Os06g0211200 - Revision history

Chenxi Sang at 05:53, 22 October 2016

2016-10-22T05:53:28Z

Chenxi Sang at 05:53, 22 October 2016

2016-10-22T05:53:03Z

Chenxi Sang at 05:51, 22 October 2016

2016-10-22T05:51:40Z

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Rice2012: /* Structured Information */

2015-06-12T08:09:31Z

‎Structured Information

Lxzlxz at 09:35, 9 June 2014

2014-06-09T09:35:22Z

Lxzlxz at 09:34, 9 June 2014

2014-06-09T09:34:37Z

Lxzlxz at 09:33, 9 June 2014

2014-06-09T09:33:37Z

Lxzlxz at 09:31, 9 June 2014

2014-06-09T09:31:12Z

Wangweihong at 09:28, 9 June 2014

2014-06-09T09:28:22Z

Liuq036 at 09:22, 9 June 2014

2014-06-09T09:22:12Z

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 </ref>
 </references>
 ==Structured Information==

← Older revision		Revision as of 05:53, 22 October 2016
Line 1:		Line 1:
−	~~OsAREB1,an ABRE-binding protein responding to ABA and glucosemay, may function~~ as ~~a positive regulator~~ in ~~drought/heat stresses response,~~	+	The rice '''''Os06g0211200''''' was reported as '''''OsbZIP46''''' in 2012 <ref name="ref1" /> by researchers from China.
−	~~but a negative regulator in flowering time in Arabidopsis~~<ref name="~~refA~~" />.

	==Annotated Information==		==Annotated Information==

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 Resultsfor details). (C) Stress-dependent phosphorylation of the recombinant
 AREB1b polypeptide. Protein extracts prepared from T87 cells treated for 30
-min with 50�M ABA (Ab), 0.5 M NaCl (Na), and 0.8 M mannitol (Os, high
+min with 50/M ABA (Ab), 0.5 M NaCl (Na), and 0.8 M mannitol (Os, high
 osmolality) and at low temperature (Lt, 4°C) or untreated (Ct) were used for
 in-gel kinase activity assay. The recombinant AREB1b polypeptide was used as

@@ Line 140: / Line 140: @@
 without ABA treatment. We also discuss the activation mechanism
 of AREB1 by ABA-dependent phosphorylation in plants.
 Fig. 2 shows the properties of AREB1 protein fragment phosphorylation.
 [[File:Fig. 2. Properties of AREB1 protein fragment phosphorylation.jpg]]
 You can also add sub-section(s) at will.

@@ Line 115: / Line 115: @@
 Kanget al. (17) reported that overexpression of ABF3 and
-ABF4�AREB2 resulted in ABA-hypersensitive phenotypes in
+ABF4/AREB2 resulted in ABA-hypersensitive phenotypes in
 germination and seedling growth stages in Arabidopsis. These
 transgenic plants also showed improvement of drought stress
-tolerance, suggesting that AREB�ABF proteins are involved in
+tolerance, suggesting that AREB/ABF proteins are involved in
 ABA response and stress tolerance in plants. However, AREB1
 and AREB2 require ABA for their maximum activation, as shown
@@ Line 125: / Line 125: @@
 ABA-responsive 42-kDa kinase activities phosphorylate conserved
 regions of AREBs, suggesting that ABA-dependent phosphorylation may be involved in activation of the AREB subfamily proteins
-(7). Phosphorylation�dephosphorylation-regulated events were reported to play important roles in ABA signaling; SNF1-related
+(7). Phosphorylation/dephosphorylation-regulated events were reported to play important roles in ABA signaling; SNF1-related
 protein kinase homologues, ABA-activated protein kinase
-(AAPK) inVicia faba(18), and SRK2E�OST1 inArabidopsis(19,
+(AAPK) inVicia faba(18), and SRK2E/OST1 inArabidopsis(19,
 ) modulate ABA-dependent stomatal closure.ABI1andABI2,of
 which dominant-negative mutation causes ABA-insensitive mutantsabi1andabi2, encode a type 2C protein phosphatase (4, 5, 21).
@@ Line 133: / Line 133: @@
 hypersensitivity, ABI1 and ABI2 negatively regulate ABAdependent responses (22).
 Here, we report that the ABA-activated 42-kDa kinase activity
-phosphorylates Ser�Thr residues in the conserved R-X-X-S�Tsites
+phosphorylates Ser/Thr residues in the conserved R-X-X-S/Tsites
-of AREB1. Amino acid substitution of the Ser�Thr residues to Ala
+of AREB1. Amino acid substitution of the Ser/Thr residues to Ala
 and Asp resulted in suppression and high transactivation activity,
-respectively. A phosphorylated active form of AREB1 was obtained by substitution of Ser�Thr to Asp in all conserved R-X-XS�T sites. We show that transgenic plants overexpressing the
+respectively. A phosphorylated active form of AREB1 was obtained by substitution of Ser/Thr to Asp in all conserved R-X-XS/T sites. We show that transgenic plants overexpressing the
 phosphorylated active form AREB1 express not only ABA inducible genes, such as RD29B, but also seed-specific gene
 without ABA treatment. We also discuss the activation mechanism
 of AREB1 by ABA-dependent phosphorylation in plants.
 You can also add sub-section(s) at will.
@@ Line 165: / Line 168: @@
 , Kazuo Nakashima1, Kazuo Shinozaki2and Kazuko Yamaguchi-Shinozaki1,3*(2012) Toward understanding transcriptional regulatorynetworks in abiotic stress responses and tolerance in rice  Rice 2012,5:6
-<ref name="refD" /> Takashi Furihata*, Kyonoshin Maruyama*, Yasunari Fujita*, Taishi Umezawa†‡, Riichiro Yoshida†, Kazuo Shinozaki†‡§,and Kazuko Yamaguchi-Shinozaki*§¶(2005) Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1.PNAS � February 7, 2006 � vol. 103 � no. 6
+<ref name="refD" /> Takashi Furihata*, Kyonoshin Maruyama*, Yasunari Fujita*, Taishi Umezawa†‡, Riichiro Yoshida†, Kazuo Shinozaki†‡§,and Kazuko Yamaguchi-Shinozaki*§¶(2005) Abscisic acid-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1.PNAS  February 7, 2006  vol. 103 no. 6
 <ref name="refB" />Jin XF1, Xiong AS, Peng RH, Liu JG, Gao F, Chen JM, Yao QH. (2010) OsAREB1, an ABRE-binding protein responding to ABA and glucose, has multiple functions in Arabidopsis.BMB Rep 43(1):34-9.

@@ Line 111: / Line 111: @@
 was up-regulated by ABA treatment (Hobo et al. 1999,).
 TRAB1 is phosphorylated rapidly in response to ABA treatment (Kagaya et al. 2002).
 You can also add sub-section(s) at will.

@@ Line 21: / Line 21: @@
 under osmotic stress conditions
-bZIP-type transcription factors AREBs�ABFs bind an abscisic acid
+Also,in ''Arabidopsis'', bZIP-type transcription factors AREBs/ABFs bind an abscisic acid
 (ABA)-responsive cis-acting element named ABRE and transactivate downstream gene expression inArabidopsis. Because AREB1
 overexpression could not induce downstream gene expression,
 activation of AREB1 requires ABA-dependent posttranscriptional
 modification. We confirmed that ABA activated 42-kDa kinase
-activity, which, in turn, phosphorylated Ser�Thr residues of R-XX-S�T sites in the conserved regions of AREB1. Amino acid substitutions of R-X-X-S�T sites to Ala suppressed transactivation activity, and multiple substitution of these sites resulted in almost
+activity, which, in turn, phosphorylated Ser/Thr residues of R-XX-S/T sites in the conserved regions of AREB1. Amino acid substitutions of R-X-X-S/T sites to Ala suppressed transactivation activity, and multiple substitution of these sites resulted in almost
-complete suppression of transactivation activity in transient assays. In contrast, substitution of the Ser�Thr residues to Asp
+complete suppression of transactivation activity in transient assays. In contrast, substitution of the Ser/Thr residues to Asp
 resulted in high transactivation activity without exogenous ABA
 application. A phosphorylated, transcriptionally active form was
-achieved by substitution of Ser�Thr in all conserved R-X-X-S�T sites
+achieved by substitution of Ser/Thr in all conserved R-X-X-S/T sites
 to Asp. Transgenic plants overexpressing the phosphorylated active form of AREB1 expressed many ABA-inducible genes, such as
 RD29B, without ABA treatment. These results indicate that the
@@ Line 43: / Line 43: @@
 products of which may function in dehydration tolerance in both
 vegetative tissues and seeds. Many ABA-inducible genes contain a
-conserved element named ABA-responsive element (ABRE) (PyACGTGG�TC) in their promoter regions. The ABRE functions as
+conserved element named ABA-responsive element (ABRE) (PyACGTGG/TC) in their promoter regions. The ABRE functions as
 a cis-acting element and is involved in ABA-responsive gene expression.Each AREB protein
 contained a single bZIP-type DNA-binding domain, and expression