Os06g0110000 - Revision history

192.168.72.52: /* Structured Information */

2015-06-12T07:48:27Z

‎Structured Information

Gaojin: /* References */

2014-05-30T05:30:31Z

‎References

Gaojin: /* Evolution */

2014-05-30T05:30:07Z

‎Evolution

Guojian Wei at 15:33, 19 May 2014

2014-05-19T15:33:07Z

Guojian Wei at 15:32, 19 May 2014

2014-05-19T15:32:29Z

Guojian Wei at 12:33, 19 May 2014

2014-05-19T12:33:41Z

Guojian Wei at 12:32, 19 May 2014

2014-05-19T12:32:37Z

Guojian Wei at 12:32, 19 May 2014

2014-05-19T12:32:05Z

Guojian Wei at 12:30, 19 May 2014

2014-05-19T12:30:49Z

Guojian Wei at 12:26, 19 May 2014

2014-05-19T12:26:48Z

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 05:30, 30 May 2014
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	<ref name="ref3"> Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.</ref>		<ref name="ref3"> Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.</ref>
		+	<ref name="李家洋nature12870"> Jiang L, Liu X, Xiong G, et al. DWARF 53 acts as a repressor of strigolactone signalling in rice[J]. Nature 2013.</ref>
		+	<ref name="NATURE rice-1">Guo S, Xu Y, Liu H, et al. The interaction between OsMADS57 and OsTB1 modulates rice tillering via DWARF14[J]. Nature communications 2013; 4: 1566.</ref>
	</references>		</references>

← Older revision		Revision as of 05:30, 30 May 2014
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	[[File:5.jpg\|right\|thumb\|150px\|Figure 5 ]]		[[File:5.jpg\|right\|thumb\|150px\|Figure 5 ]]

		+	=== Knowledge Extension ===
		+	[[File:SLs signal patyway.jpg‎\|right\|thumb\|150px\|''A proposed model of Strigolactone(SL) signalling patyway <ref name="李家洋nature12870" />).'']]
		+	Strigolactones (SLs) are a group of newly identified plant hormones that control plant shoot branching<ref name="李家洋nature12870" />. SL signaling requires the hormone-dependent interaction of DWARF14 (D14) which is regulated by the interaction of OsMADS57 with OsTB1<ref name="NATURE rice-1" />.
		+	In this study<ref name="李家洋nature12870" />, they have identified theD53gene that encodes a substrate of the SCF(D3) ubiquitination complex, and revealed that D53 functions as a repressor ofSL signalling. These results allow to establish a model of SL signalling that is centred around a D14–D3–D53 signalling axis . In the presence of SLs, perception of SL by D14 and the SCF(D3) complex leads to ubiquitination of D53 and its subsequent degradation by the ubiquitin proteasome system, which in turn releases the repression of downstream target genes . In the d53 plant, the mutated D53 protein is resistant to ubiquitination and degradation, leading to the accumulation of d53, which blocks SL signalling and results in dwarf and high tillering phenotypes. The signalling paradigm of SLs is still emerging as SLs are a relatively new class of plant hormone for which many knowledge gaps still exist. Identification of D53 as a repressor of SL signalling adds a critical piece of information that helps to paint the whole picture of the SL signalling pathways.
		+	Moreover, the work has also provided an important paradigm for understanding signalling pathways of other plant hormones, for example, karrikins, a class of plant growth regulators found in the smoke of burning plants. Karrikin signalling involves MAX2 and KAI2, a D14-like α / β-hydrolase. It is probable that a similar protein to D53 could serve as the repressor of karrikin signalling. Indeed, multiple D53-like proteins are found in rice and inArabidopsis. We propose that these proteins could serve as repressors of signalling by karrikin and other plant hormones, in a similar way to D53 in SL signalling.<ref name="李家洋nature12870" />

	You can also add sub-section(s) at will.		You can also add sub-section(s) at will.

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 ===Expression===
 Tissue specificity of D3 expression was examined by RT–PCR analysis (Fig. 4A, B),the expression of D3 mRNA is regulated posttranscriptionaly or that transcripts with different lengths of 3′-untranslated regions are generated<ref name="ref1" />.The effect of d3 mutation on D3 mRNA expression was also analyzed by RT–PCR (Fig. 4C),it is strongly suggested that the function of the D3 gene is hampered in the Id3 mutant<ref name="ref1" />.The mRNA levels of D3 is increased during cell death by qRT-PCR analyzed.These results suggest that D3 protein is involved in leaf senescence or cell death.
 [[File:4.jpg]]
 Figure 4．Analysis of D3 expression. (A) Exon/intron structure of the D3 gene predicted on the basis of the cDNA sequence (AK065478) and cDNA regions amplified in RT–PCR analysis. D3 comprises four exons and three introns, and the predicted protein-coding region,shown as an open square, is present in the first exon. a–h represent the cDNA regions amplified in the RT–PCR analysis. A closed triangle represents the putative transposon insertion region in Id3. (B) Tissue specificity of D3 expression was examined by RT–PCR analysis using two sets of primers that amplify the b and d regions. L, leaves; R, roots; VM, vegetative meristems; RM, reproductive meristems. (C) Expression of D3 in the Id3 mutant. RNA samples isolated from young leaves were used for the RT–PCR analysis. S, wild-type Shiokari; Id3, Id3 mutant <ref name="ref1" />.

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 ===Expression===
 Tissue specificity of D3 expression was examined by RT–PCR analysis (Fig. 4A, B),the expression of D3 mRNA is regulated posttranscriptionaly or that transcripts with different lengths of 3′-untranslated regions are generated<ref name="ref1" />.The effect of d3 mutation on D3 mRNA expression was also analyzed by RT–PCR (Fig. 4C),it is strongly suggested that the function of the D3 gene is hampered in the Id3 mutant<ref name="ref1" />.The mRNA levels of D3 is increased during cell death by qRT-PCR analyzed.These results suggest that D3 protein is involved in leaf senescence or cell death.
-[[File:4.jpg|right|thumb|120px|Figure 4．Analysis of D3 expression. (A) Exon/intron structure of the D3 gene predicted on the basis of the cDNA sequence (AK065478) and cDNA regions amplified in RT–PCR analysis. D3 comprises four exons and three introns, and the predicted protein-coding region,shown as an open square, is present in the first exon. a–h represent the cDNA regions amplified in the RT–PCR analysis. A closed triangle represents the putative transposon insertion region in Id3. (B) Tissue specificity of D3 expression was examined by RT–PCR analysis using two sets of primers that amplify the b and d regions. L, leaves; R, roots; VM, vegetative meristems; RM, reproductive meristems. (C) Expression of D3 in the Id3 mutant. RNA samples isolated from young leaves were used for the RT–PCR analysis. S, wild-type Shiokari; Id3, Id3 mutant.(from reference <ref name="ref1" />).'']]
+[[File:4.jpg]]
 ===Evolution===

← Older revision		Revision as of 12:33, 19 May 2014
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	<ref name="ref3"> Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.</ref>		<ref name="ref3"> Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.</ref>
		+	</references>

	==Structured Information==		==Structured Information==

@@ Line 45: / Line 45: @@
 <ref name="ref3"> Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.</ref>
 ==Structured Information==
 {{JaponicaGene|

← Older revision		Revision as of 12:32, 19 May 2014
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	==References==		==References==
		+	<references>
	<ref name="ref1"> Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I,Kyozuka J. Suppression of tiller bud activity in tillering dwarf mutants of rice[J]. Plant and Cell Physiology, 2005, 46(1): 79-86.</ref>		<ref name="ref1"> Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I,Kyozuka J. Suppression of tiller bud activity in tillering dwarf mutants of rice[J]. Plant and Cell Physiology, 2005, 46(1): 79-86.</ref>

@@ Line 39: / Line 39: @@
 ==References==
-[1] Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I,Kyozuka J. Suppression of tiller bud activity in tillering dwarf mutants of rice[J]. Plant and Cell   Physiology, 2005, 46(1): 79-86.
+<ref name="ref1"> Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I,Kyozuka J. Suppression of tiller bud activity in tillering dwarf mutants of rice[J]. Plant and Cell   Physiology, 2005, 46(1): 79-86.</ref>
-[2] Yan H, Saika H, Maekawa M, Takamure I, Tsutsumi N,Kyozuka J, Nakazono M. Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death[J]. Genes & genetic systems, 2007, 82(4): 361-366.
+<ref name="ref2"> Yan H, Saika H, Maekawa M, Takamure I, Tsutsumi N,Kyozuka J, Nakazono M. Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death[J]. Genes & genetic systems, 2007, 82(4): 361-366.</ref>
-[3] Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.
+<ref name="ref3"> Yasuno N, Yasui Y, Takamure I, Kato K. Genetic interaction between 2 tillering genes, reduced culm number 1 (rcn1) and tillering dwarf gene d3, in rice [J]. Journal of heredity, 2007, 98(2): 169-172.</ref>
 ==Structured Information==
 {{JaponicaGene|

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 ===Evolution===
 The D3 gene sequenced and cloned by Ishikawa et al.A database search with the predicted D3 amino acid sequence indicated the presence of an N-terminal F-box and an LRR in the middle, suggesting that D3 is a member of the Fbox LRR family of proteins that function as a component of the ubiquitin E3 ligase complex(Figure5)<ref name="ref1" />.The D3 gene showed the highest sequence similarity to MAX2/ORE9 of Arabidopsis.The insertion of a putative transposon at the 154th amino acid of the predicted D3 protein caused an alteration of the amino acid sequence and generated a stop codon<ref name="ref2" />.
-[[File:5.jpg|right|thumb|150px|Figure 5(from reference <ref name="ref1" />).'']]
+[[File:5.jpg|right|thumb|150px|Figure 5 ]]