Os04g0473900 - Revision history

Xysj1980 at 02:52, 27 March 2017

2017-03-27T02:52:59Z

Xysj1980 at 02:52, 27 March 2017

2017-03-27T02:52:38Z

192.168.72.52: /* Structured Information */

2015-06-12T07:04:58Z

‎Structured Information

Mayamei0424: /* Mutation */

2014-06-01T01:56:43Z

‎Mutation

Mayamei0424: /* Expression */

2014-06-01T01:56:19Z

‎Expression

Mayamei0424: /* Mutation */

2014-06-01T01:51:53Z

‎Mutation

Mayamei0424: /* Mutation */

2014-06-01T01:50:52Z

‎Mutation

Mayamei0424: /* Evolution */

2014-06-01T01:49:13Z

‎Evolution

Mayamei0424: /* Evolution */

2014-06-01T01:48:31Z

‎Evolution

Mayamei0424: /* Expression */

2014-06-01T01:47:47Z

‎Expression

@@ Line 8: / Line 8: @@
 [[File:dwa1.jpg|right|thumb|250px|Fig.1 Screening of mutants(from reference<ref name="ref1" />)]]
 [[File:dwa2.jpg|right|thumb|250px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference<ref name="ref1" />)]]
-[[File:dwa3.jpg|right|thumb|250px|Fig.3 functional complementation assay analysis(from reference<ref name="ref1" />)]]
 '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''<br>
 ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).<br>

@@ Line 9: / Line 9: @@
 [[File:dwa2.jpg|right|thumb|250px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference<ref name="ref1" />)]]
 [[File:dwa3.jpg|right|thumb|250px|Fig.3 functional complementation assay analysis(from reference<ref name="ref1" />)]]
 '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''<br>
 ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).<br>
@@ Line 20: / Line 19: @@
 ===Expression===
 '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).<br>
 '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br>
@@ Line 30: / Line 27: @@
 ===Evolution===
 At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.
 ==Labs working on this gene==

@@ Line 43: / Line 43: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 6: / Line 6: @@
 ===Mutation===
-[[File:dwa1.jpg|right|thumb|200px|Fig.1 Screening of mutants(from reference<ref name="ref1" />)]]
+[[File:dwa1.jpg|right|thumb|250px|Fig.1 Screening of mutants(from reference<ref name="ref1" />)]]
-[[File:dwa2.jpg|right|thumb|200px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference<ref name="ref1" />)]]
+[[File:dwa2.jpg|right|thumb|250px|Fig.2 phenotypic analysis and the expression levels of wax-related rice genes in the ''dwa1'' mutant and WT(from reference<ref name="ref1" />)]]
-[[File:dwa3.jpg|right|thumb|200px|Fig.3 functional complementation assay analysis(from reference<ref name="ref1" />)]]
+[[File:dwa3.jpg|right|thumb|250px|Fig.3 functional complementation assay analysis(from reference<ref name="ref1" />)]]
-[[File:dwa4.jpg|right|thumb|200px|Fig.4 Overexpression of ''DWA1'' analysis(from reference<ref name="ref1" />)]]
+[[File:dwa4.jpg|right|thumb|250px|Fig.4 Overexpression of ''DWA1'' analysis(from reference<ref name="ref1" />)]]
 '''(1)''' '''Screening of mutants(Identification of the drought-hypersensitive ''dwa1'' mutant in rice.)'''<br>
 ''dwa1'' mutant is observed by inserting T-DNA in the sixth exon of ''DWA1''(Fig.1A and B).RT-qPCR analysis of ''DWA1'' in WT and mutant tissues(leaf and panicle) indicate that ''dwa1'' is a loss of-function mutant because of no transcript found in the mutant(Fig.1C and D).<br>

@@ Line 20: / Line 20: @@
 ===Expression===
-[[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]
+[[File:图片4.png|right|thumb|250px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]
-[[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference<ref name="ref1" />)]]
+[[File:图片5.png|right|thumb|250px|Fig.6 Drought-induced expression of ''DWA1''.(from reference<ref name="ref1" />)]]
 '''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).<br>
 '''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br>

@@ Line 29: / Line 29: @@
 ===Evolution===
-[[File:1(DWA1).png|right|thumb|200px|Fig.4 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]
+[[File:1(DWA1).png|center|thumb|200px|Fig.7 Unrooted phylogenetic tree and domain architecture of DWA1 protein orthologs in vascular plants and microbial relatives(from reference<ref name="ref1" />)]]
-At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.4).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.
+At present,DWA1,likely to a megaenzyme,has five domains at least. The N terminus of DWA1 is an oxidoreductase-like domain,and closely followed by the AMP-binding domain(A domain)Then,two repeats of a phosphopantetheine-binding subdomain follow the A domain and feature a thiolation domain (T domain).An allene oxide synthase (AOS)-like domain is located between the second and third repeats of the left-handed β-helix (LbH) domain at the C-terminal region<ref name="ref1"/>(Fig.7).What's more,homologous sequences with ''DWA1''are all from microorganisms.Nonribosomal peptide synthetase(NRPS),a megaenzyme needed for nonribosomal peptides synthesis in bacteria and filamentous fungi<ref name="ref2"/>,is a major branch of the AMP-binding enzyme superfamily.More importantly,both these homologous sequences and ''DWA1'' have an NRPS module, including adenylation domain, followed by a thiolation domain catalyzing the activation and thiolation reaction of an amino acid instead of a carboxyl substrate for acyl-CoA synthetase.These features suggest that DWA1 and its plant homologs may be derived from a prokaryotic NRPS rather than the acyl-CoA synthetase family, which possesses only the A domain<ref name="ref1"/>.
 ==Labs working on this gene==

@@ Line 20: / Line 20: @@
 ===Expression===
-[[File:图片4.png|right|thumb|200px|Fig.2 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]
+[[File:图片4.png|right|thumb|200px|Fig.5 Expression profiles of ''DWA1'' under normal and stress conditions.(from reference<ref name="ref1" />)]]
-[[File:图片5.png|right|thumb|200px|Fig.3 Drought-induced expression of ''DWA1''.(from reference<ref name="ref1" />)]]
+[[File:图片5.png|right|thumb|200px|Fig.6 Drought-induced expression of ''DWA1''.(from reference<ref name="ref1" />)]]
-'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.2A).<br>
+'''(1)'''qRCR results show that ''DWA1'' expression level is very low and undetectable at the vegetative stages but is relatively high at the reproductive stages(Fig.5A).<br>
-'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.2B 1-2), axillary bud (Fig.2B3), young panicle (Fig.2B4),spikelet (Fig.2B5), stem (Fig.2B6), young panicle (Fig.2B7), pistil (Fig.2B8), stamen (Fig.2B9), young embryo (Fig.2B10), SEM picture of rice leaf cuticle (Fig.2B11), cross-sections of stem (Fig.2B12) and leaf (Fig.2B13),and semithin sections of leaf (Fig.2B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br>
+'''(2)'''Using a GUS reporter gene driven by a ''DWA1'' promoter detects that ''DWA1'' expression is mainly in the mature organs, such as in mature leaves (Fig.5B1 and 2), axillary bud (Fig.5B3), young panicle (Fig.5B4),spikelet (Fig.5B5), stem (Fig.5B6), young panicle (Fig.5B7), pistil (Fig.5B8), stamen (Fig.5B9), young embryo (Fig.5B10), SEM picture of rice leaf cuticle (Fig.5B11), cross-sections of stem (Fig.5B12) and leaf (Fig.5B13),and semithin sections of leaf (Fig.5B14-15).In addition,SEM observation shows that the GUS staining in leaves is mainly distributed along the silica-cork cell lines in the cuticle.Cross-sections and semithin sections indicate that DWA1 is expressed mainly in vascular tissues and epidermal cell layers.<br>
 '''(3)'''What's more,after various phytohormones treatment,''DWA1'' expresses higher,such as abscisic acid, jasmonic acid, indole-3-acetic acid, and
-gibberellic acid (Fig.2C).<br>
+gibberellic acid (Fig.5C).<br>
-'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.3B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br>
+'''(4)'''Under drought treatment,GUS staining shows that''DWA1'' is induced.For example,in Fig.6B,GUS staining in leaf before (1 and 3) and after (2 and 4) drought stress is shown.<br>
 ===Evolution===