Os06g0125800 - Revision history

Rice2012: /* Structured Information */

2015-06-12T08:02:27Z

‎Structured Information

2014-06-09T00:58:21Z

‎Mutation

2014-06-09T00:56:46Z

‎Expression

2014-06-09T00:55:41Z

‎Function

2014-06-09T00:54:19Z

‎Function

2014-06-09T00:51:58Z

‎References

2014-06-09T00:51:42Z

‎Labs working on this gene

2014-06-09T00:51:23Z

‎Evolution

2014-06-09T00:50:21Z

‎Expression

2014-06-09T00:49:53Z

‎Function

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 The OsBBI1-overexpressing plants showed higher levels of H 2O2 accumulation in cells and higher levels of phenolic compounds and cross-linking of proteins in cell walls at infection sites byM. oryzaecompared with wild-type (WT) plants. The ell walls were thicker in the OsBBI1-overexpressing plants and thinner in the mutant plants than in the WT plants.
 We compared the disease lesion sizes on the OsBBI1-OE and WT plants after inoculation with traces of ZB1 (strain 2000-2) and ZB15 (strain 09-31-1). Lesion sizes on the OsBBI1-OE plants were significantly reduced compared with those on the WT plants, resulting in 50-80% and 45-50% reduction when infected by ZB1 and ZB15, respectively(figure3C).
 ===Localization===

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 . OsBBI1 encodes a RING protein with E3 ligase activity
-The OsBBI1 gene encodes a 261-aa protein, which contains a conserved RING domain at the  C-terminal end. To confirm whether the OsBBI1 protein has E3 Ubi ligase activity, we  expressed  full-length  OsBBI1  as  a  six  His-tagged fusion  protein  (His-OsBBI1)  in Escherichia  coli and purified the recombinant protein for enzyme activity assay (Figure 7).
+The OsBBI1 gene encodes a 261-aa protein, which contains a conserved RING domain at the  C-terminal end. To confirm whether the OsBBI1 protein has E3 Ubi ligase activity, we  expressed  full-length  OsBBI1  as  a  six  His-tagged fusion  protein  (His-OsBBI1)  in Escherichia  coli and purified the recombinant protein for enzyme activity assay.
 ===Mutation===

← Older revision		Revision as of 00:55, 9 June 2014
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−	[[File:figure7B 7C.jpg]] [[File:figure5B 5D 5E 5F.jpg]]	+	[[File:figure7B 7C.jpg]]
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		+	[[File:figure5B 5D 5E 5F.jpg]]

	===Expression===		===Expression===

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 .Enhancement of cell wall defence response in OsBBI1 overexpression plants
 The percentages of autofluorescent cell walls and cross-linking increased by ~10% in the OsBBI1-OE plants compared with that in the WT plants at 24 h.p.i. This increase was much more significant at 48 h.p.i. (Figure 5B and 5D). In contrast, the percentages of autofluorescent cell walls and cross-linking of wall-associated proteins at infection sites were lower in the osbbi1 plants than those in the WT plants at 24 and 48 h.p.i. (Figure 5E and 5F).
 ===Expression===

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 ==References==
-Please input cited references here.
+.Wei Li, Sihui Zhong, Guojun et al.(2011) ,LiRice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence,Cell Research (2011) 21:835-848.
 ==Structured Information==

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 ==Labs working on this gene==
-Please input related labs here.
+.National Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China;
 ==References==

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 The OsBBI1 gene encodes a 261-aa protein, which contains a conserved RING domain at the  C-terminal end. To confirm whether the OsBBI1 protein has E3 Ubi ligase activity, we  expressed  full-length  OsBBI1  as  a  six  His-tagged fusion  protein  (His-OsBBI1)  in Escherichia  coli and purified the recombinant protein for enzyme activity assay (Figure 7).
-===Evolution===
+===Mutation===
-Please input evolution information here.
+.Mutation in OsBBI1 leads to increased susceptibility to M. oryzae
-You can also add sub-section(s) at will.
+.Overexpression of OsBBI1 leads to broad-spectrum resistance against M. oryzae
 ==Labs working on this gene==

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 ===Expression===
-Please input expression information here.
+. OsBBI1 is induced by BTH and M. oryzae
 ===Evolution===

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 ==Annotated Information==
 ===Function===
-Please input function information here.
+.OsBBI1 modulates broad-spectrum resistance to blast fungus by modifying cell wall defence responses. Disease lesions on the osbbi1 leaves were significantly larger than on the WT leaves after inoculation with two races of M. oryzae. These results indicate that OsBBI1 is required for full immunity in rice against M. oryzae.
 ===Expression===