Os04g0541700 - Revision history

Xysj1981 at 08:18, 9 March 2017

2017-03-09T08:18:07Z

Xysj1981 at 08:17, 9 March 2017

2017-03-09T08:17:41Z

Liujia: /* Labs working on this gene */

2014-06-08T08:10:03Z

‎Labs working on this gene

Liujia: /* Function */

2014-06-07T15:04:56Z

‎Function

Liujia: /* Annotated Information */

2014-06-07T15:00:33Z

‎Annotated Information

Liujia: /* Function */

2014-06-07T15:00:08Z

‎Function

Liujia: /* Expression */

2014-06-07T14:59:20Z

‎Expression

Liujia: /* Function */

2014-06-07T14:58:33Z

‎Function

Liujia: /* Function */

2014-06-07T14:57:36Z

‎Function

Liujia: /* Function */

2014-06-07T14:56:20Z

‎Function

← Older revision		Revision as of 08:18, 9 March 2017
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	Gene Os04g0541700,namely '''Oshox22''',means Homeobox-leucine zipper protein HOX22.		Gene Os04g0541700,namely '''Oshox22''',means Homeobox-leucine zipper protein HOX22.
−
−	~~[mailto:huanghuasheng@xtbg.ac.cn email me]~~

	===Function===		===Function===

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 ===Evolution===
-Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors. The HD-Zip genes, are an abundant group of transcription factors that are exclusively found in plants<ref name="ref 1"/>. The NJ tree (Fig. 5) shows that the HD-Zip genes of family I can be divided into nine clades  as ''α'', ''β''1, ''β''2, ''γ'', ''δ'', ''ε'', ''ζ'', ''φ''1 and ''φ''2<ref name="ref 2"/>. Subfamilies IB, IC and ID all have an intron at the same position in the Zip region. ''Oshox22'' belongs to subfamily IB.[[File:HD-ZIP tree.jpg|thumbnail|'''Fig. 5''' Family I is subdivided in clades ''α'', ''β''1, ''β''2, ''γ'', ''δ'', ''ε'', ''ζ'', ''φ''1 and ''φ''2<ref name="ref 2"/>. In the picture,after each gene, the intron subfamily is indicated with a box (family I), circle (family II) and triangle (family III), respectively, in different grey tones or patterns. Below: Subfamily division based on exon-intron organization. The exon-intron pattern is divided into seven subfamilies for family I (IA, IB, IC, ID, IE, IF and IG), and both family II (IIA, IIB, IIC and IID) and III (IIIA, IIIB, IIIC and IIID) have four subfamilies. Open and filled boxes represent coding and conserved domains, respectively. The HD, Zip and START domains are indicated in light grey, dark grey and black, respectively. The position of the introns is shown with black arrowheads. The white arrowhead and star (*) refers to an alternative transcript occurring with Oshox6 in subfamily IB]]
+Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors. The HD-Zip genes, are an abundant group of transcription factors that are exclusively found in plants<ref name="ref 1"/>. The NJ tree (Fig. 5) shows that the HD-Zip genes of family I can be divided into nine clades  as ''α'', ''β''1, ''β''2, ''γ'', ''δ'', ''ε'', ''ζ'', ''φ''1 and ''φ''2<ref name="ref 2"/>. Subfamilies IB, IC and ID all have an intron at the same position in the Zip region. ''Oshox22'' belongs to subfamily IB.
 ==Labs working on this gene==
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 </ref>
 </references>

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 ==Labs working on this gene==
-Please input related labs here.
+*S. Zhang, L. Jiang, C.-M. Liu, P. B. F. Ouwerkerk: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. e-mail: p.b.f.ouwerkerk.2@gmail.com<br>
 ==References==

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 * Transient expression assays were carried out with effector and reporter plasmids in rice protoplasts to confirm the interaction between Oshox22 protein and pseudopalindromic AH1 (CAAT(A/T)ATTG) and AH2 (CAAT(C/G)ATTG) sequences. Two reporter plasmids, ''4AH1-90-GUS'' and ''4AH2-90-GUS''<ref name="ref 2"/> <ref name="ref 3"/> were used, in which the AH1 and AH2 tetramers were fused to a CaMV -90 35S minimal promoter. Construct ''Pro35SOshox22'' with ''Oshox22'' expressed under control of the CaMV 35S promoter was used as an effector. The data show in Fig. 2 indicate that Oshox22 is capable to activate transcription of the reporter gene when upstream HD-Zip binding sites AH1 or AH2 are present. The interaction is less effective at the AH1 site than that at AH2<ref name="ref 1"/>.<br><br>
 * Oshox22 functions in regulating ABA biosynthesis and ABA-mediated drought and salt tolerances in rice. The differences in ABA levels are likely causing changes in sensitivity of germinating and developing seedlings towards exogenous applied ABA<ref name="ref 1"/>.
 ===Expression===

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 [mailto:huanghuasheng@xtbg.ac.cn email me]
-==Annotated Information==
+===Function===
-===Function===[[File:Oshox22  structure.jpg|thumbnail|'''Fig. 1''' sequence annotation of ''Oshox22''.]][[File:Oshox22 binding.jpg|thumbnail|'''Fig. 2''' Interactions of Oshox22 with the HD-Zip binding site AH2 (CAAT(C/G)ATTG) and activation of reporter gene expression in a transient expression system using rice protoplasts <ref name="ref 1"/> .]]
+[[File:Oshox22  structure.jpg|thumbnail|'''Fig. 1''' sequence annotation of ''Oshox22''.]][[File:Oshox22 binding.jpg|thumbnail|'''Fig. 2''' Interactions of Oshox22 with the HD-Zip binding site AH2 (CAAT(C/G)ATTG) and activation of reporter gene expression in a transient expression system using rice protoplasts <ref name="ref 1"/> .]]
 * ''Oshox22'' (Os04g0541700) is located on chromosome 4 and encodes a protein of 262 amino acids (AA) including a 61-AA HD domain for DNA binding and a 43-AA Zip domain for protein–protein interactions<ref name="ref 1"/>(Fig. 1).<br><br>
 * Oshox22 is a nuclear-localized protein. Trans-activation assays in yeast and transient expression analyses in rice protoplasts demonstrated that Oshox22 is able to bind the CAAT(G/C)ATTG element and acts as a transcriptional activator that requires both the HD and Zip domains<ref name="ref 1"/>. This is typical of HD-Zip I family transcription factors<ref name="ref 2"/>.<br><br>

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 ===Expression===
-* Expressed in seedlings, roots, stems, leaf sheaths and blades and panicles.<br><br>
+* Expressed in seedlings, roots, stems, leaf sheaths and blades and panicles.<br><br>[[File:Oshox22 expression.jpg|thumbnail|'''Fig. 3''' Expression profiling of ''Oshox22''<ref name="ref 1"/> .]]
 * Oshox22 is demonstrated as a nuclear-localized protein<ref name="ref 1"/>.<br><br>
 * Zhang S et al.<ref name="ref 1"/> used quantitative RT-PCR(qRT-PCR) to analyse RNA samples from different rice tissues at several developmental stages. The results showed that ''Oshox22'' is ubiquitously expressed, with a lower level in stems and higher in panicles and seeds(Fig. 3a).<br><br>
-* Agalou et al.<ref name="ref 4"/> demonstrated that the expression of ''Oshox22'' in rice is strongly induced by drought, and that the induction is higher in three drought tolerant upland cultivars compared with three lowland cultivars. Zhang S et al.<ref name="ref 1"/> monitored the ''Oshox22'' expression under different abiotic stress conditions. As shown in Fig. 3b, ''Oshox22'' was rapidly and strongly induced by NaCl, PEG and ABA, and weakly induced by low temperature<ref name="ref 1"/> .[[File:Oshox22 expression.jpg|thumbnail|'''Fig. 3''' Expression profiling of ''Oshox22''<ref name="ref 1"/> .]]
+* Agalou et al.<ref name="ref 4"/> demonstrated that the expression of ''Oshox22'' in rice is strongly induced by drought, and that the induction is higher in three drought tolerant upland cultivars compared with three lowland cultivars. Zhang S et al.<ref name="ref 1"/> monitored the ''Oshox22'' expression under different abiotic stress conditions. As shown in Fig. 3b, ''Oshox22'' was rapidly and strongly induced by NaCl, PEG and ABA, and weakly induced by low temperature<ref name="ref 1"/> .
 ===Evolution===