Os04g0690800 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:21:52Z

‎Structured Information

Lixinmei21: /* sequence */

2014-06-08T08:58:21Z

‎sequence

Lixinmei21: /* References */

2014-06-08T08:58:03Z

‎References

Lixinmei21: /* Function */

2014-06-08T08:57:39Z

‎Function

Lixinmei21: /* References */

2014-06-08T08:56:33Z

‎References

Lixinmei21: /* sequence */

2014-06-08T08:40:19Z

‎sequence

Lixinmei21: /* Expression */

2014-06-08T08:28:24Z

‎Expression

Lixinmei21: /* Expression */

2014-06-08T08:05:00Z

‎Expression

Lixinmei21: /* Function */

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‎Function

Lixinmei21: /* References */

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‎References

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 Two psbS genes were found in the rice genome by BLASTN in the NCBI database based
 on the sequence of psbSofArabidopsis thaliana. PsbS2 is located on chromosome.The number of registered expressed sequence tags (ESTs) in leaves (total ESTs, 171,890) of psbS1 was 50, while that of psbS2 was six based on the NCBI UniGene database (http://www.ncbi.nlm.nih.gov/unigene),suggesting that psbS1 is expressed more than psbS2.Phylogenetic analysis showed that PsbS1 had high sequence homology with proteins from other Gramineae plants, while PsbS2 rather resembled proteins from dicot species
-,although the bootstrap values are too low to draw any unambiguous conclusions regarding the rather unexpected position of psbS2
+,although the bootstrap values are too low to draw any unambiguous conclusions regarding the rather unexpected position of psbS2.
 ==Labs working on this gene==

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 ==References==
-<ref name="ref1">Satoshi Ishida[1,4], Ken-ichi Morita[1,4], Masahiro Kishine[1], Atsushi Takabayashi[1], Reiko Murakami[1],Satomi Takeda[2], Ko Shimamoto[3], FumihikoSat[1]  and Tsuyoshi Endo[1].Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice[J]. Cell Physiol (2011), 52 (10): 1822-1831.</ref>
+Satoshi Ishida[1,4], Ken-ichi Morita[1,4], Masahiro Kishine[1], Atsushi Takabayashi[1], Reiko Murakami[1],Satomi Takeda[2], Ko Shimamoto[3], FumihikoSat[1]  and Tsuyoshi Endo[1].Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice[J]. Cell Physiol (2011), 52 (10): 1822-1831.
 ==Structured Information==

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 ==Function==
-PsbS is associated with the reversible de-epoxidation of violaxanthin to zeaxanthin, the so-called xanthophyll cycle[1].
+PsbS is associated with the reversible de-epoxidation of violaxanthin to zeaxanthin, the so-called xanthophyll cycle<ref name="ref1">Satoshi Ishida[1,4], Ken-ichi Morita[1,4], Masahiro Kishine[1], Atsushi Takabayashi[1], Reiko Murakami[1],Satomi Takeda[2], Ko Shimamoto[3], FumihikoSat[1]  and Tsuyoshi Endo[1].Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice[J]. Cell Physiol (2011), 52 (10): 1822-1831.</ref>.
-PsbS regulates CO2 assimilation rate when light fluctuates. Its content has significant influence on the chloroplasts protective energy dissipation (chloroplastic protective energy dissipation, qE). Chl fluorescence as energy quenching(qE) depends on the presence of the PsbS subunit[1].
+PsbS regulates CO2 assimilation rate when light fluctuates. Its content has significant influence on the chloroplasts protective energy dissipation (chloroplastic protective energy dissipation, qE). Chl fluorescence as energy quenching(qE) depends on the presence of the PsbS subunit<ref name="ref1">Satoshi Ishida[1,4], Ken-ichi Morita[1,4], Masahiro Kishine[1], Atsushi Takabayashi[1], Reiko Murakami[1],Satomi Takeda[2], Ko Shimamoto[3], FumihikoSat[1]  and Tsuyoshi Endo[1].Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice[J]. Cell Physiol (2011), 52 (10): 1822-1831.</ref>.
 ==Expression==

@@ Line 20: / Line 20: @@
 ==References==
-Satoshi Ishida[1,4], Ken-ichi Morita[1,4], Masahiro Kishine[1], Atsushi Takabayashi[1], Reiko Murakami[1],Satomi Takeda[2], Ko Shimamoto[3], FumihikoSat[1]  and Tsuyoshi Endo[1].Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice[J]. Cell Physiol (2011), 52 (10): 1822-1831.
+<ref name="ref1">Satoshi Ishida[1,4], Ken-ichi Morita[1,4], Masahiro Kishine[1], Atsushi Takabayashi[1], Reiko Murakami[1],Satomi Takeda[2], Ko Shimamoto[3], FumihikoSat[1]  and Tsuyoshi Endo[1].Allocation of Absorbed Light Energy in PSII to Thermal Dissipations in the Presence or Absence of PsbS Subunits of Rice[J]. Cell Physiol (2011), 52 (10): 1822-1831.</ref>
 ==Structured Information==

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 To confirm the RNAi effect, the expression ofpsbS genes in T2 transgenic rice was analyzed (Fig. 1A). Quantitative reverse transcription–PCR (RT–PCR) analysis revealed that the amounts of bothpsbS1 andpsbS2 transcripts were significantly reduced in the �1&2-1 and �1&2-2 lines. On the other hand,psbS gene expression in the�2 line was not distinguishable from that in the wild type, suggesting that gene silencing of psbS2 in the�2 line did not work. Immunoblot analysis using anti-PsbS antibody showed that the accumulation of PsbS protein was not detected in the �1&2-1 and �1&2-2 lines(Fig. 1B). Two isoproteins derived from each of the homolog genes could not be distinguished by SDS–PAGE analysis. These results indicate that the expression of psbS genes in the� 1&2-1 and� 1&2-2 lines was successfully silenced by RNAi[1].[[File:Expression of psbS genes in transgenic plants.png]]
-===Evolution===
+===sequence===
-Please input evolution information here.
+Two psbS genes were found in the rice genome by BLASTN in the NCBI database based
+on the sequence of psbSofArabidopsis thaliana. PsbS2 is located on chromosome.We compared their deduced amino acid sequences (Fig. S1A) using the CLUSTAL W algorithm (Thompson et al. 1994). The number of registered expressed sequence tags (ESTs) in leaves (total ESTs, 171,890) of psbS1 was 50, while that of psbS2 was six based on the NCBI UniGene database (http://www.ncbi.nlm.nih.gov/unigene),suggesting that psbS1 is expressed more than psbS2.Phylogenetic analysis showed that PsbS1 had high sequence homology with proteins from other Gramineae plants, while PsbS2 rather resembled proteins from dicot species
-You can also add sub-section(s) at will.
+(Fig. S1B), although the bootstrap values are too low to draw any unambiguous conclusions regarding the rather unexpected position of psbS2
 ==Labs working on this gene==