Os06g0603000 - Revision history

Xuanxi: /* Labs working on this gene */

2014-06-09T09:03:06Z

‎Labs working on this gene

Xuanxi: /* Function */

2014-06-09T09:00:56Z

‎Function

Xuanxi: /* Function */

2014-06-09T09:00:26Z

‎Function

Xuanxi: /* Function */

2014-06-09T08:59:37Z

‎Function

Xuanxi: /* Function */

2014-06-09T08:58:39Z

‎Function

Xuanxi: /* Function */

2014-06-09T08:55:07Z

‎Function

Xuanxi: /* Function */

2014-06-09T08:52:15Z

‎Function

Xuanxi: /* Location and Expression */

2014-06-09T08:51:34Z

‎Location and Expression

Xuanxi: /* Location and Expression */

2014-06-09T08:50:40Z

‎Location and Expression

Xuanxi: /* Location and Expression */

2014-06-09T08:47:32Z

‎Location and Expression

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 . Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources, College of Horticulture and Landscape Architecture, Hainan University, Haikou, 570228, People’s Republic of China
-.Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Takayama, Ikoma, Nara 630-0101, Japan
+.Laboratory of Plant Molecular Genetics, Nara Institute of Science and Technology, Takayama, Ikoma, Nara 630-0101, Japan<ref name="ref3"/>
-.National Institute of Agrobiological Resources, Kannondai, Tsukuba, Ibaraki 305-8602, Japan
+.National Institute of Agrobiological Resources, Kannondai, Tsukuba, Ibaraki 305-8602, Japan<ref name="ref3"/>
-.Research Institute for Advanced Science and Technology, University of Osaka Prefecture, Sakai, Osaka 599-8570, Japan
+.Research Institute for Advanced Science and Technology, University of Osaka Prefecture, Sakai, Osaka 599-8570, Japan<ref name="ref3"/>
 ==References==

@@ Line 9: / Line 9: @@
 Subsequently, RNAi-1 and wild-type plants were selected for further analysis of the expression profiles of SE5 gene and corresponding protein levels in various tissues (Fig. 3e, f). Either SE5 transcripts or SE5 protein were highly expressed in stems and leaves in wild-type, but relatively less in roots and seeds. There was a higher level of SE5 protein in flowers compared to a low abundance of SE5 transcripts. However, the SE5 RNAi plants displayed decreased levels of both SE5 transcripts and SE5 protein. Additionally, except for relatively higher expression in root tissues, there was comparable expression of OsHO2 transcripts in either wild-type or RNAi-1 plants.
 In comparison with wild-type, the SE5 RNAi plants had weaker growth, with fewer stems and a yellowish color, when grown under LD conditions. For example, 50-day-old SE5 RNAi plants showed an apparent yellowish phenotype (Fig. 4a) and chlorophyll levels decreased to 47.9 and 54.4 % of the fifth and sixth leaves of wild-type, respectively, while corresponding chlorophyll a to b ratios were 14.5 and 14.0 % higher than those of wild-type plants (Fig. 4b).<ref name="ref2"/>
-[[File:xuanxixuanxi3.jpg]  [[File:xuanxixuanxi4.jpg]]
+[[File:xuanxixuanxi3.jpg]]  [[File:xuanxixuanxi4.jpg]]
 ===Location and Expression===

@@ Line 5: / Line 5: @@
 ===Function===
-PHOTOPERIOD SENSITIVITY 5 ( SE5 ) was first presumed to encode a rice HO with high similarity to Arabidopsis HY1, although enzyme activity of SE5 was not confirmed (Izawa et al. 2000). The se5 mutant has a very early flowering phenotype under both short-day and long-day (LD) conditions, and is completely deficient in photoperiodic response (Izawa et al. 2000).Previous pharmacological results have shown that hematin, an HO-1 inducer, could protect wheat leaves from oxidative damage triggered by paraquat and Peroxide (Sa et al. 2007).In the present study,they showed that the SE5 protein was located in the chloroplast at least, and exhibited HO activity. Subsequently, RNAi knockdown of the rice SE5 gene results in loss of SE5 mRNA and SE5 protein. We also demonstrated that com-pared with wild-type, SE5 RNAi plants were more sus-ceptible to MV treatment. As expected in a se5 mutant (Izawa et al. 2000), there was a similar phenotype of early flowering under LD conditions. By contrast, the addition of exogenous CO aqueous solution partially rescued the cor-responding MV hypersensitivity. Transgenic Arabidopsis plants overexpressing SE5 and HY1 were generated and their corresponding tolerance phenotypes in the presence of MV were characterized<ref name="ref2"/>.
+'''PHOTOPERIOD SENSITIVITY 5 ( SE5 )''' was first presumed to encode a rice HO with high similarity to Arabidopsis HY1, although enzyme activity of SE5 was not confirmed (Izawa et al. 2000). The se5 mutant has a very early flowering phenotype under both short-day and long-day (LD) conditions, and is completely deficient in photoperiodic response (Izawa et al. 2000).Previous pharmacological results have shown that hematin, an HO-1 inducer, could protect wheat leaves from oxidative damage triggered by paraquat and Peroxide (Sa et al. 2007).In the present study,they showed that the SE5 protein was located in the chloroplast at least, and exhibited HO activity. Subsequently, RNAi knockdown of the rice SE5 gene results in loss of SE5 mRNA and SE5 protein. We also demonstrated that com-pared with wild-type, SE5 RNAi plants were more sus-ceptible to MV treatment. As expected in a se5 mutant (Izawa et al. 2000), there was a similar phenotype of early flowering under LD conditions. By contrast, the addition of exogenous CO aqueous solution partially rescued the cor-responding MV hypersensitivity. Transgenic Arabidopsis plants overexpressing SE5 and HY1 were generated and their corresponding tolerance phenotypes in the presence of MV were characterized<ref name="ref2"/>.
 '''To investigate the physiological role of SE5 in rice''', they obtained two knockdown transgenic lines by an RNAi approach (Fig. 3a). T-DNA insertion of the two T0 transgenic lines (RNAi-1 and RNAi-2) was confirmed by PCR-based analysis (Fig. 3b). Gene-specific RT-PCR showed that about 90 % of SE5 transcripts were specifically decreased by SE5 RNAi, whereas transcripts of OsHO2 were not affected in T2 progeny of transgenic plants (Fig. 3c). The protein levels of SE5 determined by western blotting displayed similar decreasing tendencies (Fig. 3d). The above results clearly indicate that only the transcripts and protein levels of SE5 were specifically reduced in SE5 RNAi transgenic plants.<ref name="ref2"/>
 Subsequently, RNAi-1 and wild-type plants were selected for further analysis of the expression profiles of SE5 gene and corresponding protein levels in various tissues (Fig. 3e, f). Either SE5 transcripts or SE5 protein were highly expressed in stems and leaves in wild-type, but relatively less in roots and seeds. There was a higher level of SE5 protein in flowers compared to a low abundance of SE5 transcripts. However, the SE5 RNAi plants displayed decreased levels of both SE5 transcripts and SE5 protein. Additionally, except for relatively higher expression in root tissues, there was comparable expression of OsHO2 transcripts in either wild-type or RNAi-1 plants.
 In comparison with wild-type, the SE5 RNAi plants had weaker growth, with fewer stems and a yellowish color, when grown under LD conditions. For example, 50-day-old SE5 RNAi plants showed an apparent yellowish phenotype (Fig. 4a) and chlorophyll levels decreased to 47.9 and 54.4 % of the fifth and sixth leaves of wild-type, respectively, while corresponding chlorophyll a to b ratios were 14.5 and 14.0 % higher than those of wild-type plants (Fig. 4b).<ref name="ref2"/>
-[[File:xuanxixuanxi3.jpg]]
+[[File:xuanxixuanxi3.jpg]  [[File:xuanxixuanxi4.jpg]]
 ===Location and Expression===

@@ Line 9: / Line 9: @@
 Subsequently, RNAi-1 and wild-type plants were selected for further analysis of the expression profiles of SE5 gene and corresponding protein levels in various tissues (Fig. 3e, f). Either SE5 transcripts or SE5 protein were highly expressed in stems and leaves in wild-type, but relatively less in roots and seeds. There was a higher level of SE5 protein in flowers compared to a low abundance of SE5 transcripts. However, the SE5 RNAi plants displayed decreased levels of both SE5 transcripts and SE5 protein. Additionally, except for relatively higher expression in root tissues, there was comparable expression of OsHO2 transcripts in either wild-type or RNAi-1 plants.
 In comparison with wild-type, the SE5 RNAi plants had weaker growth, with fewer stems and a yellowish color, when grown under LD conditions. For example, 50-day-old SE5 RNAi plants showed an apparent yellowish phenotype (Fig. 4a) and chlorophyll levels decreased to 47.9 and 54.4 % of the fifth and sixth leaves of wild-type, respectively, while corresponding chlorophyll a to b ratios were 14.5 and 14.0 % higher than those of wild-type plants (Fig. 4b).<ref name="ref2"/>
-[[File:xuanxixuanxi3.jpg]][[File:xuanxixuanxi4.jpg]]
+[[File:xuanxixuanxi3.jpg]]
 ===Location and Expression===

@@ Line 7: / Line 7: @@
 PHOTOPERIOD SENSITIVITY 5 ( SE5 ) was first presumed to encode a rice HO with high similarity to Arabidopsis HY1, although enzyme activity of SE5 was not confirmed (Izawa et al. 2000). The se5 mutant has a very early flowering phenotype under both short-day and long-day (LD) conditions, and is completely deficient in photoperiodic response (Izawa et al. 2000).Previous pharmacological results have shown that hematin, an HO-1 inducer, could protect wheat leaves from oxidative damage triggered by paraquat and Peroxide (Sa et al. 2007).In the present study,they showed that the SE5 protein was located in the chloroplast at least, and exhibited HO activity. Subsequently, RNAi knockdown of the rice SE5 gene results in loss of SE5 mRNA and SE5 protein. We also demonstrated that com-pared with wild-type, SE5 RNAi plants were more sus-ceptible to MV treatment. As expected in a se5 mutant (Izawa et al. 2000), there was a similar phenotype of early flowering under LD conditions. By contrast, the addition of exogenous CO aqueous solution partially rescued the cor-responding MV hypersensitivity. Transgenic Arabidopsis plants overexpressing SE5 and HY1 were generated and their corresponding tolerance phenotypes in the presence of MV were characterized<ref name="ref2"/>.
 '''To investigate the physiological role of SE5 in rice''', they obtained two knockdown transgenic lines by an RNAi approach (Fig. 3a). T-DNA insertion of the two T0 transgenic lines (RNAi-1 and RNAi-2) was confirmed by PCR-based analysis (Fig. 3b). Gene-specific RT-PCR showed that about 90 % of SE5 transcripts were specifically decreased by SE5 RNAi, whereas transcripts of OsHO2 were not affected in T2 progeny of transgenic plants (Fig. 3c). The protein levels of SE5 determined by western blotting displayed similar decreasing tendencies (Fig. 3d). The above results clearly indicate that only the transcripts and protein levels of SE5 were specifically reduced in SE5 RNAi transgenic plants.<ref name="ref2"/>
 Subsequently, RNAi-1 and wild-type plants were selected for further analysis of the expression profiles of SE5 gene and corresponding protein levels in various tissues (Fig. 3e, f). Either SE5 transcripts or SE5 protein were highly expressed in stems and leaves in wild-type, but relatively less in roots and seeds. There was a higher level of SE5 protein in flowers compared to a low abundance of SE5 transcripts. However, the SE5 RNAi plants displayed decreased levels of both SE5 transcripts and SE5 protein. Additionally, except for relatively higher expression in root tissues, there was comparable expression of OsHO2 transcripts in either wild-type or RNAi-1 plants.
-In comparison with wild-type, the SE5 RNAi plants had weaker growth, with fewer stems and a yellowish color, when grown under LD conditions. For example, 50-day-old SE5 RNAi plants showed an apparent yellowish phenotype (Fig. 4a) and chlorophyll levels decreased to 47.9 and 54.4 % of the fifth and sixth leaves of wild-type, respectively, while corresponding chlorophyll a to b ratios were 14.5 and 14.0 % higher than those of wild-type plants (Fig. 4b).
+In comparison with wild-type, the SE5 RNAi plants had weaker growth, with fewer stems and a yellowish color, when grown under LD conditions. For example, 50-day-old SE5 RNAi plants showed an apparent yellowish phenotype (Fig. 4a) and chlorophyll levels decreased to 47.9 and 54.4 % of the fifth and sixth leaves of wild-type, respectively, while corresponding chlorophyll a to b ratios were 14.5 and 14.0 % higher than those of wild-type plants (Fig. 4b).<ref name="ref2"/>
-[[File:xuanxixuanxi4.jpg]]
+[[File:xuanxixuanxi3.jpg]][[File:xuanxixuanxi4.jpg]]
 ===Location and Expression===

← Older revision		Revision as of 08:51, 9 June 2014
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	[[File:xuanxixuanxi1.jpg]] [[File:xuanxixuanxi2.jpg]]		[[File:xuanxixuanxi1.jpg]] [[File:xuanxixuanxi2.jpg]]
−	To investigate the physiological role of SE5 in rice, they obtained two knockdown transgenic lines by an RNAi approach (Fig. 3a). T-DNA insertion of the two T0 transgenic lines (RNAi-1 and RNAi-2) was confirmed by PCR-based analysis (Fig. 3b). Gene-specific RT-PCR showed that about 90 % of SE5 transcripts were specifically decreased by SE5 RNAi, whereas transcripts of OsHO2 were not affected in T2 progeny of transgenic plants (Fig. 3c). The protein levels of SE5 determined by western blotting displayed similar decreasing tendencies (Fig. 3d). The above results clearly indicate that only the transcripts and protein levels of SE5 were specifically reduced in SE5 RNAi transgenic plants.<ref name="ref2"/>
−	~~[[File:xuanxixuanxi3.jpg]]~~

	===Investigation Proceeding===		===Investigation Proceeding===

← Older revision		Revision as of 08:50, 9 June 2014
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	[[File:xuanxixuanxi1.jpg]] [[File:xuanxixuanxi2.jpg]]		[[File:xuanxixuanxi1.jpg]] [[File:xuanxixuanxi2.jpg]]
		+	To investigate the physiological role of SE5 in rice, they obtained two knockdown transgenic lines by an RNAi approach (Fig. 3a). T-DNA insertion of the two T0 transgenic lines (RNAi-1 and RNAi-2) was confirmed by PCR-based analysis (Fig. 3b). Gene-specific RT-PCR showed that about 90 % of SE5 transcripts were specifically decreased by SE5 RNAi, whereas transcripts of OsHO2 were not affected in T2 progeny of transgenic plants (Fig. 3c). The protein levels of SE5 determined by western blotting displayed similar decreasing tendencies (Fig. 3d). The above results clearly indicate that only the transcripts and protein levels of SE5 were specifically reduced in SE5 RNAi transgenic plants.<ref name="ref2"/>
	[[File:xuanxixuanxi3.jpg]]		[[File:xuanxixuanxi3.jpg]]

← Older revision		Revision as of 08:47, 9 June 2014
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	[[File:xuanxixuanxi3.jpg]]		[[File:xuanxixuanxi3.jpg]]