Os06g0597000 - Revision history

Chengtao: /* Function */

2014-05-19T14:42:06Z

‎Function

Jiaohzh: /* Evolution */

2014-05-19T14:38:19Z

‎Evolution

Chengtao: /* Evolution */

2014-05-19T14:38:01Z

‎Evolution

Lymzzu2009 at 14:30, 19 May 2014

2014-05-19T14:30:39Z

Chengtao: /* Expression */

2014-05-19T14:25:56Z

‎Expression

Sw at 14:23, 19 May 2014

2014-05-19T14:23:44Z

Chengtao: /* Function */

2014-05-19T14:21:58Z

‎Function

Lymzzu2009 at 14:20, 19 May 2014

2014-05-19T14:20:17Z

Chengtao: /* Function */

2014-05-19T14:18:53Z

‎Function

Jiaohzh: /* Function */

2014-05-19T14:17:51Z

‎Function

← Older revision		Revision as of 14:42, 19 May 2014
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	This gene, ''OsIAA23'', is a mutation of ''Aux/IAA'' gene in rice. The mutant is characterized by a loss of QC identity during postembryonic development, and the displayed defects result from a stabilizing mutation in domain II of OsIAA23 (Os06g39590).		This gene, ''OsIAA23'', is a mutation of ''Aux/IAA'' gene in rice. The mutant is characterized by a loss of QC identity during postembryonic development, and the displayed defects result from a stabilizing mutation in domain II of OsIAA23 (Os06g39590).
		+
	(1)Characterization of the mutant is show in '''Figure 1'''.		(1)Characterization of the mutant is show in '''Figure 1'''.

@@ Line 70: / Line 70: @@
 and lines, respectively. The numbers 0, 1, and 2 represent phase 0, 1,
 and 2 introns, respectively
 ==Labs working on this gene==

@@ Line 70: / Line 70: @@
 and lines, respectively. The numbers 0, 1, and 2 represent phase 0, 1,
 and 2 introns, respectively
 ==Labs working on this gene==

← Older revision		Revision as of 14:30, 19 May 2014
Line 40:		Line 40:
	(4) Reduction of auxin signaling mediated by ''OsIAA23'' is important for crown root primordia development.		(4) Reduction of auxin signaling mediated by ''OsIAA23'' is important for crown root primordia development.
	(5) ''OsIAA23'' does not act only in the QC, but auxin signaling mediated by ''OsIAA23'' in the QC plays a unique role in QC maintenance<ref name="pmid:1"/>.		(5) ''OsIAA23'' does not act only in the QC, but auxin signaling mediated by ''OsIAA23'' in the QC plays a unique role in QC maintenance<ref name="pmid:1"/>.
		+
		+	===Expression===
		+
		+	''OsIAA23'' was found to be constitutively expressed in all of the examined tissues, although a lower abundance was observed in the embryo '''(Figure 4)'''.
		+
		+	[[File:Figure_4._Expression_pattern_of_OsIAA23_revealed_by_a_GUS_reporter_line.jpg]]
		+
		+	Figure 4. Expression pattern of OsIAA23 revealed by a GUS reporter line
		+	(a, b) Stereomicroscope images showing GUS staining in the primary root tip (a) and the lateral root (LR) (b). Bar = 200 lm.
		+	(c) Vertical section of the root tip of the transgenic plants.
		+	(d, e) Cross section at the matured root zone with the initiation of LR primordia of the transgenic plants and the magnified view of the stele (e). Bar = 20 lm.
		+	(f, g) OsIAA23 (f) and QHB (g) expression during the process of LR primordia development. Bar = 20 lm.
		+	(h–j) Cross-sections at the shoot base. (h), (i) and (j) are different stages of crown root primordia. Bar = 100 lm.

← Older revision		Revision as of 14:25, 19 May 2014
Line 40:		Line 40:
	(4) Reduction of auxin signaling mediated by ''OsIAA23'' is important for crown root primordia development.		(4) Reduction of auxin signaling mediated by ''OsIAA23'' is important for crown root primordia development.
	(5) ''OsIAA23'' does not act only in the QC, but auxin signaling mediated by ''OsIAA23'' in the QC plays a unique role in QC maintenance<ref name="pmid:1"/>.		(5) ''OsIAA23'' does not act only in the QC, but auxin signaling mediated by ''OsIAA23'' in the QC plays a unique role in QC maintenance<ref name="pmid:1"/>.
−
−
−	~~===Expression===~~
−
−	~~''OsIAA23'' was found to be constitutively expressed in all of the examined tissues, although a lower abundance was observed in the embryo '''(Figure 4)'''.~~
−
−	~~[[File:Figure_4._Expression_pattern_of_OsIAA23_revealed_by_a_GUS_reporter_line.jpg]]~~
−
−	~~Figure 4. Expression pattern of OsIAA23 revealed by a GUS reporter line~~
−	~~(a, b) Stereomicroscope images showing GUS staining in the primary root tip (a) and the lateral root (LR) (b). Bar = 200 lm.~~
−	~~(c) Vertical section of the root tip of the transgenic plants.~~
−	~~(d, e) Cross section at the matured root zone with the initiation of LR primordia of the transgenic plants and the magnified view of the stele (e). Bar = 20 lm.~~
−	~~(f, g) OsIAA23 (f) and QHB (g) expression during the process of LR primordia development. Bar = 20 lm.~~
−	~~(h–j) Cross-sections at the shoot base. (h), (i) and (j) are different stages of crown root primordia. Bar = 100 lm.~~

← Older revision		Revision as of 14:23, 19 May 2014
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	Figure 2. Map-based cloning and complementation test of OsIAA23.		Figure 2. Map-based cloning and complementation test of OsIAA23.
		+
		+	(3) Response to auxin is reduced in ''Osiaa23''
		+	To investigate the auxin response of the ''Osiaa23'' mutant,WT and ''Osiaa23'' mutants were treated with a-naphthalene acetic acid (NAA). These results indicated a reduced sensitivity to auxin in the ''Osiaa23'' mutant '''(Figure 3)'''.
		+
		+	[[File:Figure_3._Alteration_of_auxin_response_in_the_Osiaa23_mutant.jpg‎]]
		+
		+	Figure 3. Alteration of auxin response in the Osiaa23 mutant.
		+	(a) Root growth performance of the wild type (WT; left) and Osiaa23 (right) plants grown in a solution culture with 1 lM NAA for 7 days. Bar = 1 cm.
		+	(b) Proliferation of root hairs at the root apex of WT (left) and Osiaa23 (right) induced by 0.1 lM NAA treatment. Bar = 0.5 mm.
		+	(c, d) Cross-sections indicate DR5p::GUS expression in the root zone with the initiation of lateral root (LR) primordia of WT (c) and Osiaa23 (d). Bars = 20 lm.
		+	(e, f) GUS staining of cross-sections at the shoot base with the initiation of crown root primordial in WT (e) and Osiaa23 (f). Bar = 0.5 mm.
		+	(g, h) GUS staining of the root apex of 1 d-old seedlings of WT and Osiaa23. Bars = 100 lm.
		+
		+
		+	(4) Reduction of auxin signaling mediated by ''OsIAA23'' is important for crown root primordia development.
		+	(5) ''OsIAA23'' does not act only in the QC, but auxin signaling mediated by ''OsIAA23'' in the QC plays a unique role in QC maintenance<ref name="pmid:1"/>.
		+

	===Expression===		===Expression===

← Older revision		Revision as of 14:21, 19 May 2014
Line 24:		Line 24:

	Figure 2. Map-based cloning and complementation test of OsIAA23.		Figure 2. Map-based cloning and complementation test of OsIAA23.
−
−	~~(3) Response to auxin is reduced in ''Osiaa23''~~
−	To investigate the auxin response of the ''Osiaa23'' mutant,WT and ''Osiaa23'' mutants were treated with a-naphthalene acetic acid (NAA). These results indicated a reduced sensitivity to auxin in the ''Osiaa23'' mutant '''(Figure 3)'''.
−
−	~~[[File:Figure_3._Alteration_of_auxin_response_in_the_Osiaa23_mutant.jpg‎]]~~
−
−	~~Figure 3. Alteration of auxin response in the Osiaa23 mutant.~~
−	~~(a) Root growth performance of the wild type (WT; left) and Osiaa23 (right) plants grown in a solution culture with 1 lM NAA for 7 days. Bar = 1 cm.~~
−	~~(b) Proliferation of root hairs at the root apex of WT (left) and Osiaa23 (right) induced by 0.1 lM NAA treatment. Bar = 0.5 mm.~~
−	~~(c, d) Cross-sections indicate DR5p::GUS expression in the root zone with the initiation of lateral root (LR) primordia of WT (c) and Osiaa23 (d). Bars = 20 lm.~~
−	~~(e, f) GUS staining of cross-sections at the shoot base with the initiation of crown root primordial in WT (e) and Osiaa23 (f). Bar = 0.5 mm.~~
−	~~(g, h) GUS staining of the root apex of 1 d-old seedlings of WT and Osiaa23. Bars = 100 lm.~~
−
−
−	~~(4) Reduction of auxin signaling mediated by ''OsIAA23'' is important for crown root primordia development.~~
−	~~(5) ''OsIAA23'' does not act only in the QC, but auxin signaling mediated by ''OsIAA23'' in the QC plays a unique role in QC maintenance<ref name="pmid:1"/>.~~

	===Expression===		===Expression===

← Older revision		Revision as of 14:20, 19 May 2014
Line 16:		Line 16:

	(a) A point mutation was detected in the mutant, which resulted in a G to A base pair change of an Aux/IAA gene (OsIAA23), causing a change of glycine (G) to glutamate (E) in the conserved core sequence of domain II. The exons of OsIAA23 are indicated by thick rectangles, and introns are indicated by thin lines. The four domain positions are indicated by black boxes. (b) Confirmation of the point mutation in OsIAA23 using the dCAPS marker. From left to right: the WT, the heterozygous OsIAA23, and the homozygous OsIAA23, respectively. (c) Two transgenic plants containing the OsIAA23 gene exhibited phenotypes characteristic of the heterozygous and homozygous OsIAA23.		(a) A point mutation was detected in the mutant, which resulted in a G to A base pair change of an Aux/IAA gene (OsIAA23), causing a change of glycine (G) to glutamate (E) in the conserved core sequence of domain II. The exons of OsIAA23 are indicated by thick rectangles, and introns are indicated by thin lines. The four domain positions are indicated by black boxes. (b) Confirmation of the point mutation in OsIAA23 using the dCAPS marker. From left to right: the WT, the heterozygous OsIAA23, and the homozygous OsIAA23, respectively. (c) Two transgenic plants containing the OsIAA23 gene exhibited phenotypes characteristic of the heterozygous and homozygous OsIAA23.
		+
		+	(2) Cloning of the ''OsIAA23'' gene
		+	Heterozygous ''Osiaa23'' (''japonica'') was crossed with Kasalath (''indica''). Approximately half of the F1 population that exhibited the heterozygous mutant phenotype was selected and self-pollinated. The derived F2 population was used to map ''OsIAA23''.
		+	The ''OsIAA23'' gene, sequenced from homozygous mutant genomic DNA, was found to contain a G to A base pair substitution causing a glycine (G) to glutamate (E) change (Gly64Glu) in domain II of ''OsIAA23'' '''(Figure 2)'''. These results confirm that the root tissue defects in the mutant are caused by the point mutation in domain II of ''OsIAA23''.
		+
		+	[[File:Figure_2._Map-based_cloning_and_complementation_test_of_OsIAA23.jpg]]
		+
		+	Figure 2. Map-based cloning and complementation test of OsIAA23.

	(3) Response to auxin is reduced in ''Osiaa23''		(3) Response to auxin is reduced in ''Osiaa23''

← Older revision		Revision as of 14:18, 19 May 2014
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	(a) Root growth of 5-day-old seedlings of the wild type (WT) (left) and the heterozygous (middle) and homozygous mutant (right). Bar = 2 cm. (b–d) Cross-sections at the stem base of the WT (b), heterozygous mutant (c), and homozygous mutant (d). Bar = 0.5 mm. (e) Plants at the grain filling stage. From left to right: WT, the heterozygous mutant, and the homozygous mutant. (f) The panicle of three genotypes. From left to right: WT, the heterozygous mutant, and the homozygous mutant. Bar = 2 cm. (g–i) Stereomicroscopic images of OsCYCB1;1p::GUS in the roots of the three genotypes. From left to right: WT, the heterozygous mutant, and the homozygous mutant. Bar = 0.5 mm. (j) Gravitropic response of three genotypes. Seedlings were grown for 5 days under g1 gravitropic conditions and then moved to g2 where they were grown for 2 additional days. From top to bottom: WT, the heterozygous mutant, and the homozygous mutant. Bar = 2 cm.		(a) Root growth of 5-day-old seedlings of the wild type (WT) (left) and the heterozygous (middle) and homozygous mutant (right). Bar = 2 cm. (b–d) Cross-sections at the stem base of the WT (b), heterozygous mutant (c), and homozygous mutant (d). Bar = 0.5 mm. (e) Plants at the grain filling stage. From left to right: WT, the heterozygous mutant, and the homozygous mutant. (f) The panicle of three genotypes. From left to right: WT, the heterozygous mutant, and the homozygous mutant. Bar = 2 cm. (g–i) Stereomicroscopic images of OsCYCB1;1p::GUS in the roots of the three genotypes. From left to right: WT, the heterozygous mutant, and the homozygous mutant. Bar = 0.5 mm. (j) Gravitropic response of three genotypes. Seedlings were grown for 5 days under g1 gravitropic conditions and then moved to g2 where they were grown for 2 additional days. From top to bottom: WT, the heterozygous mutant, and the homozygous mutant. Bar = 2 cm.

−	~~(2) Cloning of the ''OsIAA23'' gene~~
−	Heterozygous ''Osiaa23'' (''japonica'') was crossed with Kasalath (''indica''). Approximately half of the F1 population that exhibited the heterozygous mutant phenotype was selected and self-pollinated. The derived F2 population was used to map ''OsIAA23''.
−	The ''OsIAA23'' gene, sequenced from homozygous mutant genomic DNA, was found to contain a G to A base pair substitution causing a glycine (G) to glutamate (E) change (Gly64Glu) in domain II of ''OsIAA23'' '''(Figure 2)'''. These results confirm that the root tissue defects in the mutant are caused by the point mutation in domain II of ''OsIAA23''.

−	~~[[File:Figure_2._Map-based_cloning_and_complementation_test_of_OsIAA23.jpg]]~~
−
−	~~Figure 2. Map-based cloning and complementation test of OsIAA23.~~
	(a) A point mutation was detected in the mutant, which resulted in a G to A base pair change of an Aux/IAA gene (OsIAA23), causing a change of glycine (G) to glutamate (E) in the conserved core sequence of domain II. The exons of OsIAA23 are indicated by thick rectangles, and introns are indicated by thin lines. The four domain positions are indicated by black boxes. (b) Confirmation of the point mutation in OsIAA23 using the dCAPS marker. From left to right: the WT, the heterozygous OsIAA23, and the homozygous OsIAA23, respectively. (c) Two transgenic plants containing the OsIAA23 gene exhibited phenotypes characteristic of the heterozygous and homozygous OsIAA23.		(a) A point mutation was detected in the mutant, which resulted in a G to A base pair change of an Aux/IAA gene (OsIAA23), causing a change of glycine (G) to glutamate (E) in the conserved core sequence of domain II. The exons of OsIAA23 are indicated by thick rectangles, and introns are indicated by thin lines. The four domain positions are indicated by black boxes. (b) Confirmation of the point mutation in OsIAA23 using the dCAPS marker. From left to right: the WT, the heterozygous OsIAA23, and the homozygous OsIAA23, respectively. (c) Two transgenic plants containing the OsIAA23 gene exhibited phenotypes characteristic of the heterozygous and homozygous OsIAA23.

← Older revision		Revision as of 14:17, 19 May 2014
Line 6:		Line 6:


		+	This gene, ''OsIAA23'', is a mutation of ''Aux/IAA'' gene in rice. The mutant is characterized by a loss of QC identity during postembryonic development, and the displayed defects result from a stabilizing mutation in domain II of OsIAA23 (Os06g39590).
		+	(1)Characterization of the mutant is show in '''Figure 1'''.
		+
		+	[[File:Figure_1._Characterization_of_the_mutant.jpg‎]]
		+
		+	Figure 1. Characterization of the mutant.
		+	(a) Root growth of 5-day-old seedlings of the wild type (WT) (left) and the heterozygous (middle) and homozygous mutant (right). Bar = 2 cm. (b–d) Cross-sections at the stem base of the WT (b), heterozygous mutant (c), and homozygous mutant (d). Bar = 0.5 mm. (e) Plants at the grain filling stage. From left to right: WT, the heterozygous mutant, and the homozygous mutant. (f) The panicle of three genotypes. From left to right: WT, the heterozygous mutant, and the homozygous mutant. Bar = 2 cm. (g–i) Stereomicroscopic images of OsCYCB1;1p::GUS in the roots of the three genotypes. From left to right: WT, the heterozygous mutant, and the homozygous mutant. Bar = 0.5 mm. (j) Gravitropic response of three genotypes. Seedlings were grown for 5 days under g1 gravitropic conditions and then moved to g2 where they were grown for 2 additional days. From top to bottom: WT, the heterozygous mutant, and the homozygous mutant. Bar = 2 cm.

	(2) Cloning of the ''OsIAA23'' gene		(2) Cloning of the ''OsIAA23'' gene