Os06g0660200 - Revision history

Xysj1980 at 15:39, 7 March 2017

2017-03-07T15:39:16Z

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Rice2012 at 08:21, 12 June 2015

2015-06-12T08:21:43Z

Zhdjsh at 14:38, 3 June 2014

2014-06-03T14:38:07Z

Ct at 14:32, 3 June 2014

2014-06-03T14:32:59Z

Ct at 14:05, 3 June 2014

2014-06-03T14:05:54Z

Hylwayne at 14:02, 3 June 2014

2014-06-03T14:02:48Z

Liuchongdj at 13:55, 3 June 2014

2014-06-03T13:55:06Z

Ct at 13:52, 3 June 2014

2014-06-03T13:52:42Z

Ct: /* Evolution */

2014-06-03T13:27:57Z

‎Evolution

Ct: /* Evolution */

2014-06-03T13:22:29Z

‎Evolution

@@ Line 159: / Line 159: @@
 ==Structured Information==
 [[Category:Genes]]
 [[Category:Japonica mRNA]]

← Older revision		Revision as of 14:38, 3 June 2014
Line 85:		Line 85:
	[[File:Phenotypes and molecular analyses ofOsPIN2over-expression transgenic plants (O1 and O2) and untranformed wild-type plant (WT).jpg]]		[[File:Phenotypes and molecular analyses ofOsPIN2over-expression transgenic plants (O1 and O2) and untranformed wild-type plant (WT).jpg]]

		+	'''Auxin transport altered byOsPIN2over-expression
		+	in rice'''
		+	To assess whether the over-expression ofOsPIN2affected the
		+	free auxin levels, the concentration of endogenous free IAA in
		+	various organs of the transgenic and WT plants was quantified
		+	by high-performance liquid chromatography (HPLC). In the first
		+	and second leaves from the top, O1 and O2 transgenic plants
		+	contained free IAA concentrations that were 11%–35% lower
		+	than that in the control plants (Figure 3a). In the sheath of the
		+	first leaf, the transgenic plants and WT had nearly the same
		+	concentration of free IAA, while in the sheath of the second
		+	leaf, O1 and O2 plants had 38%–53% lower free IAA concentration than that in WT (Figure 3b). In contrast, in the root–
		+	shoot junction (shoot base), the two transgenic lines contained
		+	65%–128% more free IAA than that in WT (Figure 3c).
		+	To detect the effect ofOsPIN2over-expression on auxin distribution, we separated individual roots into three segments:
		+	0–4 cm including the root apex and elongation zones; 4–8 cm,
		+	the lateral root area; and 8–12 cm which was adjacent to
		+	the root–shoot junction. Both transgenic and WT roots showed
		+	the same patterns of free IAA concentration with an abrupt
		+	decrease from the tip to the root base (Figure 3d).

		+	[[File:High-performance liquid chromatography analysis.jpg]]

	===Evolution===		===Evolution===

← Older revision		Revision as of 14:32, 3 June 2014
Line 85:		Line 85:
	[[File:Phenotypes and molecular analyses ofOsPIN2over-expression transgenic plants (O1 and O2) and untranformed wild-type plant (WT).jpg]]		[[File:Phenotypes and molecular analyses ofOsPIN2over-expression transgenic plants (O1 and O2) and untranformed wild-type plant (WT).jpg]]

−	~~'''Auxin transport altered byOsPIN2over-expression~~
−	~~in rice'''~~
−	~~To assess whether the over-expression ofOsPIN2affected the~~
−	~~free auxin levels, the concentration of endogenous free IAA in~~
−	~~various organs of the transgenic and WT plants was quantified~~
−	~~by high-performance liquid chromatography (HPLC). In the first~~
−	~~and second leaves from the top, O1 and O2 transgenic plants~~
−	~~contained free IAA concentrations that were 11%–35% lower~~
−	~~than that in the control plants (Figure 3a). In the sheath of the~~
−	~~first leaf, the transgenic plants and WT had nearly the same~~
−	~~concentration of free IAA, while in the sheath of the second~~
−	~~leaf, O1 and O2 plants had 38%–53% lower free IAA concentration than that in WT (Figure 3b). In contrast, in the root–~~
−	~~shoot junction (shoot base), the two transgenic lines contained~~
−	~~65%–128% more free IAA than that in WT (Figure 3c).~~
−	~~To detect the effect ofOsPIN2over-expression on auxin distribution, we separated individual roots into three segments:~~
−	~~0–4 cm including the root apex and elongation zones; 4–8 cm,~~
−	~~the lateral root area; and 8–12 cm which was adjacent to~~
−	~~the root–shoot junction. Both transgenic and WT roots showed~~
−	~~the same patterns of free IAA concentration with an abrupt~~
−	~~decrease from the tip to the root base (Figure 3d).~~

−	~~[[File:High-performance liquid chromatography analysis.jpg]]~~

	===Evolution===		===Evolution===

@@ Line 105: / Line 105: @@
 the same patterns of free IAA concentration with an abrupt
 decrease from the tip to the root base (Figure 3d).
 ===Evolution===

← Older revision		Revision as of 14:02, 3 June 2014
Line 50:		Line 50:
	OsPIN2transcriptional expression driven by pUbi in these tissues		OsPIN2transcriptional expression driven by pUbi in these tissues
	reached by 3–15 times.		reached by 3–15 times.
		+
		+	Over-expression ofOsPIN2significantly increased tiller angle
		+	and numbers and decreased height of the rice in comparison
		+	with WT (Figure 1i). At 80 days after germination,
		+	O1 and O2 plants had a wider tiller angle (the angle between
		+	the outermost tillers in the left and right side) (44° and 48°,
		+	respectively) than the WT (22°). In addition, the two
		+	transgenic lines had many more tillers per plant (21 and 23,
		+	respectively) than the WT (17 on the average). In
		+	contrast, the whole plant height was very significantly
		+	decreased byOsPIN2over-expression. The transgenic
		+	plant phenotypes were inheritable from T0 (Figure 2f–h) to T2
		+	generations.
		+	At the ripening stage, theOsPIN2-over-expressing
		+	plants showed shorter panicle length, less number of grains per
		+	panicle and lower grain weight per panicle in comparison with
		+	the control. Even though the seed setting rate was
		+	similar between O1, O2 and WT, O1 plants had a 35% increase
		+	in the effective tiller number, leading to a 16% grain yield
		+	increase per plant relative to control plants. O2 plants
		+	showed the same tendency but not significant increase in panicle number and grain yield. OsPIN2over-expression
		+	also decreased the grain length and breadth by 4%–7% and
		+	12%–16%, respectively. The changes in the grain size
		+	lead to 10%–12.5% decreases of 1000-grain weight compared
		+	to WT. Moreover, over-expression of the OsPIN2significantly
		+	decreased the number of adventitious roots and the total root
		+	length by 22%–28%.

← Older revision		Revision as of 13:55, 3 June 2014
Line 33:		Line 33:

	===Expression===		===Expression===
		+	'''Generation of transgenic rice showingOsPIN2 over-expression and phenotypes of transformants'''
		+	Ubiquitin promoter (pUbi) has been long reported as a useful
		+	strong promoter in a variety of applications in gene transfer
		+	studies and drives the gene expression most actively in rapidly
		+	dividing cells (Cornejoet al., 1993)<ref name="pmid:8219091" /> . To investigate the function
		+	ofOsPIN2in rice, we made transgenic rice plants over-expressing the full length of cDNA driven by pUbi. Southern blotting
		+	analysis revealed that they came from two transgenic lines (O1
		+	and O2, respectively) distinct from each other and both had
		+	one copy of the transgene (Figure 1a). Real-time RT-PCR
		+	analyses showed that transcriptional expression ofOsPIN2was
		+	moderate in the root–shoot junctions and roots but very faint in
		+	the leaves of WT (the untransformed plants as control)
		+	(Figure 1b). In both the transgenic lines, OsPIN2had the most
		+	abundant transcripts in the root–shoot junction, and similar
		+	levels in the leaves and roots (Figure 1b). The increases in
		+	OsPIN2transcriptional expression driven by pUbi in these tissues
		+	reached by 3–15 times.

@@ Line 33: / Line 33: @@
 ===Expression===
-'''Generation of transgenic rice showingOsPIN2 over-expression and phenotypes of transformants'''
-Ubiquitin promoter (pUbi) has been long reported as a useful
 There was no putative gene in the insertion site for both O1 and O2. This direct genetic evidence further supported the OsPIN2 contribution to the altered

← Older revision		Revision as of 13:27, 3 June 2014
Line 107:		Line 107:

	Genes homologous to the Arabidopsis PIN sequences are present in genomes throughout the plant kingdom. These include representatives in non-vascular plants such as Physcomitrella; in vascular plants, PIN sequences appear to be ubiquitous. Both before and since the divergence of monocot and dicot plants there have been significant changes in the number and the structure of PINs. However, in dicot plants, the phylogenetic structure of the family has been broadly conserved ( Figure 2a). Both Medicago and potato contain at least five PIN sequences (these data were collected from EST sequencing projects and, accordingly, have the propensity to exclude sequences from mRNA expressed either at a low level or exclusively in certain tissue types). Each of these genes shows similarity to one of the eight PINs of Arabidopsis. Both Medicago and potato contain two genes homologous to AtPIN1 and two genes homologous to the AtPIN3, AtPIN4, AtPIN7 group. Medicago has one gene homologous to AtPIN2. Potato has one gene homologous to AtPIN6. There is additional evidence for such a broadly conserved phylogenetic structure in the PIN genes of soybean (Glycine max). Here, there exist single homologous genes to AtPIN1, AtPIN2, AtPIN5, AtPIN8, and two genes homologous to the AtPIN3, AtPIN4, AtPIN7 cluster of Arabidopsis.		Genes homologous to the Arabidopsis PIN sequences are present in genomes throughout the plant kingdom. These include representatives in non-vascular plants such as Physcomitrella; in vascular plants, PIN sequences appear to be ubiquitous. Both before and since the divergence of monocot and dicot plants there have been significant changes in the number and the structure of PINs. However, in dicot plants, the phylogenetic structure of the family has been broadly conserved ( Figure 2a). Both Medicago and potato contain at least five PIN sequences (these data were collected from EST sequencing projects and, accordingly, have the propensity to exclude sequences from mRNA expressed either at a low level or exclusively in certain tissue types). Each of these genes shows similarity to one of the eight PINs of Arabidopsis. Both Medicago and potato contain two genes homologous to AtPIN1 and two genes homologous to the AtPIN3, AtPIN4, AtPIN7 group. Medicago has one gene homologous to AtPIN2. Potato has one gene homologous to AtPIN6. There is additional evidence for such a broadly conserved phylogenetic structure in the PIN genes of soybean (Glycine max). Here, there exist single homologous genes to AtPIN1, AtPIN2, AtPIN5, AtPIN8, and two genes homologous to the AtPIN3, AtPIN4, AtPIN7 cluster of Arabidopsis.
		+
		+	[[File:Neighbour-joining phylogenetic trees showing the predicted relationship between Arabidopsis AtPINs and (a) dicot (Medicago, soybean and potato) and (b) monocot (rice and wheat) PINs.jpg]]

	To confirm that the changed phenotype of O1 and O2 was		To confirm that the changed phenotype of O1 and O2 was

@@ Line 104: / Line 104: @@
 ===Evolution===
 To confirm that the changed phenotype of O1 and O2 was
 caused by over-expression of OsPIN2in the transgenic plants,