Os04g0599300 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:11:21Z

‎Structured Information

Suchao13: /* Expression */

2014-05-23T15:50:54Z

‎Expression

Suchao13: /* Expression */

2014-05-23T15:48:17Z

‎Expression

Suchao13: /* Evolution */

2014-05-23T15:44:31Z

‎Evolution

Suchao13: /* Mutation */

2014-05-23T15:40:41Z

‎Mutation

Suchao13: /* Mutation */

2014-05-06T01:52:52Z

‎Mutation

Zhangzhang at 01:51, 6 May 2014

2014-05-06T01:51:06Z

Suchao13: /* References */

2014-05-06T01:45:05Z

‎References

Suchao13: /* Knowledge Extension */

2014-05-06T01:42:20Z

‎Knowledge Extension

Suchao13: /* References */

2014-05-06T01:37:45Z

‎References

@@ Line 76: / Line 76: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 15:50, 23 May 2014
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	[[File:s6.jpg]]		[[File:s6.jpg]]
		+
		+	OsAP25 and OsAP37 can promote cell death.
		+
		+	[[File:s8.jpg]]

	===Evolution===		===Evolution===

@@ Line 26: / Line 26: @@
 ===Expression===
-The result of qRT–PCR indicated that in the wild type, EAT1 is weakly expressed in roots, shoots and leaves, and highly expressed in the anther from stage 7 to 12, while a dramatic reduction in expression was detected in the anthers in all three eat1 alleles (Fig. 4a). In GUS stained EAT1pro:GUS transgenic flowers, GUS signals started to appear in anthers at stage 7, became stronger from stage 8 to 9, and were nearly undetectable at stage 12 (Fig. 4b). Further in situ RNA hybridization indicated that EAT1 is highly expressed in the tapetum (Fig. 4c).[[File:图4.jpg]]
+The result of qRT–PCR indicated that in the wild type, EAT1 is weakly expressed in roots, shoots and leaves, and highly expressed in the anther from stage 7 to 12, while a dramatic reduction in expression was detected in the anthers in all three eat1 alleles. In GUS stained EAT1pro:GUS transgenic flowers, GUS signals started to appear in anthers at stage 7, became stronger from stage 8 to 9, and were nearly undetectable at stage 12. Further in situ RNA hybridization indicated that EAT1 is highly expressed in the tapetum.
 ===Evolution===

@@ Line 29: / Line 29: @@
 ===Evolution===
-Using the full-length EAT1 protein sequence to search available public databases and retrieved a total of 26 homologues from 10 diverse plant species from moss, pteridophytes, to angiosperms. The result show that EAT1 and three homologues from Sorghum bicolour (Sb04g030850), Zea mays (ZmLOC100282922) and Brachypodium distachyon (BradXP_003580474), respectively, were grouped in a subclade. EAT1 has one homologue from rice (OsbHLH142), which shares 40.8% identity with EAT1 in the HLH and DUF domains, and three homologues from Arabidopsis (AtbHLH091, AtbHLH089, AtbHLH010), which share an average of B40% identity with EAT1 in these two conserved domains.
+Using the full-length EAT1 protein sequence to search available public databases and retrieved a total of 26 homologues from 10 diverse plant species from moss, pteridophytes, to angiosperms. Phylogenetic analysis showed that EAT1 and the 26 homologues each have a single HLH domain and a conserved but
-[[File:图5.jpg]]
+[[File:s7.jpg]]
 ===Knowledge Extension===

@@ Line 11: / Line 11: @@
 ===Mutation===
-There are three kinds of EAT1 Mutation show in figure 2. The mutant exhibits normal vegetative development and female organ formation, but is completely male sterile and has shrunken anthers and aborted pollen grains (figure 3).
+There are three kinds of EAT1 Mutation.
-[[File:图2.jpg]][[File:图3.jpg]]
+[[File:s2.jpg]]
-The ''eat1-1'' mutant anthers appeared to undergo normal meiosis, forming tetrads of haploid
+The mutant exhibits normal vegetative development and female organ formation, but is completely male sterile and has shrunken anthers and aborted pollen grains . All F1 plants of reciprocal crosses between the wild type and the mutant were fertile, and the F2 plants had an approximate 3:1 ratio for phenotypic segregation (fertility: sterility¼160: 47, w2¼0.58, P40.05, w2 test used), suggesting that this male sterile phenotype is caused by a single recessive mutation
-microspores at late stage 8. But after stage 10, the ''eat1-1'' mutant had thicker tapetal cells, and abnormal abortion of the anther locule microspores. The result of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay and transmission electron microscopy (TEM) suggesting ''eat1-1'' mutant cause delayed and abnormal PCD in tapetal cell.
+Similar to the wild type, the eat1-1 mutant anthers appeared to undergo normal meiosis, forming tetrads of haploid microspores at late stage 8 (stage 8b) Consistent with this observation, normal chromosome separation during meiosis and regular tetrads could be seen in eat1-1 microspore mother cells, as revealed by 40,6-diamidino-2-phenylindole staining. After stage 10, the wild-type tapetal cells had condensed cytoplasm, then became thinner and were eventually degenerated before mature pollen grains were formed. In contrast, the eat1-1 mutant had thicker and darkly stained tapetal cells, and abnormal abortion of the anther locule and microspores. These results together show that in the eat1 mutant, anther development and microspore formation are aborted.The TUNEL-positive signal in wild-type tapetal cells commenced at stage 8 during late meiosis, became intense at stage 9 when the microspore is released from the tetrad, and weakened at stage 12 when mature pollen grains are formed. However, no obvious DNA fragmentation signal was observed in eat1 tapetal cells till stage 10, when the vacuolated microspore forms. At stage 11 during mitosis I, weak DNA fragmentation signal was seen in all four layers of the eat1 anthers, suggesting delayed and abnormal PCD.
 ===Expression===

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
 ETERNAL TAPETUM 1(EAT1), a basic helix-loop-helix transcription factor conserved in land
 plants, positively regulates programmed cell death in tapetal cells in rice anthers. eat1 exhibits
@@ Line 12: / Line 11: @@
 ===Mutation===
 There are three kinds of EAT1 Mutation show in figure 2. The mutant exhibits normal vegetative development and female organ formation, but is completely male sterile and has shrunken anthers and aborted pollen grains (figure 3).[[File:图2.jpg]][[File:图3.jpg]]
@@ Line 19: / Line 17: @@
 ===Expression===
 The result of qRT–PCR indicated that in the wild type, EAT1 is weakly expressed in roots, shoots and leaves, and highly expressed in the anther from stage 7 to 12, while a dramatic reduction in expression was detected in the anthers in all three eat1 alleles (Fig. 4a). In GUS stained EAT1pro:GUS transgenic flowers, GUS signals started to appear in anthers at stage 7, became stronger from stage 8 to 9, and were nearly undetectable at stage 12 (Fig. 4b). Further in situ RNA hybridization indicated that EAT1 is highly expressed in the tapetum (Fig. 4c).[[File:图4.jpg]]
 ===Evolution===
 Using the full-length EAT1 protein sequence to search available public databases and retrieved a total of 26 homologues from 10 diverse plant species from moss, pteridophytes, to angiosperms. The result show that EAT1 and three homologues from Sorghum bicolour (Sb04g030850), Zea mays (ZmLOC100282922) and Brachypodium distachyon (BradXP_003580474), respectively, were grouped in a subclade. EAT1 has one homologue from rice (OsbHLH142), which shares 40.8% identity with EAT1 in the HLH and DUF domains, and three homologues from Arabidopsis (AtbHLH091, AtbHLH089, AtbHLH010), which share an average of B40% identity with EAT1 in these two conserved domains.
@@ Line 32: / Line 25: @@
 ===Knowledge Extension===
 Tapetal cells is the innermost anther wall layer. After the meiosis of microspore mother cells it enter into programmed cell death (PCD).  This degradation process is essential for microspore development and provide materials for pollen wall maturation; as a result, premature or delayed tapetal degradation causes male sterility.
 In rice Several transcriptional regulators have been reported to be associated with tapetal degeneration. GAMYB[3], UNDEVELOPED TAPETUM1[4], TAPETUM DEGENERATION RETARDATION(TDR)[2], PERSISTENT TAPETAL CELL 1 (PTC1)[5], and APOPTOSIS INHIBITOR5 (API5)[6], ETERNAL TAPETUM 1(EAT1)[1]. TDR, the rice ortholog of the Arabidopsis AMS protein[2]. PTC，the rice ortholog of the Arabidopsis MS1 protein[5]. EAT1 shares sequence similarity with AtbHLH089 and AtbHLH091[1,7].
@@ Line 40: / Line 32: @@
 ==Labs working on this gene==
-Please input related labs here.
+*State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
+*Division of Plant Sciences,School of Biosciences, University of Nottingham, Loughborough, Leics, UK
-State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
+*Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA.
 ==References==
 [1] Ningning Niu. ''et a''l. EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice. NATURE COMMUNICATIONS. 4:1445. (2013). DOI: 10.1038/ncomms2396

@@ Line 51: / Line 51: @@
 [1] Ningning Niu. ''et a''l. EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice. NATURE COMMUNICATIONS. 4:1445. (2013). DOI: 10.1038/ncomms2396
-[2] Hui Li. et al. PERSISTENT TAPETAL CELL1 encodes a PHD-finger protein that is required for tapetal cell death and pollen development in rice. Plant Physiol.
+[2] Hui Li. ''et al.'' PERSISTENT TAPETAL CELL1 encodes a PHD-finger protein that is required for tapetal cell death and pollen development in rice. Plant Physiol.
 , 615–630 (2011).
-[3] Aya, K. et al. Gibberellin modulates anther development in rice via the transcriptional regulation of GAMYB. Plant cell 21 1453–1472 (2009).
+[3] Aya, K. ''et al''. Gibberellin modulates anther development in rice via the transcriptional regulation of GAMYB. Plant cell 21 1453–1472 (2009).
 [4] Jung, K. H. et al. Rice Undeveloped Tapetum1 is a major regulator of early tapetum development. Plant Cell 10, 2705–2222 (2005).
-[5] Li, H. et al. PERSISTENT TAPETAL CELL1 encodes a PHD-finger protein that is required for tapetal cell death and pollen development in rice. Plant Physiol.
+[5] Li, H. ''et al''. PERSISTENT TAPETAL CELL1 encodes a PHD-finger protein that is required for tapetal cell death and pollen development in rice. Plant Physiol.
 , 615–630 (2011).
-[6] Li, X. W. et al. Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5’-triphosphate-dependent RNA helicases regulates tapetum degeneration. Plant Cell 23, 1416–1434 (2011).
+[6] Li, X. W. ''et al.'' Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5’-triphosphate-dependent RNA helicases regulates tapetum degeneration. Plant Cell 23, 1416–1434 (2011).
-[7] Li, X. X. et al. Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis. Plant Physiol. 141, 1167–1184 (2006).
+[7] Li, X. X. ''et al''. Genome-wide analysis of basic/helix-loop-helix transcription factor family in rice and Arabidopsis. Plant Physiol. 141, 1167–1184 (2006).
 ==Structured Information==

@@ Line 34: / Line 34: @@
 Please input information here.
 Tapetal cells is the innermost anther wall layer. After the meiosis of microspore mother cells it enter into programmed cell death (PCD).  This degradation process is essential for microspore development and provide materials for pollen wall maturation; as a result, premature or delayed tapetal degradation causes male sterility.
-In rice Several transcriptional regulators have been reported to be associated with tapetal degeneration. GAMYB[3], UNDEVELOPED TAPETUM1[4], TAPETUM DEGENERATION RETARDATION(TDR)[2], PERSISTENT TAPETAL CELL 1 (PTC1)[5], and APOPTOSIS INHIBITOR5 (API5)[6], ETERNAL TAPETUM 1(EAT1)[1]. TDR, the rice ortholog of the Arabidopsis AMS protein[2]. PTC，the rice ortholog of the Arabidopsis MS1 protein[5]. EAT1 shares sequence similarity with AtbHLH089 and AtbHLH091[1,6].
+In rice Several transcriptional regulators have been reported to be associated with tapetal degeneration. GAMYB[3], UNDEVELOPED TAPETUM1[4], TAPETUM DEGENERATION RETARDATION(TDR)[2], PERSISTENT TAPETAL CELL 1 (PTC1)[5], and APOPTOSIS INHIBITOR5 (API5)[6], ETERNAL TAPETUM 1(EAT1)[1]. TDR, the rice ortholog of the Arabidopsis AMS protein[2]. PTC，the rice ortholog of the Arabidopsis MS1 protein[5]. EAT1 shares sequence similarity with AtbHLH089 and AtbHLH091[1,7].
-GA is a kind of important plant hormone, plays an important role in the process of plant growth and development, in some experiment show that GA signal is necessary for entry of the tapetum into PCD[7]
+GA is a kind of important plant hormone, plays an important role in the process of plant growth and development, in some experiment show that GA signal is necessary for entry of the tapetum into PCD[3,8].
 ==Labs working on this gene==