Os04g0649100 - Revision history

Chenxi Sang at 03:11, 22 October 2016

2016-10-22T03:11:26Z

192.168.72.52: /* Structured Information */

2015-06-12T07:14:39Z

‎Structured Information

Daniel: /* Labs working on this gene */

2014-05-27T09:53:34Z

‎Labs working on this gene

Daniel: /* Labs working on this gene */

2014-05-27T09:49:09Z

‎Labs working on this gene

Daniel at 09:44, 27 May 2014

2014-05-27T09:44:06Z

Zhangzhang at 03:46, 25 May 2014

2014-05-25T03:46:45Z

Daniel: /* Annotated Information */

2014-05-24T23:38:50Z

‎Annotated Information

Daniel: /* Function */

2014-05-24T15:19:23Z

‎Function

Daniel: /* References */

2014-05-24T15:16:58Z

‎References

Daniel: /* Expression */

2014-05-24T15:11:58Z

‎Expression

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os04g0649100''''' was reported as '''''SHAT1''''' in 2012 <ref name="ref1" /> by researchers from China and Japan.
-Function
+==Annotated Information==
-Seed shattering is an important agricultural trait in crop domestication.SH4(for grain shattering quantitative trait locus on chromosome 4) andqSH1(for quantitative trait locus of seed shattering on chromosome 1) genes have been identified as required for reduced seed shattering during rice (Oryza sativa) domestication. However, the regulatory pathways of seed shattering in rice remain unknown. Here, we identified a seedshattering abortion1(shat1) mutant in a wild rice introgression line. The SHAT1gene, which encodes an APETALA2 transcription factor, is required for seed shattering through specifying abscission zone (AZ) development in rice. Genetic analyses revealed that the expression ofSHAT1in AZ was positively regulated by the trihelix transcription factorSH4. We also identified a frameshift mutant of SH4that completely eliminated AZs and showed nonshattering. Our results suggest a genetic model in which the persistent and concentrated expression of activeSHAT1andSH4in the AZ during early spikelet developmental stages is required for conferring AZ identification.qSH1 functioned downstream of SHAT1andSH4, through maintaining SHAT1andSH4expression in AZ, thus promoting AZ differentiation.
+* '''''OsSHAT1''''' gene  encodes an APETALA2 transcription factor.
+* '''''OsSHAT1''''' is required for seed shattering through specifyingabscission zone (AZ) development in rice.
-Expression
+===Expression===
+* Genetic analyses revealed that the expression of '''''SHAT1''''' in AZ (Abscission Zone) was positively regulated by the trihelix transcription factor SH4. You can also add sub-section(s) at will.
 ==Labs working on this gene==
-Please input related labs here.
+* National Center for Gene Research, National Center for Plant Gene Research (Shanghai) and Institute of Plant Physiology and
+Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200233, China
-Zhou Y, Lu D, Li C, et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor SHATTERING ABORTION1[J]. The Plant Cell Online, 2012, 24(3): 1034-1048.
+* Plant Genome Center, Tsukuba, Ibaraki 305-0856, Japan
+* State Key Laboratory of Systematic and Evolutionary Botany, Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
-Wollmann H, Mica E, Todesco M, et al. On reconciling the interactions between APETALA2, miR172 and AGAMOUS with the ABC model of flower development[J]. Development, 2010, 137(21): 3633-3642.
+* Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
+* Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China
-Zhu Q H, Upadhyaya N M, Gubler F, et al. Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa)[J]. BMC Plant Biology, 2009, 9(1): 149.
+==References==
 ==Structured Information==

@@ Line 32: / Line 32: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 18: / Line 18: @@
 Zhou Y, Lu D, Li C, et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor SHATTERING ABORTION1[J]. The Plant Cell Online, 2012, 24(3): 1034-1048.
 Wollmann H, Mica E, Todesco M, et al. On reconciling the interactions between APETALA2, miR172 and AGAMOUS with the ABC model of flower development[J]. Development, 2010, 137(21): 3633-3642.
 Würschum T, Groß-Hardt R, Laux T. APETALA2 regulates the stem cell niche in the Arabidopsis shoot meristem[J]. The Plant Cell Online, 2006, 18(2): 295-307.
 Chen X. A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development[J]. Science, 2004, 303(5666): 2022-2025.
 Itoh J I, Nonomura K I, Ikeda K, et al. Rice plant development: from zygote to spikelet[J]. Plant and cell physiology, 2005, 46(1): 23-47.
 Ishikawa R, Thanh P T, Nimura N, et al. Allelic interaction at seed-shattering loci in the genetic backgrounds of wild and cultivated rice species[J]. Genes & genetic systems, 2009, 85(4): 265-271.
 Zhu Q H, Upadhyaya N M, Gubler F, et al. Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa)[J]. BMC Plant Biology, 2009, 9(1): 149.

← Older revision		Revision as of 09:49, 27 May 2014
Line 18:		Line 18:

	Zhou Y, Lu D, Li C, et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor SHATTERING ABORTION1[J]. The Plant Cell Online, 2012, 24(3): 1034-1048.		Zhou Y, Lu D, Li C, et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor SHATTERING ABORTION1[J]. The Plant Cell Online, 2012, 24(3): 1034-1048.
		+	Wollmann H, Mica E, Todesco M, et al. On reconciling the interactions between APETALA2, miR172 and AGAMOUS with the ABC model of flower development[J]. Development, 2010, 137(21): 3633-3642.
		+	Würschum T, Groß-Hardt R, Laux T. APETALA2 regulates the stem cell niche in the Arabidopsis shoot meristem[J]. The Plant Cell Online, 2006, 18(2): 295-307.
		+	Chen X. A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development[J]. Science, 2004, 303(5666): 2022-2025.
		+	Itoh J I, Nonomura K I, Ikeda K, et al. Rice plant development: from zygote to spikelet[J]. Plant and cell physiology, 2005, 46(1): 23-47.
		+	Ishikawa R, Thanh P T, Nimura N, et al. Allelic interaction at seed-shattering loci in the genetic backgrounds of wild and cultivated rice species[J]. Genes & genetic systems, 2009, 85(4): 265-271.
		+	Zhu Q H, Upadhyaya N M, Gubler F, et al. Over-expression of miR172 causes loss of spikelet determinacy and floral organ abnormalities in rice (Oryza sativa)[J]. BMC Plant Biology, 2009, 9(1): 149.

	==Structured Information==		==Structured Information==

@@ Line 1: / Line 1: @@
 Please input one-sentence summary here.
-==Annotated Information==
+Function
-===Function===
 Seed shattering is an important agricultural trait in crop domestication.SH4(for grain shattering quantitative trait locus on chromosome 4) andqSH1(for quantitative trait locus of seed shattering on chromosome 1) genes have been identified as required for reduced seed shattering during rice (Oryza sativa) domestication. However, the regulatory pathways of seed shattering in rice remain unknown. Here, we identified a seedshattering abortion1(shat1) mutant in a wild rice introgression line. The SHAT1gene, which encodes an APETALA2 transcription factor, is required for seed shattering through specifying abscission zone (AZ) development in rice. Genetic analyses revealed that the expression ofSHAT1in AZ was positively regulated by the trihelix transcription factorSH4. We also identified a frameshift mutant of SH4that completely eliminated AZs and showed nonshattering. Our results suggest a genetic model in which the persistent and concentrated expression of activeSHAT1andSH4in the AZ during early spikelet developmental stages is required for conferring AZ identification.qSH1 functioned downstream of SHAT1andSH4, through maintaining SHAT1andSH4expression in AZ, thus promoting AZ differentiation.
-===Expression===
+Expression
 The Expression of SHAT1 in AZ Is Positively Regulated bySH4。To exploit the effect ofSHAT1on AZ differentiation, we examinedSHAT1expression during early floral development using in situ hybridization. The inflorescence and spikelet developmental stages used in this study were according to the criteria reported before. Our analyses ofSHAT1gene expression in the wild type showed that when lemma and palea primordia were first visible on the flanks of the floral meristem at sp6 stage,SHAT1mRNA showed hotspots of expression in palea andlemma primordia .Subsequently,SHAT1signal declined in lemma and palea but appeared in the inner floral organ primordia, such as stamens and carpels during stage sp7, when carpel primordia began to differentiate . When the ovule was first visible,SHAT1 transcripts were restricted to the AZ and anthers from early stagesp8. Afterwards, during late sp8 stage, SHAT1 expression accumulated to higher levels in the AZ than during early stage sp8. We also characterized SHAT1 expression patterns in GLA4. Its genetic background is very close to the wild type, as mentioned above, but itssh4-1allele is the cultivated rice type. Therefore, we used GLA4 as the sh4-1 mutant line in this study. TheSHAT1signal in GLA4 was very similar to that in the wild type, showing converging expression in AZ from early stage sp8 and becoming more intense during late stage sp8. By contrast, the sh4-2mutation completely disruptedSHAT1expression in AZ, with no signals observed during stages sp6-sp8, similar to that in theshat1mutant.
@@ Line 16: / Line 17: @@
 Please input related labs here.
 Zhou Y, Lu D, Li C, et al. Genetic control of seed shattering in rice by the APETALA2 transcription factor SHATTERING ABORTION1[J]. The Plant Cell Online, 2012, 24(3): 1034-1048.

@@ Line 5: / Line 5: @@
 Please input function information here.
-===Expression===
+The Expression of SHAT1 in AZ Is Positively Regulated bySH4。To exploit the effect ofSHAT1on AZ differentiation, we examinedSHAT1expression during early floral development using in situ hybridization. The inflorescence and spikelet developmental stages used in this study were according to the criteria reported before. Our analyses ofSHAT1gene expression in the wild type showed that when lemma and palea primordia were first visible on the flanks of the floral meristem at sp6 stage,SHAT1mRNA showed hotspots of expression in palea andlemma primordia .Subsequently,SHAT1signal declined in lemma and palea but appeared in the inner floral organ primordia, such as stamens and carpels during stage sp7, when carpel primordia began to differentiate . When the ovule was first visible,SHAT1 transcripts were restricted to the AZ and anthers from early stagesp8. Afterwards, during late sp8 stage, SHAT1 expression accumulated to higher levels in the AZ than during early stage sp8. We also characterized SHAT1 expression patterns in GLA4. Its genetic background is very close to the wild type, as mentioned above, but itssh4-1allele is the cultivated rice type. Therefore, we used GLA4 as the sh4-1 mutant line in this study. TheSHAT1signal in GLA4 was very similar to that in the wild type, showing converging expression in AZ from early stage sp8 and becoming more intense during late stage sp8. By contrast, the sh4-2mutation completely disruptedSHAT1expression in AZ, with no signals observed during stages sp6-sp8, similar to that in theshat1mutant.
 ===Evolution===