Os04g0409600 - Revision history

192.168.72.52 at 07:03, 12 June 2015

2015-06-12T07:03:15Z

Shfengna: /* References */

2014-06-05T11:42:21Z

‎References

Shfengna: /* Function */

2014-06-05T11:39:57Z

‎Function

Lina: /* References */

2014-06-05T11:14:21Z

‎References

Lina: /* Expression */

2014-06-05T11:12:51Z

‎Expression

Lina: /* Expression */

2014-06-05T11:12:00Z

‎Expression

Lina: /* References */

2014-06-05T11:09:04Z

‎References

Lina: /* Function */

2014-06-05T11:06:50Z

‎Function

Jc0407: /* References */

2014-06-05T08:49:14Z

‎References

Jc0407: /* Expression */

2014-06-05T08:46:58Z

‎Expression

@@ Line 119: / Line 119: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 11:42, 5 June 2014
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	<ref name="ref15">Huang L, Sun Q, Qin F, Li C, Zhao Y, et al. (2007) Down-regulation of a SILENT INFORMATION REGULATOR2-related histone deacetylase gene, OsSRT1, induces DNA fragmentation and cell death in rice. Plant Physiology 144: 1508–1519.</ref>		<ref name="ref15">Huang L, Sun Q, Qin F, Li C, Zhao Y, et al. (2007) Down-regulation of a SILENT INFORMATION REGULATOR2-related histone deacetylase gene, OsSRT1, induces DNA fragmentation and cell death in rice. Plant Physiology 144: 1508–1519.</ref>
		+
		+	<ref name="ref16">Hu Y, Liu D, Zhong X, Zhang C, Zhang Q, et al. (2012) CHD3 protein recognizes and regulates methylated histone H3 lysines 4 and 27 over a subset of targets in the rice genome. Proceedings of the National Academy of Sciences of the United States of America 109: 5773–5778.</ref>

← Older revision		Revision as of 11:39, 5 June 2014
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	*Histone lysine acetylation is an important epigenetic modification for genome function and gene activity. Dynamic modulation of histone acetylation in plants has been shown to be involved in gene expression programs of plant development and responses to environmental conditions <ref name="ref13" />.''<ref name="ref14" />.''		*Histone lysine acetylation is an important epigenetic modification for genome function and gene activity. Dynamic modulation of histone acetylation in plants has been shown to be involved in gene expression programs of plant development and responses to environmental conditions <ref name="ref13" />.''<ref name="ref14" />.''
		+
		+
		+	*The homeostasis of histone H3K9 acetylation regulated by OsSRT1 in controlling genome-wide gene expression remained unclear. It was not known whether OsSRT1 directly targeted to specific genes to undergo histone deacetylation. To study the chromatin function and to identify direct binding targets of the OsSRT1, we first compared the genome-wide H3K9 acetylation profiles between wild type and OsSRT1 down-regulation plants, and then investigated the genomic binding sites of OsSRT1 by chromatin immunoprecipitation assays.OsSRT1 down-regulation did not drastically alter the genome-wide H3K9ac landscape, but led to increases of H3K9ac over a subset of genes belonging to specific functional categories and transposable elements. OsSRT1 was found to be associated with 1824 genes that showed relatively low levels of H3K9ac. <ref name="ref16" />.''

	===Expression===		===Expression===

@@ Line 104: / Line 104: @@
 <ref name="ref12"> Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J 11: 1187–1194</ref>
-<ref name="ref13">Servet C, Conde E, Silva N, Zhou D-X (2010) Histone acetyltransferase AtGCN5/HAG1 is a versatile regulator of developmental and inducible gene expression in Arabidopsis. Molecular plant 3: 670–677.</ref>
+<ref name="ref13">Servet C, Conde E, Silva N, Zhou D-X (2010) Histone acetyltransferase AtGCN5/HAG1 is a versatile regulator of developmental and inducible gene expression in Arabidopsis. Molecular plant 3: 670–677</ref>
 <ref name="ref14">Chen ZJ, Tian L (2007) Roles of dynamic and reversible histone acetylation in plant development and polyploidy. Biochimica et Biophysica Acta 1769: 295–307.</ref>
 </references>

@@ Line 50: / Line 50: @@
 *We compared the expression of two hypersensitive response (HSR201 and HSR203J) marker genes <ref name="ref9" />.''and a cytochromeP450 gene (calledAPO) that is closely related to wheat (Triticum aestivum) CYP709C1 and CYP709C3v2, both of which are suggested to be involved in wheat defense to pathogens <ref name="ref10" />.''<ref name="ref11" />.''
 *Down-regulation of OsSRT1 by RNAi produces a lesion-mimic phenotype and induces H3K9 acetylation (H3K9ac) and expression of hypersensitive response-related genes in rice plants. In addition, the transcription of many transposable elements is activated in the RNAi plants, indicating
-that transposons and cell death-related genes might be among the primary targets of OsSRT1-mediated gene silencing.<ref name="ref15" />.''
+*Down-regulation of OsSRT1 by RNAi produces a lesion-mimic phenotype and induces H3K9 acetylation (H3K9ac) and expression of hypersensitive response-related genes in rice plants. In addition, the transcription of many transposable elements is activated in the RNAi plants, indicating that transposons and cell death-related genes might be among the primary targets of OsSRT1-mediated gene silencing.<ref name="ref15" />.''

← Older revision		Revision as of 11:12, 5 June 2014
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	*We compared the expression of two hypersensitive response (HSR201 and HSR203J) marker genes <ref name="ref9" />.''and a cytochromeP450 gene (calledAPO) that is closely related to wheat (Triticum aestivum) CYP709C1 and CYP709C3v2, both of which are suggested to be involved in wheat defense to pathogens <ref name="ref10" />.''<ref name="ref11" />.''		*We compared the expression of two hypersensitive response (HSR201 and HSR203J) marker genes <ref name="ref9" />.''and a cytochromeP450 gene (calledAPO) that is closely related to wheat (Triticum aestivum) CYP709C1 and CYP709C3v2, both of which are suggested to be involved in wheat defense to pathogens <ref name="ref10" />.''<ref name="ref11" />.''
		+
		+	*Down-regulation of OsSRT1 by RNAi produces a lesion-mimic phenotype and induces H3K9 acetylation (H3K9ac) and expression of hypersensitive response-related genes in rice plants. In addition, the transcription of many transposable elements is activated in the RNAi plants, indicating
		+	that transposons and cell death-related genes might be among the primary targets of OsSRT1-mediated gene silencing.<ref name="ref15" />.''

← Older revision		Revision as of 11:09, 5 June 2014
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	<ref name="ref12"> Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J 11: 1187–1194</ref>		<ref name="ref12"> Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J 11: 1187–1194</ref>
		+
		+	<ref name="ref13">Servet C, Conde E, Silva N, Zhou D-X (2010) Histone acetyltransferase AtGCN5/HAG1 is a versatile regulator of developmental and inducible gene expression in Arabidopsis. Molecular plant 3: 670–677.</ref>
		+
		+	<ref name="ref14">Chen ZJ, Tian L (2007) Roles of dynamic and reversible histone acetylation in plant development and polyploidy. Biochimica et Biophysica Acta 1769: 295–307.</ref>
		+
	</references>		</references>

← Older revision		Revision as of 11:06, 5 June 2014
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	*OsSRT1 is a histone acetylation enzyme, participating in acetylation of H3K9, inhibition of transposon and gene expression related to cell apoptosis. Rice SIR2 gene is important to maintain genomic stability and avoid DNA damage in the process of plant cell growth. OsSRT1 is involved in the safeguard against genome instability and/or oxidative stress, required for plant cell growth<ref name="ref2" />.''		*OsSRT1 is a histone acetylation enzyme, participating in acetylation of H3K9, inhibition of transposon and gene expression related to cell apoptosis. Rice SIR2 gene is important to maintain genomic stability and avoid DNA damage in the process of plant cell growth. OsSRT1 is involved in the safeguard against genome instability and/or oxidative stress, required for plant cell growth<ref name="ref2" />.''
		+
		+
		+	*Histone lysine acetylation is an important epigenetic modification for genome function and gene activity. Dynamic modulation of histone acetylation in plants has been shown to be involved in gene expression programs of plant development and responses to environmental conditions <ref name="ref13" />.''<ref name="ref14" />.''

	===Expression===		===Expression===

← Older revision		Revision as of 08:49, 5 June 2014
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	<ref name="ref11"> Kong L, Anderson JM, OhmHW(2005) Induction of wheat defense and stressrelated genes in response to Fusarium graminearum. Genome 48: 29–40</ref>		<ref name="ref11"> Kong L, Anderson JM, OhmHW(2005) Induction of wheat defense and stressrelated genes in response to Fusarium graminearum. Genome 48: 29–40</ref>
		+
		+	<ref name="ref12"> Thordal-Christensen H, Zhang Z, Wei Y, Collinge DB (1997) Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley powdery mildew interaction. Plant J 11: 1187–1194</ref>
	</references>		</references>

← Older revision		Revision as of 08:46, 5 June 2014
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−	*The RNAi lines were selected for phenotype observation and further analysis. The RNAi plants at the two-leaf stage (about 14 d after germination) began to produce brown dots on leaves, which became larger at latter stages, leading to precocious leaf senescence. Only two of the three RNAi lines could produce seeds. The severest line died before getting into maturity. The transgenic lines showing no alteration of OsSRT1 expression or histonemodification did notmanifest the phenotype, suggesting that the lesion mimic phenotype was induced by OsSRT1 down-regulation. The lesions were reminiscent of cell death induced by hypersensitive responses during plant pathogen infections, suggesting that OsSRT1 RNAi	+	*The RNAi lines were selected for phenotype observation and further analysis. The RNAi plants at the two-leaf stage (about 14 d after germination) began to produce brown dots on leaves, which became larger at latter stages, leading to precocious leaf senescence. Only two of the three RNAi lines could produce seeds. The severest line died before getting into maturity. The transgenic lines showing no alteration of OsSRT1 expression or histonemodification did notmanifest the phenotype, suggesting that the lesion mimic phenotype was induced by OsSRT1 down-regulation. The lesions were reminiscent of cell death induced by hypersensitive responses during plant pathogen infections, suggesting that OsSRT1 RNAi might have induced programmed cell death (PCD). To test this hypothesis, young leaf sheaths (T1 generation) were incubated with 3,3#-diaminobenzidine (DAB) to detect H2O2 <ref name="ref12" />.''
−	might have induced programmed cell death (PCD). To test this hypothesis, young leaf sheaths (T1 generation) were incubated with 3,3#-diaminobenzidine (DAB) to detect H2O2 <ref name="ref12" />.''