@ Line 45: / Line 45: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 15: / Line 15: @@
 ===Expression===
 The full lenth of　cDNA of wild-type '''''Pi21''''' is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids  containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of '''''Pi21''''' gene in resistant varieties ''Owarihatamochi'', which is the cause of different resistance<ref name="ref2" />.
 ==Labs working on this gene==

@@ Line 18: / Line 18: @@
 [[File:QQ截图20140523154307.jpg|right|thumb|250px|'''Figure 4.''' ''Molecular characterization of Pi21.(from reference<ref name="ref2" />).'']]
-The full lenth of　cDNA of wild-type '''''Pi21''''' is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids  containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of '''''Pi21''''' gene in resistant varieties Owarihatamochi, which is the cause of different resistance<ref name="ref2" />.
+The full lenth of　cDNA of wild-type '''''Pi21''''' is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids  containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of '''''Pi21''''' gene in resistant varieties ''Owarihatamochi'', which is the cause of different resistance<ref name="ref2" />.
 [[File:QQ截图20140523154328.jpg|right|thumb|250px|'''Figure 4.''' ''Successful introduction of resistant pi21 allele into an elite cultivar. (from reference<ref name="ref2" />).'']]

@@ Line 5: / Line 5: @@
 [[File:QQ截图20140523142047ddddd.jpg|right|thumb|250px|'''Figure 1.''' ''The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. (from reference<ref name="ref1" />).'']]
-'''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of '''''Pi21''''' is showed on the right<ref name="ref1" />.
+'''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers<ref name="ref1" />. The location of '''''Pi21''''' is showed on the right.
 [[File:QQ截图20140523143030.jpg|right|thumb|250px|'''Figure 2.''' ''Phenotypic characterization of the resistant pi21 allele.(from reference<ref name="ref2" />).'']]

@@ Line 5: / Line 5: @@
 [[File:QQ截图20140523142047ddddd.jpg|right|thumb|250px|'''Figure 1.''' ''The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. (from reference<ref name="ref1" />).'']]
-'''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of '''''Pi21''''' is showed on the right. The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. The locations of RFLP marker loci and a gene for field resistance,
+'''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of '''''Pi21''''' is showed on the right<ref name="ref1" />.
 [[File:QQ截图20140523143030.jpg|right|thumb|250px|'''Figure 2.''' ''Phenotypic characterization of the resistant pi21 allele.(from reference<ref name="ref2" />).'']]

@@ Line 24: / Line 24: @@
 ==Labs working on this gene==
 .QTL Genomics Research Center, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 3058602, Japan.
 .Mountainous Region Agricultural Research Institute, Aichi Agricultural Research Center, Inahasi, Toyota, Aichi 4412513, Japan.
 Bioproduction Sciences, Ishikawa Prefectural University, Suematsu 1-308, Nonoichi-machi, Ishikawa 921-8836, Japan.
 .Institute of the Society for Techno-Innovation of Agriculture, Forestry and Fisheries, Ippaizuka, Tsukuba, Ibaraki 3050854, Japan.
 .Division of Plant Sciences, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 3058602, Japan.
 .Division of Genome and Biodiversity Research, National Institute of Agrobiological Sciences, Kannondai 2-1-2,Tsukuba, Ibaraki 305-8602, Japan.
 .Graduate School of Life and Environmental Sciences, University of Tsukuba, Tennohdai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.

@@ Line 1: / Line 1: @@
 Os04g0401000 is known as Pyricularia oryzae resistance-21('''''Pi-21''''') gene.It is quantitative trait loci (QTLs) conferring field resistance to rice blast.
 ==Annotated Information==
-[[File:QQ截图20140523142047ddddd.jpg]]
+[[File:QQ截图20140523142047ddddd.jpg|right|thumb|250px|'''Figure 1.''' ''The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. (from reference<ref name="ref1" />).'']]
 '''''Pi21''''' was identifed in ''Owarihatamochi'' (Japanese upland rice).The resistance gene, designated pi21, was mapped on chromosome 4 as a single recessive gene between RFLP marker loci G271 and G317 at a distance of 5.0 cM and 8.5 cM, respectively,using RFLP and SSR markers.the location of '''''Pi21''''' is showed on the right. The introgressions on chromosome 4 from Owarihatamochi in two BC1 F1 plants, 96BC131 (A) and 96BC122 (B), respectively. The locations of RFLP marker loci and a gene for field resistance, pi21, are indicated with an open ellipse. Genetic distances among them in BC1 F3 populations are shown right on right in centimorgans. The map of rice chromosome 4  is shown on the left, indicating the location of putative QTLs with shaded boxes<ref name="ref1" />.
-===Function===
+[[File:QQ截图20140523143030.jpg|right|thumb|250px|'''Figure 2.''' ''Phenotypic characterization of the resistant pi21 allele.(from reference<ref name="ref2" />).'']]
 '''''Pi21''''' encodes a proline-rich protein that includes a putative heavy metal–binding domain and putative protein-protein interaction motifs<ref name="ref1" />. Wild-type '''''Pi21''''' appears to slow the plant’s defense responses, which may support optimization of defense mechanisms. Deletions in its proline-rich motif inhibit this slowing<ref name="ref2" />. '''''Pi21''''' is separable from a closely linked gene conferring poor flavor. The resistant pi21 allele, which is found in some strains of japonica rice, could improve blast resistance of rice worldwide<ref name="ref2" />.
 Leaves of AA-pi21, a near-isogenic line (NIL) carrying '''''Pi21''''' in the genetic background of a susceptible cultivar, had smaller lesions than did the susceptible cultivar (AA) in both a field evaluation and a greenhouse inoculation test. The resistant '''''Pi21''''' allele had a consistent effect against all 10 of the widely distributed races of studied, although the resistance was incomplete as compared with that triggered by '''''R''''' genes.Histological examination showed that cells infected with a virulent race were still alive and intact in both AA and AA-pi21 40 hours after inoculation, in contrast to those infected with an avirulent race, which induces '''''R''''' gene–dependent hypersensitive cell death. However, cytoplasmic granules, the first sign of cell death, were frequently observed in AA-pi21 96 hours after inoculation .The rate of penetration of hyphae into host cells in AA-pi21 did not differ from that in AA, but the rate of invasion of hyphae from penetrated cells into adjacent cells, as an indicator of hyphal growth, was significantly lower in AA-pi21 , which is consistent with the observations in the greenhouse inoculation test. These observations suggest that '''''Pi21''''' confers non–race-specific resistance, which is possibly associated with a slow induction of resistance.The resistant '''''Pi21''''' allele did not affect resistance to other fungal and bacterial pathogens<ref name="ref2" />.
-[[File:QQ截图20140523145616.jpg]]
+[[File:QQ截图20140523154252.jpg|right|thumb|250px|'''Figure 3.''' ''Genetic and physical maps of Pi21.(from reference<ref name="ref2" />).'']]
 ===Expression===
-[[File:QQ截图20140523150944.jpg]]
+[[File:QQ截图20140523154307.jpg|right|thumb|250px|'''Figure 4.''' ''Molecular characterization of Pi21.(from reference<ref name="ref2" />).'']]
 The full lenth of　cDNA of ｗild-type '''''Pi21''''' is 1109bp, which includes 3 exons，Encoding a proline-rich protein product of 266 amino acids  containing heavy metal binding domain and protein-protein interaction domains and other functional domains. Compared with the susceptible cultivar, there are 21bp and 48bp missing of '''''Pi21''''' gene in resistant varieties Owarihatamochi, which is the cause of different resistance<ref name="ref2" />.
-[[File:QQ截图20140523151005.jpg]]
+[[File:QQ截图20140523154328.jpg|right|thumb|250px|'''Figure 4.''' ''Successful introduction of resistant pi21 allele into an elite cultivar. (from reference<ref name="ref2" />).'']]
 ==Labs working on this gene==