Os04g0619300 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:12:45Z

‎Structured Information

Lixueliuwei: /* Function */

2014-05-27T15:02:50Z

‎Function

Lixueliuwei: /* Function */

2014-05-27T14:59:31Z

‎Function

Lixueliuwei at 14:58, 27 May 2014

2014-05-27T14:58:59Z

Lixueliuwei: /* Labs working on this gene */

2014-05-26T01:30:42Z

‎Labs working on this gene

Lixueliuwei at 16:23, 25 May 2014

2014-05-25T16:23:01Z

Lixueliuwei at 15:55, 25 May 2014

2014-05-25T15:55:41Z

Lixueliuwei at 15:54, 25 May 2014

2014-05-25T15:54:38Z

Lixueliuwei: /* Function */

2014-05-25T15:52:37Z

‎Function

Lixueliuwei: /* Function */

2014-05-25T15:51:57Z

‎Function

@@ Line 55: / Line 55: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 15:02, 27 May 2014
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−	'''GO assignment(s)''': GO:[http://amigo.geneontology.org/amigo/term/GO:0003674 0003674] GO:0005634 GO:0008150	+	'''GO assignment(s)''': GO:[http://amigo.geneontology.org/amigo/term/GO:0003674 0003674] GO:[http://amigo.geneontology.org/amigo/term/GO:0005634 0005634] GO:[http://amigo.geneontology.org/amigo/term/GO:0008150 0008150]

	===Expression===		===Expression===

← Older revision		Revision as of 14:59, 27 May 2014
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−	'''GO assignment(s)''': GO:~~0003674~~[http://amigo.geneontology.org/amigo/term/GO:0003674] GO:0005634 GO:0008150	+	'''GO assignment(s)''': GO:[http://amigo.geneontology.org/amigo/term/GO:0003674 0003674] GO:0005634 GO:0008150

	===Expression===		===Expression===

← Older revision		Revision as of 14:58, 27 May 2014
Line 14:		Line 14:


−	'''GO assignment(s)''': GO:0003674 GO:0005634 GO:0008150	+	'''GO assignment(s)''': GO:0003674[http://amigo.geneontology.org/amigo/term/GO:0003674] GO:0005634 GO:0008150

	===Expression===		===Expression===

@@ Line 34: / Line 34: @@
 ==Labs working on this gene==
-Natl Agr Res Ctr Hokkaido Reg, Hitsujigaoka 1, Sapporo, Hokkaido 0628555, Japan
+*Natl Agr Res Ctr Hokkaido Reg, Hitsujigaoka 1, Sapporo, Hokkaido 0628555, Japan
-Univ Florida, IFAS, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA
+*Univ Florida, IFAS, Ctr Citrus Res & Educ, Lake Alfred, FL 33850 USA
-N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA
+*N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA
 ==References==

@@ Line 1: / Line 1: @@
-Ctb1 is one of the quantitative trait loci (QTLs) for cold tolerance in the introgression on chromosome 4 of rice.
+''Ctb1'' is one of the quantitative trait loci (QTLs) for cold tolerance in the introgression on chromosome 4 of rice.
 ==Annotated Information==
 ===Function===
-Low temperatures during the booting stage reduce rice yields by causing cold-induced male sterility<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene Ctb1, Plant Science, 179:97–102.</ref>. Ctb1 is a QTL for cold tolerance, which is located in the introgression on chromosome 4<ref name="ref2">K. Saito, K. Miura, K. Nagano, Y. Hayano-Saito, H. Araki, A. Kato, (2001) Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length, Theor. Appl. Genet. 103: 862–868.</ref>. Researches mapped Ctb1 to a 17-kb region containing two genes that encode an F-box protein and a ser/thr protein kinase. Both genes were cloned from a cold-tolerant variety, Norin-PL8, and introduced into a cold-sensitive variety, Hokkai241, and a cold-sensitive line, BT4-74-8. The cold tolerance of T2 and T3 progenies was assessed by measuring the degree of spikelet fertility in plants treated with cool water irrigation (19◦C, 25 cm) or cool air (12◦C, 4 days).
+Low temperatures during the booting stage reduce rice yields by causing cold-induced male sterility<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene ''Ctb1'', Plant Science, 179:97–102.</ref>. ''Ctb1'' is a QTL for cold tolerance, which is located in the introgression on chromosome 4<ref name="ref2">K. Saito, K. Miura, K. Nagano, Y. Hayano-Saito, H. Araki, A. Kato, (2001) Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length, Theor. Appl. Genet. 103: 862–868.</ref>. Researches mapped ''Ctb1'' to a 17-kb region containing two genes that encode an F-box protein and a ser/thr protein kinase. Both genes were cloned from a cold-tolerant variety, Norin-PL8, and introduced into a cold-sensitive variety, Hokkai241, and a cold-sensitive line, BT4-74-8. The cold tolerance of T2 and T3 progenies was assessed by measuring the degree of spikelet fertility in plants treated with cool water irrigation (19◦C, 25 cm) or cool air (12◦C, 4 days).
@@ Line 11: / Line 11: @@
-The results indicated that the F-box protein gene confers cold tolerance. Cold tolerance is associated with greater anther length, which is one of major factors in cold tolerance at the booting stage. Ctb-1 expressed cold tolerance by producing a large anther. The transgenic plants had longer anthers than non-transformed controls. The F-box protein encoded by Ctb1 interact with a subunit of the E3 ubiquitin ligase, Skp1, suggesting that an ubiquitin–proteasome pathway is involved in cold tolerance at the booting stage<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene Ctb1, Plant Science, 179:97–102.</ref>.
+The results indicated that the F-box protein gene confers cold tolerance. Cold tolerance is associated with greater anther length, which is one of major factors in cold tolerance at the booting stage. Ctb-1 expressed cold tolerance by producing a large anther. The transgenic plants had longer anthers than non-transformed controls. The F-box protein encoded by ''Ctb1'' interact with a subunit of the E3 ubiquitin ligase, Skp1, suggesting that an ubiquitin–proteasome pathway is involved in cold tolerance at the booting stage<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene ''Ctb1'', Plant Science, 179:97–102.</ref>.
@@ Line 18: / Line 18: @@
 ===Expression===
-[[File:PCR.JPG|center|thumb|450px|''Fig. 2 RT-PCR and structural analysis of the Ctb1 candidate genes. (a) Expression of genes encoding F-box protein (FB) and a ser/thr protein kinase(PK) in mature leaves (L) and young panicles (YP) from a cold sensitive variety Kirara397 (K) and a cold-tolerant variety Norin-PL8 (N). ACTIN gene was used as a reference. (b) Diagrams of DNA fragments containing the FB and PK genes cloned from Norin-PL8 genomic DNA. Black rectangles, black triangles, and open triangles indicate putative exons, initiation codons, and stop codons, respectively  (from reference  <ref name="ref1" />).'']]
+[[File:PCR.JPG|center|thumb|450px|''Fig. 2 RT-PCR and structural analysis of the ''Ctb1'' candidate genes. (a) Expression of genes encoding F-box protein (FB) and a ser/thr protein kinase(PK) in mature leaves (L) and young panicles (YP) from a cold sensitive variety Kirara397 (K) and a cold-tolerant variety Norin-PL8 (N). ACTIN gene was used as a reference. (b) Diagrams of DNA fragments containing the FB and PK genes cloned from Norin-PL8 genomic DNA. Black rectangles, black triangles, and open triangles indicate putative exons, initiation codons, and stop codons, respectively  (from reference  <ref name="ref1" />).'']]
-Sequence analyses indicated no polymorphism between Norin-PL8 and Kirara397 in the coding regions but several polymorphisms (mostly single nucleotide polymorphisms) in the 5’ upstream regions of both F-box protein (FB) and a ser/thr protein kinase(PK). Comparing the expression of the genes in Norin-PL8 and Kirara397 by RT-PCR analysis, PK was expressed in mature leaves and young panicles. By contrast, FB was expressed preferentially in young panicles, suggesting that FB was a more likely candidate to be Ctb1. No differences were observed in expression levels between Norin-PL8 and Kirara397<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene Ctb1, Plant Science, 179:97–102.</ref>.
+Sequence analyses indicated no polymorphism between Norin-PL8 and Kirara397 in the coding regions but several polymorphisms (mostly single nucleotide polymorphisms) in the 5’ upstream regions of both F-box protein (FB) and a ser/thr protein kinase(PK). Comparing the expression of the genes in Norin-PL8 and Kirara397 by RT-PCR analysis, PK was expressed in mature leaves and young panicles. By contrast, FB was expressed preferentially in young panicles, suggesting that FB was a more likely candidate to be ''Ctb1''. No differences were observed in expression levels between Norin-PL8 and Kirara397<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene ''Ctb1'', Plant Science, 179:97–102.</ref>.
 ===Evolution===
@@ Line 44: / Line 44: @@
 <references>
-<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene Ctb1, Plant Science, 179:97–102.</ref>
+<ref name="ref1">K. Saito, Y. Hayano-Saito, M. Kuroki, Y. Sato,(2010) Map-based cloning of the rice cold tolerance gene ''Ctb1'', Plant Science, 179:97–102.</ref>
 <ref name="ref2">K. Saito, K. Miura, K. Nagano, Y. Hayano-Saito, H. Araki, A. Kato, (2001) Identification of two closely linked quantitative trait loci for cold tolerance on chromosome 4 of rice and their association with anther length, Theor. Appl. Genet. 103: 862–868.</ref>

← Older revision		Revision as of 15:55, 25 May 2014
Line 1:		Line 1:
−	Ctb1 is one of the quantitative trait loci (QTLs) for cold tolerance in the introgression on chromosome 4.	+	Ctb1 is one of the quantitative trait loci (QTLs) for cold tolerance in the introgression on chromosome 4 of rice.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 15:54, 25 May 2014
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−	~~Please input~~ one~~-sentence summary here~~.	+	Ctb1 is one of the quantitative trait loci (QTLs) for cold tolerance in the introgression on chromosome 4.

	==Annotated Information==		==Annotated Information==

← Older revision		Revision as of 15:52, 25 May 2014
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−	[[File:Anther.JPG\|left\|thumb\|600px\|''Table. 1 Cold tolerance, heading date and anther length of the near-isogenic lines(NILs) and parental varieties (from reference <ref name="ref2" />).'']][[File:Seed.JPG\|~~right~~\|thumb\|450px\|''Fig. 1. Relation between cold tolerance and anther length shown in Table 1. Circles represent the NILs: open circles represent the NILs without either Ctb-1or Ctb-2, and closed circles represent the NILs with Ctb-1and/or Ctb-2. Open and closed triangles represent Kirara397 and Norin-PL8, respectively (from reference <ref name="ref2" />).'']]	+	[[File:Anther.JPG\|left\|thumb\|600px\|''Table. 1 Cold tolerance, heading date and anther length of the near-isogenic lines(NILs) and parental varieties (from reference <ref name="ref2" />).'']][[File:Seed.JPG\|center\|thumb\|450px\|''Fig. 1. Relation between cold tolerance and anther length shown in Table 1. Circles represent the NILs: open circles represent the NILs without either Ctb-1or Ctb-2, and closed circles represent the NILs with Ctb-1and/or Ctb-2. Open and closed triangles represent Kirara397 and Norin-PL8, respectively (from reference <ref name="ref2" />).'']]

← Older revision		Revision as of 15:51, 25 May 2014
Line 7:		Line 7:


−	[[File:Anther.JPG\|left\|thumb\|~~800px~~\|''Table. 1 Cold tolerance, heading date and anther length of the near-isogenic lines(NILs) and parental varieties (from reference <ref name="ref2" />).'']][[File:Seed.JPG\|right\|thumb\|~~800px~~\|''Fig. 1. Relation between cold tolerance and anther length shown in Table 1. Circles represent the NILs: open circles represent the NILs without either Ctb-1or Ctb-2, and closed circles represent the NILs with Ctb-1and/or Ctb-2. Open and closed triangles represent Kirara397 and Norin-PL8, respectively (from reference <ref name="ref2" />).'']]	+	[[File:Anther.JPG\|left\|thumb\|600px\|''Table. 1 Cold tolerance, heading date and anther length of the near-isogenic lines(NILs) and parental varieties (from reference <ref name="ref2" />).'']][[File:Seed.JPG\|right\|thumb\|450px\|''Fig. 1. Relation between cold tolerance and anther length shown in Table 1. Circles represent the NILs: open circles represent the NILs without either Ctb-1or Ctb-2, and closed circles represent the NILs with Ctb-1and/or Ctb-2. Open and closed triangles represent Kirara397 and Norin-PL8, respectively (from reference <ref name="ref2" />).'']]