Os06g0229800 - Revision history

Rice2012: /* Structured Information */

2015-06-12T08:11:55Z

‎Structured Information

2014-06-08T13:37:54Z

‎Evolution

2014-06-08T13:34:49Z

‎Function

2014-06-08T13:31:55Z

‎Function

2014-06-08T13:17:41Z

‎Annotated Information

2014-06-08T13:15:41Z

‎Annotated Information

2014-06-08T12:53:30Z

‎Annotated Information

2014-06-08T12:50:08Z

‎Annotated Information

2014-06-08T12:49:31Z

‎Annotated Information

2014-06-08T12:40:52Z

‎Expression

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 [[File:Phylogenetic trees of SSⅡ from 5 different plant species.jpg]]
-Figure:Phylogenetic trees of SSⅡ from 5 different plant species(The number values represent the branch length)
+Fig.4. Phylogenetic trees of SSⅡ from 5 different plant species(The number values represent the branch length)
 ==Labs working on this gene==

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 [[File:comparison.jpg]]
-Comparison between the conserved DNA fragments of ALK in 9 rice varieties. The different nucleotides are shown inbold. Numbers above the plus sign indicate the nucleotide position fromATG in ALK of Shuangke Zao.
+Fig.3. Comparison between the conserved DNA fragments of ALK in 9 rice varieties. The different nucleotides are shown inbold. Numbers above the plus sign indicate the nucleotide position fromATG in ALK of Shuangke Zao.
 alk cause the differences between a japonica rice variety Nipponbare and an indica rice variety Kasalath in terms of the disintegration of endosperm starch granules in alkali solution and their gelatinization in a 4 M urea solution, respectively, cosegregated in backcross inbred lines derived from a cross between the two varieties[1].

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 ===Function===
 ALK gene encodes a soluble starch synthase Ⅱ, controlling the temperature of rice pasting .The encoding amino acid product of  ALK gene may cause a change in starch synthase activity, thus affecting the synthesis of amylopectin branch chain of medium length, so that the crystal layer structure changes, resulting in the final performance of pasting temperature change.
 alk cause the differences between a japonica rice variety Nipponbare and an indica rice variety Kasalath in terms of the disintegration of endosperm starch granules in alkali solution and their gelatinization in a 4 M urea solution, respectively, cosegregated in backcross inbred lines derived from a cross between the two varieties[1].

← Older revision		Revision as of 13:17, 8 June 2014
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	This gene,which has been proposed to be involved in the biosynthesis of amylopectin.It is a single copy gene via Southern blot analysis.The product of this gene only have one ORF, which belongs to glycosyl transferase family. Genomic DNA contains seven introns and eight exons. full-length of cDNA is 2409bp,and the enzyme protein composed of 803 amino acids.		This gene,which has been proposed to be involved in the biosynthesis of amylopectin.It is a single copy gene via Southern blot analysis.The product of this gene only have one ORF, which belongs to glycosyl transferase family. Genomic DNA contains seven introns and eight exons. full-length of cDNA is 2409bp,and the enzyme protein composed of 803 amino acids.

		+	[[File:cDNA of alk.jpg]]
		+
		+	Fig.2. Demonstration of genome sequence and cDNA sequence of alk

	===Function===		===Function===

← Older revision		Revision as of 13:15, 8 June 2014
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	Fig.1. Genetic map of chromosome 6 in rice		Fig.1. Genetic map of chromosome 6 in rice
		+
		+	This gene,which has been proposed to be involved in the biosynthesis of amylopectin.It is a single copy gene via Southern blot analysis.The product of this gene only have one ORF, which belongs to glycosyl transferase family. Genomic DNA contains seven introns and eight exons. full-length of cDNA is 2409bp,and the enzyme protein composed of 803 amino acids.
		+

	===Function===		===Function===

← Older revision		Revision as of 12:53, 8 June 2014
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	ALK (alkali degeneration gene) ,located on chromosome 6,previous studies have demonstrated that acl(t), a determinator of the profile for amylopectin chain-length of subspecies Japonica and Indica, alk(t)and SSSIIa were all mapped to the same locus.		ALK (alkali degeneration gene) ,located on chromosome 6,previous studies have demonstrated that acl(t), a determinator of the profile for amylopectin chain-length of subspecies Japonica and Indica, alk(t)and SSSIIa were all mapped to the same locus.

		+	[[File:map location.jpg]]
		+
		+	Fig.1. Genetic map of chromosome 6 in rice

	===Function===		===Function===

← Older revision		Revision as of 12:50, 8 June 2014
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	==Annotated Information==		==Annotated Information==
−	ALK (alkali degeneration gene) ,located on chromosome 6,previous studies have demonstrated that acl(t), a determinator of the profile for amylopectin chain-length of subspecies Japonica and Indica, alk(t)and SSSIIa were all mapped to the same locus.	+	ALK (alkali degeneration gene) ,located on chromosome 6,previous studies have demonstrated that acl(t), a determinator of the profile for amylopectin chain-length of subspecies Japonica and Indica, alk(t)and SSSIIa were all mapped to the same locus.

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 ===Expression===
-ALK gene is a single copy gene, and it has a specific expression in the seed of rice,RT-PCR results showed that expression of the gene product is not detected in the blade tissues.
+ALK gene is a single copy gene, RT-PCR analysis demonstrated that ALK is expressed only in rice seeds,expression of the gene product is not detected in the blade tissues. In addition, no difference was detected at transcriptional level (fig. 5) among the rice varieties with high and low GT, suggesting that the alterations at the SSSII in different rice varieties may mainly affect its enzymatic activities.
 ===Evolution===