Os06g0139000 - Revision history

Rice2012: /* Structured Information */

2015-06-12T08:05:56Z

‎Structured Information

Lilly3850: /* summary */

2014-05-15T01:44:59Z

‎summary

Lilly3850: /* summary */

2014-05-15T01:44:42Z

‎summary

Lilly3850: /* Annotated Information */

2014-05-15T01:43:21Z

‎Annotated Information

Lilly3850: /* Annotated Information */

2014-05-15T01:41:25Z

‎Annotated Information

Lilly3850: /* introduction */

2014-05-15T01:40:29Z

‎introduction

Yangmei li: /* Function */

2014-05-14T05:45:03Z

‎Function

Yangmei li: /* Function */

2014-05-14T05:44:47Z

‎Function

Lilly3850: /* Annotated Information */

2014-05-14T03:04:03Z

‎Annotated Information

Lilly3850: /* Expression */

2014-05-13T11:08:57Z

‎Expression

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===summary===
 DDF1 controls organ size by regulating both cell division and cell expansion. In theddf1-1 spikelet, the specification of floral organs in whorls 2 and 3 is altered, with most lodicules and stamens being transformed into glume-like organs and pistil-like organs, respectively, but the specification of lemma/ palea and pistil in whorls 1 and 4 is not affected. DDF1 encodes an F-box protein anchored in the nucleolus, and is expressed in almost all vegetative and reproductive tissues. Consistent with the mutant floral phenotype, DDF1 positively regulates B-class genes OsMADS4 and OsMADS16, and negatively regulates pistil specification gene DL. In addition, DDF1 also negatively regulates the Arabidopsis LFY ortholog APO2, implying a functional connection between DDF1 and APO2. Collectively, these results revealed that DDF1, as a newly identified F-box gene, is a crucial genetic factor with pleiotropic functions for both vegetative growth and floral
-organ specification in rice.<ref name="ref1" /><ref name="ref2" />
+organ specification in rice.<ref name="ref1" /><ref name="ref3" />
 ==References==

@@ Line 34: / Line 34: @@
 ===summary===
 DDF1 controls organ size by regulating both cell division and cell expansion. In theddf1-1 spikelet, the specification of floral organs in whorls 2 and 3 is altered, with most lodicules and stamens being transformed into glume-like organs and pistil-like organs, respectively, but the specification of lemma/ palea and pistil in whorls 1 and 4 is not affected. DDF1 encodes an F-box protein anchored in the nucleolus, and is expressed in almost all vegetative and reproductive tissues. Consistent with the mutant floral phenotype, DDF1 positively regulates B-class genes OsMADS4 and OsMADS16, and negatively regulates pistil specification gene DL. In addition, DDF1 also negatively regulates the Arabidopsis LFY ortholog APO2, implying a functional connection between DDF1 and APO2. Collectively, these results revealed that DDF1, as a newly identified F-box gene, is a crucial genetic factor with pleiotropic functions for both vegetative growth and floral
-organ specification in rice.
+organ specification in rice.<ref name="ref1" /><ref name="ref2" />
 ==References==

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 *Real-time quantitative PCR
 *mRNA in situ hybridization
 ==References==

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 ==Annotated Information==
-===Introduction===<ref name="ref1" />.
+===Introduction===
-F-box proteins are characterized by a conserved ‘F-box’ motif consisting of approximately 50 amino acids at the N terminus (Bai et al., 1994; Kipreos and Pagano, 2000). Most F-box proteins reported so far have been identified as components of the Skp, Cullin, F-box-containing (SCF) complex that functions in defining substrate specificity in the ubiquitin–proteasome pathway. Several other F-box proteins with function in non-SCF complex have also been found (Clifford et al., 2000; Galan et al., 2001). F-box genes belong to a large family in plants, containing 42 groups, each of which has a distinct domain organization (Xu et al.,2009). Studies have indicated that F-box genes play crucial roles in a number of biological processes in plants, including flower development, self-incompatibility, hormone response, circadian clock and photomorphogenesis,senescence, defense response, embryogenesis and seedling development (Moon et al., 2004;.
+F-box proteins are characterized by a conserved ‘F-box’ motif consisting of approximately 50 amino acids at the N terminus (Bai et al., 1994; Kipreos and Pagano, 2000). Most F-box proteins reported so far have been identified as components of the Skp, Cullin, F-box-containing (SCF) complex that functions in defining substrate specificity in the ubiquitin–proteasome pathway. Several other F-box proteins with function in non-SCF complex have also been found (Clifford et al., 2000; Galan et al., 2001). F-box genes belong to a large family in plants, containing 42 groups, each of which has a distinct domain organization (Xu et al.,2009). Studies have indicated that F-box genes play crucial roles in a number of biological processes in plants, including flower development, self-incompatibility, hormone response, circadian clock and photomorphogenesis,senescence, defense response, embryogenesis and seedling development (Moon et al., 2004;.<ref name="ref1" />.
-'''GO assignment(s):''' Oryza sativa L., dwarf and deformed flower 1, F-box, vegetative growth, floral organ development
+'''Keywords:''' Oryza sativa L., dwarf and deformed flower 1, F-box, vegetative growth, floral organ development
 ===Function===

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 [[File:pair3_tupian2.jpg|right|thumb|275px|'''Figure 2.''' '' Histological characterization of the leaf, root and stem of ddf1-1 plants .(from reference)<ref name="ref1" />).'']]
 controlling monocot vegetative and reproductive development ddf1-1 exhibits significant size reduction in the whole plant, except the spikelet.Cell division and cell expansion are inhibited in ddf1-1. <ref name="ref1" />The expression of cell division/expansion-related genes is downregulated in ddf1-1.ddf1-1 also shows significant structural abnormalities in florets.<ref name="ref3" />The ddf1-1 phenotype is caused by a single nucleotide substitution.DDF1 encodes an LRR-type F-box protein, anchored in nucleolus.The expression pattern of DDF1 is consistent with the phenotypes of ddf1-1.DDF1 regulates the expression of several floral organ specification genes<ref name="ref2" />.DDF1 encodes an LRR-type F-box protein, anchored in nucleolus.The expression pattern of DDF1 is consistent with the phenotypes of ddf1-1.DDF1 regulates the expression of several floral organ specification genes.
 ===Expression===

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 ===Expression===
+[[File:pair3_11.jpg|right|thumb|275px|'''Figure 3.''' '' ddf1-1, all the mutant phenotypes (including dwarf stature,smaller stems, leaves, roots and panicles, and aberrant spikelet) were rescued (Figure 6d,e). Thus, the DDF1 gene was confirmed. .(from reference)<ref name="ref1" />).'']]
-The mutant ddf1-1 was discovered from a breeding population.The ddf1-1 plant is much shorter and thinner than the wild type, and this difference is distinct at the early seedling stage and becomes more apparent as the plants develop. The final plant height of the mutant reaches only about half of that of the wild type (Figure 1a–e and Figure S1)<ref name="ref2" /><ref name="ref1" />. All vegetative organs in ddf1-1, including stems<ref name="ref1" />, internodes, leaves and roots (both fibrous and lateral), are significantly shorter and thinner than in the wild type (Figure 1f–j and Figures S2 and S3). In addition, the ddf1-1 panicle is also notably shorter and smaller,<ref name="ref1" /> consisting of fewer primary and secondary branches and spikelets (Figure 1e,f; Figure S4). These phenotypes suggest that plant growth in ddf1-1 is seriously stunted. However, the size of the spikelet<ref name="ref1" />, the numbers of various vegetative organs<ref name="ref1" /><ref name="ref2" /> (e.g. internodes and tillers; Figure S5) and the time from sowing to heading (Figure 1e) remain unaffected in ddf1-1.
+ discovered from a breeding population.The ddf1-1 plant is much shorter and thinner than the wild type, and this difference is distinct at the early seedling stage and becomes more apparent as the plants develop. The final plant height of the mutant reaches only about half of that of the wild type (Figure 1a–e and Figure S1)<ref name="ref2" /><ref name="ref1" />. All vegetative organs in ddf1-1, including stems<ref name="ref1" />, internodes, leaves and roots (both fibrous and lateral), are significantly shorter and thinner than in the wild type (Figure 1f–j and Figures S2 and S3). In addition, the ddf1-1 panicle is also notably shorter and smaller,<ref name="ref1" /> consisting of fewer primary and secondary branches and spikelets (Figure 1e,f; Figure S4). These phenotypes suggest that plant growth in ddf1-1 is seriously stunted. However, the size of the spikelet<ref name="ref1" />, the numbers of various vegetative organs<ref name="ref1" /><ref name="ref2" /> (e.g. internodes and tillers; Figure S5) and the time from sowing to heading (Figure 1e) remain unaffected in ddf1-1.
 ===Evolution===