Os04g0509300 - Revision history

Xysj1988 at 17:40, 5 October 2016

2016-10-05T17:40:06Z

Xysj1988: /* Function */

2016-10-05T17:38:22Z

‎Function

Xysj1988: /* Evolution */

2016-10-05T17:37:57Z

‎Evolution

Xysj1988: /* Labs working on this gene */

2016-10-05T17:37:44Z

‎Labs working on this gene

Liman: /* Mutation */

2016-10-05T15:02:39Z

‎Mutation

Liman at 15:01, 5 October 2016

2016-10-05T15:01:52Z

Liman at 14:59, 5 October 2016

2016-10-05T14:59:17Z

Liman: /* References */

2016-10-05T14:57:33Z

‎References

Liman: /* Mutation */

2016-10-05T14:57:17Z

‎Mutation

Liman: /* Mutation */

2016-10-05T14:56:49Z

‎Mutation

← Older revision		Revision as of 17:40, 5 October 2016
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−	'''''~~Os DCL4~~''''' encodes DCL4 protein which is responsible for the processing of 21-nucleotide tasiRNAs that are required for normal plant development.	+	'''''OsDCL4''''' encodes DCL4 protein which is responsible for the processing of 21-nucleotide tasiRNAs that are required for normal plant development.

	==Annotated Information==		==Annotated Information==

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-* Biochemical and genetic analyses indicate that '''''Os DCL4''''' is the major Dicer responsible for the 21-nucleotide siRNAs associated with inverted repeat transgenes and for trans-acting siRNA (ta-siRNA) from the endogenous TRANS-ACTING siRNA3 (TAS3) gene.
+* Biochemical and genetic analyses indicate that '''''OsDCL4''''' is the major Dicer responsible for the 21-nucleotide siRNAs associated with inverted repeat transgenes and for trans-acting siRNA (ta-siRNA) from the endogenous TRANS-ACTING siRNA3 (TAS3) gene.
 ===Mutation===

← Older revision		Revision as of 17:37, 5 October 2016
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	Please input expression information here.		Please input expression information here.

−	~~===Evolution===~~
−	~~Please input evolution information here.~~

	You can also add sub-section(s) at will.		You can also add sub-section(s) at will.

@@ Line 28: / Line 28: @@
 ==Labs working on this gene==
-* State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental
+* State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
 * Graduate University of the Chinese Academy of Sciences, Beijing 100039, China
-* Laboratory of Molecular and Developmental Biology and National Center for Plant Gene Research, Institute of Genetics and
+* Laboratory of Molecular and Developmental Biology and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
 * National Institute of Biological Science, Beijing 102206, China
 * Department of Molecular, Cellular, and Development Biology, Yale University, New Haven, Connecticut 06520-8104

@@ Line 17: / Line 17: @@
 phenotypic progeny. 5S/tRNA stained with ethidium bromide was used as a control.''' '' <ref name="ref1" />.'']]
-* To determine the role of Os DCL4 encoded by Os 04g43050 in small RNA biogenesis and rice development, 15 individual transgenic plants expressing an inverted repeat of Os DCL4 (DCL4IR) were generated<ref name="ref2" />. During reproductive development, the basic branching architecture of the panicle in DCL4IR transformants was normal except for the absence of certain spikelets in the lower part of the rachis (Figures 1A and 1B). Compared with wild-type plants (Figure 1C), spikelet organ identity was greatly disrupted in the DCL4IR transformants (Figures 1D to 1F). Three typical phenotypes from weak to strong were observed, including a slight opening between the lemma and the palea (Figure 1D) and the lemma being partially or completely degenerated to the awn (Figures 1E and 1F). Moreover, the empty glumes and the rudimentary glumes were separated in DCL4IR transformants (Figures 1D to 1F) instead of being close together, as in the wild-type control (Figure 1C). As a result, the strong loss-of-function transgenic plants (Figure 1F) were sterile and only two weak loss-of-function plants could produce seeds for further genetic analysis (Figure 1D). The offspring from the weak loss-of-function plants (Figure 1D) showed the expected 1:3 segregation of normal to abnormal phenotype. When 41 T1 plants from two individual transgenic lines were examined, the abnormal phenotype cosegregated with the DCL4IR transgenes (Figure 1G).
+* To determine the role of Os DCL4 protein in small RNA biogenesis and rice development, 15 individual transgenic plants expressing an inverted repeat of Os DCL4 (DCL4IR) were generated<ref name="ref2" />. During reproductive development, the basic branching architecture of the panicle in DCL4IR transformants was normal except for the absence of certain spikelets in the lower part of the rachis (Figures 1A and 1B). Compared with wild-type plants (Figure 1C), spikelet organ identity was greatly disrupted in the DCL4IR transformants (Figures 1D to 1F). Three typical phenotypes from weak to strong were observed, including a slight opening between the lemma and the palea (Figure 1D) and the lemma being partially or completely degenerated to the awn (Figures 1E and 1F). Moreover, the empty glumes and the rudimentary glumes were separated in DCL4IR transformants (Figures 1D to 1F) instead of being close together, as in the wild-type control (Figure 1C). As a result, the strong loss-of-function transgenic plants (Figure 1F) were sterile and only two weak loss-of-function plants could produce seeds for further genetic analysis (Figure 1D). The offspring from the weak loss-of-function plants (Figure 1D) showed the expected 1:3 segregation of normal to abnormal phenotype. When 41 T1 plants from two individual transgenic lines were examined, the abnormal phenotype cosegregated with the DCL4IR transgenes (Figure 1G).
 ===Expression===

@@ Line 1: / Line 1: @@
-The rice gene Os 04g43050 was reported as '''''Os DCL4''''' in 2007<ref name="ref1" />.
+'''''Os DCL4''''' encodes DCL4 protein which is responsible for the processing of 21-nucleotide tasiRNAs that are required for normal plant development.
 ==Annotated Information==
 ===Function===

@@ Line 47: / Line 47: @@
 developmental defects in rice. Plant Physiol. 139: 296–305.
 </ref>
 </references>

@@ Line 6: / Line 6: @@
 ===Mutation===
 [[File:Os04g0509300-1.png|right|thumb|400px|'''Figure 1. Knockdown of Os DCL4 Causes Abnormal Morphology in Rice.
@@ Line 17: / Line 16: @@
 and line 2 are two independent T1 generation lines of DCL4IR transformants. AF, transgenic plants displaying abnormal flowering; WTF, wild-type
 phenotypic progeny. 5S/tRNA stained with ethidium bromide was used as a control.''' '' <ref name="ref1" />.'']]
 ===Expression===

@@ Line 8: / Line 8: @@
 * To determine the role of Os DCL4 encoded by Os 04g43050 in small RNA biogenesis and rice development, 15 individual transgenic plants expressing an inverted repeat of Os DCL4 (DCL4IR) were generated<ref name="ref2" />. During reproductive development, the basic branching architecture of the panicle in DCL4IR transformants was normal except for the absence of certain spikelets in the lower part of the rachis (Figures 1A and 1B). Compared with wild-type plants (Figure 1C), spikelet organ identity was greatly disrupted in the DCL4IR transformants (Figures 1D to 1F). Three typical phenotypes from weak to strong were observed, including a slight opening between the lemma and the palea (Figure 1D) and the lemma being partially or completely degenerated to the awn (Figures 1E and 1F). Moreover, the empty glumes and the rudimentary glumes were separated in DCL4IR transformants (Figures 1D to 1F) instead of being close together, as in the wild-type control (Figure 1C). As a result, the strong loss-of-function transgenic plants (Figure 1F) were sterile and only two weak loss-of-function plants could produce seeds for further genetic analysis (Figure 1D). The offspring from the weak loss-of-function plants (Figure 1D) showed the expected 1:3 segregation of normal to abnormal phenotype. When 41 T1 plants from two individual transgenic lines were examined, the abnormal phenotype cosegregated with the DCL4IR transgenes (Figure 1G).
-[[File:Os04g0509300-1.png|center|thumb|400px|'''Figure 1. Knockdown of Os DCL4 Causes Abnormal Morphology in Rice.
+[[File:Os04g0509300-1.png|right|thumb|400px|'''Figure 1. Knockdown of Os DCL4 Causes Abnormal Morphology in Rice.
 (A) An inflorescence of wild-type Nipponbare rice.
 (B) An inflorescence of DCL4IR transgenic rice.