Os04g0622600 - Revision history

Xysj1980: /* Structured Information */

2017-03-27T11:53:06Z

‎Structured Information

Xysj1980: /* Structure */

2017-03-27T11:52:44Z

‎Structure

Xysj1980 at 11:52, 27 March 2017

2017-03-27T11:52:32Z

192.168.72.52: /* Structured Information */

2015-06-12T07:13:05Z

‎Structured Information

Show changes

Suli: /* Evolution */

2014-06-08T03:40:58Z

‎Evolution

Suli: /* Evolution */

2014-06-08T03:40:20Z

‎Evolution

Suli: /* Evolution */

2014-06-08T03:39:27Z

‎Evolution

Suli: /* Structure */

2014-06-08T03:37:29Z

‎Structure

Suli: /* Structure */

2014-06-08T03:36:49Z

‎Structure

Suli: /* Structure */

2014-06-08T03:36:31Z

‎Structure

← Older revision		Revision as of 11:53, 27 March 2017
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	</references>		</references>

−	~~==Structured Information==~~

	[[Category:Genes]]		[[Category:Genes]]

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 *The C-terminal half of Xa1 gene is composed of LRR, the common motif of the several classes of resistance genes.
 *XA1 protein contains 22 potential N-linked glycosylation sites. Six of these sites are at amino acid position 36 of each LRR repeat. These structural characteristics suggest that the Xa1 product interacts with other proteins in a defense reaction–signal transduction pathway<ref name="ref2" />.
 ==Labs working on this gene==

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 ==Annotated Information==
 ===Function===
-The Xa1 gene in rice confers resistance to Japanese race 1 of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial blight (BB). We isolated the Xa1 gene by a map-based cloning strategy. The deduced amino acid sequence of the Xa1 gene product contains nucleotide binding sites (NBS) and a new type of leucine-rich repeats (LRR); thus, Xa1 is a member of the NBS-LRR class of plant disease-resistance genes, but quite different from Xa21, another BB-resistance gene isolated from rice. <ref name="ref2" />
+The Xa1 gene in rice confers resistance to Japanese race 1 of Xanthomonas oryzae pv. oryzae, the causal pathogen of bacterial blight (BB). We isolated the Xa1 gene by a map-based cloning strategy. The deduced amino acid sequence of the Xa1 gene product contains nucleotide binding sites (NBS) and a new type of leucine-rich repeats (LRR); thus, Xa1 is a member of the NBS-LRR class of plant disease-resistance genes, but quite different from Xa21, another BB-resistance gene isolated from rice. <ref name="ref2" /><ref name="ref3" />
-Xa1 Is a Single-Copy Gene. Southern hybridization analysis was carried out by using the genomic DNAs from resistance line IR-BB1 and susceptible isogenic line IR24. The NBS region of Xa1 hybridized to a single band of DNA from IR-BB1 and IR24, suggesting that Xa1 is a single-copy gene and IR24 also has a sequence homologous to Xa1 at the same locus.<ref name="ref2" />
+Xa1 Is a Single-Copy Gene. Southern hybridization analysis was carried out by using the genomic DNAs from resistance line IR-BB1 and susceptible isogenic line IR24. The NBS region of Xa1 hybridized to a single band of DNA from IR-BB1 and IR24, suggesting that Xa1 is a single-copy gene and IR24 also has a sequence homologous to Xa1 at the same locus.<ref name="ref2" /><ref name="ref3" />
 ===Expression===

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 ===Evolution===
-[[File:rice3.jpg‎|right|thumb|250px|]][[File:rice2.jpg‎|right|thumb|250px|]]
+[[File:rice3.jpg‎|right|thumb|150px|]][[File:rice2.jpg‎|right|thumb|150px|]]
 Map-based cloning methods have been applied for isolation of Xa-1, one of the bacterial blight resistance genes in rice.Xa-1 was previously mapped on chromosome 4 using molecular markers. For positional cloning of Xa-1, a high-resolution genetic map was made for theXa-1 region using an F2population of 402 plants and additional molecular markers. Three restriction fragment length polymorphism (RFLP) markers, XNpb235, XNpb264 andC600 were found to be linked tightly to Xa-1, with no recombinants, and U08750 was mapped 1.5 cM from Xa-1. The screening of a yeast artificial chromosome (YAC) library using theseXa-1-linked RFLP markers resulted in the identification of ten contiguous YAC clones. Among these, one YAC clone, designated Y5212, with an insert of 340 kb, hybridized with all three tightly linked markers. This YAC was confirmed to possess the Xa-1 allele by mapping the Xa-1 gene between both end clones of this YAC (Y5212R and Y5212L).<ref name="ref1" />

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 ===Evolution===
-Map-based cloning methods have been applied for isolation of Xa-1, one of the bacterial blight resistance genes in rice.Xa-1 was previously mapped on chromosome 4 using molecular markers. For positional cloning of Xa-1, a high-resolution genetic map was made for theXa-1 region using an F2population of 402 plants and additional molecular markers. Three restriction fragment length polymorphism (RFLP) markers, XNpb235, XNpb264 andC600 were found to be linked tightly to Xa-1, with no recombinants, and U08750 was mapped 1.5 cM from Xa-1. The screening of a yeast artificial chromosome (YAC) library using theseXa-1-linked RFLP markers resulted in the identification of ten contiguous YAC clones. Among these, one YAC clone, designated Y5212, with an insert of 340 kb, hybridized with all three tightly linked markers. This YAC was confirmed to possess the Xa-1 allele by mapping the Xa-1 gene between both end clones of this YAC (Y5212R and Y5212L).<ref name="ref1" />[[File:rice3.jpg‎|right|thumb|250px|]][[File:rice2.jpg‎|right|thumb|250px|]]
+[[File:rice3.jpg‎|right|thumb|250px|]][[File:rice2.jpg‎|right|thumb|250px|]]
 Based on the deduced amino acid sequence, the Xa1 gene is a class 1 resistance gene; a cytoplasmic receptor-like protein with NBS and LRR domains. Thus, the structure is quite different from the first cloned rice resistance gene, Xa21 (16), which is a class 4 gene. The most intriguing finding is that the expression of Xa1, unlike any previously studied resistance genes, is induced by pathogen infection and wound.<ref name="ref2" />

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 ===Structure===
-*The composite nucleotide sequence of the Xa1 cDNA encoded a 5,406-bp ORF that was flanked by 59 and 39 untranslated regions of 112 and 392 bp, respectively. The nucleotide sequence of the 13.5-kb EcoRI-BamHI genomic fragment was also determined and compared with that of the cDNA sequence. The Xa1 gene was composed of four exons separated by three introns.<ref name="ref2" />
+*The composite nucleotide sequence of the Xa1 cDNA encoded a 5,406-bp ORF that was flanked by 59 and 39 untranslated regions of 112 and 392 bp, respectively. The nucleotide sequence of the 13.5-kb EcoRI-BamHI genomic fragment was also determined and compared with that of the cDNA sequence. The Xa1 gene was composed of four exons separated by three introns.<ref name="ref2" />[[File:rice1.jpg|right|thumb|250px|]]
 *The C-terminal half of Xa1 gene is composed of LRR, the common motif of the several classes of resistance genes.
 *XA1 protein contains 22 potential N-linked glycosylation sites. Six of these sites are at amino acid position 36 of each LRR repeat. These structural characteristics suggest that the Xa1 product interacts with other proteins in a defense reaction–signal transduction pathway<ref name="ref2" />.
 Identification of the Xa1 Candidate cDNA Clone by High-Resolution Mapping and Sequence Analysis.We isolated a 340-kb YAC clone, Y5212, that spans the Xa1 locus (Fig. 1A) (27). To identify expressed sequences in the region of Y5212, a cDNA library of IR-BB1 (Xa1yXa1) was constructed with vector lambda gt10 and screened with Y5212clone DNA. Thirty-three positive clones were obtained; they were classified into 21 classes based on their 39 sequences.<ref name="ref2" />

← Older revision		Revision as of 03:36, 8 June 2014
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	*XA1 protein contains 22 potential N-linked glycosylation sites. Six of these sites are at amino acid position 36 of each LRR repeat. These structural characteristics suggest that the Xa1 product interacts with other proteins in a defense reaction–signal transduction pathway<ref name="ref2" />.		*XA1 protein contains 22 potential N-linked glycosylation sites. Six of these sites are at amino acid position 36 of each LRR repeat. These structural characteristics suggest that the Xa1 product interacts with other proteins in a defense reaction–signal transduction pathway<ref name="ref2" />.
	[[File:rice1.jpg\|right\|thumb\|250px\|]]		[[File:rice1.jpg\|right\|thumb\|250px\|]]
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