Os06g0160700 - Revision history

Rice2012: /* Structured Information */

2015-06-12T08:07:14Z

‎Structured Information

Liumengm: /* Labs working on this gene */

2014-06-07T07:59:50Z

‎Labs working on this gene

Liumengm: /* References */

2014-06-07T07:52:18Z

‎References

Liumengm: /* References */

2014-06-07T07:46:08Z

‎References

Liumengm: /* Evolution */

2014-06-07T07:43:03Z

‎Evolution

Liumengm: /* Expression */

2014-06-07T07:42:22Z

‎Expression

Liumengm: /* Expression */

2014-06-07T07:42:00Z

‎Expression

Liumengm: /* Expression */

2014-06-07T07:41:11Z

‎Expression

Liumengm: /* Structured Information */

2014-06-07T07:25:47Z

‎Structured Information

Liumengm: /* References */

2014-06-07T07:25:25Z

‎References

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ==Labs working on this gene==
 Please input related labs here.
 ==References==

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 Function and Characterization of Starch Synthase I Using Mutants in Rice.  Plant Physiology, 2006, 140(3): 1070-1084
 Compensation and interaction between RISBZ1 and RPBF during grain filling in rice.The Plant Journal, 2009, 59(6): 908-920
 Structure, Organization, and Chromosomal Location of the Gene Encoding a Form of Rice Soluble Starch Synthase  Plant Physiology, 1995, 108(2): 677-683
 ==Structured Information==

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 Please input cited references here.
 Function and Characterization of Starch Synthase I Using Mutants in Rice.  Plant Physiology, 2006, 140(3): 1070-1084
 ==Structured Information==

← Older revision		Revision as of 07:43, 7 June 2014
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	You can also add sub-section(s) at will.		You can also add sub-section(s) at will.
		+	A rice (Oryza sativa 1.) genomic clone encoding the gene for a form of soluble starch synthase (SSS1) and its 5’- and 3’-flanking regions has been isolated and sequenced. The SSSl gene contained 15 exons interrupted by 14 introns. The exon/intron organization of the SSSl gene was divergent from that of the rice Waxygene coding for granule-bound starch synthase, thus suggesting that the SSSl and granule-bound starch synthase genes have evolved from an ancestral gene in a different way or that the two genes are products of different ancestral genes that have converged during evolution. However, these two genes were closely located to each other on
		+	rice chromosome 6 at an approximate map distance of 5 centimorgans. The nucleotide sequence of the 5’-end region of the gene is unique because of the presence of some repetitive sequences.

	==Labs working on this gene==		==Labs working on this gene==

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 ===Expression===
 Please input expression information here.
-  In rice, there are 10 SS isoforms separated into five types; two granule-bound starch synthase (GBSS) isoforms (GBSSI and GBSSII) in the GBSS type, one SSI isoform in the SSI type, three SSII isoforms (SSIIa [SSII-3], SSIIb [SSII-2], and SSIIc [SSII-1]) in the SSII type, two SSIII isoforms (SSIIIa [SSIII-2] and SSIIIb [SSIII-1]) in the SSIII type, and two SSIV isoforms (SSIVa [SSIV-1] and SSIVb [SSIV-2]) in the SSIV type. Soluble fraction from rice developing endosperm was separated by anion-exchange chromatography with a linear gradient of 0 to 0.5 M NaCl. One peak of SS activity that eluted at 0.2 to 0.25 M of NaCl was detected in the presence of 0.5 M citrate and the absence of exogenous primers, whereas two SS activity peaks were detected at about 0.2 to 0.25 M and 0.35 M, in the absence of citrate but in the presence of glycogen primer. As in maize, SSI in rice exhibited SS activity even in the absence of exogenous primers, while rice SSIIIa activity was dependent on the exogenous primer (Fig. 1A). Native-PAGE/SS activity staining of each fraction from the HitrapQ column in gel containing rice amylopectin indicated that the strong activity middle band found in fraction numbers 3 to 5 corresponds to SSI activity.
+In rice, there are 10 SS isoforms separated into five types; two granule-bound starch synthase (GBSS) isoforms (GBSSI and GBSSII) in the GBSS type, one SSI isoform in the SSI type, three SSII isoforms (SSIIa [SSII-3], SSIIb [SSII-2], and SSIIc [SSII-1]) in the SSII type, two SSIII isoforms (SSIIIa [SSIII-2] and SSIIIb [SSIII-1]) in the SSIII type, and two SSIV isoforms (SSIVa [SSIV-1] and SSIVb [SSIV-2]) in the SSIV type. Soluble fraction from rice developing endosperm was separated by anion-exchange chromatography with a linear gradient of 0 to 0.5 M NaCl. One peak of SS activity that eluted at 0.2 to 0.25 M of NaCl was detected in the presence of 0.5 M citrate and the absence of exogenous primers, whereas two SS activity peaks were detected at about 0.2 to 0.25 M and 0.35 M, in the absence of citrate but in the presence of glycogen primer. As in maize, SSI in rice exhibited SS activity even in the absence of exogenous primers, while rice SSIIIa activity was dependent on the exogenous primer (Fig. 1A). Native-PAGE/SS activity staining of each fraction from the HitrapQ column in gel containing rice amylopectin indicated that the strong activity middle band found in fraction numbers 3 to 5 corresponds to SSI activity.
-  Extensive searches for starch synthase genes were done in the databases of both the whole genome and full-length cDNAs of rice, and ten genes were revealed to comprise the starch synthase gene family. Multi-sequence alignment analysis of the starch synthase proteins from rice and other plant species suggested that they were grouped into five classes, soluble starch synthase I (SSI), SSII, SSIII, SSIV and granule-bound starch synthase (GBSS). In rice, there was one gene for SSI, three for SSII and two each for SSIII, IV and GBSS. The expression pattern of the ten genes in the developing caryopsis was examined by semiquantitative RT–PCR analysis. Based on the temporal expression patterns, the ten genes could be divided into three groups: (i) early expressers (SSII-2, III-1, GBSSII), which are expressed in the early stage of grain filling; (ii) late expressers (SSII-3, III-2, GBSSI), which are expressed in the mid to later stage of grain filling; and (iii) steady expressers (SSI, II-1, IV-1, IV-2), which are expressed relatively constantly during grain filling. Within a caryopsis, the three gene groups spatially share their expression, i.e. ‘‘early expressers’’ in the pericarp, the “late expressers’’ in the “endosperm’’ and the ‘‘steady expressers’’ in both tissues. In addition, this grouping was reflected in the expression pattern of various rice tissues: expression in non-endosperm, endosperm or all tissues examined. The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed.
+Extensive searches for starch synthase genes were done in the databases of both the whole genome and full-length cDNAs of rice, and ten genes were revealed to comprise the starch synthase gene family. Multi-sequence alignment analysis of the starch synthase proteins from rice and other plant species suggested that they were grouped into five classes, soluble starch synthase I (SSI), SSII, SSIII, SSIV and granule-bound starch synthase (GBSS). In rice, there was one gene for SSI, three for SSII and two each for SSIII, IV and GBSS. The expression pattern of the ten genes in the developing caryopsis was examined by semiquantitative RT–PCR analysis. Based on the temporal expression patterns, the ten genes could be divided into three groups: (i) early expressers (SSII-2, III-1, GBSSII), which are expressed in the early stage of grain filling; (ii) late expressers (SSII-3, III-2, GBSSI), which are expressed in the mid to later stage of grain filling; and (iii) steady expressers (SSI, II-1, IV-1, IV-2), which are expressed relatively constantly during grain filling. Within a caryopsis, the three gene groups spatially share their expression, i.e. ‘‘early expressers’’ in the pericarp, the “late expressers’’ in the “endosperm’’ and the ‘‘steady expressers’’ in both tissues. In addition, this grouping was reflected in the expression pattern of various rice tissues: expression in non-endosperm, endosperm or all tissues examined. The implications in this spatio-temporal work sharing of starch synthesis isogenes are discussed.
 ===Evolution===

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              Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP
              clade; Ehrhartoideae; Oryzeae; Oryza.
-Function and Characterization of Starch Synthase I Using Mutants in Rice.  Plant Physiology, 2006, 140(3): 1070-1084|
 Chromosome = [[:category:Japonica Chromosome 6|Chromosome 6]]|
 AP = Chromosome 6:3078297..3085809|