Os04g0673300 - Revision history

Xysj2019 at 15:05, 6 March 2017

2017-03-06T15:05:25Z

Xysj2019 at 15:03, 6 March 2017

2017-03-06T15:03:26Z

Xysj2019 at 14:56, 6 March 2017

2017-03-06T14:56:53Z

192.168.72.52: /* Structured Information */

2015-06-12T07:20:12Z

‎Structured Information

Xunaizia: /* Evolution */

2014-05-24T03:17:16Z

‎Evolution

Xunaizia: /* Expression */

2014-05-24T03:16:50Z

‎Expression

Xunaizia: /* Expression */

2014-05-24T03:16:19Z

‎Expression

Xunaizia: /* Evolution */

2014-05-24T03:15:40Z

‎Evolution

Xunaizia: /* Evolution */

2014-05-24T03:15:14Z

‎Evolution

Xunaizia: /* Expression */

2014-05-24T03:14:15Z

‎Expression

@@ Line 7: / Line 7: @@
 ===Function===
 * '''''OsRR6''''' is a kind of CK-inducible type-A response regulator[2].The type-A response regulators are relatively small, containing a receiver domain along with short N- and C-terminal extensions.
-* '''''OsRR6''''' may play an important role in abiotic stress signaling in rice, besides acting as a component in cytokinin signaling[1].
+* '''''OsRR6''''' may play an important role in abiotic stress signaling in rice, besides acting as a component in cytokinin signaling[ <ref name="ref1" />.
 [[File:Fig1.png|300px|thumb|left|Fig.1]]
-The induction of OsRR6 by different abiotic stress stimuli provides a molecular link between stress and cytokinin signaling as well[1].
+The induction of OsRR6 by different abiotic stress stimuli provides a molecular link between stress and cytokinin signaling as well <ref name="ref1" />.
 Overexpression of OsRR6 also affected the expression of CK-responsive genes[2].
-OsRR6-ox plants displayed altered morphologies and changes in CK metabolism, probably due to changes in the gene regulatory network[2].
+OsRR6-ox plants displayed altered morphologies and changes in CK metabolism, probably due to changes in the gene regulatory network <ref name="ref2" />.
@@ Line 67: / Line 67: @@
 ==References==
+<references>
-. Mukesh Jain, Akhilesh K Tyagi: Molecular characterization and differential expression of cytokinin-responsive type-A response regulators in rice (Oryza sativa)BMC Plant Biology 2006, 6:1
+* <ref name="ref1">
+Jain M, Tyagi AK, Khurana JP. Molecular characterization and differential
-.Hirose N, Makita N, Kojima M, Kamada-Nobusada T, et al. Overexpression of a type-A response regulator alters rice morphology and cytokinin metabolism. Plant Cell Physiol. 2007,48: 523-539.
+expression of cytokinin-responsive type-A response regulators in rice (Oryza
+sativa). BMC Plant Biol. 2006 Feb 13;6:1. PubMed PMID: 16472405; PubMed Central
-.Imamura A, Hanaki N, Umeda H, Nakamura A, Suzuki T, Ueguchi C, Mizuno T: Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis. Proc Natl Acad Sci USA 1998,95:2691-2696
+PMCID: PMC1382228.
+</ref>
-.Liming Du, Fangchan Jiao, Jun Chu:The two-component signal system in rice (Oryza sativa L.): A genome-wide study of cytokinin signal perception and transduction.Genomics 2007,89: 697–707
+* <ref name="ref2">
 ==Structured Information==

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+The rice '''''Os04g0673300''''' was reported as '''''OsRR6''''' in 2006 <ref name="ref1" /> by researchers from India.
-OsRR6 is a kind of CK-inducible type-A response regulator in rice[2].
 ==Annotated Information==
 ===Function===
+* '''''OsRR6''''' is a kind of CK-inducible type-A response regulator[2].The type-A response regulators are relatively small, containing a receiver domain along with short N- and C-terminal extensions.
-OsRR6 is a kind of CK-inducible type-A response regulator[2].The type-A response regulators are relatively small, containing a receiver domain along with short N- and C-terminal extensions.
+* '''''OsRR6''''' may play an important role in abiotic stress signaling in rice, besides acting as a component in cytokinin signaling[1].
 [[File:Fig1.png|300px|thumb|left|Fig.1]]
@@ Line 18: / Line 18: @@
 '''Fig.1''': Changes in transcript levels of the OsRR6 gene in response to different stress treatments. The transcript levels of OsRR6 gene in 6-day-old light-grown seedlings treated with ABA, NaCl, mannitol and cold for 6 h, were plotted as the relative expression (fold) of the seedlings mock-treated for the same duration.
@@ Line 32: / Line 25: @@
 '''Fig.2''': OsRR6 represses shoot regeneration in rice callus. Callus was transformed with Agrobacterium carrying a binary vector pActnos/Hmz (Vec.), Act1::OsRR6 (OsRR6) or Act1::OsRR6D103E (OsRR6D103E). Calli were selected with hygromycin B and grown on regeneration agar plates for 6 weeks.
@@ Line 44: / Line 36: @@
 ===Expression===
 OsRR6 is found as repeats on the top arm of chromosome 4. This area of chromosome 4 is included in a segmental duplication with a region on the upper arm of chromosome 2  (Fig. 4)[4].
@@ Line 55: / Line 48: @@
 The transcript levels of OsRR2, 3, 4, 6, 7,and 9 were significantly higher in etiolated seedlings as compared to green seedlings (Fig. 6)[4].
 [[File:Fig5.png|300px|thumb|right|Fig.5]]
@@ Line 63: / Line 53: @@
 ===Evolution===
+* The type-A RRs are mainly composed of a receiver domain with short N- and C-terminal extensions [2], essentially similar to the E. coli response regulator (RR) CheY involved in chemotaxis, and lack a typical output domain(3). All the OsRR proteins also contain the highly conserved Lys and two Asp residues (D-D-K) in the receiver domain (Fig. 7B, C).However, OsRR6 and OsRR7 have N-terminal extensions rich in gly and asp residues (Fig. 7C). These N- and C-terminal variable regions may play a role in their localization to different cellular compartments.No homolog of OsRR6 was found within the duplicated region, suggesting the involvement of gene loss or more localized duplications[4].
@@ Line 76: / Line 63: @@
 ==Labs working on this gene==
+* Interdisciplinary Centre for Plant Genomics and Department of Plant Molecular Biology, University of Delhi South Campus, Benito
-. Mukesh Jain, Akhilesh K Tyagi and Jitendra P Khurana
+Juarez Road, New Delhi-110021, India
 ==References==

← Older revision		Revision as of 14:56, 6 March 2017
Line 69:		Line 69:

	The type-A RRs are mainly composed of a receiver domain with short N- and C-terminal extensions [2], essentially similar to the E. coli response regulator (RR) CheY involved in chemotaxis, and lack a typical output domain(3). All the OsRR proteins also contain the highly conserved Lys and two Asp residues (D-D-K) in the receiver domain (Fig. 7B, C).However, OsRR6 and OsRR7 have N-terminal extensions rich in gly and asp residues (Fig. 7C). These N- and C-terminal variable regions may play a role in their localization to different cellular compartments.No homolog of OsRR6 was found within the duplicated region, suggesting the involvement of gene loss or more localized duplications[4].		The type-A RRs are mainly composed of a receiver domain with short N- and C-terminal extensions [2], essentially similar to the E. coli response regulator (RR) CheY involved in chemotaxis, and lack a typical output domain(3). All the OsRR proteins also contain the highly conserved Lys and two Asp residues (D-D-K) in the receiver domain (Fig. 7B, C).However, OsRR6 and OsRR7 have N-terminal extensions rich in gly and asp residues (Fig. 7C). These N- and C-terminal variable regions may play a role in their localization to different cellular compartments.No homolog of OsRR6 was found within the duplicated region, suggesting the involvement of gene loss or more localized duplications[4].
−	~~[[File:Fig7.png\|300px\|thumb\|left\|Fig.7]]~~
−
−	'''Fig.7''':type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.

@@ Line 97: / Line 97: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 03:17, 24 May 2014
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	'''Fig.7''':type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.		'''Fig.7''':type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.
−
−

← Older revision		Revision as of 03:16, 24 May 2014
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	'''Fig.4''':Locations and duplications of putative cytokinin two-component regulators in the rice chromosomes (cv. Nipponbare). Ovals on the chromosomes represent centromeres.		'''Fig.4''':Locations and duplications of putative cytokinin two-component regulators in the rice chromosomes (cv. Nipponbare). Ovals on the chromosomes represent centromeres.
		+

	The OsRR genes express differentially in various organs examined, and also in response to light[1]. Most of the OsRR genes were expressed at relatively higher level in mature tissues (leaves and flowers).The majority of the type-A OsRR genes (OsRR2–9 and OsRR11) were expressed at various levels in roots, stems, leaves, and spikelets (Fig.5)([4]. OsRR6 was expressed mostly in roots and leaves.		The OsRR genes express differentially in various organs examined, and also in response to light[1]. Most of the OsRR genes were expressed at relatively higher level in mature tissues (leaves and flowers).The majority of the type-A OsRR genes (OsRR2–9 and OsRR11) were expressed at various levels in roots, stems, leaves, and spikelets (Fig.5)([4]. OsRR6 was expressed mostly in roots and leaves.

@@ Line 46: / Line 46: @@
 OsRR6 is found as repeats on the top arm of chromosome 4. This area of chromosome 4 is included in a segmental duplication with a region on the upper arm of chromosome 2  (Fig. 4)[4].
 [[File:Fig4.png|300px|thumb|right|Fig.4]]
@@ Line 55: / Line 53: @@
 The transcript levels of OsRR2, 3, 4, 6, 7,and 9 were significantly higher in etiolated seedlings as compared to green seedlings (Fig. 6)[4].
 [[File:Fig5.png|300px|thumb|right|Fig.5]]
@@ Line 64: / Line 60: @@
 '''Fig.5''':The analysis of the response of the system genes family to cytokinin by RT-PCR. RNA was isolated from roots and leaves from Nipponbare rice growing in liquid nutrient medium either with 1 μM 6-benzylaminopurine (6-BA) for 4 h or with no 6-BA. OsACTIN primers were used as a template control.

← Older revision		Revision as of 03:15, 24 May 2014
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	[[File:Fig7.png\|300px\|thumb\|left\|Fig.7]]		[[File:Fig7.png\|300px\|thumb\|left\|Fig.7]]

−	Fig.7:type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.	+	'''Fig.7''':type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.
		+
		+

← Older revision		Revision as of 03:15, 24 May 2014
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	The type-A RRs are mainly composed of a receiver domain with short N- and C-terminal extensions [2], essentially similar to the E. coli response regulator (RR) CheY involved in chemotaxis, and lack a typical output domain(3). All the OsRR proteins also contain the highly conserved Lys and two Asp residues (D-D-K) in the receiver domain (Fig. 7B, C).However, OsRR6 and OsRR7 have N-terminal extensions rich in gly and asp residues (Fig. 7C). These N- and C-terminal variable regions may play a role in their localization to different cellular compartments.No homolog of OsRR6 was found within the duplicated region, suggesting the involvement of gene loss or more localized duplications[4].		The type-A RRs are mainly composed of a receiver domain with short N- and C-terminal extensions [2], essentially similar to the E. coli response regulator (RR) CheY involved in chemotaxis, and lack a typical output domain(3). All the OsRR proteins also contain the highly conserved Lys and two Asp residues (D-D-K) in the receiver domain (Fig. 7B, C).However, OsRR6 and OsRR7 have N-terminal extensions rich in gly and asp residues (Fig. 7C). These N- and C-terminal variable regions may play a role in their localization to different cellular compartments.No homolog of OsRR6 was found within the duplicated region, suggesting the involvement of gene loss or more localized duplications[4].
		+	[[File:Fig7.png\|300px\|thumb\|left\|Fig.7]]

−		+	Fig.7:type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.
−
−	~~[[File:Fig7.png\|200px\|thumb\|left\|~~Fig.7:type-A response regulators in rice. (A) Exon-intron organization of OsRR genes. Exons and introns are represented by black boxes and lines, respectively. (B) Schematic representation of OsRR proteins (aligned with respect to the second conserved Asp (D) residue). The conserved receiver domain is represented as gray box with D-D-K residues. The black vertical bars represent intron position. The numbers 0, 1 and 2 above the vertical bars represent the phase 0, 1 and 2 introns, respectively. (C) Multiple alignments of the OsRR proteins obtained with ClustalX. Fully and partially conserved (present in more than 50% of aligned sequences) residues are highlighted in black and gray boxes, respectively. Gaps (marked with dashes) have been introduced to maximize the alignments. The conserved receiver domain has been underlined. Conserved Asp and Lys residues are marked with asterisks.]]
−

← Older revision		Revision as of 03:14, 24 May 2014
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	The OsRR genes express differentially in various organs examined, and also in response to light[1]. Most of the OsRR genes were expressed at relatively higher level in mature tissues (leaves and flowers).The majority of the type-A OsRR genes (OsRR2–9 and OsRR11) were expressed at various levels in roots, stems, leaves, and spikelets (Fig.5)([4]. OsRR6 was expressed mostly in roots and leaves.		The OsRR genes express differentially in various organs examined, and also in response to light[1]. Most of the OsRR genes were expressed at relatively higher level in mature tissues (leaves and flowers).The majority of the type-A OsRR genes (OsRR2–9 and OsRR11) were expressed at various levels in roots, stems, leaves, and spikelets (Fig.5)([4]. OsRR6 was expressed mostly in roots and leaves.

		+	The transcript levels of OsRR2, 3, 4, 6, 7,and 9 were significantly higher in etiolated seedlings as compared to green seedlings (Fig. 6)[4].

−
−
−	~~The transcript levels of OsRR2, 3, 4, 6, 7,and 9 were significantly higher in etiolated seedlings as compared to green seedlings (Fig. 6)[4].~~

	[[File:Fig5.png\|300px\|thumb\|right\|Fig.5]]		[[File:Fig5.png\|300px\|thumb\|right\|Fig.5]]