Os04g0509600 - Revision history

Rice999 at 14:33, 13 October 2016

2016-10-13T14:33:47Z

Rice999 at 14:32, 13 October 2016

2016-10-13T14:32:42Z

Rice999 at 14:29, 13 October 2016

2016-10-13T14:29:12Z

192.168.72.52: /* Structured Information */

2015-06-12T07:07:35Z

‎Structured Information

Remedychen: /* References */

2014-06-10T02:58:21Z

‎References

Remedychen: /* References */

2014-06-10T02:57:27Z

‎References

Remedychen: /* References */

2014-06-10T02:36:38Z

‎References

Remedychen: /* Evolution */

2014-06-10T02:21:54Z

‎Evolution

Remedychen: /* Expression */

2014-06-10T02:21:27Z

‎Expression

Remedychen: /* Function */

2014-06-10T02:20:32Z

‎Function

@@ Line 2: / Line 2: @@
 ==Annotated Information==
-[[File:62-Os04g0509600-1.png|right|thumb|327px|'''Figure 1.''' ''The relative expression of the OsAMT1;1 gene and plant growth parameters of transgenic and wild-type plants grown in hydroponics.<ref name="ref1" />.'']]
+[[File:62-Os04g0509600-1.png|right|thumb|427px|'''Figure 1.''' ''The relative expression of the OsAMT1;1 gene and plant growth parameters of transgenic and wild-type plants grown in hydroponics.<ref name="ref1" />.'']]
 *'''''Os04g0509600''''' '''''<=>''''' '''''OsAMT1;1''''','''''OsAMT1''''','''''AMT1-1''''','''''AMT1''''','''''OsAMT1-1'''''
 ===Function===
@@ Line 21: / Line 21: @@
 * In general, plants grown in 3000 μM exhibited the highest expression levels, whereas plants in 30 μM exhibited the lowest expression.
 * The expression of plants at 300  μM was intermediate. This indicates that OsAMT1;1 is an NH 4+ -responsive gene. Analysis of relative '''''OsAMT1;1''''' gene expression throughout the day showed that its expression was greatest from morning to noon, but decreased significantly in the late afternoon and evening. This was an ~8-fold reduction compared with its maximum value at noon.
+[[File:62-Os04g0509600-2.png|right|thumb|427px|'''Figure 3.''' ''Intercellular localization of OsAMT1;1 protein.<ref name="ref1" />.'']]
-===Evolution===
+===Subcellular localization===
 * The intercellular localization of the OsAMT1;1 gene was investigated in onion epidermal cells expressing N-terminal GFP::OsAMT1;1 fusions under the control of the 35S promoter from Cauliflower mosaic virus (CaMV).
 * Successfully transformed cells exhibited RFP fused to the plasma membrane in the onion epidermis (Fig. 3A). GFP-dependent fluorescence was also observed in the plasma membrane of onion epidermal cells (Fig.  3B).
 * The merged image (Fig.  3C) clearly showed co-localization of RFP and GFP in the plasma membrane; Fig. 3D shows a light micrograph of the transformed onion epidermal cell. These results indicated that the OsAMT1;1 transporter protein is localized in the plasma membrane.
-[[File:62-Os04g0509600-2.png|right|thumb|327px|'''Figure 3.''' ''Intercellular localization of OsAMT1;1 protein.<ref name="ref1" />.'']]
 ==Labs working on this gene==
 * Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1

@@ Line 2: / Line 2: @@
 ==Annotated Information==
 *'''''Os04g0509600''''' '''''<=>''''' '''''OsAMT1;1''''','''''OsAMT1''''','''''AMT1-1''''','''''AMT1''''','''''OsAMT1-1'''''
 ===Function===
@@ Line 25: / Line 26: @@
 * Successfully transformed cells exhibited RFP fused to the plasma membrane in the onion epidermis (Fig. 3A). GFP-dependent fluorescence was also observed in the plasma membrane of onion epidermal cells (Fig.  3B).
 * The merged image (Fig.  3C) clearly showed co-localization of RFP and GFP in the plasma membrane; Fig. 3D shows a light micrograph of the transformed onion epidermal cell. These results indicated that the OsAMT1;1 transporter protein is localized in the plasma membrane.
+[[File:62-Os04g0509600-2.png|right|thumb|327px|'''Figure 3.''' ''Intercellular localization of OsAMT1;1 protein.<ref name="ref1" />.'']]
 ==Labs working on this gene==
 * Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os04g0509600''''' was reported as '''''OsAMT1;1''''' in 2013 <ref name="ref1" /> by researchers from Canada.
 ==Annotated Information==
 ===Function===
-OsAMT1;1（Os04g0509600）is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3).OsAMT1;1 has ammonium transporters activity.The amino acid sequences of the OsAMT1;1, OsAMT1;2 and OsAMT1;3 share high sequence similarity to each other and to other AMT1-type ammonium transporters previously identified from other plant species<ref name="ref1" />.All three isolated OsAMT1 genes encode functional ammonium transporters.OsAMT1;1 responds to ammonium in a comparable time and concentration-dependent manner,although absolute changes in transcript accumulation were less pronounced.The first step in nitrogen assimilation by plants is the uptake of the nitrate and ammonium into root cells from the soil solution. Since all three isolated transporter genes werefunctional in yeast, their translation products are thought to primarily contribute to the uptake of ammonium into the root.Because exclusive supply of ammonium is harmful, the ammonium taken up by the OsAMT1s is probably assimilated into glutamine through the reaction catalyzed by glutamine synthetase (GS) via glutamate through the reaction by glutamate synthase (GOGAT), designated as the concerted GS/GOGAT cycle<ref name="ref3" />.
+* '''''OsAMT1;1''''' is one of the OsAMT1 family members which contain three genes(OsAMT1;1,OsAMT1;2 and OsAMT1;3).
 ===Expression===
-OsAMT1;1 expression was constitutive in shoots and promoted by ammonium in roots;OsAMT1;2 expression was root specific and ammonium inducible,while OsAMT1;3 was also expressed specifically in roots but was repressed by nitrogen. These results implied feedback regulation, i.e. positive and negative control mechanisms for AMT1 genes in rice.It has been suggested that the cellular nitrogen status controls nitrogen and carbon metabolism as well as nitrogen absorption in roots<ref name="ref1" /><ref name="ref2" />.
+* For comparison, the expression of the '''''OsAMT1''''';1 gene in WT plants grown in 300 μM NH 4+ (optimum concentration) was set as the base value of 1 in both roots and shoots. The relative expression of the OsAMT1;1 gene in transgenic lines was ~10- to 30-fold the expression in the WT, in both roots and shoots.
 ===Evolution===
-   Diversity and selective sweep in the OsAMT1;1 genomic region of rice.
+* The intercellular localization of the OsAMT1;1 gene was investigated in onion epidermal cells expressing N-terminal GFP::OsAMT1;1 fusions under the control of the 35S promoter from Cauliflower mosaic virus (CaMV).
-   Effects of the fungal endophyte Phomopsis liquidambari on nitrogen uptake and metabolism in rice.
+* Successfully transformed cells exhibited RFP fused to the plasma membrane in the onion epidermis (Fig. 3A). GFP-dependent fluorescence was also observed in the plasma membrane of onion epidermal cells (Fig.  3B).
-   Influence of different nitrogen inputs on the members of ammonium transporter and glutamine synthetase genes in two rice genotypes having differential responsiveness to nitrogen.
+* The merged image (Fig.  3C) clearly showed co-localization of RFP and GFP in the plasma membrane; Fig. 3D shows a light micrograph of the transformed onion epidermal cell. These results indicated that the OsAMT1;1 transporter protein is localized in the plasma membrane.
 ==Labs working on this gene==
-Please input related labs here.
+* Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada, N1G 2W1
 ==References==
 <references>
-<ref name="ref1">Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.</ref>
+<ref name="ref1">Ranathunge K, El-Kereamy A, Gidda S, Bi YM, Rothstein SJ. AMT1;1 transgenic
-<ref name="ref2">Sonoda Y, Ikeda A, Saiki S, et al. Distinct expression and function of three ammonium transporter genes (OsAMT1; 1–1; 3) in rice[J]. Plant and Cell Physiology, 2003, 44(7): 726-734.</ref>
+rice plants with enhanced NH4(+) permeability show superior growth and higher
-<ref name="ref3">Suenaga A, Moriya K, Sonoda Y, et al. Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants[J]. Plant and Cell Physiology, 2003, 44(2): 206-211.</ref>
+yield under optimal and suboptimal NH4(+) conditions. J Exp Bot. 2014
 </references>

@@ Line 25: / Line 25: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 18: / Line 18: @@
 ==References==
 <ref name="ref1">Sonoda Y, Ikeda A, Yamaya T, et al. Feedback regulation of the ammonium transporter gene family AMT1 by glutamine in rice[C]//Plant and Cell Physiology. GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND: OXFORD UNIV PRESS, 2004, 45: S98-S98.</ref>
 <ref name="ref2">Sonoda Y, Ikeda A, Saiki S, et al. Distinct expression and function of three ammonium transporter genes (OsAMT1; 1–1; 3) in rice[J]. Plant and Cell Physiology, 2003, 44(7): 726-734.</ref>