Os05g0586200 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:46:15Z

‎Structured Information

Shixing: Undo revision 169579 by Shixing (talk)

2014-05-19T01:27:07Z

Undo revision 169579 by Shixing (talk)

Shixing: /* Annotated Information */

2014-05-19T01:26:03Z

‎Annotated Information

Shixing: Undo revision 169573 by Shixing (talk)

2014-05-18T19:22:06Z

Undo revision 169573 by Shixing (talk)

Shixing at 19:06, 18 May 2014

2014-05-18T19:06:37Z

Shixing: Undo revision 169571 by Shixing (talk)

2014-05-18T19:05:06Z

Undo revision 169571 by Shixing (talk)

Shixing: /* Annotated Information */

2014-05-18T18:35:37Z

‎Annotated Information

Shixing: /* Annotated Information */

2014-05-18T18:34:24Z

‎Annotated Information

Shixing: /* References */

2014-05-18T18:32:18Z

‎References

Shixing: /* Labs working on this gene */

2014-05-18T18:13:32Z

‎Labs working on this gene

@@ Line 63: / Line 63: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

@@ Line 1: / Line 1: @@
-''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.
+''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase, belongs to class I ''GH3'' protein family in rice,and it is required for the response to wounding.
 ==Annotated Information==
 ===Function===

@@ Line 1: / Line 1: @@
 ''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.
 ==Annotated Information==
 ===Function===

@@ Line 1: / Line 1: @@
-''''OsJAR1'''',one kind of JA-Ile synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.
+''OsJAR1'',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.
 ==Annotated Information==
 ===Function===
 [[File:Expression.figure1.png|thumb|right|'''Figure1:'''''Gene expression and hormone content in wild type and OsJAR1 TOS17 insertion lines after simulated herbivory (WOS)(frome reference<ref name=ref1/>).'']]
 [[File:Expression.figure2.png|thumb|right|'''Figure2:'''''The expression levels of OsJAR1 and OsJAR2 mRNA and the accumulation of JA and JA-amino acid conjugates in response to wounding(from reference<ref name=ref2/>).'']]
-OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.<ref name=ref4/>It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that OsJAR1 participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.OsJar1 modulates light and JA signalling in the photomorphogenesis of rice.<ref name=ref3/> Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. OsJAR1 is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.<ref name=ref5/>Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS<ref name=ref1/>.
+''OsJAR1'' contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine(JA-Ile) involved in defense responses in rice by the use of an osjar1 Tos17 mutant.<ref name=ref4/>It is involved in phytochrome and jasmonate signalling. In far-red and blue lights, osjar1 coleoptiles were longer if compared with the wild type (WT), indicating that ''OsJAR1'' participates in the suppression of coleoptile elongation in these light conditions, while the mutant did not show a clear phenotype in red light.''OsJar1'' modulates light and JA signalling in the photomorphogenesis of rice.<ref name=ref3/> Light can induce jasmonate-isoleucine conjugation via OsJAR1-dependent pathways in rice. ''OsJAR1'' is the most important JA-Ile conjugating enzyme in the wounding response during the vegetative stage.<ref name=ref5/>Plants produce jasmonic acid (JA) and its amino acid conjugate, jasmonoyl‐L‐isoleucine (JA‐Ile) as major defense signals in response to wounding and herbivory. OsJAR1‐deficient plants have strongly reduced JA‐Ile levels after WOS<ref name=ref1/>.
 ===Expression===
-(1)OsJar1 transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)<ref name=ref3/>.The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile.<ref name=ref2/>
+(1)''OsJar1'' transcripts were up-regulated transiently in response to treatment with exogenous methyl-jasmonic acid (MeJA)<ref name=ref3/>.The expression levels of ''OsJAR1'' and ''OsJAR2'' were induced in response to wounding with the concomitant accumulation of JA-Ile.<ref name=ref2/>
 (2)Real-time PCR was conducted according to the QuantiTect SYBR Green PCR handbook (Qiagen). PCR was perfomed in ABI PRISM 7000 cycler (PE Applied Biosystems, Warrington, UK) using QuantiTect SYBR green-mastermix with HotStar Taq DNA polymerase following the recommended conditions [15 min 95 °C, 40× (15 s, 94 °C; 30 s, 60 °C; 30 s, 72 °C)]. JAR1F2 and JAR1R2 were used as primers to amplify OsJar1 cDNA, Rubg237F and Rubg304R for ubiquitin, which was used as the endogenous control gene.
@@ Line 30: / Line 30: @@
 ===Evolution===
-OsJar1 belongs to the GH3 family in rice. Another member of this family in Arabidopsis, DWARF IN LIGHT 2 (DFL2), showed 47% identities. Dfl2 was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003). OsJAR1 contained three domains described to be important for the enzymatic function of GH3 proteins (Chang, Xiang & Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).<ref name=ref3/>
+''OsJar1'' belongs to the ''GH3'' family in rice. Another member of this family in Arabidopsis, ''DWARF IN LIGHT 2'' (DFL2), showed 47% identities. ''Dfl2'' was shown to be a light-dependent gene, but its gene product did not show enzymatic activity on plant hormones, presumably (Takase et al. 2003).'' OsJAR1'' contained three domains described to be important for the enzymatic function of ''GH3'' proteins (Chang, Xiang & Dunaway-Mariano 1997; Staswick et al. 2002; Terol et al. 2006).<ref name=ref3/>
 You can also add sub-section(s) at will.

@@ Line 1: / Line 1: @@
-''''OsJAR1'''',one kind of Jasmonoyl-isoleucine (JA-Ile) synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.
+''''OsJAR1'''',one kind of JA-Ile synthase,belongs to class I GH3 progein family in rice,and it is required for the response to wounding.
 ==Annotated Information==
 ===Function===

@@ Line 46: / Line 46: @@
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==

@@ Line 37: / Line 37: @@
 ==Labs working on this gene==
-Please input related labs here.
+*Biotechnology Research Center, The University of Tokyo, Tokyo 113-8657, Japan.
 ==References==