Os05g0186300 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:31:14Z

‎Structured Information

Huanghs at 10:34, 2 June 2014

2014-06-02T10:34:26Z

Huanghs at 10:32, 2 June 2014

2014-06-02T10:32:37Z

Huanghs: /* Labs working on this gene */

2014-06-02T10:31:02Z

‎Labs working on this gene

Huanghs: /* Labs working on this gene */

2014-06-02T10:20:12Z

‎Labs working on this gene

Huanghs: /* Function */

2014-06-02T09:26:38Z

‎Function

Huanghs: /* Evolution */

2014-06-02T09:24:57Z

‎Evolution

Huanghs: /* Expression */

2014-06-02T09:24:36Z

‎Expression

Huanghs: /* Function */

2014-06-02T09:23:54Z

‎Function

Huanghs: /* Evolution */

2014-06-02T09:23:08Z

‎Evolution

@@ Line 57: / Line 57: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 1: / Line 1: @@
 Gene Os05g0186300, namely '''OsNADP-ME3''', is one of the cytoplasmic NADP-MEs of Rice NADP-MEs, and could improve plant resistance to abiotic stresses to a certain extent.<ref name="ref1" />
 [mailto:huanghuasheng@xtbg.ac.cn email me]
 ==Annotated Information==

@@ Line 1: / Line 1: @@
-Os05g0186300, namely '''OsNADP-ME3''', is one of the cytoplasmic NADP-MEs of Rice NADP-MEs, and could improve plant resistance to abiotic stresses to a certain extent.<ref name="ref1" />
+Gene Os05g0186300, namely '''OsNADP-ME3''', is one of the cytoplasmic NADP-MEs of Rice NADP-MEs, and could improve plant resistance to abiotic stresses to a certain extent.<ref name="ref1" />
 [mailto:huanghuasheng@xtbg.ac.cn email me]
 ==Annotated Information==

@@ Line 37: / Line 37: @@
 ==Labs working on this gene==
-●Key Laboratory of Ministry of Education for Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University.
+◑Key Laboratory of Ministry of Education for Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University.
-●Asian Natural Environmental Science Center (ANESC), University of Tokyo.
+◑Asian Natural Environmental Science Center (ANESC), University of Tokyo.
-●State Key Laboratory of Rice Biology,China National Rice Research Institute and Zhejiang University.
+◑State Key Laboratory of Rice Biology,China National Rice Research Institute and Zhejiang University.
 [[File:OsNADP-ME3_evo.jpg‎|frame|25px|'''Figure 4''' Homology analysis of the amino acid sequence in rice NADP-ME gene family]]

@@ Line 37: / Line 37: @@
 ==Labs working on this gene==
-Key Laboratory of Ministry of Education for Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University.
+●Key Laboratory of Ministry of Education for Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University.
-Asian Natural Environmental Science Center (ANESC), University of Tokyo.
+●Asian Natural Environmental Science Center (ANESC), University of Tokyo.
-State Key Laboratory of Rice Biology,China National Rice Research Institute and Zhejiang University.
+●State Key Laboratory of Rice Biology,China National Rice Research Institute and Zhejiang University.
 [[File:OsNADP-ME3_evo.jpg‎|frame|25px|'''Figure 4''' Homology analysis of the amino acid sequence in rice NADP-ME gene family]]

@@ Line 11: / Line 11: @@
 lines of pBI121-NADP-ME3]]
-Choose these three strains to be materials, contrasted with WT.Through the changes of root length under stress situation of different concentrations of NaCl,Manntiol and H2O2 with these three strains, it shows that, there is no difference between WT and GM(Genetically Modified)('''Figure 2A;Figure 2E;Figure 2I'''),but after 20d with NaCl, the growing states of GM was better than WT, and in medium of 150mmol/L NaCl, lateral roots of GM was more than that of WT, and the leaf was bigger than WT('''Figure 4C''').The measuring result of root length also showed that the growing states of GM was better than WT, and value exhibited that GM is 1cm longer than WT('''Figure 4D''').All indicated that OsNADP-ME3 can increase halotolerancy of plant to some extent.<ref name="ref1" />
+Choose these three strains to be materials, contrasted with WT.Through the changes of root length under stress situation of different concentrations of NaCl,Manntiol and H2O2 with these three strains, it shows that, there is no difference between WT and GM(Genetically Modified)('''Figure 2A''';'''Figure 2E''';'''Figure 2I'''),but after 20d with NaCl, the growing states of GM was better than WT, and in medium of 150mmol/L NaCl, lateral roots of GM was more than that of WT, and the leaf was bigger than WT('''Figure 4C''').The measuring result of root length also showed that the growing states of GM was better than WT, and value exhibited that GM is 1cm longer than WT('''Figure 4D''').All indicated that OsNADP-ME3 can increase halotolerancy of plant to some extent.<ref name="ref1" />
 [[File:OS8.jpg‎|frame|'''Figure 2''' Figure 4 Analysis of the resistance of the overexpressed transgenic plants with NADP-ME3 under salt stress
 Note: A,E,I: Controls; B~C: NaCl stress condition; D: The root lengths of transgenic plants under NaCl stress condition; F~G: Manntiol

@@ Line 33: / Line 33: @@
 ===Evolution===
-'''Rice''' NADP-MEs are a multigene family of three cytoplasmic NADP-MEs and one plastidic NADP-ME. NADP-ME3 is of one of the cytoplasmic members . The cDNA sequence of the cloned NADP-ME3 is 2 240 bp, and the 5' untranslated region (UTR) is 151 bp while the 3' UTR is 376 bp. The open reading frame is 1 712 bp, encoding 570 amino
+Rice NADP-MEs are a multigene family of three cytoplasmic NADP-MEs and one plastidic NADP-ME. NADP-ME3 is of one of the cytoplasmic members . The cDNA sequence of the cloned NADP-ME3 is 2 240 bp, and the 5' untranslated region (UTR) is 151 bp while the 3' UTR is 376 bp. The open reading frame is 1 712 bp, encoding 570 amino
 acids.Homology analysis of the amino acid sequence in rice NADP-ME gene family('''Figure 4''').<ref name="ref1" />

@@ Line 24: / Line 24: @@
 ===Expression===
-'''The''' expressions of NADP-ME3 mRNA under a variety of abiotic stresses were detected with Northern blot by extracting RNA from leaves and roots of rice with different adversity handling('''Figure 3''').<ref name="ref1" />
+The expressions of NADP-ME3 mRNA under a variety of abiotic stresses were detected with Northern blot by extracting RNA from leaves and roots of rice with different adversity handling('''Figure 3''').<ref name="ref1" />
-'''The''' results indicated that,under the condition of salt stress(80 mmol/L NaCl), the expression level of NADP-ME3 in the initial period of leaf stress is relatively high. With time increasing, it decreased gradually, and the decline trend was evident.Meantime, under the same condition, although the expression level is low in root at the initial stage, it gradually increased with the time prolonging, which demonstrated that there exists response relationship between NADP-ME3 and salt stress; under the condition of alkaline stress(30 mmol/L NaHCO3),in leaf, the expression level of NADP-ME3 in the initial 6 hours is relatively high, and then showed the trend of decreasing,while in root, it was increasing (6h),and then gradually decreased.After 24h,it increased again with irregular change,but at any time, it was higher than the contrast,which illustrated that OsNADP-ME3 expressed induced by NaHCO3; under the condition of drought stress(10% PEG),in leaf, the expression level of NADP-ME3 in the initial stage is highest.With stress time prolonging,the transciriptional level of mRNA decreased gradually,while in root, it is relatively high in the initial stage, and up to highest at 12h,then declined mildly.<ref name="ref1" />
+The results indicated that,under the condition of salt stress(80 mmol/L NaCl), the expression level of NADP-ME3 in the initial period of leaf stress is relatively high. With time increasing, it decreased gradually, and the decline trend was evident.Meantime, under the same condition, although the expression level is low in root at the initial stage, it gradually increased with the time prolonging, which demonstrated that there exists response relationship between NADP-ME3 and salt stress; under the condition of alkaline stress(30 mmol/L NaHCO3),in leaf, the expression level of NADP-ME3 in the initial 6 hours is relatively high, and then showed the trend of decreasing,while in root, it was increasing (6h),and then gradually decreased.After 24h,it increased again with irregular change,but at any time, it was higher than the contrast,which illustrated that OsNADP-ME3 expressed induced by NaHCO3; under the condition of drought stress(10% PEG),in leaf, the expression level of NADP-ME3 in the initial stage is highest.With stress time prolonging,the transciriptional level of mRNA decreased gradually,while in root, it is relatively high in the initial stage, and up to highest at 12h,then declined mildly.<ref name="ref1" />
-'''In''' conclusion, at the exposure to NaCl, NaHCO3 and PEG, the expression level of NADP-ME3 changed with the different lengths of exposure duration. Therefore, the NADP-ME3 may be involved in response to abiotic stresses.<ref name="ref1" />
+In conclusion, at the exposure to NaCl, NaHCO3 and PEG, the expression level of NADP-ME3 changed with the different lengths of exposure duration. Therefore, the NADP-ME3 may be involved in response to abiotic stresses.<ref name="ref1" />
 [[File:OsNADP-ME3.jpeg‎|frame|'''Figure 3''' Changes of the expression level of NADP-ME3 gene from rice under the abiotic stresses]]

@@ Line 3: / Line 3: @@
 ==Annotated Information==
 ===Function===
-'''OsNADP-ME3''' is a malic enzyme, and malic enzymes widely exist in plant.Malic enzyme is a key enzyme in the KERBS CYCLE. This is very important to the metabolism of organisms.In plants, it takes part in photosynthesis,respiration,production of lipids and other important metabolic pathways,which is very important to keep the osmotic potential of plant cells, stabilize the PH of cytoplasm and keep the ions absorption of plant roots balanced.It is one fo the most important enzymes in life activities of plants.<ref name="ref2" /><ref name="ref3" />
+OsNADP-ME3 is a malic enzyme, and malic enzymes widely exist in plant.Malic enzyme is a key enzyme in the KERBS CYCLE. This is very important to the metabolism of organisms.In plants, it takes part in photosynthesis,respiration,production of lipids and other important metabolic pathways,which is very important to keep the osmotic potential of plant cells, stabilize the PH of cytoplasm and keep the ions absorption of plant roots balanced.It is one fo the most important enzymes in life activities of plants.<ref name="ref2" /><ref name="ref3" />
-'''The''' overexpression of OsNADP-ME3 in Arabidopsis thaliana obviously increases its stress resistance.<ref name="ref4" />OsNADP-ME3 was carried over into Arabidopsis plants. Through Northern detection,we find that No.5,No.9 and No.10 of strain are higher('''Figure 1''').<ref name="ref1" />
+The overexpression of OsNADP-ME3 in Arabidopsis thaliana obviously increases its stress resistance.<ref name="ref4" />OsNADP-ME3 was carried over into Arabidopsis plants. Through Northern detection,we find that No.5,No.9 and No.10 of strain are higher('''Figure 1''').<ref name="ref1" />
 [[File:OS7.jpg‎|frame|'''Figure 1''' Expression of the NADP-ME3 in wild-type (WT) and
 transgenic lines
@@ Line 11: / Line 11: @@
 lines of pBI121-NADP-ME3]]
-'''Choose''' these three strains to be materials, contrasted with WT.Through the changes of root length under stress situation of different concentrations of NaCl,Manntiol and H2O2 with these three strains, it shows that, there is no difference between WT and GM(Genetically Modified)('''Figure 2A;Figure 2E;Figure 2I'''),but after 20d with NaCl, the growing states of GM was better than WT, and in medium of 150mmol/L NaCl, lateral roots of GM was more than that of WT, and the leaf was bigger than WT('''Figure 4C''').The measuring result of root length also showed that the growing states of GM was better than WT, and value exhibited that GM is 1cm longer than WT('''Figure 4D''').All indicated that OsNADP-ME3 can increase halotolerancy of plant to some extent.<ref name="ref1" />
+Choose these three strains to be materials, contrasted with WT.Through the changes of root length under stress situation of different concentrations of NaCl,Manntiol and H2O2 with these three strains, it shows that, there is no difference between WT and GM(Genetically Modified)('''Figure 2A;Figure 2E;Figure 2I'''),but after 20d with NaCl, the growing states of GM was better than WT, and in medium of 150mmol/L NaCl, lateral roots of GM was more than that of WT, and the leaf was bigger than WT('''Figure 4C''').The measuring result of root length also showed that the growing states of GM was better than WT, and value exhibited that GM is 1cm longer than WT('''Figure 4D''').All indicated that OsNADP-ME3 can increase halotolerancy of plant to some extent.<ref name="ref1" />
 [[File:OS8.jpg‎|frame|'''Figure 2''' Figure 4 Analysis of the resistance of the overexpressed transgenic plants with NADP-ME3 under salt stress
 Note: A,E,I: Controls; B~C: NaCl stress condition; D: The root lengths of transgenic plants under NaCl stress condition; F~G: Manntiol
@@ Line 17: / Line 17: @@
 lengths of transgenic plants under H2O2 stress condition]]
-'''Under''' the condition of manntiol, the growing states of GM strains is better than that of WT, but it is not obvious under 250 mmol/L Manntiol('''Figure 4F''').When 275 mmol/L, it was a little better than WT('''Figure 4G'''). The measuring result of root length also showed that the growing states between GM and WT was not obvious, which showed that OsNADP-ME3 may be not relative with drought tolerance.<ref name="ref1" />
+Under the condition of manntiol, the growing states of GM strains is better than that of WT, but it is not obvious under 250 mmol/L Manntiol('''Figure 4F''').When 275 mmol/L, it was a little better than WT('''Figure 4G'''). The measuring result of root length also showed that the growing states between GM and WT was not obvious, which showed that OsNADP-ME3 may be not relative with drought tolerance.<ref name="ref1" />
-'''Under''' the condition of H2O2,although the root length of GM strains and WT was not obvious when it was 1 mmol/L, leaf of former was bigger than the latter,and growing states the same. As H2O2 concentration was added up to 1.5 mmol/L, all were obvious. Not only the root of GM strain was longer than WT,but also the leaf was much bigger. This indicated that OsNADP-ME3 can increase the antioxidant capacity.<ref name="ref1" />
+Under the condition of H2O2,although the root length of GM strains and WT was not obvious when it was 1 mmol/L, leaf of former was bigger than the latter,and growing states the same. As H2O2 concentration was added up to 1.5 mmol/L, all were obvious. Not only the root of GM strain was longer than WT,but also the leaf was much bigger. This indicated that OsNADP-ME3 can increase the antioxidant capacity.<ref name="ref1" />
-'''In''' summary, the GM strain grows better than WT under the condition of  salt-stress and oxidative intimidation,which indicates that OsNADP-ME3 improve plant resistance to abiotic stresses to a certain extent.<ref name="ref1" /> Correlative study shows that salt-stress can induce a great deal of active radical,and causes ROS oxidative intimidation.<ref name="ref5" />This moment, strain could regulate the balance of oxidation by antioxidant and antioxidase,while NADP-ME can produce  NADPH in malic acid metabolism. NADP can offer reducing power to the metabolism of ROS, which could resist the harm to plant caused by salt-stress.<ref name="ref6" />
+In summary, the GM strain grows better than WT under the condition of  salt-stress and oxidative intimidation,which indicates that OsNADP-ME3 improve plant resistance to abiotic stresses to a certain extent.<ref name="ref1" /> Correlative study shows that salt-stress can induce a great deal of active radical,and causes ROS oxidative intimidation.<ref name="ref5" />This moment, strain could regulate the balance of oxidation by antioxidant and antioxidase,while NADP-ME can produce  NADPH in malic acid metabolism. NADP can offer reducing power to the metabolism of ROS, which could resist the harm to plant caused by salt-stress.<ref name="ref6" />
 ===Expression===