Os06g0665400 - Revision history

Liman: /* Annotated Information */

2016-12-10T12:30:20Z

‎Annotated Information

Liman at 12:30, 10 December 2016

2016-12-10T12:30:00Z

Liman at 12:20, 10 December 2016

2016-12-10T12:20:42Z

Liman at 13:33, 15 October 2016

2016-10-15T13:33:41Z

192.168.72.52 at 10:28, 16 June 2015

2015-06-16T10:28:07Z

Jingtaowu: /* Molecular function of APO2 */

2014-06-08T13:56:22Z

‎Molecular function of APO2

Jingtaowu: /* Molecular function of APO2 */

2014-06-08T13:56:05Z

‎Molecular function of APO2

Jingtaowu: /* References */

2014-06-08T13:54:52Z

‎References

Jingtaowu: /* Function */

2014-06-08T13:54:33Z

‎Function

Jingtaowu: /* References */

2014-06-08T13:53:50Z

‎References

@@ Line 12: / Line 12: @@
 *　Molecular studies revealed that APO1 encodes an F-box protein, an ortholog of　Arabidopsis UNUSUAL FLORAL ORGAN (UFO), which is a positive regulator of class-B genes.
 ===Mutation===
-[[File:Os06g0665400-1.png|right|thumb|400px|'''Figure 1. Phenotypes of apo1.''' '' <ref name="ref1" />.'']]
+[[File:Os06g0665400-1.png|right|thumb|300px|'''Figure 1. Phenotypes of apo1.''' '' <ref name="ref1" />.'']]
 * apo1 showed a number of abnormal phenotypes in the vegetative and reproductive phases. During vegetative growth, apo1 plants produced leaves rapidly, resulting in 1.5-fold more leaves than the wild type at the mature stage. In the reproductive phase, apo1 exhibited remarkable phenotypes in both panicle (inflorescence) architecture and floral organ identity (Figure 1a). At the early stage of panicle development primary branch primordia were produced by distichous phyllotaxy, in contrast to 2/5 spiral phyllotaxy in the wild type (Figure 1b,c).
 * The apo1 mutation also affected floral organ identities. In apo1 flowers, stamens were frequently replaced with lodicules (petals), and carpels were produced indeterminately (Figure 1d–f).
@@ Line 20: / Line 20: @@
 You can also add sub-section(s) at will.
 ==Labs working on this gene==
 * Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan

@@ Line 6: / Line 6: @@
 ===Function===
-[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
+* '''''APO1''''' positively controls spikelet number by suppressing the precocious conversion of inflorescence meristems to spikelet meristems. In addition, '''''APO1''''' is associated with the regulation of the plastchron, floral organ identity, and floral determinacy.
 ===Mutation===
-[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
+[[File:Os06g0665400-1.png|right|thumb|400px|'''Figure 1. Phenotypes of apo1.''' '' <ref name="ref1" />.'']]
 ===Expression ===
-[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
+* APO1 was expressed in apical meristems and the lateral organ primordia throughout development.
 You can also add sub-section(s) at will.

@@ Line 1: / Line 1: @@
-The rice gene  was reported as '''''''''' in 20<ref name="ref1" /> by researchers from China and Japan.
+The rice gene Os06g0665400 was reported as '''''apo1''''' in 2007<ref name="ref1" /> by researchers from Japan.
 ==Annotated Information==
 ===Gene Symbol===
-*''''' <=>
+*'''''Os06g0665400 <=> apo1(t), apo1, SCM2, SCM2/APO1, OsFbox321, Os_F0393
 ===Function===
@@ Line 19: / Line 19: @@
 You can also add sub-section(s) at will.
 ==Labs working on this gene==
+* Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan
 ==References==
 <references>
 * <ref name="ref1">
 </ref>

@@ Line 1: / Line 1: @@
-Please input one-sentence summary here.
+==Annotated Information==
 ===Function===
-Please input function information here.
+[[File:.png|right|thumb|400px|'''''' '' <ref name="ref1" />.'']]
 ===Evolution===
 You can also add sub-section(s) at will.
 ==Labs working on this gene==
 ==References==
-Please input cited references here.
+<references>
 ==Structured Information==
 		 [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 06]]

@@ Line 1: / Line 1: @@
-The temporal and spatial control of meristem identity is a key element in plant development.Through certain research,we found out that the rice aberrant panicle organization 1 (apo1) mutants revealed that APO1 positively controls spikelet number by suppressing the precocious conversion of inflorescence meristems to spikelet meristems.Besides, the rice aberrant panicle organization 2 (apo2) mutant which exhibits small panicles reduces number of primary branches due to the precocious formation of spikelet meristems.
+Please input one-sentence summary here.
-To better understand the molecular mechanisms that regulate inflorescence and flower architecture,To elucidate its molecular function, we isolated the APO1 and AP02 gene using a map-based strategy. And through analyses of phenotypes and gene expression,we revealed that phenotypic analyses of apo1 and floral homeotic double mutants demonstrate that APO1 positively regulates class-C floral homeotic genes, but not class-B genes. Molecular studies revealed that APO1 encodes an F-box protein, an ortholog of Arabidopsis UNUSUAL FLORAL ORGAN (UFO), which is a positive regulator of class-B genes. Over expression of APO1 caused an increase in inflorescence branches and spikelets. As the mutant inflorescences and flowers differed considerably between apo1 and ufo, the functions of APO1 and UFO appear to have diverged during evolution.<ref name="ref2" />
+==Annotated Information==
+===Function===
-In addition,APO2 is identical to previously reported RFL gene, the rice ortholog of the Arabidopsis LEAFY(LFY) gene. Further analysis indicated that APO2/RFL and APO1, the rice ortholog of Arabidopsis UNUSUAL FLORAL ORGANS, act cooperatively to control inflorescence and flower development. The present study revealed functional differences between APO2/RFL and LFY. In particular, APO2/RFL and LFY act oppositely on inflorescence development. Therefore, the genetic mechanisms for controlling inflorescence architecture have evolutionarily diverged between rice (monocots) and Arabidopsis (eudicots).
+Please input function information here.
-Masahiko Maekawa, National Institute for Bioresources, Okayama University, Kurashiki, Okayama 710-0046, Japan.
+===Expression===
-Takeshi Izawa,National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
+===Evolution===
-Kyoko Ikeda,Momoyo Ito, Nobuhiro Nagasawa, Junko Kyozuka and Yasuo Nagato,
+You can also add sub-section(s) at will.
-===References===
+==Labs working on this gene==
-<references>
+Please input related labs here.
-<ref name="ref2">Terao T, Nagata K, Morino K, et al. A gene controlling the number of primary rachis branches also controls the vascular bundle formation and hence is responsible to increase the harvest index and grain yield in rice[J]. Theoretical and applied genetics, 2010, 120(5): 875-893.</ref>
+==References==
-<ref name="ref3">Ikeda K, Ito M, Nagasawa N, et al. Rice ABERRANT PANICLE ORGANIZATION 1, encoding an F‐box protein, regulates meristem fate[J]. The Plant Journal, 2007, 51(6): 1030-1040.</ref>
+==Structured Information==
-</references>
+		 [[Category:Genes]][[Category:Oryza Sativa Japonica Group]][[Category:Japonica Chromosome 06]]

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 As for APO2,it was mapped to a location near the Rice FLORICAULA (RFL) gene (AB005620) on chromosome 4(Kyozuka et al., 1998). Sequencing of RFL in apo2 revealed that apo2-1 has a single base change (C fi A) in the middle of exon2 that causes a premature termination of translation(S210stop). Thus, the apo2-1 protein is presumed to lack theDNA binding domain in the C-terminal region. In contrast,apo2-2 has a T fi A transition in exon1 that causes an amino acid change (L102H) (Figure S3). To confirm that APO2 is identical to RFL, a genomic DNA fragment was introduced into apo2-2. The plants transformed with RFL showed panicles with an increased number of spikelets compared with plants harboring an empty vector, demonstrating that APO2 is RFL .
-The spatial expression pattern of APO2/RFL was previously reported by Kyozuka et al. (1998) and Rao et al. (2008). We re-examined the detailed expression pattern by in situ hybridization. In the vegetative phase, APO2/RFL was expressed in shoot meristems and leaf primordia, in contrast to the result of Kyozuka et al. (1998) who did not detect the expression in the vegetative meristems.Because apo2 mutants exhibited several phenotypes in vegetative phase, the expression in shoot apical meristems would be reasonable. In the reproductive phase, APO2/RFL was expressed in the meristems of primary and secondary branches at primordial and elongating stages , but the expression transiently down regulated from the meristems at stage In5 (Figure 5d). When spikelets were differentiated,APO2/RFL expression was recovered in floral meristems and in primordia of all floral organs, including lodicules, stamens, carpels and ovules. This unique expression pattern during the reproductive phase has not been reported to date. Since the timing of the down-regulation coincided with that of the meristem identity change from inflorescence to spikelet and the mutant inflorescence phenotypes seemed to be independent from those in spikelets/flowers, APO2/RFL may independently function between inflorescence and the spikelet/flower stages.<ref name="ref3" />
+The spatial expression pattern of APO2/RFL was previously reported by Kyozuka et al. (1998) and Rao et al. (2008). We re-examined the detailed expression pattern by in situ hybridization. In the vegetative phase, APO2/RFL was expressed in shoot meristems and leaf primordia, in contrast to the result of Kyozuka et al. (1998) who did not detect the expression in the vegetative meristems.Because apo2 mutants exhibited several phenotypes in vegetative phase, the expression in shoot apical meristems would be reasonable. In the reproductive phase, APO2/RFL was expressed in the meristems of primary and secondary branches at primordial and elongating stages , but the expression transiently down regulated from the meristems at stage In5 (Figure 5d). When spikelets were differentiated,APO2/RFL expression was recovered in floral meristems and in primordia of all floral organs, including lodicules, stamens, carpels and ovules. This unique expression pattern during the reproductive phase has not been reported to date. Since the timing of the down-regulation coincided with that of the meristem identity change from inflorescence to spikelet and the mutant inflorescence phenotypes seemed to be independent from those in spikelets/flowers, APO2/RFL may independently function between inflorescence and the spikelet/flower stages.<ref name="ref1" />
 [[File:wjt3.jpg]]