Os04g0602600 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:11:40Z

‎Structured Information

2014-06-11T15:45:00Z

2014-06-09T14:39:46Z

‎Others about the gene

2014-06-09T14:38:04Z

‎Expression

2014-06-09T14:36:30Z

‎Function

2014-06-09T14:29:14Z

‎Supporting Information

2014-06-09T14:28:13Z

‎Others about the gene

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‎Annotated Information

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‎Others about the gene

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 15:45, 11 June 2014
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−	The rice gene Os04g0602600,namely MPR25,is a pentatricopeptide repeats protein and encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria. <ref name="ref1"/>	+	The rice gene Os04g0602600,namely '''MPR25''',is a pentatricopeptide repeats protein and encodes a pentatricopeptide repeat protein and is essential for RNA editing of nad5 transcripts in mitochondria. <ref name="ref1"/>

	==Annotated Information==		==Annotated Information==

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 the coordinated interaction between mitochondria and
 chloroplasts.
 ==Supporting Information==

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 ===Expression===
 *MPR25 was preferentially expressed in leaves. FLAG-tagged MPR25 accumulated in mitochondria but not in chloroplasts. Direct sequencing revealed that the mpr25 mutant fails to edit a C–U RNA editing site at nucleotide 1580 of nad5, which encodes a subunit of complex I (NADH dehydrogenase) of the respiratory chain in mitochondria. RNA editing of this site is responsible for a change in amino acid from serine to leucine. Recombinant MPR25 directly interacted with the proximal region of the editing site of nad5 transcripts. However, the NADH dehydrogenase activity of complex I was not affected in the mutant. By contrast, genes encoding alternative NADH dehydrogenases and alternative oxidase were up-regulated. The mpr25 mutant may therefore provide new information on the coordinated interaction between mitochondria and chloroplasts.
 ===Others about the gene===

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 interacts with the proximal region of the editing site of nad5
 transcripts.
 ===Expression===

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 Additional Supporting Information may be found in the online
 version of this article:
-Figure S1. Phenotype of the mpr25 mutant grown in the dark.
+*'''Figure S1'''. Phenotype of the mpr25 mutant grown in the dark.
-Figure S2. Effect of light intensity on the chlorophyll fluorescence
+*'''Figure S2'''. Effect of light intensity on the chlorophyll fluorescence
 parameters and P700 redox state in the mpr25 mutant.
-Figure S3. Editing and expression of nad5 in various tissues.
+*'''Figure S3'''. Editing and expression of nad5 in various tissues.
-Figure S4. nad5 expression in the mpr25 mutant.
+*'''Figure S4'''. nad5 expression in the mpr25 mutant.
-Figure S5. Expression analysis of alternative NADH dehydrogenase
+*'''Figure S5'''. Expression analysis of alternative NADH dehydrogenase
 genes.
-Figure S6. Expression analysis of AOX genes.
+*'''Figure S6'''. Expression analysis of AOX genes.
-Figure S7. SDS–PAGE of purified recombinant MPR25 protein.
+*'''Figure S7'''. SDS–PAGE of purified recombinant MPR25 protein.
-Table S1. Primers used for RT-PCR and sequence analysis.
+*'''Table S1'''. Primers used for RT-PCR and sequence analysis.
 Please note: As a service to our authors and readers, this journal
 provides supporting information supplied by the authors. Such

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 the nadfs4 mutant, which lacks complex I <ref name="ref27"/>.
 The photosynthetic rate was significantly reduced in the
-mpr25 mutants (Figure 3). Similar reduction of photosynthetic
+mpr25 mutants '''(Figure 3)'''. Similar reduction of photosynthetic
 activity has also been reported in a CMSII mutant
 lacking nad7 <ref name="ref22"/>, and in an NMS1 mutant,

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 be involved in plant development.
-*MPR25 is localized to mitochondria but also related
+*MPR25 is localized to mitochondria but also related to chloroplast function
 to chloroplast function
 RNA editing of the nad5eU1580SL site in mitochondria was
 abolished in the mpr25 mutant, resulting in lack of the