Os06g0625900 - Revision history

Rice2012: /* Structured Information */

2015-06-12T08:20:08Z

‎Structured Information

Franky123: /* Expression */

2014-06-08T15:46:05Z

‎Expression

Franky123: /* Expression */

2014-06-08T15:44:20Z

‎Expression

Franky123: /* Expression */

2014-06-08T15:43:03Z

‎Expression

Franky123: /* Evolution */

2014-06-08T15:39:10Z

‎Evolution

Franky123: /* Expression */

2014-06-08T15:37:51Z

‎Expression

Franky123: /* References */

2014-06-08T15:27:59Z

‎References

Franky123: /* References */

2014-06-08T15:21:10Z

‎References

Franky123: /* Labs working on this gene */

2014-06-08T15:19:38Z

‎Labs working on this gene

Franky123: /* Labs working on this gene */

2014-06-08T15:16:36Z

‎Labs working on this gene

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 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

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 ===Expression===
 [[File:QQ图片20140608232107.jpg]]
 K+loss in the E. coli strain TKW4205 transformed with plasmid pBAD24 containing the OsHAK1-1, OsHAK7, or OsHAK10 cDNAs. Time courses of the K+ contents of cells induced in 13 mM Ara and suspended in K+-free minimal medium, pH 5.5, supplemented with 4.9% (w/v) sorbitol, and 10 mM propionic acid. Black circles, Cells transformed with pBAD24; white circles, cells transformed with OsHAK1-1; black triangles, cells transformed with OsHAK7; white triangles, cells transformed with OsHAK10
 [[File:QQ图片20140608232936.jpg]]
 Tonoplast localization of HAK10: GFP. Transient expressions in onion epidermal cells of a OsHAK10:GFP translational fusion protein was visualized by epifluorescence microscopy. A, GFP fluorescence concentrated to the tonoplast. The large vacuole of onion epidermal cells occupies most of the cell volume. B through D, Captions of different focal planes of the cell imaged in A, showing transvacuolar strands of cytoplasm (tvs). Note that the transvacuolar strands seen in C and D are lined by two tonoplast membranes. E, The tonoplast follows the cell contour except in the perinuclear region (n) where the vacuole detaches from the cell surface. F, Clear field caption of the cell shown in E depicting the perinuclear region (n). As controls, transient expression assays in onion epidermal cells of GFP and AtNHX1:GFP were carried out in parallel. G, In the control cell expressing GFP, the fluoresce concentrated in the cytoplasm and was manifestly absent in the vacuole. H, In the control cell expressing AtNHX1:GFP, the fluorescence concentrated to the tonoplast showing the perinuclear region (n). Bar = 25um. Please input expression information here.
 ===Evolution===

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 [[File:QQ图片20140608232936.jpg]]
-Tonoplast localization of HAK10: GFP. Transient expressions in onion epidermal cells of a OsHAK10:GFP translational fusion protein was visualized by epifluorescence microscopy. A, GFP fluorescence concentrated to the tonoplast. The large vacuole of onion epidermal cells occupies most of the cell volume. B through D, Captions of different focal planes of the cell imaged in A, showing transvacuolar strands of cytoplasm (tvs). Note that the transvacuolar strands seen in C and D are lined by two tonoplast membranes. E, The tonoplast follows the cell contour except in the perinuclear region (n) where the vacuole detaches from the cell surface. F, Clear field caption of the cell shown in E depicting the perinuclear region (n). As controls, transient expression assays in onion epidermal cells of GFP and AtNHX1:GFP were carried out in parallel. G, In the control cell expressing GFP, the fluoresce concentrated in the cytoplasm and was manifestly absent in the vacuole. H, In the control cell expressing AtNHX1:GFP, the fluorescence concentrated to the tonoplast showing the perinuclear region (n). Bar � 25 �m. Please input expression information here.
+Tonoplast localization of HAK10: GFP. Transient expressions in onion epidermal cells of a OsHAK10:GFP translational fusion protein was visualized by epifluorescence microscopy. A, GFP fluorescence concentrated to the tonoplast. The large vacuole of onion epidermal cells occupies most of the cell volume. B through D, Captions of different focal planes of the cell imaged in A, showing transvacuolar strands of cytoplasm (tvs). Note that the transvacuolar strands seen in C and D are lined by two tonoplast membranes. E, The tonoplast follows the cell contour except in the perinuclear region (n) where the vacuole detaches from the cell surface. F, Clear field caption of the cell shown in E depicting the perinuclear region (n). As controls, transient expression assays in onion epidermal cells of GFP and AtNHX1:GFP were carried out in parallel. G, In the control cell expressing GFP, the fluoresce concentrated in the cytoplasm and was manifestly absent in the vacuole. H, In the control cell expressing AtNHX1:GFP, the fluorescence concentrated to the tonoplast showing the perinuclear region (n). Bar = 25um. Please input expression information here.
 ===Evolution===

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 [[File:QQ图片20140608232107.jpg]]
-K� loss in the E. coli strain TKW4205 transformed with plasmid pBAD24 containing the OsHAK1-1, OsHAK7, or OsHAK10 cDNAs. Time courses of the K� contents of cells induced in 13 mM Ara and suspended in K�-free minimal medium, pH 5.5, supplemented with 4.9% (w/v) sorbitol, and 10 mM propionic acid. Black circles, Cells transformed with pBAD24; white circles, cells transformed with OsHAK1-1; black triangles, cells transformed with OsHAK7; white triangles, cells transformed with OsHAK10
+K+loss in the E. coli strain TKW4205 transformed with plasmid pBAD24 containing the OsHAK1-1, OsHAK7, or OsHAK10 cDNAs. Time courses of the K+ contents of cells induced in 13 mM Ara and suspended in K+-free minimal medium, pH 5.5, supplemented with 4.9% (w/v) sorbitol, and 10 mM propionic acid. Black circles, Cells transformed with pBAD24; white circles, cells transformed with OsHAK1-1; black triangles, cells transformed with OsHAK7; white triangles, cells transformed with OsHAK10
 Please input expression information here.
 ===Evolution===

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 ===Evolution===
-[[File:QQ图片20140608222722.jpg ]]
+[[File:QQ图片20140608232126.jpg ]]
 You can also add sub-section(s) at will.

← Older revision		Revision as of 15:37, 8 June 2014
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	===Expression===		===Expression===
		+	[[File:QQ图片20140608232107.jpg]]
		+
		+	K� loss in the E. coli strain TKW4205 transformed with plasmid pBAD24 containing the OsHAK1-1, OsHAK7, or OsHAK10 cDNAs. Time courses of the K� contents of cells induced in 13 mM Ara and suspended in K�-free minimal medium, pH 5.5, supplemented with 4.9% (w/v) sorbitol, and 10 mM propionic acid. Black circles, Cells transformed with pBAD24; white circles, cells transformed with OsHAK1-1; black triangles, cells transformed with OsHAK7; white triangles, cells transformed with OsHAK10
	Please input expression information here.		Please input expression information here.

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 Marı,, a A. Bañ, uelos, Blanca Garciadeblas, Beatriz Cubero, Alonso Rodrı,, guez-Navarro
 Plant Physiology, 2002, 130(2): 784-795
 '''Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice (Oryza sativa L.)
 '''Huan Wang; Meishan Zhang; Rui Guo; Decheng Shi; Bao Liu; Xiuyun Lin; Chunwu Yang

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 ==Labs working on this gene==
 （1）Department de Biotecnologı´a, Universidad Polite´cnica de Madrid, 28040 Madrid, Spain (M.A.B., B.G.,A.R.-N.); and Instituto de Recursos Naturales y Agrobiologı´a, Consejo Superior de Investigaciones Cientı´ficas, 41080 Sevilla, Spain (B.C.)
 （2）Key laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun 130024 Jilin Province, China

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 ==Labs working on this gene==
-（1）Departamento de Biotecnologı´a, Universidad Polite´cnica de Madrid, 28040 Madrid, Spain (M.A.B., B.G.,A.R.-N.); and Instituto de Recursos Naturales y Agrobiologı´a, Consejo Superior de Investigaciones Cientı´ficas, 41080 Sevilla, Spain (B.C.)
+（1）Department de Biotecnologı´a, Universidad Polite´cnica de Madrid, 28040 Madrid, Spain (M.A.B., B.G.,A.R.-N.); and Instituto de Recursos Naturales y Agrobiologı´a, Consejo Superior de Investigaciones Cientı´ficas, 41080 Sevilla, Spain (B.C.)
 ==References==