Os04g0448500 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:04:02Z

‎Structured Information

2014-06-09T06:43:57Z

‎Expression

2014-06-09T06:38:50Z

‎Expression

2014-06-09T06:32:50Z

‎Expression

2014-06-09T06:25:33Z

‎Labs working on this gene

2014-06-09T06:25:14Z

‎Expression

2014-06-09T06:22:58Z

‎Expression

2014-06-09T05:30:26Z

‎References

2014-06-09T05:29:22Z

‎Labs working on this gene

2014-06-09T05:24:47Z

‎Annotated Information

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 [[File:QQ图片20140609141245.jpg]]
 After 4-h 20mM H2O2 treatment, 82% and 71% of the yeast cells overexpressing OsAP25 and OsAP37, respectively, displayed positive signals when labelled with the mammalian caspase substrate FIFC-VAD-fmk, suggesting that OsAP25and OsAP37 share a similar function with YCA1 in promoting PCD process in yeast.
 [[File:QQ图片20140609142222.jpg]]
 Expressed the full-length CDS of OsAP25 or OsAP37 driven by the CAMV 35S promoter in plants. Trypan blue vital staining of tobacco leaves revealed substantially increased death of epidermal and mesophyll cells in leaves transiently expressing OsAP25 and OsAP37 compared with those expressing GFP, or OsAP25 and OsAP37 together with the aspartic protease-specific inhibitor pepstatin A.
 ===Evolution===

← Older revision		Revision as of 06:38, 9 June 2014
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	After 4-h 20mM H2O2 treatment, 82% and 71% of the yeast cells overexpressing OsAP25 and OsAP37, respectively, displayed positive signals when labelled with the mammalian caspase substrate FIFC-VAD-fmk, suggesting that OsAP25and OsAP37 share a similar function with YCA1 in promoting PCD process in yeast.		After 4-h 20mM H2O2 treatment, 82% and 71% of the yeast cells overexpressing OsAP25 and OsAP37, respectively, displayed positive signals when labelled with the mammalian caspase substrate FIFC-VAD-fmk, suggesting that OsAP25and OsAP37 share a similar function with YCA1 in promoting PCD process in yeast.
		+
		+	[[File:QQ图片20140609142222.jpg]]
		+
		+	Expressed the full-length CDS of OsAP25 or OsAP37 driven by the CAMV 35S promoter in plants. Trypan blue vital staining of tobacco leaves revealed substantially increased death of epidermal and mesophyll cells in leaves transiently expressing OsAP25 and OsAP37 compared with those expressing GFP, or OsAP25 and OsAP37 together with the aspartic protease-specific inhibitor pepstatin A.

	===Evolution===		===Evolution===

← Older revision		Revision as of 06:32, 9 June 2014
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	[[File:QQ图片20140609141245.jpg]]		[[File:QQ图片20140609141245.jpg]]
		+
		+	After 4-h 20mM H2O2 treatment, 82% and 71% of the yeast cells overexpressing OsAP25 and OsAP37, respectively, displayed positive signals when labelled with the mammalian caspase substrate FIFC-VAD-fmk, suggesting that OsAP25and OsAP37 share a similar function with YCA1 in promoting PCD process in yeast.

	===Evolution===		===Evolution===

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 ==Labs working on this gene==
 . Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Hybrid Rice, Shanghai 200030, Peoples R China
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 . Univ Nottingham, Sch Biosci, Div Plant Sci, Loughborough, Leics, England
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 . Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA

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 ===Expression===
 Performed microarray analysis of ''eat1'' at stage 9 of anther development , and observed changes in th expression of many genes. SoAP37showed a significant reduction in expression, which was further confirmed by qRT-PCR.
 ===Evolution===

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 ==References==
-Please input cited references here.
+Ningning Niu;Wanqi Liang;Xijia Yang;Weilin Jin;Zoe A. Wilson;Jianping Hu;Dabing Zhang，''EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice''， Nature Communications, 2013, 4: 1445
 ==Structured Information==

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 ==Annotated Information==
 ===Function===
-Please input function information here.
+Programmed cell death is essential for the development of multicellular organisms, yet pathways of plant programmed cell death and its regulation remain elusive. And the OsAP37 and OsAP25, which encode aspartic proteases that induce programmed cell death in both yeast and plants, is regulted directly by ETERNAL TAPETUM1. That is to say ETERNAL TAPETUM 1 promotes aspartic proteases triggering plant programmed cell death, and reveals a dynamic regulatory cascade in male reproductive development in rice.
 ===Expression===