Os04g0659100 - Revision history

Xysj1980 at 03:47, 8 March 2017

2017-03-08T03:47:22Z

Xysj1980 at 02:47, 8 March 2017

2017-03-08T02:47:10Z

Xysj1980: /* Function */

2017-03-08T02:45:22Z

‎Function

Xysj1980 at 02:45, 8 March 2017

2017-03-08T02:45:04Z

192.168.72.52: /* Structured Information */

2015-06-12T07:15:40Z

‎Structured Information

Mtfgibh: /* GS2 impact nitrogen metabolism */

2014-05-28T11:35:56Z

‎GS2 impact nitrogen metabolism

Mtfgibh: /* References */

2014-05-28T11:32:14Z

‎References

Mtfgibh: /* GS2 impact nitrogen metabolism */

2014-05-28T11:29:53Z

‎GS2 impact nitrogen metabolism

Mtfgibh: /* Expression */

2014-05-28T11:19:50Z

‎Expression

Mtfgibh: /* References */

2014-05-28T11:13:12Z

‎References

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 ==Annotated Information==
 ===Function===
 * The rice '''''OsGS2''''' can encode chloroplastic glutamine synthetase proteins.

@@ Line 4: / Line 4: @@
 ===Function===
 * The rice '''''OsGS2''''' can encode chloroplastic glutamine synthetase proteins.
-* The rice '''''OsGS2''''' is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP. It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration.
+* The rice '''''OsGS2''''' is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP.
 ===Phenotypic analysis===
@@ Line 14: / Line 16: @@
 * The intracellular contents of NH 4 + and Na + in the stressed plants correlated well with the levels of GS2.
 * Thus, the enhancement of photorespiration conferred resistance to salt in rice plants.
 You can also add sub-section(s) at will.
 ==Labs working on this gene==

@@ Line 4: / Line 4: @@
 ===Function===
 * The rice '''''OsGS2''''' can encode chloroplastic glutamine synthetase proteins.
-* The rice '''''OsGS2''''' is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP[1]. It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration.[[File:Example.jpg]]
+* The rice '''''OsGS2''''' is a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP. It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration.
 ===Phenotypic analysis===

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os04g0659100''''' was reported as '''''OsGS2''''' in 2000 <ref name="ref1" /> by researchers from Japan.
 ==Annotated Information==
 ===Function===
-OsGS2 was also named as OsGLN2, λGS31 which encodes cytoplasmic glutamine synthelase GS2. Glutamine synthelase（GS）a key enzyme of assimilation process which catalyze glutamate and ammonia into glutamine at the existence of ATP[1]. It is the first reaction of nitrogen assimilation. GS2 could catalyze assimilation of amino produced by chloroplasts photorespiration[2].[[File:Example.jpg]]
+* The rice '''''OsGS2''''' can encode chloroplastic glutamine synthetase proteins.
-GS with cytoplasmic GS1 and chloroplast GS2 commonly exists in the form of two kinds of isozymes: GS1 by multiple genes coding, but it is the single gene encoding the GS2[4][5]. But there are exceptions, and cloned into multiple genes encoding GS2 protein in soybeans and alfalfa[6]. GS2 proteins are encoded by nuclear genes, guided by the signal peptide in chloroplast, then the exercise of functions, mainly for NH4+primary assimilation and photorespiration pathway release NH3 recycling in the chloroplast[7]. Studies have shown that excessive expression of GS2 transgenic tobacco and rice increased light breath ability and the resistance to strong light, but no differences in growth phenotype[8][9].
+===Phenotypic analysis===
 You can also add sub-section(s) at will.
@@ Line 35: / Line 31: @@
 ==Labs working on this gene==
-. Department of Crop Science, Chungbuk National University, Cheongju, 361-763, Korea
+* Research Institute, Meijo University, Nagoya, Aichi, Japan.
+* Department of Chemistry, Faculty of Science and Technology, Meijo University, Tenpaku-ku, Nagoya, Aichi, 468-8502 Japan
-. National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea
+* Plantech Research Institute, Aoba-ku, Yokohama, Kanagawa, 227-0033 Japan
+* Graduate School of Bioagricultral Science, Nagoya University, Chikusa-ku, Nagoya, Aichi, 464-8601 Japan
 ==References==
-[1] Francisco M C, Francisco R G, Angel G G, et al. Molecular physiology  of  glutamine  and  glutamate  biosynthesis  in  developing seedling of conifers[J]. Physiol Plant, 1998,103:287-294.
+<references>
+* <ref name="ref1">
-[2] Kazunari K, Tomoyuki Y, Toahihiko H, et al. Vascular bundle-specific localisation of cytosolic glutamine synthetase in rice leaves[J]. Plant Physiol, 1992,99:1481-1486.
+Hoshida H, Tanaka Y, Hibino T, Hayashi Y, Tanaka A, Takabe T, Takabe T.
+Enhanced tolerance to salt stress in transgenic rice that overexpresses
-[3] WANG Mao, SHI Yong-Chun, LIU Quan-Jun, LIU Wei-Qun. Bioinformatic Analysis of Glutamine Synthetase 2 Gene in Different Tobacco[J]. LETTERS IN BIOTECHNOLOGY, 2009,20(2):199-201.
+chloroplast glutamine synthetase. Plant Mol Biol. 2000 May;43(1):103-11. PubMed
+PMID: 10949377.
-[4] Ireland RJ, Lea PJ (1999) The enzymes of glutamine, glutamate, asparagine and aspirate metabolism. In: Singh BK (ed) Plant amino acids: biochemistry and biotechnology. Marcel Dekker, New York, pp 49–109
+</ref>
+</references>
 ==Structured Information==

@@ Line 95: / Line 95: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 32: / Line 32: @@
 Study shows that the reaction of GS and GOGAT is the main route of ammonia assimilation in higher plants under normal conditions. This is Nitrogen Metabolism flowchart by glutamine and glutamate in Plants[16][17].[[File:333.jpg]]
-Research shown that it have a 36% to 72% decrease in the concentration of NH4+ in GS2 co-suppressed plant leaves was observed[18]. This result contrasts with the results of Husted et al. [19] in GS2- silenced oilseed rape plants, which exhibited 83% reduced GS2 activity but similar tissue concentrations of NH4+.
+Research shown that it have a 36% to 72% decrease in the concentration of NH4+ in GS2 co-suppressed plant leaves was observed[18]. This result contrasts with the results of Husted et al. [19] in GS2- silenced oilseed rape plants, which exhibited 83% reduced GS2 activity but similar tissue concentrations of NH4+.[[File:444.jpg]][[File:555.jpg]]
 ==Labs working on this gene==

← Older revision		Revision as of 11:32, 28 May 2014
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	[17] 王学奎.氮钙光对小麦谷氨酰胺合成酶和氮同化的影响[D].湖北:华中农业大学,2000:9-13.		[17] 王学奎.氮钙光对小麦谷氨酰胺合成酶和氮同化的影响[D].湖北:华中农业大学,2000:9-13.
		+
		+	[18] Cai H M, Xiao J H, Zhang Q F, et al. Co-suppressed glutamine synthetase2 gene modifies nitrogen metabolism and plant growth in rice. Chinese Sci Bull,2010,
		+
		+	[19] Yoshida S, Forno D A, Cook J H, et al. Laboratory manual for physiological studies of rice. 3rd ed. Manila: International Rice Research Institute, 1976

	==Structured Information==		==Structured Information==

← Older revision		Revision as of 11:29, 28 May 2014
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	Study shows that the reaction of GS and GOGAT is the main route of ammonia assimilation in higher plants under normal conditions. This is Nitrogen Metabolism flowchart by glutamine and glutamate in Plants[16][17].[[File:333.jpg]]		Study shows that the reaction of GS and GOGAT is the main route of ammonia assimilation in higher plants under normal conditions. This is Nitrogen Metabolism flowchart by glutamine and glutamate in Plants[16][17].[[File:333.jpg]]
		+
		+	Research shown that it have a 36% to 72% decrease in the concentration of NH4+ in GS2 co-suppressed plant leaves was observed[18]. This result contrasts with the results of Husted et al. [19] in GS2- silenced oilseed rape plants, which exhibited 83% reduced GS2 activity but similar tissue concentrations of NH4+.

	==Labs working on this gene==		==Labs working on this gene==

@@ Line 12: / Line 12: @@
 Nitrogen-related genes in different rice varieties have the expression pattern of the flag leaf at different growth stages, we can see from the figure, at any time, OsGS1.1, OsGS1.2, OsGS2 are expressed in three varieties, OsGS1.1, OsGS1.2 the expression pattern of consistent, OsGS2 gene expression levels in the flag leaf of three rice varieties in the grain-filling stage is higher, and the most obvious in the Nongken and Yangdao. It is worth noting that, in maturity, OsGS2 gene expression in three varieties more consistent with the grain-filling stage, the highest expression of Yangdao, and low expression of Nipponbare, almost no expression[10].[[File:000.jpg]][[File:111.jpg]]
-Through CREP database in rice Minghui 63 full-genome DNA microarray expression differences obtained GS2 gene expression information in different tissues and organs in the growth period. The expression profile display, GS2 gene is mainly expressed in a large number of leaves and leaf sheaths during vegetative growth period, however, in roots, reproductive organs and seed germination almost no expression[11].[[File:222.jpg]]
+The expression profile data of the GS2 gene for an elite hybrid rice parent, Minghui 63 (indica), was extracted from the CREP database (http://crep.ncpgr.cn); the expression pattern of the GS2 gene in different tissues was then manually analyzed.
 Gene silencing of GS2 in oilseed rape plants reduced the amount of GS2 protein by 35%, but no difference was found in biomass production, or leaf shape, size and color compared with the wild type [13]. In another case, GS2 mutants in barley were unable to reassimilate photorespiratory NH4+ and died when grown in normal air; however, they could survive if photorespiration was suppressed by 1% (V/V) carbon dioxide (CO2) or if they were supplemented with glutamine [14,15].

← Older revision		Revision as of 11:13, 28 May 2014
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	[15] Melo P M, Lima L M, Santos I M, et al. Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids. Plant Physiol, 2003, 132: 390–399		[15] Melo P M, Lima L M, Santos I M, et al. Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids. Plant Physiol, 2003, 132: 390–399
		+
		+	[16] 汪沛洪.植物生物化学[M].北京:中国农业出版社,1993:218.
		+
		+	[17] 王学奎.氮钙光对小麦谷氨酰胺合成酶和氮同化的影响[D].湖北:华中农业大学,2000:9-13.

	==Structured Information==		==Structured Information==