Os05g0595300 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:46:48Z

‎Structured Information

Xiaonan: /* Expression */

2014-05-27T08:12:32Z

‎Expression

Xiaonan: /* Expression */

2014-05-27T08:06:08Z

‎Expression

Xiaonan: /* Function */

2014-05-27T07:50:05Z

‎Function

Xiaonan: /* Expression */

2014-05-27T07:49:26Z

‎Expression

Xiaonan: /* References */

2014-05-27T07:44:18Z

‎References

Xiaonan: /* References */

2014-05-27T07:42:58Z

‎References

Xiaonan: /* Function */

2014-05-27T07:40:35Z

‎Function

Xiaonan: /* Function */

2014-05-27T07:30:09Z

‎Function

Xiaonan: /* Function */

2014-05-27T07:29:39Z

‎Function

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 08:12, 27 May 2014
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	===Expression===		===Expression===
		+	[[File:Knock-Down of NRRa and NRRb.png\|right\|thumb\|150px\|"Knock-Down of NRRa and NRRb Resulted in Enhanced Root Growth under Various Nutritional Conditions.t.(from reference <ref name="ref1" />)."]]

	NRR expresses constitutively in rice tissues of root, stem, leaf,and panicle, and its expression level in seedling roots was enhanced under N, P, or NPK combined nutrient starvation.		NRR expresses constitutively in rice tissues of root, stem, leaf,and panicle, and its expression level in seedling roots was enhanced under N, P, or NPK combined nutrient starvation.

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 NRR expresses constitutively in rice tissues of root, stem, leaf,and panicle, and its expression level in seedling roots was enhanced under N, P, or NPK combined nutrient starvation.
-Expression of NRR in rice seedling roots was significantly influenced by deficiency of macronutrients. Knock-down of expression of NRRa or NRRb by RNA interference resulted in enhanced rice root growth. By contrast, overexpression of NRRa in rice exhibited significantly retarded root growth. These results revealed that both NRRa and NRRb played negative regulatory roles in rice root growth.
+Overexpressing or RNAi silencing of NRR in rice showed that silencing the expression of NRRa and/or NRRb led to the insensitivity of the -P starvation response in rice seedling root growth, and displayed extra elongation of primary and adventitious roots compared to controls. Conversely, overexpression of NRRa in rice caused the inhibited root growth under regular growth conditions.These suggested that the expression of both NRRa and NRRb are important components of the response to the deprivation of N and P, acting as repressors of root growth.
 ===Evolution===

← Older revision		Revision as of 07:50, 27 May 2014
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	NRR plays an important role in the responses of rice root architecture to nutrients.Compared to the previously reported genes, such as LPI<ref name="ref2"/> and OsPHR2<ref name="ref3"/>, with response only to P deprivation, the transcripts of NRRa and NRRb act as new components to regulate the rice root architecture in response to N or P nutrient starvation.		NRR plays an important role in the responses of rice root architecture to nutrients.Compared to the previously reported genes, such as LPI<ref name="ref2"/> and OsPHR2<ref name="ref3"/>, with response only to P deprivation, the transcripts of NRRa and NRRb act as new components to regulate the rice root architecture in response to N or P nutrient starvation.
		+
		+	NRR responded to multi-nutrient deprivation stress. Further investigation of the down stream genes regulated by NRR will facilitate the understanding of the molecular mechanism of root development controlled by NRRin response to macronutrient.

	'''GO assignment(s):''' [http://www.ricedata.cn/ontology/ontology.aspx?db=gene&ta=GO:0048364 GO:0048364]		'''GO assignment(s):''' [http://www.ricedata.cn/ontology/ontology.aspx?db=gene&ta=GO:0048364 GO:0048364]

← Older revision		Revision as of 07:49, 27 May 2014
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	===Expression===		===Expression===
		+
		+	NRR expresses constitutively in rice tissues of root, stem, leaf,and panicle, and its expression level in seedling roots was enhanced under N, P, or NPK combined nutrient starvation.
		+
	Expression of NRR in rice seedling roots was significantly influenced by deficiency of macronutrients. Knock-down of expression of NRRa or NRRb by RNA interference resulted in enhanced rice root growth. By contrast, overexpression of NRRa in rice exhibited significantly retarded root growth. These results revealed that both NRRa and NRRb played negative regulatory roles in rice root growth.		Expression of NRR in rice seedling roots was significantly influenced by deficiency of macronutrients. Knock-down of expression of NRRa or NRRb by RNA interference resulted in enhanced rice root growth. By contrast, overexpression of NRRa in rice exhibited significantly retarded root growth. These results revealed that both NRRa and NRRb played negative regulatory roles in rice root growth.

@@ Line 28: / Line 28: @@
 ==References==
 <references>
-<ref name="ref1">Zhang, Yu-Man, et al.A novel rice gene, NRR responds to macronutrient deficiency and regulates root growth. Molecular plant 5.1 (2012): 63-72.
+<ref name="ref1">Zhang, Yu-Man, et al.A novel rice gene, NRR responds to macronutrient deficiency and regulates root growth. Molecular plant 5.1 (2012): 63-72.</ref>
-<ref name="ref2">Sa´nchez-Caldero´n, L., Lo´pez-Bucio, J., Chaco´n-Lo´pez, A., Gutie´rrezOrtega, A., Herna´ndez-Abreu, E., and Herrera-Estrella, L.(2006). Characterization of low phosphorus insensitive mutants reveals a crosstalk between low phosphorus-induced determinate root development and the activation of genes involved in the adaptation of Arabidopsis to phosphorus deficiency. Plant Physiol.140,879–889.
+<ref name="ref2">Sa´nchez-Caldero´n, L., Lo´pez-Bucio, J., Chaco´n-Lo´pez, A., Gutie´rrezOrtega, A., Herna´ndez-Abreu, E., and Herrera-Estrella, L.(2006). Characterization of low phosphorus insensitive mutants reveals a crosstalk between low phosphorus-induced determinate root development and the activation of genes involved in the adaptation of Arabidopsis to phosphorus deficiency. Plant Physiol.140,879–889.</ref>
-<ref name="ref3">Zhou, J., Jiao, F.C., Wu, Z., Li, Y., Wang, X., He, X., Zhong, W., and Wu, P.(2008).OsPHR2is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.Plant Physiol.146,1673–1686.
+<ref name="ref3">Zhou, J., Jiao, F.C., Wu, Z., Li, Y., Wang, X., He, X., Zhong, W., and Wu, P.(2008).OsPHR2is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants.Plant Physiol.146,1673–1686.</ref>
 </references>

@@ Line 6: / Line 6: @@
 NRR gene is a single-copy gene located on chromosome 5, which contains four exons and three introns embedded in the protein-coding region<ref name="ref1"/>.NRR encodes two kinds of transcripts that have the same 5'end but differ in the location of their 3'ends: the longer transcripts terminate at the 3'UTR within the fourth exon, whereas the 3'ends of the shorter transcripts are situated in the second intron at a region of 165–204 bp from the donor splicing site. We have designated the longer and shorter transcripts as NRRa and NRRb, respectively. NRRa is composed of all the four exons and encodes a protein (NRRa) with 308-aa residues. NRRb contains only the first two exons and produces a 223-aa protein (NRRb), with the last residue valine derived from the first three nucleotides of intron 2.
-Both NRRa and NRRb acting as the key components, modulate the rice root architecture with the availability of macronutrients.
+NRR plays an important role in the responses of rice root architecture to nutrients.Compared to the previously reported genes, such as LPI<ref name="ref2"/> and OsPHR2<ref name="ref3"/>, with response only to P deprivation, the transcripts of NRRa and NRRb act as new components to regulate the rice root architecture in response to N or P nutrient starvation.
 '''GO assignment(s):''' [http://www.ricedata.cn/ontology/ontology.aspx?db=gene&ta=GO:0048364 GO:0048364]

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 ===Function===
 [[File:Alternative splice.png|right|thumb|150px|"Schematic diagram of the generation of NRRa and NRRb from the primary NRR transcript.(from reference  <ref name="ref1" />)."]]
 NRR gene is a single-copy gene located on chromosome 5, which contains four exons and three introns embedded in the protein-coding region<ref name="ref1"/>.NRR encodes two kinds of transcripts that have the same 5'end but differ in the location of their 3'ends: the longer transcripts terminate at the 3'UTR within the fourth exon, whereas the 3'ends of the shorter transcripts are situated in the second intron at a region of 165–204 bp from the donor splicing site. We have designated the longer and shorter transcripts as NRRa and NRRb, respectively. NRRa is composed of all the four exons and encodes a protein (NRRa) with 308-aa residues. NRRb contains only the first two exons and produces a 223-aa protein (NRRb), with the last residue valine derived from the first three nucleotides of intron 2.