Os06g0213100 - Revision history

Liman: /* Expression */

2016-12-21T14:58:32Z

‎Expression

Liman: /* Labs working on this gene */

2016-12-21T14:57:54Z

‎Labs working on this gene

Liman at 14:57, 21 December 2016

2016-12-21T14:57:27Z

Liman at 14:45, 21 December 2016

2016-12-21T14:45:09Z

Liman at 09:08, 10 December 2016

2016-12-10T09:08:38Z

192.168.72.52 at 10:25, 16 June 2015

2015-06-16T10:25:26Z

Zqlyf: /* Transformation */

2014-05-29T08:32:03Z

‎Transformation

Zqlyf: /* Transformation */

2014-05-29T08:31:48Z

‎Transformation

Zqlyf: /* Labs working on this gene */

2014-05-29T08:27:44Z

‎Labs working on this gene

Zqlyf: /* Reference */

2014-05-29T08:27:03Z

‎Reference

@@ Line 13: / Line 13: @@
 ===Expression ===
-* Expression of '''''S5'''''' is extremely low throughout the life cycle, only at the background level in almost all of the stages.
+* Expression of '''''S5''''' is extremely low throughout the life cycle, only at the background level in almost all of the stages.
 ===Sublocalization===

@@ Line 24: / Line 24: @@
 *National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;
 *Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China;
-*Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; ¶National Center of Crop Design, China Bioway Biotech Group, Beijing 100085, China;
+*Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China;
-*College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; and **National Maize Improvement Center, China Agriculture University, Beijing 100094, China
+* National Center of Crop Design, China Bioway Biotech Group, Beijing 100085, China;
 ==References==
 <references>

@@ Line 6: / Line 6: @@
 ===Function===
-[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
+* '''''S5''''' encodes an aspartic protease conditioning embryo-sac fertility.
-===Mutation===
+[[File:Os06g0213100-1.png|center|thumb|400px|'''Fig. 1. Fertility of ORF5-NJ11 transgenic-positive and -negative plants with Balilla as the recipient.''' '' <ref name="ref1" />.'']]
 ===Expression ===
-[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
+* Expression of '''''S5'''''' is extremely low throughout the life cycle, only at the background level in almost all of the stages.
 ===Evolution===
@@ Line 22: / Line 25: @@
 *Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China;
 *Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; ¶National Center of Crop Design, China Bioway Biotech Group, Beijing 100085, China;
-*�College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; and **National Maize Improvement Center, China Agriculture University, Beijing 100094, China
+*College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China; and **National Maize Improvement Center, China Agriculture University, Beijing 100094, China
 ==References==
 <references>

@@ Line 1: / Line 1: @@
-The rice gene  was reported as '''''''''' in 20<ref name="ref1" /> by researchers from China and Japan.
+The rice gene Os06g0213100 was reported as '''''S5''''' in 2008<ref name="ref1" /> by researchers from China.
 ==Annotated Information==
 ===Gene Symbol===
-*''''' <=>
+*'''''Os06g0213100 <=> S5,OsS5
 ===Function===
@@ Line 19: / Line 19: @@
 You can also add sub-section(s) at will.
 ==Labs working on this gene==
+*National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China;
 ==References==
 <references>
 * <ref name="ref1">
+Chen J, Ding J, Ouyang Y, et al. A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica–japonica hybrids in rice[J]. Proceedings of the National Academy of Sciences, 2008, 105(32): 11436-11441.
 </ref>
 </references>

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+==Annotated Information==
 ===Function===
-Please input function information here.
+[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
 ===Evolution===
 You can also add sub-section(s) at will.
 ==Labs working on this gene==
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==
 		 [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 06]]

@@ Line 23: / Line 23: @@
 ==Transformation==
 Sequence polymorphisms with predictable functional changes among the genotypes in the predicted proteins were observed in ORF3 and ORF4 but not ORF1 or ORF2 (fig. S1). By investigating the transcripts, we observed that the translation start codons of ORF4 and ORF5 were located only 0.8 kb away, but the genes were transcribed in opposite directions. The ORF4 sequence of Balilla and 02428 was predicted to encode a protein with a transmembrane domain and had no homology with any known proteins (fig. S2). An 11–base pair (bp) deletion predicted to cause premature termination of the predicted protein and a loss of the putative transmembrane domain (fig. S2) was detected in ORF4 of Nanjing 11 and Dular relative to Balilla and 02428 (fig. S1). ORF3 was mapped 11.7 kb away from ORF4 and showed homology to a heat shock protein Hsp70 gene. The ORF3 sequences of Balilla and Dular have a 13-bp deletion relative to the other two genotypes (fig. S1), which results in a frameshift in the C terminus of the protein (fig. S3). On the basis of the sequence differences in these ORFs (fig. S1), we designated the ORF3 allele from Nanjing 11 and 02428 as ORF3+ and the other allele as ORF3–; the ORF4 allele from Balilla and 02428 as ORF4+ and the other one as ORF4–; and the ORF5 allele from Nanjing 11 as ORF5+, the one from Balilla as ORF5–, and those from Dular and 02428 as ORF5n[8].
 [[File:zq6.png]]
 Fig.2. Schematic representation of the killer-protector system in an indicajaponica hybrid regulated by the S5 locus. (A) A genetic model depicting the process of megaspore formation and effects of the three genes, where 3+, 3–, 4+, 4–, 5+, and 5– represent ORF3+, ORF3–, ORF4+, ORF4–, ORF5+, and ORF5–, respectively, and colored blocks and circles represent the proteins. In the megaspore mother cell and daughter cells immediately after meiotic division, killing would not occur because of the presence of ORF3+. Killing would occur in the daughter cell carrying ORF3– and ORF4+ at a later stage of megaspore development. (B) Hypothetical molecular processes involving ER-stress and PCD. bZIP50-S, spliced bZIP50; ER, endoplasmic reticulum; PM, plasma membrane.

@@ Line 28: / Line 28: @@
 ==Labs working on this gene==
 National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University
 School of Life Sciences, Chinese University of Hong Kong
-National Maize Improvement Center of China, China Agricultural University,
 Key Laboratory of Plant Functional Genomics, Ministry of Education of China
 Jiangsu Key Laboratory of Crop Genetics and Physiology
 Yangzhou University
 Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193, People’s Republic of China (M.X.)

@@ Line 43: / Line 43: @@
 [4]. R. Alcázar, A. V. García, J. E. Parker, M. Reymond, Proc. Natl. Acad. Sci. U.S.A. 106, 334 (2009).
 [5]. K. Bomblies, Annu. Rev. Plant Biol. 61, 109 (2010).
 [6].Y. Yamagata et al., Proc. Natl. Acad. Sci. U.S.A. 107, 1494 (2010).
 [7]. Delimitation of the rice wide compatibility gene S5n to a 40-kb DNA fragment .  Theoretical and Applied Genetics, 2005, 111(6): 1080-1086
-[8]. A Killer-Protector System Regulates Both Hybrid Sterility and Segregation Distortion in Rice . Science, 2012, 337(6100): 1336-1340
+[7]. Delimitation of the rice wide compatibility gene S5n to a 40-kb DNA fragment .  Theoretical and Applied Genetics, 2005, 111(6): 1080-1086.